Proposta modelar de custos ABC na definição do custo-alvo : um estudo na cadeia agronegocial láctea gaúcha

Este trabalho propõe um modelo para definir o custo-alvo com base no sistema de custeamento por atividades, no âmbito da cadeia de agronegócios de leite. Constatado a falta de conhecimento prévio na literatura disponível, foi questionado a contribuição do modelo na gestão de custos interorganizacionais. A fronteira de estudo ateve-se ao setor lácteo. A pesquisa é classificada como um estudo de caso do tipo embedded. E ainda considerando o caráter central da investigação todas as ações apontam nas direções de uma pesquisa-ação. No entanto, considerando os settings de pesquisa e a própria característica dos atores o estudo recorre ao recurso metodológico da etnografia. Entende-se que o enfoque etnográfico empreendido na investigação se emparelha ao recurso da pesquisa-ação. Investido na categoria de participante completo, o pesquisador procedeu adoção da observação conforme suas variantes: descritivas, focais, seletivas e esgotadas. Paralelamente a seqüência observacional, a entrevista com especialistas e a entrevista etnográfica se mostraram promissoras. Apesar dos recursos tecnológicos de registro de informações adotou o diário de campo como principal meio A interpretação e a atribuição de significados foram exercidas durante um processo qualitativo, não requerendo o uso de métodos e técnicas estatísticas. Como o ambiente natural é a fonte direta na captação dos dados e o pesquisador é o instrumento-chave, o procedimento analítico dos dados foi indutivo. O estudo revela-se um avanço no conhecimento, preenchendo uma lacuna existente na literatura sobre agronegócios. A modelagem se delineia favorável em seus resultados, mas requerendo replicações de forma a catalisar a robustez.

Clonagem e expressão do gene que codifica o inibidor de quimotripsina de Erythrina velutina WILLD. - caracterização e avaliação de seu potencial farmacológico

Proteinases are enzymes distributed widely founded in several organisms and perform many different functions, from maintaining homeostasis to the worsening of some diseases such as cancer, autoimmune diseases and infections. The proteins responsible of controlling the action of these enzymes are the inhibitors, that are classified based on their target proteases and are founded since simple organisms, such as bacteria, to higher organisms, such as larger plants and mammals. Plant proteinase inhibitors act by reducing or inactivating the activity of target proteases, thus, these proteins have been studied as potential tools in the treatment of diseases related to protease activities. In this context, an inhibitor of chymotrypsin from Erythrina velutina, called EvCI was previously purified and it was observed that this protein plays in vitro anticoagulant activity and anti-inflammatory activity in in vivo model. Aiming to reduce the environmental impact caused by the purification EvCI in high amounts and to facilitate the process of obtaining this protein, the recombinant chymotrypsin inhibitor from Eryhrina velutina was produced after cloning and expression in Escherichia coli. The bacteria were grown in LB medium and after induction of the expression this material was subjected to procedures for cell lysis and the product was applied on Nickel-affinity column. The proteins adsorbed were digested by thrombin and applied on Chymotrypsin-Sepharose affinity column, obtaining the purified inhibitor, named recEvCI. After electrophoresis, the recombinant inhibitor showed an approximately molecular mass of 17 kDa, and reduced the chymotrypsin and elastase activities in vitro. The recombinant inhibitor was sequenced and was found similar amino acids residues when compared to other inhibitors deposited in the database, with some modifications. recEvCI showed high stability under pH variations and reducing conditions, maintaining its activity around 80%. This protein increased the blood coagulation time in vitro by acting on the intrinsic pathway and did not show cytotoxicity against strains of mouse 3T3 fibroblasts and RAW 264.7 macrophages. recEvCI showed microbicide activity related to release of nitric oxide and consequently the activation of macrophages, futhermore having proinflammatory effects assessed by increased release of TNF-α. These results indicate that recEvCI can be biotechnologically used as a new tool in the control of coagulation-related diseases as well as can be an activating agent of the immune system in immunosuppressed individuals

Análise filogenética e funcional de dois genes de reparo homólogos a AP endonuclease em cana-de-açúcar: ScARP1 e ScARP3

The genome of all organisms constantly suffers the influence of mutagenic factors from endogenous and/or exogenous origin, which may result in damage for the genome. In order to keep the genome integrity there are different DNA repair pathway to detect and correct these lesions. In relation to the plants as being sessile organisms, they are exposed to this damage frequently. The Base Excision DNA Repair (BER) is responsible to detect and repair oxidative lesions. Previous work in sugarcane identified two sequences that were homologous to Arabidopsis thaliana: ScARP1 ScARP3. These two sequences were homologous to AP endonuclease from BER pathway. Then, the aim of this work was to characterize these two sequence using different approaches: phylogenetic analysis, in silico protein organelle localization and by Nicotiana tabacum transgenic plants with overexpression cassette. The in silico data obtained showed a duplication of this sequence in sugarcane and Poaceae probably by a WGD event. Furthermore, in silico analysis showed a new localization in nuclei for ScARP1 protein. The data obtained with transgenic plants showed a change in development and morphology. Transgenic plants had slow development when compared to plants not transformed. Then, these results allowed us to understand better the potential role of this sequence in sugarcane and in plants in general. More work is important to be done in order to confirm the protein localization and protein characterization for ScARP1 and ScARP3

Identificação morfológica de organismos semelhantes à anaplasmataceae em monócitos de surubim híbrido (Pseudoplatystoma reticulatum x P. corruscans) – relato de caso

The aim of this study was to report the occurrence of Anaplasmataceae-like organisms in monocytes from the hybrid surubim catfish. During the hematological evaluation of fish infected by Pseudomonas sp. in a fish farm located in the State of Mato Grosso do Sul we observed into the monocytes the presence of numerous pleomorphic inclusions of various sizes, rough in appearance which presented basophilic staining. These inclusions were similar to elementary bodies, initial bodies and morule of the bacteria from Anaplasmataceae family, often diagnosed in domestic mammals. This is the first report of its occurrence possibly belonging to the family of Anaplasmataceae in cultured fish in Brazil. Additional studies are necessary for molecular characterization of this bacteria, pathogenic potential, life cycle and impact on the intensive production of fish.

Da crise do estado à democracia como alvo: Observações sobre o neoliberalismo a partir de Friedrich Hayek e Milton Friedman

The neoliberalism proclaims the crisis of the State in front of globalization , but, approaching two books taken as basic on this theoretical chain - The road to serfdom, of Friedrich Hayek, and Capitalism and Freedom, of Milton Friedman - to analyze this supposed dualism, the conclusion into which we arrive is another one. Remembering liberal tradition and quickly, later, analyzing critically the workmanships, can be perceived that others are the conflicts really gifts in the current capitalist reality - market versus State et capitalism versus democracy - and, from the understanding on the reading made and the theoretical trajectory of its authors, we may see as the neoliberalism locates itself in relation to these conflicts, which polar regions of these antagonisms privileges, what represents the State for itself, and what it intends as much more global philosophy than economic/politics thinking only

Avaliação do padrão de distribuição de bicos para aplicação de herbicidas: efeitos da altura do alvo nos padrões de distribuição

A avaliação do desempenho de barras de pulverização pode ser feita com base no padrão de distribuição ou deposição do líquido de cada bico individual em mesa de prova. Neste trabalho, compararam-se os padrões de deposição produzidos por diversos bicos em várias alturas. Os resultados mostraram melhor desempenho para os bicos TF e XR, seguido por TJ60-8010, TJ60-8006 e FL. Os bicos TJ60-11006 e TQ mostraram problemas localizados e TK foi considerado o pior entre os testados. Foram propostos modelos para representar as relações entre estatísticas obtidas no padrão e na altura do alvo.

Elaboração e validação de escala diagramática para quantificação da mancha alvo em folhas de acerola

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bioprospecção química da esponja callyspongia vaginalis (Callyspongiidae)

Recently, marine organisms have attracted attention because of the complexity and potent biological activity from your secondary metabolites. Our planet has 80% it surface covered by oceans and seas, therefore, housing a wide number of different forms of life, among them, the sponges. These sessile and filtrating animals, according to numerous researches, come showing like true chemistry factories. The substances from these animals, sometimes show as news targets to therapeutics agents, and some countries has already use them for treatment of some diseases. Further of the secondary metabolites, the polysaccharides of marine origin also have been target of studies, because the presence of the sulfates groups in its molecules. Polysaccharides with differents biological activities have been related in a large number of researches. Actually, many studies show the sponges as source of promising medicine. These studies inspire new researches, because the few number of sponges species studied until now. Because of that, the present work shows the chemistry prospection of the sponge Callyspongia vaginalis. Chromatographic methods in silica gel allowed the isolations of two secondary metabolites: the known β- sitosterol and a ceramide, no reported in the genus Callyspongia, previously. The analysis of the their lipid extracts show different kinds of fatty acids with a variety of chain length (saponifiable fraction), and others metabolites like Lupenone and stigmasterol, also unprecedented in the genus. The Polysaccharide characterization and the elucidation of the secondary metabolites acquired through of chromatography analysis (CC, molecular exclusion) and spectrometric (NMR 1H and 13C, mass, IR), respectively and comparison with literature data

Políticas recentes para arranjos produtivos locais: um olhar sob a lente dos organismos responsáveis pelo apoio no estado do Rio Grande do Norte.

This paper aims to analyze the current support structure ready to Local Productive Arrangements (APLs) in the state of Rio Grande do Norte. To this end, it was done a study of major theories dealing with territorial agglomerations: moving from the pioneering concept of Marshallian industrial district to neo-Schumpeterian concepts coming from the latest innovation systems. In a complementary way, there will be a study of the major reasons that brought the crowded productive activities to a central position in the economic debate and the formation of public policy, seeking to understand what the relationship of this phenomenon with the different approaches of regional development policies which are included in recent years. Finally, it sought to understand how the bodies responsible for supporting clusters in the state of Rio Grande do Norte act in the selection and support of these arrangements, analyzing what the main policies implemented and trying to understand what are the main instruments used to support these clusters in the state

Relação da expressão de fatores de crescimento celular (IGF-1) e (SCF) com fatores prognósticos e o alvo da rapamicina em mamíferos (m-TOR) em mastocitomas cutâneos caninos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Monitoramento de micro-organismos indicadores de higiene em linha de abate de bovinos de um matadouro-frigorífico habilitado à exportação no oeste do Paraná

Indicators microorganisms can be used as a method for evaluating the sanitary quality of food products, indicating the suitability of conditions for obtaining, processing and storage of food. This study sought to evaluate surface contamination of cattle carcasses that come from a slaughter plant under Federal Inspection in western Parana by mesophilic bacteria (AM), Enterobacteriaceae (EB), coliform at 35 degrees C (TC) and E. coli (EC). Surface samples were collected from 25 carcasses, each at 4 different points of slaughter, for non-destructive method. The samples were diluted in decimal scale and incubated in Petrifilm (R) plates. Were found averages of 1.46; 0.3; 0.23 and 0.21 log CFU / cm (2) for AM, EB, CT and EC, respectively. The values to be found were well below those recommended by the decision 471/2001 of the European Union and several other authors surveyed, indicating the effectiveness of the hygienic process industry.