Spectrophotometric determination of diclofenac in pharmaceutical preparations assisted by microwave oven

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Biochemical traits useful for the determination of genetic variation in a natural population of Myracrodruon urundeuva

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Boron influence on concentration of polyols and other sugars in Eucalyptus

Embora a função do elemento Boro (B) nas plantas ainda não tenha sido bem esclarecida, várias hipóteses vêm sido levantadas. Uma das funções atribuídas a esse elemento é a síntese de carboidratos. Com o objetivo de avaliar o efeito do B sobre a síntese de alguns polióis e açúcares em Eucalyptus grandis e híbridos urograndis, estes foram submetidos à situação de suprimento e restrição de B. O experimento foi conduzido em casa de vegetação de acordo com o delineamento de blocos casualizados com cinco repetições, e os elementos nutricionais, assim como o B, foram fornecidos na forma de solução nutritiva. Não se observou diferença significativa nos teores de manitol, sorbitol, myo-inositol e scyllo-inositol e dos açúcares α-glicose e β-glicose entre E. grandis e híbridos. Arabinose foi o único a apresentar maior teor em E. grandis em restrição de B. O efeito da presença do B foi bastante expressivo, mas em suprimento do elemento as plantas apresentaram acréscimo significativo na síntese dos compostos avaliados.

EFFECT OF WATER POTENTIAL ON GERMINATION OF SEEDS OF STYLOSANTHES-GUIANENSIS (AUBL) SW

Effect of water potential on germination of seeds of Slylosanthes guianensis (Aubl.) Sw. To evaluate the water potential effect on germination of S. guianensis two experiments were performed.The first one used osmotic pre-treatment in the imbibition phase and after this period (14 h) the seeds were germinated on filter paper moistened with distilled water. In the second experiment, besides the imbibition phase, seeds were kept in a range of water potentials during all the process. The potentials ranged from 0 to -18 bars, with 3 bars increments, induced by mannitol or by polyethylene glycol. Each treatment was replicated 3 times with 100 seeds per replication. The seeds pre-treated during imbibition had high germination percentage, the highest being the ones in polhyetylene glycol. In the second experiment the polyethylene glycol solutions reduced dramatically the germination percentage in relation to mannitol. From -12 bars on germination ceased in the polyethylene glycol treatments, while in mannitol solution there was 52,67% of germination, in the same water potential.

Antagonism produced by hypertonic nutrient solutions on the depressive effect of carbofuran on isolated guinea pig atria

The action of hyperosmotic nutrient solutions on the depressive effects of carbofuran was studied on spontaneously contracting guinea pig atria. The force and frequency of contraction were recorded using an isotonic lever. Carbofuran 33.0 mu g ml(-1) produced a gradual depressive effect. The time for stabilization of the depressive effect was 6.0+/-2.3 min. After the depressive effect of carbofuran was established, NaCI, mannitol, or urea were introduced into the organ bath to increase the osmolarity of the nutrient solution to about 100 mosmol kg(-1) of water. This resulted in a reversal of the contraction force and frequency to the initial levels. The addition of the osmotic agents at the same concentrations before the addition of 33.0 mu g ml(-1) carbofuran avoided its depressive effect on guinea pig atria. (C) 1996 the Italian Pharmacological Society.

Estresse hídrico induzido por manitol em cultivares de algodão

The main goat of this work was to evaluate the effects induced by mannitol- water stress in the initial growing of cotton plants. The experiment was conducted as a completely randomized design, in factorial design 4 x 5, with 3 replications. Treatments were generated by the combination of four cotton cultivars (LD CV Frego; LD CV 02; FMT 701 and IAC 25) and five levels of water stress (0; -0.3; -0.6, -0.9 and -1.2MPa), obtained with mannitol solutions. Water potentials of -0.9 MPa reduced significantly the rate of cotton plants growth. The decrease of the water potential reduced the production of dry mass of the aerial part of all cultivars and increased the production of root dry mass on -0.6MPa potential. LD CV 02 cultivar exhibited a superior performance under water stress.

INFLUENCIA DO ACRESCIMO DE MANITOL A SOLUCAO NUTRIENTE NO DESEMPENHO MECANICO E NO GRAU DE EDEMA MIOCARDICO DE CORACOES ISOLADOS DE RATOS

Purpose - To analyse the influence of mannitol added to Krebs-Henseleit (KH) solution on the myocardium edema and myocardial function. Methods - Isolated rat heart under isovolumetric contractions studied according to Langendorff's technique were perfused with KH solution at constant flow during 90 min. The coronary perfusion pressure, diastolic and systolic pressures were recorded at every 15 min. At the end of the experiment, myocardium water content was measured in hearts perfused with KH solution (group I, n = 9) and in hearts perfused with KH solution plus 8 mM mannitol (group II, n = 8). These results were compared to non-perfused control heart (n = 9). Results - Myocardial water content was statistically higher in group I (80.8 ± 1.3%) compared to group II (78.1 ± 0.7%) and control group (75.5 ± 0.5%). Systolic arterial pressure was statistically higher in group I (86.2 ± 11.5 mmHg) compared to group II (72.7 ± 21.1 mmHg). There was no difference in the diastolic pressure between the two groups. Coronary perfusion pressure (Pp) increased progressively during the experiment in both groups. However, Pp was lower in group II than in group I. Conclusion - Mannitol added to KH solution significantly attenuates the myocardium edema in the isolated perfused rat heart.

Severe forms of neurocysticercosis: Treatment with albeimdazole

Study of 22 patients with the severe form of neurocysticercosis treated with albendazole (ABZ) administered in 6 different schedules ranging from 15 to 30 mg/kg/day for 21 to 60 days. Dextrochloropheniramine and ketoprofen were the adjuvant drugs Multiple symptoms were observed in 90 9% of patients. Intracranial hypertension was manifested in 90.9%. Hydrocephaly occurred in 86.4%. Evolution was satisfactory in 10 patients, 8 died and 4 had sequelae. Tomographic studies showed the appearance of an isolated IVth ventricle in 9 patients, after ventriculoperitoneal shunt, before ABZ treatment in 3 of them, during in 5 and after treatment in one. Median clinical follow-up duration was 10 months for the patients who died and 3-4 years for survivors. In 3 patients there was an increase in cyst size during the administration of the 15 mg/kg/day ABZ dose, which was not observed in any patient when the 30 mg/kg/day dose was used.

Antibiotic 26-deoxylaidlomycin isolated from Streptomyces sp. Ar386 from Brazilian soil

An actinomycete strain (Ar386) was isolated from the soil of the Araraquara regio, SP, Brazil. The strain, named Streptomyces jacareensis, formed irregular rayed, rugose, grayish-white mycelium with sinuous, branched hyphae carrying rare isolated spores; assimilated glucose, galactose, inositol, ribose, maltose, sucrose, melibiose and starch but not mannitol, rhamnose, arabinose, xylose, lactose and raffinose; and contained LL- diaminopimelic acid in its cell wall. An antibiotic active against Gram- positive bacteria, which was characterized as being 26-deoxylaidlomycin and which may have application against poultry coccidiosis, was isolated from cultures of the strain. This was the first isolation of this antibiotic from a microorganism of the genus Streptomyces and also the first isolation of this antibiotic in Brazil.

Respostas de clones de Eucalyptus grandis e E. grandis x E. urophylla à supressão de boro

The present work had as objective the study of clones of Eucalyptus grandis and E. grandis × E. urophylla under boron suppression on growth in height and diameter, development of symptoms of deficiency, boron content in leaf and polyols production. Plants were cultivated in pots with quartz in a greenhouse using two levels of boron per solution (0 and 0.5 mg L-1 of B). The 32 treatments followed a factorial scheme: 16 × 2 -16 clones and two doses of boron in a randomized block experimental design, with fve repetitions, totaling 160 plots. Plants were evaluated weekly for deficiency symptoms and monthly for height, stem diameter and leaf content of boron in different plant parts. The content of polyols was measured in two occasions: at 162 days and 192 days after starting the experiment. Decrease of growth and quick development of deficiency symptoms 40 days after boron suppression were observed, as well as a decrease of polyols synthesis. Foliar analysis, with boron supplement, resulted in the presence of mannitol and sorbitol in high enough concentration so that the evaluated plants could be considered rich in those polyols. Under boron suppression, boron levels in different portions of branches and stems of plants, as well as the presence of mannitol and sorbitol, indicated a conditional mobility of boron in Eucalyptus.

Chemical composition and antioxidant activity of dried powder formulations of Agaricus blazei and Lentinus edodes

Several mushroom species have been pointed out as sources of antioxidant compounds, in addition to their important nutritional value. Agaricus blazei and Lentinus edodes are among the most studied species all over the world, but those studies focused on their fruiting bodies instead of other presentations, such as powdered preparations, used as supplements. In the present work the chemical composition (nutrients and bioactive compounds) and antioxidant activity (free radical scavenging activity, reducing power and lipid peroxidation inhibition) of dried powder formulations of the mentioned mushroom species (APF and LPF, respectively) were evaluated. Powder formulations of both species revealed the presence of essential nutrients, such as proteins, carbohydrates and unsaturated fatty acids. Furthermore, they present a low fat content (<2 g/100 g) and can be used in low-calorie diets, just like the mushrooms fruiting bodies. APF showed higher antioxidant activity and higher content of tocopherols and phenolic compounds (124 and 770 μg/100 g, respectively) than LPF (32 and 690 μg/100 g). Both formulations could be used as antioxidant sources to prevent diseases related to oxidative stress. © 2012 Elsevier Ltd. All rights reserved.

The tonoplast intrinsic aquaporin (TIP) subfamily of Eucalyptus grandis: Characterization of EgTIP2, a root-specific and osmotic stress-responsive gene

Aquaporins have important roles in various physiological processes in plants, including growth, development and adaptation to stress. In this study, a gene encoding a root-specific tonoplast intrinsic aquaporin (TIP) from Eucalyptus grandis (named EgTIP2) was investigated. The root-specific expression of EgTIP2 was validated over a panel of five eucalyptus organ/tissues. In eucalyptus roots, EgTIP2 expression was significantly induced by osmotic stress imposed by PEG treatment. Histochemical analysis of transgenic tobacco lines (Nicotiana tabacum SR1) harboring an EgTIP2 promoter:GUS reporter cassette revealed major GUS staining in the vasculature and in root tips. Consistent with its osmotic-stress inducible expression in eucalyptus, EgTIP2 promoter activity was up-regulated by mannitol treatment, but was down-regulated by abscisic acid. Taken together, these results suggest that EgTIP2 might be involved in eucalyptus response to drought. Additional searches in the eucalyptus genome revealed the presence of four additional putative TIP coding genes, which could be individually assigned to the classical TIP1-5 groups. © 2013 Elsevier B.V.

Estudo de parâmetros de fermentação na produção de biopolímeros por bactérias isoladas do solo e caracterização química dos grupamentos acetila e piruvato nos biopolímeros obtidos

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE