Transgenic barley expressing a protein-engineered, thermostable (1,3-1,4)-beta-glucanase during germination.

The codon usage of a hybrid bacterial gene encoding a thermostable (1,3-1,4)-beta-glucanase was modified to match that of the barley (1,3-1,4)-beta-glucanase isoenzyme EII gene. Both the modified and unmodified bacterial genes were fused to a DNA segment encoding the barley high-pI alpha-amylase signal peptide downstream of the barley (1,3-1,4)-beta-glucanase isoenzyme EII gene promoter. When introduced into barley aleurone protoplasts, the bacterial gene with adapted codon usage directed synthesis of heat stable (1,3-1,4)-beta-glucanase, whereas activity of the heterologous enzyme was not detectable when protoplasts were transfected with the unmodified gene. In a different expression plasmid, the codon modified bacterial gene was cloned downstream of the barley high-pI alpha-amylase gene promoter and signal peptide coding region. This expression cassette was introduced into immature barley embryos together with plasmids carrying the bar and the uidA genes. Green, fertile plants were regenerated and approximately 75% of grains harvested from primary transformants synthesized thermostable (1,3-1,4)-beta-glucanase during germination. All three trans genes were detected in 17 progenies from a homozygous T1 plant.

Festschrift zur feier des fünfundzwanzigjährigen bestehens der Reallehranstalt zu Essen (Ruhr)

Mode of access: Internet.

Sefer rabot maḥashavot : meḥḳarim ba-Torah uve-ḥokhmah, ba-Miḳra, ba-Talmud uve-midrashim /

"Sefer rishon" ; only one printed?

Gebiete.

1.-19. Aufl. by August Garcke; 20,-22. Aufl. edited by Franz Niedenzu; 23. Aufl.-edited by Konrad von Weihe.

Fasciculus dissertationum medicarum selectiorum : quibus curiosa non minus quam utilia scientiae Apollineae themata diligenter pertractata, & adcurate exposita sistuntur /

Mode of access: Internet.

The German classics of the nineteenth and twentieth centuries : masterpieces of German literature, translated into English /

Chief of the board of translators: Isidore Singer. Cf. "Editorial organization".

Die frühmittelalterliche Porträtmalerei in Deutschland bis zur Mitte des XIII. Jahrhunderts.

Includes bibliographical references and index.

Die Huldar saga.

"Aus den Abhandlungen der K. Bayer. akademie der wiss., I. cl., XX. bd., II. abth."

Pouqueville's Reise durch Morea und Albanien nach Constantinopel, und in mehrere andere Theile des ottomanischen Reichs, in den Jahren 1798, 1799, 1800 und 1801 /

Includes index (citing only volume numbers, not pages).

"Grundriss der romanischen philologie,

"Namen-, sach-, und wortverzeichnis in jedem band."

Catalogus manuscriptorum codicum bibliothecae Uffenbachianæ.

Mode of access: Internet.

Dr. Wilhelm Münscher's Lehrbuch der christlichen Dogmengeschichte.

"2. Hälfte, 2 Abth." [1. Aufl.] ... von Dr. Daniel von Coelln. Nach dessen Tode fortgesetzt von Dr. Ch. Gotthold Neudecker.

Allgemeine Geschichte der christlichen Religion und Kirche /

Advertisements for Perthes: pt. 2, v. 1, p. [1-4] (final group).

Schachbüchlein; oder, Anschauliche darstellung der regeln des schachspieles und der sinnreichsten züge berühmter spieler für anfänger. /

Only part of plate 1 remains; all others missing.

M. Tullius Cicero's Sämmtliche briefe,

On t.-p. of v. 10-12: Vollendet von F.D. Gräter.