Chromatic stability of acrylic resins of artificial eyes submitted to accelerated aging and polishing

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Morphological analysis of the corneal endothelium in eyes of dogs using specular microscopy

Foram estudados 20 olhos de 10 cães sem raça definida, machos e fêmeas com 6 anos de idade. A morfologia das células do endotélio da córnea foi analisada utilizando-se microscópio especular de contato. Foram estudadas as regiões central e periférica da córnea. de cada região da córnea foram realizadas três micrografias. de cada micrografia foram analisadas no mínimo 100 células endoteliais. Foram obtidos os valores do polimegatismo e pleomorfismo. O endotélio corneano de cães caracterizou-se por uma monocamada de células poligonais uniformes em tamanho e forma. A forma predominante das células endoteliais foi hexagonal. O índice de polimegatismo foi 0.22. Este estudo demonstrou que a morfologia das células do endotélio da córnea de cães é semelhante à observada em humanos.

A and B mode ultrasonography in preoperative evaluation of lens and posterior segment of dogs eyes with cataract

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Metacercariae of Diplostomum compactum Lutz, 1928 (Trematoda, Diplostomidae) in the eyes of acara Geophagus brasiliensis Quoy & Gaimard, 1824 (Teleostei, Cichlidae) from Barra Bonita Reservoir - São Paulo, Brazil

Relata-se a ocorrência de metacercárias de Diplostomum compactum (Trematoda: Diplostomidae) infectando Geophagus brasiliensis (Teleostei: Cichlidae) do reservatório de Barra Bonita, rio Tietê, estado de São Paulo, Brasil. As metacercárias foram coletadas no globo ocular, fixadas em solução de AFA e coradas com carmim. As análises morfológicas e morfométricas de cinco espécimes foram realizadas por meio do sistema computadorizado de analise de imagem. Este foi o primeiro registro da ocorrência desse parasito no reservatório de Barra Bonita e também o primeiro registro em acará Geophagus brasiliensis.

A- and B-modes echobiometry in cataractous and noncataractous eyes of English Cocker Spaniel dogs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ultrasonographic and ecobiometric findings in the eyes of adult goats

Conhecer o aspecto e as dimensões de olhos de cabras facilita o uso da ultrassonografia na avaliação de doenças oculares. O objetivo do presente trabalho foi o de avaliar os achados ultrassonográficos e ecobiométricos em olhos de cabras adultas. Ultrassonogramas nos modos A e B foram realizados em 30 caprinos adultos (60 olhos) (n=15 fêmeas intactas e n=15 machos castrados). O exame ultrassonográfico foi realizado após a instilação de colírio anestésico. Gel a base de água foi utilizado sobre o transdutor de 20 MHz posicionado-o de forma longitudinasobre a córneal, até que imagens no modo B estivessem de acordo com os ecos gerados pelo modo A. Utilizou-se análise estatística para se comparar os achados ecobiométricos entre os sexos (p<0,05). As médias com seus respectivos desvios padrões das estruturas oculares em machos e fêmeas foram, respectivamente, 3,46±0,55, 3,33±0,46mm (profundidade da câmara anterior); 8,60±0,34, 8,65±0,39mm (espessura da lente); 11,34±0,61, 11,39±0,66mm (profundidade da câmara vítrea) e 23,43±0,92, 23,39±0.86mm (comprimento axial do bulbo ocular). Não foi observada diferença significativa entre os olhos direito e esquerdo, assim como entre machos e fêmeas (p>0,05). O aspecto ultrassonográfico de olhos de cabras se assemelha com o de outras espécies domésticas e silvestres.

Histopathological findings and detection of parasites in the eyes of dogs infected naturally with Leishmania chagasi

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Changes in the structure and pigmentation of the eyes of honeybee (Apis mellifera L.) queens with the limão mutation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evaluation of a double-setting alpha-tricalcium phosphate cement in eviscerated rabbit eyes

Purpose: To evaluate the macroscopy, microstructure, and tissue reaction of a double-setting a-tricalcium phosphate bone cement used as an intraocular implant in rabbits.Methods: the internal and external surface of the double-setting a-tricalcium phosphate implant was analyzed macroscopically and by scanning electron microscopy. Twelve New Zealand rabbits received 12-mm implants made of double-setting alpha-tricalcium phosphate cement after unilateral evisceration. Clinical evaluation was performed daily for the first 15 days after surgery and at 15-day intervals until the end of the study period. For histopathologic analysis, 3 animals per experimental period were submitted to enucleation at 15, 45, 90, and 180 days.Results: on gross inspection, the external surface of the implant was solid, smooth, and compact. The microarchitecture was characterized by the formation of columns of hexagonal crystals with interconnecting channels forming micropores. No wound dehiscence, signs of infection, or implant extrusion were observed in any animal throughout the study period. Histologic examination revealed the formation of fibrovascular tissue surrounding the implants, and there were signs of minimal integration of the surface limiting the fibrocellular cap with the space previously occupied by the implant.Conclusions: the double-setting alpha-tricalcium phosphate implant behaved as an inert and nonintegratable material. The lack of incorporation of this material by fibrovascular tissue is related to its characteristics of compactness and high resistance.

ALTERATIONS IN THE OMMATIDIA OF THE COMPOUND EYES OF APIS-MELLIFERA EYE-COLOR MUTANT WORKERS .3.

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

COMPARATIVE STUDIES ON PIGMENT GRANULES AND PIGMENT DISTRIBUTION IN THE COMPOUND EYES OF WILD-TYPE AND MUTANT WORKER HONEYBEES .2. AN ULTRASTRUCTURAL ANALYSIS

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Mast cells in the eyes of Calomys callosus (Rodentia : Cricetidae) infected by Toxoplasma gondii

The mast cell is a powerful effector cell for the innate immune system, acting through the secretion of several distinct mediators. Few studies have demonstrated the relationship between mast cells and toxoplasmosis. In this study, mast cells were investigated in two experimental Toxoplasma infections using Calomys callosus (Rodentia: Cricetidae) as the host. Animals were inoculated either intraperitoneally or via the conjunctiva with tachyzoites of Toxoplasma gondii (RH strain) and sacrificed after 5 days or 24 h, respectively. Enucleated eyes were processed for histological and ultrastructural analysis. Neither experimental infection altered the localization of mast cells compared to control eyes, but they did lead to an accumulation in some tissues as well as to their activation. There was a significant increase in the number of mast cells within 5 days and 24 h after infection. The ocular lesions were characterized by the presence of tachyzoites, inflammatory cells and vasodilatation in the iris and retina. In conclusion, mast cells were mobilized in these experimental infections, suggesting that they play an important role in the host inflammatory response after infection with T. gondii.

Use of specular microscopy to determine corneal endothelial cell morphology and morphometry in enucleated cat eyes

The purpose of this study was to investigate the effect of age on endothelial morphology and morphometry in cats. The corneal endothelium was studied using a contact specular microscope. A total of 18 cats (Felis catus Linnaeus, 1758) were evaluated in this study. The subjects were divided into three groups of six cats each in function of age: G1 (1 to 3 months old), G2 (5 to 12 months old), and G3 (24 to 40 months old). The examination presented data as endothelial cell density (ECD), average cell area, corneal thickness, polymegathism, and pleomorphism. Results revealed ECD decrease in corneas of normal cats with age, as well as a corresponding increase in endothelial cell area and pleomorphism. The present work suggests that the endothelial parameters evaluated change with advancing age.

The occurrence of Austrodiplostomum compactum (Lutz, 1928) (Digenea: Diplostomidae) metacercariae in the eyes of loricariid fish (Siluriformes: Osteichthyes: Loricariidae) from Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)