Composição química e qualidade fisiológica de sementes de girassol de plantas submetidas à competição intraespecífica

Avaliou-se a composição química e a qualidade fisiológica de sementes de girassol oriundas de diferentes regiões do capítulo e de plantas-mãe originadas de sementes de alto e baixo vigor que sofreram competição intraespecífica. O delineamento experimental foi em blocos casualizados com quatro repetições, sendo os tratamentos distribuídos em parcelas subdivididas. Nas parcelas avaliaram-se cinco proporções de sementes com alto vigor colocadas na linha de semeadura. Nas subparcelas foram avaliadas as plantas oriundas das sementes de cada nível de vigor e dois tratamentos adicionais. Foi realizada análise relacionando-se as proporções de vigor avaliadas e os terços do capítulo. Avaliou-se a porcentagem de proteínas, lipídeos, carboidratos totais, cinzas, o grau de umidade, o peso de mil sementes e as medidas de emergência (tempos inicial, final e médio de emergência, velocidades média e de emergência, incerteza, sincronia, coeficiente de variação do tempo e emergência), obtidas pelo teste de emergência em areia. Os resultados indicam que: a proporção de vigor das sementes na linha de semeadura interfere no desempenho das plantas e na qualidade das sementes resultantes; a composição química das sementes de girassol variou com a posição das sementes no capítulo; a posição da semente no capítulo interferiu nas variáveis da germinação.

La néolithisation dans la région de Montréal depuis le Sylvicole moyen tardif : apport archéopalynologique

Des preuves archéopalynologiques directes appuient maintenant l’hypothèse d’une lente adaptation horticole durant la néolithisation amérindienne de la région de Montréal. Les sites archéologiques Hector-Trudel (BhFl-1a) de Pointe-du-Buisson et Séminaire de Saint-Sulpice (BjFj-18) dans le Vieux-Montréal ont été retenus pour élaborer une méthodologie archéopalynologique d’étude des sols archéologiques. Cela a permis de caractériser l’impact de la présence humaine sur l'environnement végétal des sites et d’identifier des indices de culture et de gestion de plantes allogènes et indigènes. Un complexe horticole de production à petite échelle de maïs (Zea mays), de tournesol (Helianthus annuus) et de petit tabac (Nicotiana rustica) et une forme de gestion des arbustes à petits fruits sont identifiés au site Hector-Trudel durant le Sylvicole moyen tardif (500 à 1000 A.D.). Ces cultigènes sont aussi identifiés au site du Séminaire pour la fin du Sylvicole supérieur ancien (1200 à 1300 A.D.), dans des proportions toutefois plus importantes, et une activité de gestion forestière au profit des arbres à noix et du tilleul d’Amérique (Tilia americana), reflet des pratiques d’entretien des champs cultivés, témoignent d’une évolution dans les comportements

Femtosecond imaging-mode laser-induced breakdown spectroscopy

Many nonlinear optical microscopy techniques based on the high-intensity nonlinear phenomena were developed recent years. A new technique based on the minimal-invasive in-situ analysis of the specific bound elements in biological samples is described in the present work. The imaging-mode Laser-Induced Breakdown Spectroscopy (LIBS) is proposed as a combination of LIBS, femtosecond laser material processing and microscopy. The Calcium distribution in the peripheral cell wall of the sunflower seedling (Helianthus Annuus L.) stem is studied as a first application of the imaging-mode LIBS. At first, several nonlinear optical microscopy techniques are overviewed. The spatial resolution of the imaging-mode LIBS microscope is discussed basing on the Point-Spread Function (PSF) concept. The primary processes of the Laser-Induced Breakdown (LIB) are overviewed. We consider ionization, breakdown, plasma formation and ablation processes. Water with defined Calcium salt concentration is used as a model of the biological object in the preliminary experiments. The transient LIB spectra are measured and analysed for both nanosecond and femtosecond laser excitation. The experiment on the local Calcium concentration measurements in the peripheral cell wall of the sunflower seedling stem employing nanosecond LIBS shows, that nanosecond laser is not a suitable excitation source for the biological applications. In case of the nanosecond laser the ablation craters have random shape and depth over 20 µm. The analysis of the femtosecond laser ablation craters shows the reproducible circle form. At 3.5 µJ laser pulse energy the diameter of the crater is 4 µm and depth 140 nm for single laser pulse, which results in 1 femtoliter analytical volume. The experimental result of the 2 dimensional and surface sectioning of the bound Calcium concentrations is presented in the work.

Vom Biofilm zur planktonischen Lebensform

Im ersten Teil dieser Dissertation stand die Analyse der Motilitätsentwicklung bei Vertretern der Gattung Methylobacterium im Vordergrund. Diese zu den pink pigmentierten fakultativ methylotrophen Mikroorganismen (PPFMs) gehörenden Prokaryoten sind in der Umwelt weit verbreitet. Besonders häufig besiedeln die Mikroben pflanzliche Oberflächen und können als so genannte Phytosymbionten in einer wechselseitigen Beziehung zu pflanzlichen Organismen stehen. In aquatischer Umgebung können Methylobakterien Flagellen aufweisen. Hierbei handelt es sich um spezielle Fortbewegungsorganellen, die den Mikroben eine aktive Beweglichkeit ermöglichen. Die Ausbildung polarer Einzelflagellen bei Methylobacterium-Zellen in planktonischer Lebensweise konnte unter Anwendung verschiedener mikroskopischer Techniken dokumentiert werden. Quantitative Beweglichkeitsstudien zeigten einen charakteristischen Entwicklungsverlauf, korreliert mit den Wachstumsphasen der Bakterienkulturen und machten deutlich, dass die Motilitätsrate durch Umweltfaktoren, wie z. B. die Nährstoffversorgung, beeinflusst werden kann. Es konnte gezeigt werden, dass die Pflanzen-assoziierten PPFMs in der Lage sind, zwischen einer sessilen und planktonischen Lebensweise zu wechseln und dass sowohl die zelluläre Beweglichkeit als auch die Biofilm-Bildung der Prokaryoten ein reversibles, reaktivierbares Verhalten darstellt. Weiterhin konnte belegt werden, dass die Motilität der epiphytischen Mikroben bezüglich der Besiedelung von Pflanzen, z. B. bei der Ausbreitung auf Keimblatt-Oberflächen von Sonnenblumen (Helianthus annuus), keine zentrale Rolle spielt und eine endophytische Lebensweise unwahrscheinlich ist. Ziel der Arbeit war weiterhin die Charakterisierung und Identifizierung eines aus der Phyllosphäre der Echten Feige (Ficus carica, Standort Griechenland) isolierten Bakterien-Stammes (Mtb. sp. Fc1). Die fakultativ methylotrophe Stoffwechseleigenschaft, sowie die auffällige rötliche Pigmentierung belegen, dass es sich um einen Vertreter der PPFMs handelt. Die Analyse morphologischer, physiologischer und biochemischer Eigenschaften bestätigte in Übereinstimmung mit molekularphylogenetischen Untersuchungen zur Klassifizierung und taxonomischen Einordnung, dass es sich um Pflanzen-assoziierte Mikroben der Gattung Methylobacterium handelt. Analysen der 16S rDNA sowie partieller Sequenzen der für Methylobakterien etablierten Marker-Gene mxaF und gyrB verdeutlichten die phylogenetische Stellung und die evolutionären Beziehungen des Ficus-Isolates. Obwohl enge Verwandtschaftsverhältnisse zu anderen Methylobacterium-Arten ermittelt werden konnten, war eine Identifizierung als valide beschriebene Spezies nicht möglich. Die Resultate legen den Schluss nahe, dass es sich um eine neue, unbeschriebene Spezies der epiphytisch lebenden Methylobakterien handelt.

Comparison of EDTA and EDDS as potential soil amendments for enhanced phytoextraction of heavy metals

Phytoextraction has been proposed as an alternative remediation technology for soils polluted with heavy metals or radionuclides, but is generally conceived as too slow working. Enhancing the accumulation of trace pollutants in harvestable plant tissues is a prerequisite for the technology to be practically applicable. The chelating aminopolycarboxylic acid, ethylene diamine tetraacetate (EDTA), has been found to enhance shoot accumulation of heavy metals. However, the use of EDTA in phytoextraction may not be suitable due to its high environmental persistence, which may lead to groundwater contamination. This paper aims to assess whether ethylene diamine disuccinate (EDDS), a biodegradable chelator, can be used for enhanced phytoextraction purposes. A laboratory experiment was conducted to examine mobilisation of Cd, Cu, Cr, Ni, Pb and Zn into the soil solution upon application of EDTA or EDDS. The longevity of the induced mobilisation was monitored for a period of 40 days after application. Estimated effect half lives ranged between 3.8 and 7.5 days for EDDS, depending on the applied dose. The minimum observed effect half life of EDTA was 36 days, while for the highest applied dose no decrease was observed throughout the 40 day period of the mobilisation experiment. Performance of EDTA and EDDS for phytoextraction was evaluated by application to Helianthus annuus. Two other potential chelators, known for their biodegradability in comparison to EDTA, were tested in the plant experiment: nitrilo acetic acid (NTA) and citric acid. Uptake of heavy metals was higher in EDDS-treated pots than in EDTA-treated pots. The effects were still considered insufficiently high to consider efficient remediation. This may be partly due to the choice of timing for application of the soil amendment. Fixing the time of application at an earlier point before harvest may yield better results. NTA and citric acid induced no significant effects on heavy metal uptake. (C) 2004 Elsevier Ltd. All rights reserved.

A cultura do girassol como estratégia de competitividade para o agronegócio regional e nacional: importância para a agroenergia e a alimentação

Equacionar aquecimento global, escassez de alimentos e a crescente necessidade energética, tornou-se, atualmente, o grande desafio mundial. Existem diversas culturas agrícolas que podem ser exploradas de maneira estratégica e assim colaborar com a solução deste problema. Dentre elas, pode-se destacar a cultura do girassol (Helianthus Annuus). O girassol é uma das quatro maiores culturas oleaginosas no mundo, cultivado com sucesso nos cinco continentes, ocupando uma área de cultivo superior a 22 milhões de hectares. A participação do Brasil nesse montante é inferior a 1%. Acredita-se que essa pequena participação se deva a fatores sócio-econômicos e tecnológicos. Salienta-se, porém que o Brasil, por suas vantagens comparativas naturais e vantagens competitivas construídas possui condições favoráveis para seu desenvolvimento. Diante dos fatos, o objetivo deste trabalho é aprofundar o conhecimento da cadeia produtiva do girassol e através de sua utilização como estratégia de competitividade, avaliar de maneira sistêmica os impactos na matriz agrícola do País. Dentre as inúmeras vantagens dessa cultura, pode-se destacar: características agronômicas, físicas, químicas, organolépticas e versatilidade, que permitem a utilização e otimização dos fatores de produção já disponíveis; época de plantio (adaptabilidade a diferentes condições edafoclimáticas), podendo ser cultivado desde o Rio Grande do Sul até o Estado de Roraima; sistema radicular (raiz pode chegar a dois metros de profundidade), permitindo o melhor aproveitamento dos nutrientes e da água do solo e promovendo a reciclagem de nutrientes; alto teor de óleo nas sementes (30% a 55%) e; alto valor comercial dos co-produtos. Esse conjunto de características é analisado sob a ótica da teoria das vantagens competitivas e das economias de escala e escopo, demonstrando que com inteligência e pragmatismo, a cultura do girassol pode repetir, com vantagens, o que a soja representou para o agronegócio brasileiro.

Regulação da mobilização de lipídios e proteinas por hormônios, fontes de carbono e nitrogênio durante o crescimento pos-germinativo em girassol

The mobilization of food reserves in storage tissues and allocation of their hydrolysis products in the growing axis are critical processes for the establishment of seedlings after germination. Therefore, it is crucial for mobilization of reserves to be synchronized with the growing axis, so that photosynthetic activity can be started before depletion of reserves. For this, integrative approaches involving different reserves, different hydrolysis products and interaction between storage and growing axis tissues, either through hormones or metabolites with signaling role, can contribute greatly to the elucidation of the regulation mechanisms for reserve mobilization. In this study, was hypothesized that hormones and metabolites have different actions on reserve mobilization, and there must be a crossed effect of sugars on the mobilization of proteins and amino acids on lipids and starch mobilization in sunflower seedlings. This study was conducted with seeds of sunflower (Helianthus annuus L.) hybrid Helio 253 using in vitro culture system. Seeds were germinated on Germitest® paper and grown on agar-water 4 g/L without addition of nutrients during 9 days after imbibition (DAI) for growth curve. To verify the effect of metabolites and hormones, seedlings were transferred in the 2nd DAI to agar-water 4 g/L supplemented with increasing concentrations of sucrose or L-glutamine, abscisic acid, gibberellic acid or indolebutyric acid. The results of this study confirm that the mobilization of lipids and storage proteins occurs in a coordinated manner during post-germination growth in sunflower, corroborating the hypothesis that the application of external carbon (sucrose) and nitrogen (L-glutamine) sources can delay the mobilization of these reserves in a crossed way. Moreover, considering the changes in the patterns of reserve mobilization and partition of their products in seedlings treated with different growth regulators, it is evident that the effects of metabolites and hormones must involve, at least in part, distinct mechanisms of action

Infestação de girassol pela lagarta Chlosyne lacinia saundersii em duas épocas de cultivo

Verificou-se o comportamento de cultivares de girassol em relação ao ataque de Chlosyne lacinia saundersii em condições de campo, avaliando-se o número de lagartas por planta, numero de lagartas por metro linear de plantas, porcentagem de plantas atacadas e porcentagem de desfolha. Concluiu-se que: a) os métodos mais adequados para a avaliação da infestação desse inseto foram a porcentagem de plantas atacadas e o número de lagartas por metro linear de plantas; b) as cultivares de girassol menos infestadas pela praga foram 'Cargill-33', 'Contissol', 'Issanka-P, 'PIGB', 'Uruguai' e 'Contissol-621', enquanto 'Contissol-711% 'Contissol-112' e 'Rumano-P4' foram as mais infestadas e, c) a ocorrência de C. lacinia saundersii foi maior na segunda época de cultivo (fevereiro a junho) do que na primeira (outubro a fevereiro).

Co-accumulation of microbial residues and particulate organic matter in the surface layer of a no-till Oxisol under different crops

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Crop type influences soil aggregation and organic matter under no-tillage

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Soil water retention and s index after crop rotation and chiseling

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Least limiting water range in soil under crop rotations and chiseling

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Soybean root growth and yield in rotation with cover crops under chiseling and no-till

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Adubação nitrogenada em cobertura no girassol irrigado nas condições de Cassilândia-MS

Nitrogen is the most required nutrient by plants for exerting important functions in the vegetable metabolism. In sunflower crop, its efficiency causes nutritional disorder, being the nutrient that most limits the yield. Besides, high doses may increase the incidence of pests and diseases, affecting the yield. Hereafter, this experiment was carried out with the aim of evaluating the response of the irrigated sunflower crop (Helianthus annuus L.) submitted to different doses of side dressing nitrogen fertilization applied uncovered nitrogen in small parts. A randomized complete block design, with four replications was used. The constituted by four doses of nitrogen (0, 20, 40, and 80 kg ha(-1)), as urea. Side dressing nitrogen fertilization provides increase in all characteristics studied, and the dose of N of maxim suitable technical efficiency to have a good yield is of 55 kg ha(-1) of N for the cultivation of the sunflower irrigated in the region of Cassilandia, Mato Grosso do Sul State.

Dissimilaridade genética de girassol por meio de caracteres quantitativos

The aim of this study to evaluate by means of quantitative traits, the genetic dissimilarity among sunflower cultivars in different locations, in addition to the agreement between the methods in order to extract lines for future crosses. There were eight sunflower hybrids grown in two locations in northwestern Rio Grande do Sul, mainly with soil type Oxisol. Multivariate methods were used to determine the genetic diversity, using the Mahalanobis distance. Although the different yield between locations and cultivars, methods of grouping agreed among them selves. To obtain segregating populations, regardless of location, the cultivate 'Olisun 5', demonstrated greater potential for hybridization, with major contributions through number of achenes per chapter and height of the insertion section.