Metodología para ejecución y control de calidad para ortofotos, mediante combinación de sensor digital con LIDAR en zonas rurales

La utilización de una cámara fotogramétrica digital redunda en el aumento demostrable de calidad radiométrica debido a la mejor relación señal/ruido y a los 12 bits de resolución radiométrica por cada pixel de la imagen. Simultáneamente se consigue un notable ahorro de tiempo y coste gracias a la eliminación de las fases de revelado y escaneado de la película y al aumento de las horas de vuelo por día. De otra parte, el sistema láser aerotransportado (LIDAR - Light Detection and Ranging) es un sistema con un elevado rendimiento y rentabilidad para la captura de datos de elevaciones para generar un modelo digital del terreno (MDT) y también de los objetos sobre el terreno, permitiendo así alcanzar alta precisión y densidad de información. Tanto el sistema LIDAR como el sistema de cámara fotogramétrica digital se combinan con otras técnicas bien conocidas: el sistema de posicionamiento global (GPS - Global Positioning System) y la orientación de la unidad de medida inercial (IMU - Inertial Measure Units), que permiten reducir o eliminar el apoyo de campo y realizar la orientación directa de los sensores utilizando datos de efemérides precisas de los satélites. Combinando estas tecnologías, se va a proponer y poner en práctica una metodología para generación automática de ortofotos en países de América del Sur. Analizando la precisión de dichas ortofotos comparándolas con fuente de mayor exactitud y con las especificaciones técnicas del Plan Nacional de Ortofotografía Aérea (PNOA) se determinará la viabilidad de que dicha metodología se pueda aplicar a zonas rurales. ABSTRACT Using a digital photogrammetric camera results in a demonstrable increase of the radiometric quality due to a better improved signal/noise ratio and the radiometric resolution of 12 bits per pixel of the image. Simultaneously a significant saving of time and money is achieved thanks to the elimination of the developing and film scanning stages, as well as to the increase of flying hours per day. On the other hand, airborne laser system Light Detection and Ranging (LIDAR) is a system with high performance and yield for the acquisition of elevation data in order to generate a digital terrain model (DTM), as well as objects on the ground which allows to achieve high accuracy and data density. Both the LIDAR and the digital photogrammetric camera system are combined with other well known techniques: global positioning system (GPS) and inertial measurement unit (IMU) orientation, which are currently in a mature evolutionary stage, which allow to reduce and/or remove field support and perform a direct guidance of sensors using specific historic data from the satellites. By combining these technologies, a methodology for automatic generation of orthophotos in South American countries will be proposed and implemented. Analyzing the accuracy of these orthophotos comparing them with more accurate sources and technical specifications of the National Aerial Orthophoto (PNOA), the viability of whether this methodology should be applied to rural areas, will be determined.

Carbohydrate binding and resistance to proteolysis control insecticidal activity of Griffonia simplicifolia lectin II

Griffonia simplicifolia leaf lectin II (GSII), a plant defense protein against certain insects, consists of an N-acetylglucosamine (GlcNAc)-binding large subunit with a small subunit having sequence homology to class III chitinases. Much of the insecticidal activity of GSII is attributable to the large lectin subunit, because bacterially expressed recombinant large subunit (rGSII) inhibited growth and development of the cowpea bruchid, Callosobruchus maculatus (F). Site-specific mutations were introduced into rGSII to generate proteins with altered GlcNAc binding, and the different rGSII proteins were evaluated for insecticidal activity when added to the diet of the cowpea bruchid. At pH 5.5, close to the physiological pH of the cowpea bruchid midgut lumen, rGSII recombinant proteins were categorized as having high (rGSII, rGSII-Y134F, and rGSII-N196D mutant proteins), low (rGSII-N136D), or no (rGSII-D88N, rGSII-Y134G, rGSII-Y134D, and rGSII-N136Q) GlcNAc-binding activity. Insecticidal activity of the recombinant proteins correlated with their GlcNAc-binding activity. Furthermore, insecticidal activity correlated with the resistance to proteolytic degradation by cowpea bruchid midgut extracts and with GlcNAc-specific binding to the insect digestive tract. Together, these results establish that insecticidal activity of GSII is functionally linked to carbohydrate binding, presumably to the midgut epithelium or the peritrophic matrix, and to biochemical stability of the protein to digestive proteolysis.

Toll-related receptors and the control of antimicrobial peptide expression in Drosophila

Insects defend themselves against infectious microorganisms by synthesizing potent antimicrobial peptides. Drosophila has appeared in recent years as a favorable model to study this innate host defense. A genetic analysis of the regulation of the antifungal peptide drosomycin has demonstrated a key role for the transmembrane receptor Toll, which prompted the search for mammalian homologs. Two of these, Toll-like receptor (TLR)2 and TLR4, recently were shown to play a critical role in innate immunity against bacteria. Here we describe six additional Toll-related genes (Toll-3 to Toll-8) in Drosophila in addition to 18-wheeler. Two of these genes, Toll-3 and Toll-4, are expressed at a low level. Toll-6, -7, and -8, on the other hand, are expressed at high levels during embryogenesis and molting, suggesting that, like Toll and 18w, they perform developmental functions. Finally, Toll-5 is expressed only in larvae and adults. By using chimeric constructs, we have tested the capacity of the signaling Toll/IL-1R homology domains of these receptors to activate antimicrobial peptide promoters and found that only Toll and Toll-5 can activate the drosomycin promoter in transfected cells, thus demonstrating specificity at the level of the Toll/IL-1R homology domain. In contrast, none of these constructs activated antibacterial peptide promoters, suggesting that Toll-related receptors are not involved in the regulation of antibacterial peptide expression. This result was independently confirmed by the demonstration that a dominant-negative version of the kinase Pelle can block induction of drosomycin by the cytokine Spaetzle, but does not affect induction of the antibacterial peptide attacin by lipopolysaccharide.

Regulation of Oleoresinosis in Grand Fir (Abies grandis)1 : Differential Transcriptional Control of Monoterpene, Sesquiterpene, and Diterpene Synthase Genes in Response to Wounding

Grand fir (Abies grandis Lindl.) has been developed as a model system for the study of wound-induced oleoresinosis in conifers as a response to insect attack. Oleoresin is a roughly equal mixture of turpentine (85% monoterpenes [C10] and 15% sesquiterpenes [C15]) and rosin (diterpene [C20] resin acids) that acts to seal wounds and is toxic to both invading insects and their pathogenic fungal symbionts. The dynamic regulation of wound-induced oleoresin formation was studied over 29 d at the enzyme level by in vitro assay of the three classes of synthases directly responsible for the formation of monoterpenes, sesquiterpenes, and diterpenes from the corresponding C10, C15, and C20 prenyl diphosphate precursors, and at the gene level by RNA-blot hybridization using terpene synthase class-directed DNA probes. In overall appearance, the shapes of the time-course curves for all classes of synthase activities are similar, suggesting coordinate formation of all of the terpenoid types. However, closer inspection indicates that the monoterpene synthases arise earlier, as shown by an abbreviated time course over 6 to 48 h. RNA-blot analyses indicated that the genes for all three classes of enzymes are transcriptionally activated in response to wounding, with the monoterpene synthases up-regulated first (transcripts detectable 2 h after wounding), in agreement with the results of cell-free assays of monoterpene synthase activity, followed by the coordinately regulated sesquiterpene synthases and diterpene synthases (transcription beginning on d 3–4). The differential timing in the production of oleoresin components of this defense response is consistent with the immediate formation of monoterpenes to act as insect toxins and their later generation at solvent levels for the mobilization of resin acids responsible for wound sealing.

Common endocrine and genetic mechanisms of behavioral development in male and worker honey bees and the evolution of division of labor.

Temporal polyethism is a highly derived form of behavioral development displayed by social insects. Hormonal and genetic mechanisms regulating temporal polyethism in worker honey bees have been identified, but the evolution of these mechanisms is not well understood. We performed three experiments with male honey bees (drones) to investigate how mechanisms regulating temporal polyethism may have evolved because, relative to workers, drones display an intriguing combination of similarities and differences in behavioral development. We report that behavioral development in drones is regulated by mechanisms common to workers. In experiment 1, drones treated with the juvenile hormone (JH) analog methoprene started flying at significantly younger ages than did control drones, as is the case for workers. In experiment 2, there was an age-related increase in JH associated with the onset of drone flight, as in workers. In experiment 3, drones derived from workers with fast rates of behavioral development themselves started flying at younger ages than drones derived from workers with slower rates of behavioral development. These results suggest that endocrine and genetic mechanisms associated with temporal polyethism did not evolve strictly within the context of worker social behavior.

Insect prevention and control in plants processing dry milk /

Cover title.

Insect control by radiation and radiosotopes : a literature search /

This bibliography contains one hundred and one selected references on the uses of radiation and radioisotopes in entomology. These references were selected from the scientific literature published since 1961. An index to the insects studied is included.

Methods of insect control.

V. 1: 2d ed., rev., 1937-1940 with 1942 printing; v. 2: 3rd edition-revised, copyright 1938, 1940, 1942 with 1944 printing.

A systems analysis view of longitudinal flying qualities

"Flight Control Laboratory. Contract no. AF33(616)-5661. Project no. 1365-13553."

Toward integrated control; proceedings.

Cover title.

Pathogens and microbial control of North American forest insect pests /

"August 1998" -- Cover.

Summary report on VFR general aviation flight plan flying, fiscal year 1959.

Mode of access: Internet.

Summary report on VFR general aviation flight plan flying, fiscal year 1961.

Mode of access: Internet.

Biological control of the oriental fruit fly (Dacus dorsalis Hendel) and other fruit flies in Hawaii,

"Literature cited": p. 100-102.