Posibilidades de manipulación del fotoperíodo durante el engorde intensivo de Dorada (Sparus aurata)

Doctorado en Veterinaria

Dilution and dispersión of Gran Canaria desalinitation plant brine discharge: influence on seagrass meadows and potential corrective measures

Programa de doctorado en oceanografía

Ictus isquémico en la población de las Islas Canarias y su asociación con los genes de la metilentetrahidrofolato reductasa, del sistema renina-agiotensina y de las paroxinasas 1 y 2

Programa de doctorado: Avances en medicina interna

STAR splicing mutations cause the severe phenotype of lipoid congenital adrenal hyperplasia: insights from a novel splice mutation and review of reported cases

OBJECTIVE The steroidogenic acute regulatory protein (StAR) transports cholesterol to the mitochondria for steroidogenesis. Loss of StAR function causes lipoid congenital adrenal hyperplasia (LCAH) which is characterized by impaired synthesis of adrenal and gonadal steroids causing adrenal insufficiency, 46,XY disorder of sex development (DSD) and failure of pubertal development. Partial loss of StAR activity may cause adrenal insufficiency only. PATIENT A newborn girl was admitted for mild dehydration, hyponatremia, hyperkalemia and hypoglycaemia and had normal external female genitalia without hyperpigmentation. Plasma cortisol, 17OH-progesterone, DHEA-S, androstendione and aldosterone were low, while ACTH and plasma renin activity were elevated, consistent with the diagnosis of primary adrenal insufficiency. Imaging showed normal adrenals, and cytogenetics revealed a 46,XX karyotype. She was treated with fluids, hydrocortisone and fludrocortisone. DESIGN, METHODS AND RESULTS Genetic studies revealed a novel homozygous STAR mutation in the 3' acceptor splice site of intron 4, c.466-1G>A (IVS4-1G>A). To test whether this mutation would affect splicing, we performed a minigene experiment with a plasmid construct containing wild-type or mutant StAR gDNA of exons-introns 4-6 in COS-1 cells. The splicing was assessed on total RNA using RT-PCR for STAR cDNAs. The mutant STAR minigene skipped exon 5 completely and changed the reading frame. Thus, it is predicted to produce an aberrant and shorter protein (p.V156GfsX19). Computational analysis revealed that this mutant protein lacks wild-type exons 5-7 which are essential for StAR-cholesterol interaction. CONCLUSIONS STAR c.466-1A skips exon 5 and causes a dramatic change in the C-terminal sequence of the protein, which is essential for StAR-cholesterol interaction. This splicing mutation is a loss-of-function mutation explaining the severe phenotype of our patient. Thus far, all reported splicing mutations of STAR cause a severe impairment of protein function and phenotype.

Hombres, poder y conflicto : Estudios sobre la frontera colonial sudamerica y su crisis

Tabla de contenidos: Nota introductoria / Emir Reitano, Paulo Possamai. Del Tajo al Amazonas y al Plata. Las repercusiones atlánticas de las 12 guerras entre las coronas española y portuguesa en la Edad Moderna / Juan Marchena Fernández. El presidio de Buenos Aires entre los Habsburgo y los Borbones: el ejército regular en la frontera sur del imperio español / Carlos María Birocco. Los soldados indígenas del Rey Católico: los misioneros en las guerras por la Colonia del Sacramento / Paulo César Possamai. Ataque de la flota combinada anglo portuguesa a la Colonia del Sacramento.El hundimiento del navío Lord Clive (1763) / Marcelo Díaz Buschiazzo. Travessías difíceis: Portugal, Colónia do Sacramento e o projeto Montevidéu (1715-1755) / Victor Hugo Abril. Beresford e D. Joao VI ? Uma inesperada confluencia / Fernando Dores Costa. La guerra: una situación límite. Una aproximación al tema: Batalla de India Muerta, noviembre 1816 / Juan Carlos Luzuriaga. Fortalezas imperiais: Arquitetura e cotidiano (Fronteira Oeste da América Portuguesa, século XVIII) / Otávio Ribeiro Chaves. Resistência e cotidiano da tropa militar do presídio de Miranda: Aspectos da defesa da fronteira sul da capitania de Mato Grosso (1797-1822) / Bruno Mendez Tulux. Os índios Payaguá: guerra e comércio na fronteira oeste da América portuguesa / Maria De Jesus Nauk. De Yatay a Cerro- Corá. Consenso e Dissenso na resistência militar paraguaia / Mario Maestri. Extraños en los confines del imperio: los portugueses ante la corona española en el Río de la Plata / Emir Reitano. Incidências da guerra en uma fronteira imperial: Rio Grande de Sao Pedro (1750-1825) / Helen Osorio. Armas y control. El ?negro delito de la deserción? en la Banda Oriental (1811-1816) / Daniel Fessler. Cruzar fronteiras, conectar mundos. As missoes austrais na pampa bonaerense (Século XVIII) / María Cristina Martins. Las guerras coloniales en la historiografía uruguaya de orientación nacionalista / Tomás Sansón. Las estatuas al Almirante Brown y la ?construcción de la Nación Argentina? / Diego Téllez Alarcia.

Phytoextraction of heavy metals from mine soils using hyperaccumulator plants.

Phytoextraction is an environmental-friendly and cost-effective technology that uses metal hyperaccumulator plants to remove heavy metals from soils. The metals are absorbed by the roots, transported and accumulated in the aerial parts of the plants, which can be harvested and eliminated. The aim of this work was to study some hyperaccumulator species that could be useful to decontaminate mine soils and also to investigate the bioavailability and uptake of these metals by plants with the addition of organic amendments. Pot experiments were performed with soil samples collected from two mining areas in the north of Madrid, where there was an intense mining activity more than 50 years ago. Three species (Thlaspi arvense, Brassica juncea and Atriplex halimus) were grown under controlled conditions in pots filled with contaminated soils mixed with 0 Mg, 30 Mg and 60 Mg per hectare of two different organic amendments: a commercial compost made of pine bark, peat and wood fiber and other made of horse and sheep manure and wood fiber. Plants were harvested at the end of their crop cycle and were digested in order to measure metal concentration (Zn, Cu and Cd) in roots and shoots. Highest plant metal concentration was observed in pots treated with pine bark amendment and with pure soil due to an increase in metal bioavailability with decreasing pH. Also in those treatments the total plant biomass was lower, even some plants could not germinate. On the contrary, there was a lower metal concentration in plant tissues of pots with manure because its higher pH whereas plant growth was significantly larger so there was an incresing amount of metals removed from soil by plants. Comparing the three species results indicate a higher total metal uptake in A. halimus than B. juncea and T. arvense. In conclusion, results show that pH affects metal bioavailability and uptake by hyperaccumulator plants. Addition of organic amendments could be a successful technique for stabilization of metals in contaminated soils.

Libro del bien del estado religioso / compuesto en latin por el Padre Hieronymo Plati dela Compañia de Iesus ; traduzido en romance por el P. Francisco Rodriguez de la mesma Compañia ...

Sign.: [calderón]4, 2[calderón]8, 2*8, A-Z8, 2A-2X8, 2Y-2Z4, 3A-3Y8, 3Z10, A8

Libro del bien del estado religioso / compuesto en latin por el Padre Hieronymo Plati dela Compañia de Iesus ; traduzido en romance por el P. Francisco Rodriguez de la mesma Compañia ...

Sign.: [calderón]4, 2[calderón]8, 2*8, A-Z8, 2A-2X8, 2Y-2Z4, 3A-3Y8, 3Z10, A8