679 resultados para Fermentação submersa
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Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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The objective this work was to evaluate the degradation, fermentation and kinetics of passage rumen fluid en sheep fed diets containing different carbohydrate associated with the oil. Four rumen cannulated sheep were allotted in 4 x 4 latin square. The treatments consisted of diets with high neutral detergent soluble fiber and diets with high starch associated or not with 4,2% of oil. Incubation times were 3, 6, 12, 24, 48, 72, 96 and 120 hours and ruminal fluid was collected at 0, 2, 4, 6, 8, 10, 12, and 24 hours after feeding. Diets high neutral detergent fiber soluble resulted in greater degradation of dry matter, crude protein, neutral detergent fiber soluble and starch, and higher pH values, butyric acid production, dilution rate and ruminal recycling compared with diets high starch content. The inclusion of 4,2% oil the different carbohydrate sources had no influence on the fermentation kinetics and degradation of the fiber. High content of neutral detergent soluble fiber in the diet favors the ruminal fermentation compared to the high starch content.
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Pós-graduação em Agronomia (Produção Vegetal) - FCAV
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Recently there is a great quest of producing alcohol from starchy resources, replacing the sugar cane. The most common starchy sources are cassava, maize and sweet potatoes and a lot of research are been realized with excellent results. In this work it was evaluated the influence of the concentration of dry matter on the enzymatic hydrolysis process of starch from sweet potato for ethanol production. Through the sweet potato was produced a flour using a low-cost method and easy operation equipments. The sweet potato flour was characterized physical and chemically and from these results was prepared the treatments for enzymatic hydrolysis. The experimental design considered as independent variable the dry matter concentration of the sweet potato flour in 3 levels; 10, 15 and 20% in the formulation of suspensions. The other variables were keeping constant being: temperature in the 1° hydrolysis step of 90°C and time of 2 hours; temperature in the 2° saccharification step of 60°C and time of 17 hours. The hydrolysates obtained at the three assays were transferred to six liter enlerynmeyer and inoculated with a biologic catalyst, Saccharomyces, dehydrated yeasts of Saccharomyces cerevisiae CAT 1, at a rate of 5% in weight. The flasks were placed in a shaker type orbital with controlled temperature of 30°C during a time of 15 hours. The initial reducer sugars concentration and respective ethanol concentrations in wine were: 11.2% glucose and 2.16% ethanol in the suspension with 10% of dry matter; 13.5% glucose and 4.39% ethanol with 15% and 17.5% glucose and 6.03% ethanol in suspension with 20% of dry matter. ix The results showed that the higher percentage of dry matter carried out to higher sugar yield in hydrolyzed. It was possible observed that products quality improved with a higher concentration of dry matter
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Pós-graduação em Microbiologia Agropecuária - FCAV
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Pós-graduação em Agronomia (Horticultura) - FCA
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Amilases e proteases constituem um dos principais grupos de enzimas industriais pelo seu amplo espectro de aplicações biotecnológicas. Elas podem ser obtidas a partir de fontes microbianas e com altos rendimentos por processos de fermentação em estado sólido (FES). Conhecer as características bioquímicas das enzimas é fundamental para adequação aos processos industriais. O objetivo do trabalho foi determinar a melhor temperatura para atividade das enzimas amilase e protease de Rhizopus oligosporus obtidas por fermentação em estado sólido utilizando farelo de trigo como substrato. Os melhores valores para atividade amilolítica e proteolítica foram obtidos nas temperaturas de 55 - 65 °C e de 50 - 60 °C, respectivamente. Estes resultados sugerem que as enzimas estudadas podem ser utilizadas em processos que empregam elevadas temperaturas.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)