Goethe und Schopenhauer; ein beitrag zur entwicklungsgeschichte der Schopenhauerschen philosophie ...

Mode of access: Internet.

Improving the function of islet and beta-cell grafts

Background: Human islet transplantation would offer a less invasive and more physiological alternative than whole pancreas transplantation and insulin injections respectively for the treatment of diabetes mellitus if islet graft survival can be improved. Initial recipient post-transplant insulin independence declines to <10% after 5 years. Factors contributing to graft failure include enzymatic disruption of the islet microenvironment during isolation, diabetogenic effects of immunosuppressants and metabolic stress resulting from slow revascularisation. Aims: To investigate the effect of co-culture in both static (SC) and rotational culture (RC) of BRINBDII beta-cells (Dl1) and human umbilical vein endothelial cells (HUVEC) on Dl1 insulin secretion; and the effect of a thiazolidinedione (TZD) on DII function and HUVEC proliferation. To assess the effect of culture media, SC, RC and a TZD on human islet morphology, insulin secretion and VEGF production. To initiate in vivo protocol development for assessment of revascularisation of human islet grafts. Methods: D11 cells were cultured +/-TZD and co-cultured with HUVEC +/-TZD in SC and RC. Dl1 insulin secretion was induced by static incubation with low glucose (1.67mM), high glucose (l6.7mM: and high glucose with 10mM theophylline (G+T) and determined by ELISA. HUVEC were cultured +/-TZD in SC and RC and proliferation was assessed by ATP luminescence assay and VEGF ELISA. D II and HUVEC morphology was determined by immunocytochemistry. Human islets were cultured in SC and RC in various media +/-TZD. Insulin secretion was determined as above and VEGF production by fluorescence immunocytochemistry (FI) and ELISA. Revascularisation of islet grafts was assessed by vascular corrosion cast and FI. Results: Dll cultures showed significantly increased insulin secretion in response to 16.7mM and G+T over basal; this was enhanced by RC and further improved by adding 10mM TZD. Untreated Dll/HUVEC co-cultures displayed significantly increased insulin secretion in response to 16.7mM and G+T over basal, again enhanced by RC and improved with 10mM TZD. 10mM TZD significantly increased HUVEC proliferation over control. Human islets maintained in medium 199 (mI99) in SC and RC exhibited comparable maintenance of morphology and insulin secretory profiles compared to islets maintained in RPMI, endothelial growth media and dedicated islet medium Miami# I. All cultures showed significantly increased insulin secretion in response to 16.7mM and G+T over basal; this was enhanced by RC and in certain instances further improved by adding 25mM TZD. TZD increased VEGF production and release as determined by ELISA. Post-implant vascular corrosion casts of mouse kidneys analysed by x-ray micro tomography indicates a possible TZD enhancement of microvessel growth via VEGF upregulation. Conclusions: D II /HUVEC co-culture in SC or RC does not alter the morphology of either cell type and supports D 11 function. TZD improves 0 I I and D I I/HUVEC SC and RC co-culture insulin secretion while increasing HUVEC proliferation. Human islet RC supports islet functional viability and structural integrity compared to SC while the addition of TZD occasionally further improves secretagogue induced insulin secretion. Expensive, 'dedicated' islet media showed no advantage over ml99 in terms of maintaining islet morphology or function. TZD upregulates VEGF in islets as shown by ELISA and suggested by x-ray micro tomography analysis of vascular corrosion casts. Maintenance of islets in RC and treatment with TZD prior to transplant may improve the functional viability and revascularisation rate of islet grafts.

Virtual Instruments – Functional Model, Organization and Programming Architecture

∗ Thematic Harmonisation in Electrical and Information EngineeRing in Europe,Project Nr. 10063-CP-1-2000-1-PT-ERASMUS-ETNE.

A helykötődés és a környezetvédő viselkedés közötti összefüggések vizsgálata turisztikai kontextusban

A személy-környezet összeillés elméletei szerint az emberek viselkedésmintázata és a specifi kus környezeti feltételek között összefüggés áll fenn (Dúll, 1998). Ahogyan Gump (1990) rámutat, két ember hasonlóbban viselkedik egy adott helyen, mint ugyanazon ember két eltérő helyen. Brighem (1991) mindezt azzal magyarázza, hogy az egyén és a környezet között kialakuló interakció során illeszkedés alakul ki az egyén céljai és a környezet elrendezése között. A fi zikai környezet felépítése hatással van a társas interakciók kibontakozásának lehetőségére is, ezáltal befolyásolják az ott élők megküzdési stratégiáit (Evans et al., 1996, in: Dúll, 2009). Az interakciókat gátló helyek ennek megfelelően a személyek közötti kommunikáció hiányához, egymástól való elidegenedéshez vezethetnek. Az így kialakuló kollektív szemlélet hiánya számos, fenntarthatósági szempontból fontos viselkedés gátját képezheti (például javak megosztott igénybevétele, közösségi javak felelős használata). Mindez magyarázatul szolgálhat olyan esetekben is, ahol azt láthatjuk, hogy ugyanazon személy eltérő fogyasztási és viselkedési mintákat követ más-más helyszínen. Turisztikai szempontból azonban fontosabb annak vizsgálata, hogy egy adott desztináció milyen hatással van az odaérkező vendégek viselkedésére, módosítja-e azt, és ha igen, milyen irányba és miért.

“Ainda as ‘Reflexões’ sobre a pronunciação no séc. XVIII português”

Na sequência de trabalhos anteriores, analisam-se aqui algumas das reflexões de natureza fonético-fonológica presentes na obra de Francisco José Freire, Reflexões sobre a língua portuguesa, avaliando a sua contribuição para o conhecimento da língua portuguesa no séc. XVIII.

Low-power high-performance SAR ADC design with digital calibration techniques

This dissertation presents the design of three high-performance successive-approximation-register (SAR) analog-to-digital converters (ADCs) using distinct digital background calibration techniques under the framework of a generalized code-domain linear equalizer. These digital calibration techniques effectively and efficiently remove the static mismatch errors in the analog-to-digital (A/D) conversion. They enable aggressive scaling of the capacitive digital-to-analog converter (DAC), which also serves as sampling capacitor, to the kT/C limit. As a result, outstanding conversion linearity, high signal-to-noise ratio (SNR), high conversion speed, robustness, superb energy efficiency, and minimal chip-area are accomplished simultaneously. The first design is a 12-bit 22.5/45-MS/s SAR ADC in 0.13-μm CMOS process. It employs a perturbation-based calibration based on the superposition property of linear systems to digitally correct the capacitor mismatch error in the weighted DAC. With 3.0-mW power dissipation at a 1.2-V power supply and a 22.5-MS/s sample rate, it achieves a 71.1-dB signal-to-noise-plus-distortion ratio (SNDR), and a 94.6-dB spurious free dynamic range (SFDR). At Nyquist frequency, the conversion figure of merit (FoM) is 50.8 fJ/conversion step, the best FoM up to date (2010) for 12-bit ADCs. The SAR ADC core occupies 0.06 mm2, while the estimated area the calibration circuits is 0.03 mm2. The second proposed digital calibration technique is a bit-wise-correlation-based digital calibration. It utilizes the statistical independence of an injected pseudo-random signal and the input signal to correct the DAC mismatch in SAR ADCs. This idea is experimentally verified in a 12-bit 37-MS/s SAR ADC fabricated in 65-nm CMOS implemented by Pingli Huang. This prototype chip achieves a 70.23-dB peak SNDR and an 81.02-dB peak SFDR, while occupying 0.12-mm2 silicon area and dissipating 9.14 mW from a 1.2-V supply with the synthesized digital calibration circuits included. The third work is an 8-bit, 600-MS/s, 10-way time-interleaved SAR ADC array fabricated in 0.13-μm CMOS process. This work employs an adaptive digital equalization approach to calibrate both intra-channel nonlinearities and inter-channel mismatch errors. The prototype chip achieves 47.4-dB SNDR, 63.6-dB SFDR, less than 0.30-LSB differential nonlinearity (DNL), and less than 0.23-LSB integral nonlinearity (INL). The ADC array occupies an active area of 1.35 mm2 and dissipates 30.3 mW, including synthesized digital calibration circuits and an on-chip dual-loop delay-locked loop (DLL) for clock generation and synchronization.

El exceso de confianza y el statu quo dentro de la estructuración de capital: una perspectiva de las finanzas corporativas del comportamiento

El presente artículo, presenta un análisis de las decisiones de estructuración de capital de la compañía Merck Sharp & Dome S.A.S, desde la perspectiva de las finanzas comportamentales, comparando los métodos utilizados actualmente por la compañía seleccionada con la teoría tradicional de las finanzas, para así poder evaluar el desempeño teórico y real. Incorporar elementos comportamentales dentro del estudio permite profundizar más sobre de las decisiones corporativas en un contexto más cercano a los avances investigativos de las finanzas del comportamiento, lo cual lleva a que el análisis de este artículo se enfoque en la identificación y entendimiento de los sesgos de exceso de confianza y statu quo, pero sobre todo su implicación en las decisiones de financiación. Según la teoría tradicional el proceso de estructuración de capital se guía por los costos, pero este estudio de caso permitió observar que en la práctica esta relación de costo-decisión está en un segundo lugar, después de la relación riesgo-decisión a la hora del proceso de estructuración de capital.

Estudio sobre la viabilidad de la implementación del jurado de conciencia en el ordenamiento colombiano

El objeto del presente trabajo final de grado es analizar la viabilidad de la implementación de la figura del jurado de conciencia en el sistema penal colombiano, que fue suprimida mediante el Decreto 1861 de 1989. A su vez, hacer un análisis de derecho comparado con el fin de determinar los aspectos positivos y negativos de la implementación de los jurados en otros sistemas jurídicos. El resultado del análisis propuesto pretende determinar si es procedente la implementación de los jurados de conciencia en Colombia.

Caminhos da crítica textual Da filologia à interdisciplinaridade: o caso das edições dos Sermões de Padre António Vieira

The present paper discusses the problem of the plurality of the text, as a cultural object, and its implications for the textual criticism activity. Starting from the fact that the nature of the textual object, especially in the case of ancient texts and literary texts, requires the editor to call into play very diverse knowledge – among which stands out the linguistic knowledge, but also the knowledge of the work, its characteristics and context – we advocate that a «philological» approach, multi and interdisciplinary, of the text editing is still needed, desirably carried out by teams of experts in different scientific fields. We exemplify, among others,with the case of the editions of the Sermões, by Vieira.

"A ‘terminologia açucareira’ em dicionários brasileiros da língua portuguesa

Este artigo trata da terminologia açucareira e do seu registo em dicionários brasileiros da segunda metade do século XIX: o «Vocabulario Brazileiro» (1853) e o «Diccionario de Vocabulos Brasileiros» (1889)

Prefácio

O presente texto versa sobre a situação actual da língua portuguesa em Angola e serve de prefácio à obra de Márcio Undolo sobre este tema.

Ortografistas e “Bons Autores”: O Papel da Auctoritas na Definição do Cânon Ortográfico de Setecentos

A conceção instrumental de Gramática – fortemente conservadora e normativa, com origem nos gramáticos Alexandrinos e que se prolongaria até ao séc. XX – prolongou também o papel de relevo dos chamados “bons autores” na definição do cânon gramatical, incluindo a ortografia, o que se justifica atendendo a que, além da regulamentação do comportamento linguístico, esta conceção instrumental compreendia também o acesso aos textos literários. No entanto, à semelhança dos próprios gramáticos e ortografistas, estes “bons autores”, entre os quais se destaca Vieira, primam pela irregularidade e pela tendência para a constituição de ortografias individuais. A partir da prática de alguns dos autores mais relevantes na literatura metalinguística de Setecentos, em particular João de Morais Madureira Feijó (1688-1741) e Francisco José Freire (1719-1773), analisa-se o papel de auctoritates dos “bons autores” no estabelecimento do cânon ortográfico da época e a forma como este papel começaria a alterar-se, particularmente em Madureira Feijó, antecipando, assim, o seu declínio.

Da antiga à nova Filologia: práticas de edição de textos modernos

O presente texto aborda a natureza da actividade filológica do editor, a relação entre as práticas de edição e os diferentes tipos de texto, bem como o papel das novas tecnologias na filologia editorial.