Determinação da Biodisponibilidade da Lisina Sulfato e Lisina HCl com Frangos de Corte

Com o objetivo de determinar a biodisponibilidade de duas fontes de lisina (lisina HCl e lisina sulfato), por intermédio de um ensaio de crescimento, foram alojados em um galpão de alvenaria com 56 boxes 840 pintos de corte machos com um dia de idade. Duas dietas basais foram formuladas para atender as exigências nutricionais das aves nas fases inicial e crescimento, deficientes apenas em lisina e suplementadas em 0,08; 0,16; e 0,24% pelas duas fontes de lisina. As variáveis avaliadas foram: ganho de peso, consumo de ração, conversão alimentar, rendimento de carcaça, rendimento de perna, rendimento de peito, rendimento de filé e porcentagem de gordura abdominal. Com os dados obtidos foram estimadas equações de regressão linear múltipla e, usando os coeficientes de regressão destas, foi determinada a biodisponibilidade da lisina sulfato em relação a lisina HCl, padronizada como 100% disponível. As equações obtidas que melhor estimaram a biodisponibilidade das lisinas foram Y = 544,72 + 439,62 X1 + 475,84 X2, R² = 0,90, para ganho de peso de 01 a 21 dias de idade, Y = 1824,63 + 1469,18 X1 + 1381,33 X2, R² = 0,85, para ganho de peso de 01 a 42 dias de idade, Y = 1,9623 - 0,9043X1--1,0235 X2, R² = 0,83, para conversão alimentar de 01 a 21 dias de idade, Y = 0,3766 + 0,5320 X1 + 0,4986 X2, R² = 0,88, para peso de peito aos 42 dias de idade e Y = 0,2565 + 0,4685X1 + 0,4300 X2, R² = 0,92, para peso de filé de peito aos 42 dias de idade das aves. A biodisponibilidade média encontrada para a Lisina Sulfato foi de 100,19%, mostrando não haver diferença significativa na biodisponibilidade das lisinas testadas.

Consumo, digestibilidade dos nutrientes e desempenho de bovinos de corte recebendo silagem de sorgo (Sorghum bicolor (L.) Moench) e diferentes proporções de concentrado

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Sunflower meal for broilers of 22 to 42 days of age

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Tamanho da Partícula do Milho e Forma Física da Ração e Seus Efeitos Sobre o Desempenho e Rendimento de Carcaça de Frangos de Corte

Este experimento foi realizado com o objetivo de investigar os efeitos de diferentes granulometrias, expressa em Diâmetro Geométrico Médio (DGM) do milho (0,336mm, 0,585mm, 0,856 mm e 1,12 mm) de dietas fornecidas na forma farelada (FAR) e peletizada (PEL), no desempenho e no rendimento de carcaça e de cortes de frangos de corte de 21 a 42 dias de idade. Dietas FAR, produzidas com DGM de 0,336 mm resultaram em um menor consumo (p<0,001), ganho de peso (p<0,001) e pior conversão alimentar (p<0,001) do que as PEL de mesmo DGM. Os demais DGM não mostraram diferenças entre ração FAR e PEL. Quando avaliada somente a granulometria, observou-se que o aumento no DGM melhorou o ganho de peso linearmente e de forma quadrática o consumo e a conversão alimentar. Não houve influência da forma física ou DGM em rendimento de carcaça e rendimento de perna+coxa. Porém foi verificada uma redução em rendimento de peito com DGM 0,336 mm (p<0,001), na forma FAR.

Efeito do período de jejum na fase final de crição de frangos de corte machos sobre rendimento e composição da carcaça

The objective of this work was to evaluate the effect of fasting period in the last growing phase on carcass yield and composition of male broilers. Two thousand one-day old male chicks were distributed in five randomized blocks according to a 4x2 factorial (four feeding programs (P): ad libitum or one of three fasting schedules: 8-12, 12-16 and 8-16; and two strains (S): Ross or Hubbard-Peterson, Fifty birds were used per replicate. Birds were raised under identical feed and management conditions until day 42. The fasting schedules were applied from day 43 to day 56. At day 56, five birds per replicate were randomly sampled, weighed, slaughtered, eviscerated, dry-cooled, cut and deboned. No effects of P or SxP interaction were observed for carcass characteristics. birds, which showed higher weights and yields of head plus neck, feet, leg bones and wings. The ad libitum birds showed higher crude protein in thigh meat than those submitted to the 8-12 h fast. A SxP interaction was observed for meat ash content. The R broilers showed higher ash content in breast and thigh meat than the H birds in the 8-12 h fast treatment. on the other hand, the R broilers submitted to the 8-12 h fast showed higher ash contents in breast and thigh meat than birds from the same strain in the other feeding programs. Fasting in the last phase of rearing did not alter the yield of whole carcass, carcass cuts and abdominal fat, but morning fast influenced carcass chemical composition.

Uso do ácido orgânico (ácido fumárico) nas rações de frangos de corte

This experiment was carried out to evaluate the effect of the addition of fumaric acid to broiler diet on birds performance. One thousand and eighty Hubbard day-old broiler chicks were alloted in a randomized block design, six treatments with two replicates of males, and four replicates of females. Three basal diets were formulated to meet the nutritional requirements in each growing phase: from 1 to 21 days, 21 to 37 days and 37 to 45 days. Treatments consisted first in the addition of growth promoter to 0.75 and 1.0%, of fumaric acid. The group treated without of both growth promoter and fumaric acid presented a higher intake and a smaller feed:gain ratio in relation to the fumaric acid treated groups. However, there were no differences among groups treated with growth promoter and fumaric acid. The contrasts did not show any difference among weight gain, carcass yield and abdominal fat. The addition of fumaric acid levels to the diets promoted reduction of feed intake, without any effect on weight gain, improving, therefore, the feed/gain ratio. A digestibility experiment was carried out, using 30 Hy-Line roosters, to determine apparent metabolyzed energy (AME), corrected by nitrogen of the diets containing 0.0, 0.5 and 1.0% of the acid. An increase on the AME of the diets was observed with fumaric acid addition.

Dietary vitamin or mineral mix removal during the finisher period on broiler chicken performance

The effect of dietary vitamin (VS) and mineral (MS) mix withdrawal between 42 and 49 d of age on broiler chicken performance was evaluated. The diets were formulated based on corn and soybean meal, and the experiments were conducted in floor pens using wood shavings as litter. Trial I evaluated withdrawal of VS and MS mix at 42, 45, and 49 d. The results of this trial showed that VS and MS mix withdrawal at 42 d of age did not impair feed intake (FI) or weight gain (WG), but feed conversion (FC) was improved (P = 0.043) when both mixes were maintained in the diets until 49 d of age. Trial 2 assessed the withdrawal of VS or MS mix at 42 d of age. FI and WG were not affected by withdrawal, but FC was poorer (P = 0.035) for the broilers fed the diet without VS. The relative liver weight was reduced by VS or MS mix withdrawal (P = 0.014 and P = 0.001, respectively). Carcass, breast, and leg yields were not affected by VS or MS mix withdrawals in either trial. The findings of the study suggested that VS mix withdrawal during the final period of broiler chicken growth was more deleterious than the withdrawal of MS mix, because it affected FC ratio. VS or MS withdrawal during this period did not affect carcass yield.

Use of ideal protein concept for precision formulation of amino acid levels in fish-meal-free diets for juvenile Nile tilapia (Oreochromis niloticus L.)

This study was undertaken in a closed system with Nile tilapia (Oreochromis niloticus) to examine the effects of total replacement of fish meal (FM) by soybean meal. Nile tilapia fingerlings with an average weight of 5.34+/-0.08 g were hand-fed one of the five isoenergetic (approximate to13.5 MJ digestible energy kg(-1)) and isoproteic (approximate to31% of digestible protein) experimental diets to satiation, six times a day during 85 days in eight replicate fibreglass tanks (six fish per tank). The control diet containing FM was substituted by soybean meal, with and without essential amino acids (lysine, methionine and threonine) or dicalcium phosphate supplementation. The supplemental amino acids were added at levels to simulate the reference amino acid profile of Nile tilapia carcass protein, based on the ideal protein concept. The results showed that soybean meal diet supplemented only with dicalcium phosphate was inferior to the control diet with FM and soybean meal diets supplemented with dicalcium phosphate and essential amino acids. Multiple essential amino acids and dicalcium phosphate incorporation in soybean meal diets was associated with performance, whole-body composition and carcass yield equal to that of the fish fed with the control diet containing FM. These data suggest that a diet with all plant protein source, supplemented with essential amino acids, based on tissue amino acid profile, can totally replace FM in a diet for Nile tilapia, without adverse effects on the growth performance, carcass yield and composition.

Influence of Dietary Lysine Levels and Arginine:Lysine Ratios on Performance of Broilers Exposed to Heat or Cold Stress during the Period of Three to Six Weeks of Age

Four trials of identical experimental design were conducted to determine the effects of temperature, dietary Lys level, and dietary Arg:Lys ratios on performance and carcass yield of male broilers. Birds of a commercial strain were grown from 21 to 42 d of age in wire-floored finishing batteries placed in environmental chambers. The chambers were programmed to provide either a constant thermoneutral temperature (21.1 C), a constant cold temperature (15.5 C), or a cycling hot diurnal temperature (25.5 to 33.3 C). Within each environment there was a factorial arrangement of three Lys levels (1.0, 1.1, and 1.2%) with four Arg:Lys ratios (1.1:1, 1.2:1, 1.3:1, and 1.4:1). Environmental temperature significantly influenced virtually every characteristic examined. Hot cyclic temperatures reduced weight gain, feed intake, and breast meat yield, and increased feed conversion, dressing percentage, leg quarter yield, and abdominal fat content. The cold environment promoted increased feed intake and mortality. Ascites and cardiomyopathy were the leading causes of death under cold exposure and thermoneutral conditions, whereas complications arising from heat exposure were the main cause of death under hot cyclic conditions. Levels of Lys affected leg quarter yield and abdominal fat content over all environments but increased breast meat yield only under cold conditions. Increasing Arg: Lys ratios improved feed conversion and dressing percentage and reduced abdominal fat content; it could not be determined whether these responses were consistent with Arg per se or were due to a nonspecific N response. As increasing Lys levels or Arg:Lys ratios did not improve weight gain, increase breast meat yield, or attenuate adverse effects due to heat or cold exposure, it is concluded that the levels of Lys and Arg suggested for 21 to 42 d by the NRC are adequate for birds of this age under the environmental conditions encountered.

Efeitos da densidade populacional e da energia da dieta sobre o desempenho de frangos de corte

The experiment was conducted to determine the effect of feeding three levels of energy (2800, 3000 and 3200 kcal ME/kg) in diets and two stocking densities (10 and 22 birds/m 2) on broilers performance. The experimental design was randomized blocks in a 3 × 2 × 2 factorial arrangement with four replication per treatment. The factors studied were levels of energy in the diet, population density, and sex. The birds were killed at 42 days of age. The data indicate that increasing energy levels in the diets of broilers, 1 to 42 days of age, reduced feed intake, improved feed conversion, and increased the amount of abdominal fat, energy intake, and weight gain without affecting carcass yield. There was no significant effect of diet on the live weight production of broilers produced per area of floorspace or on mortality. The higher values for carcass yield were obtained for birds raised under the higher population density and for males in comparison to females. With the exception of the undesirable elevation in the amount of abdominal fat, the increase in the level of energy in the diets resulted, in general, in an improvement in the performance of the broilers independent of the population density. However, the response to the amount of energy in the diet was similar for both population densities.

Efeitos de diferentes densidades populacionais nas estações fria e quente do ano sobre o desempenho de frangos de corte

The objective of this study was to evaluate the performance of broilers reared under different population densities in the cold and hot seasons of the year. Two identical experiments were conducted, one during the winter for 49 days, and the other during the summer where the chicks were slaughtered at 42 days of age. Commercial Hubbard broiler-type chicks were distributed in a randomized block design in a 4 × 2 factorial arrangement with population densities of 10, 14, 18, and 22 birds/m 2, by sex, and with four replications. Feed intake was reduced with a progressive decrease of available space for the chicks, and it resulted in a linear decrease in weight gain. However, there was a linear increase in the live weight of broilers in kilograms per area of floor space, proportional to the increase of population density, without effect on the viability. The progressive increment in the population density reduced the observed feed/gain ratio during the total winter rearing period for both sexes. There was no effect of population density on this characteristic during the hot season of the year. Population density did not affect the carcass yield of chicks during the summer. However, there was a linear increase on carcass yield with an increase in population density in the winter. The males, at the age slaughtered, had higher weights, feed intakes, and better feed/gain ratios, and lower abdominal fat and higher live weight production in kilograms per floor area than females. However, the viable index for the males was lower. From the results obtained, there was a linear increase in the liveweight of broilers in kilograms per floor area, making it possible to rear broilers under higher population densities, independent of the season of the year, as well as the occurrence of a decrease in feed intake and weight gain of the chicks with a decrease of available space.

Different criteria for feed formulation based on digestible amino acids for broilers

An experiment was carried out to evaluate different criteria in feed formulation based on digestible amino acids for broilers during the grower phase. Diets were formulated according to the recommendations for digestible methionine, methionine + cysteine, lysine, and threonine. A total number of six hundred Cobb 500 male day-old chicks were distributed in a completely randomized experimental design, with three formulation criteria supplying the recommendations established by Baker & Chung (1992), Degussa (1997), and Rostagno et al. (2000), with 4 replicates of 50 birds each. No significant differences were observed for weight gain and feed intake. However, feed conversion ratio improved when birds were fed the diets containing the profiles recommended by Baker & Chung (1992) and Degussa (1997). No difference was observed in terms of leg, wings, back, and head yields. Broilers fed with the profile of Baker & Chung (1992) presented worst breast yield, whereas those fed the Degussa (1997) standard had better carcass yield.

Desempenho e morfometria duodenal de frangos de corte submetidos a diferentes níveis de energia e programas de alimentação de 42 a 57 dias de idade

This experiment was conducted to evaluate the use of different energy levels and amino acid recommendations on performance, carcass yield and intestinal morphometry of broilers from 42 to 57 days of age. We used 1,600 one-day old male broilers (Cobb 500) in a completely randomized design arranged in a 2x4 factorial scheme with 3,200 and 3,600 kcal ME/kg and four different feed programs. The metabolizable energy levels and the feed programs did not determine significant differences in carcass characteristics for choosing a level of energy or a feed program, thus the energy level and the recommendations of amino acids that determine the highest cost-benefit ratio should prevail. It was observed that 3,600 kcal ME/kg resulted in some improvement on performance and morphometry of the intestinal mucosa, also the fractioning of digestible amino acid requirements during two periods resulted in worse performance and intestinal villi height.

Differentially transcribed genes in skeletal muscle of lambs

The objective of this study was to compare gene transcription profiles in Longissimus dorsi muscle of the following four hair sheep genetic groups, Morada Nova (MO), Brazilian Somali (SO), Santa Inĉs (SI) and 1/2 Dorper×1/2 Morada Nova (F1). These groups all display different postnatal muscle growth. The transcriptomes of the skeletal muscle of the lambs (at 200 days of age) were profiled by using oligonucleotide microarrays and reverse transcription-quantitative real-time PCR (RT-qPCR). The microarray experiment identified 262 transcripts that were differentially expressed when transcription levels were compared between the different breeds. A total of 23 transcripts among which those involved in skeletal muscle development (MyoD1 and IGFBP4), lipogenesis and adipogenesis (C/EBPδ, PPARγ and PGDS) were differentially expressed in at least in one comparison. Clustering analysis showed that there is greater similarity in gene expression between the MO and SI breeds and between F1 and SO genetic groups. The SO breed has the most distinct expression pattern. The RT-qPCR results confirmed the findings from the microarray study. A positive correlation was observed between the expression of MyoD1 and the cold carcass yield. The negative correlations between the weight and yield of cold carcass with the expression of C/EBPδ mean that the selection for adipogenesis could lead to a lower carcass weight. The GLUT3 and PYGL gene transcripts were negatively correlated with fat thickness, but ATP5G1 was positively correlated with this trait. Interestingly, many genes negatively correlated with PUFA were positively correlated with cold carcass yield. In conclusion, the present work demonstrated that there are breed-specific expression patterns in Brazilian hair sheep genetic groups. The differences in gene expression among genetic groups were consistent with their phenotypic differences. The positive correlation of the MyoD1 expression with the cold carcass yield suggests that this gene is important for tissue growth in sheep. The positive correlation of the C/EBPδ expression with PUFA provides an opportunity to select for lipid deposition in meat animals. © 2012 Elsevier B.V.

Exigência de treonina digestível para codornas de corte no período de 15 a 35 dias de idade

The digestible threonine (DThr) requirements for meat quails (Coturnix coturnix sp) during growing phase from 15 to 35 days of age were estimated, using 1,020 quails with 15 days of age, of both sexes, distributed in a completely randomized design, with six levels of digestible threonine-0.93; 1.00; 1.07; 1.14; 1.21 and 1.28% in diet, five replications and 34 quails per experimental unit. There was no effect on performance variables, carcass yield and chemical composition and nitrogen balance with the studied increase in digestible threonine levels. There was a linear increase in threonine intake and feed cost per kilogram of body weight gain with increasing levels of digestible threonine. It was concluded that the best level of threonine evaluated was sufficient to meet the requirements of meat quails, in the period from 15 to 35.