The Non-catalytic ``Cap Domain'' of a Mycobacterial Metallophosphoesterase Regulates Its Expression and Localization in the Cell

Despite highly conserved core catalytic domains, members of the metallophosphoesterase (MPE) superfamily perform diverse and crucial functions ranging from nucleotide and nucleic acid metabolism to phospholipid hydrolysis. Unique structural elements outside of the catalytic core called ``cap domains'' are thought to provide specialization to these enzymes; however, no directed study has been performed to substantiate this. The cap domain of Rv0805, an MPE from Mycobacterium tuberculosis, is located C-terminal to its catalytic domain and is dispensable for the catalytic activity of this enzyme in vitro. We show here that this C-terminal extension (CTE) mediates in vivo localization of the protein to the cell membrane and cell wall as well as modulates expression levels of Rv0805 in mycobacteria. We also demonstrate that Rv0805 interacts with the cell wall of mycobacteria, possibly with the mycolyl-arabinogalactan-peptidoglycan complex, by virtue of its C terminus, a hitherto unknown property of this MPE. Using a panel of mutant proteins, we identify interactions between active site residues of Rv0805 and the CTE that determine its association with the cell wall. Finally, we show that Rv0805 and a truncated mutant devoid of the CTE produce different phenotypic effects when expressed in mycobacteria. Our study thus provides a detailed dissection of the functions of the cap domain of an MPE and suggests that the repertoire of cellular functions of MPEs cannot be understood without exploring the modulatory effects of these subdomains.

Stable isotope evidence for ca. 2.7-Ga-old Archean cap carbonates from the Dharwar Supergroup, southern India

Carbon isotope compositions of carbonate rocks from similar to 2.7-Ga-old Neoarchean Vanivilas Formation of the Dharwar Supergroup presented earlier by us are re-evaluated in this study, besides oxygen isotope compositions of a few silica dolomite pairs. The purpose of such a revisit assumes significance in view of recent field evidences that suggest a glaciomarine origin for the matrix-supported conglomerate member, the Talya conglomerate, which underlies the carbonate rocks of the Vanivilas Formation. An in-depth analysis of carbon isotope data reveals preservation of their pristine character despite the rocks having been subjected to metamorphism to different degrees (from lower greenschist to lower amphibolite facies). The dolomitic member of Vanivilas Formation of Marikanive area is characterized by highly depleted delta C-13 value (up to -5 parts per thousand VPDB) and merits as the Indian example of ca. 2.7-Ga-old cap carbonate. This inference is further supported by estimated low temperature of equilibration documented by a few silica dolomite pairs from the Vanivilas Formation collected near Kalche area. These pairs show evidence for oxygen isotopic equilibrium at low temperatures (similar to 0-20 degrees C) with depleted water (delta O-18 = -21 parts per thousand to -15 parts per thousand VSMOW) of glacial origin. We propose that the mineral pairs were deposited during the deglaciation period when the ocean temperature was in its gradual restoration phase. The dolomite of Marikanive area is the first record of cap carbonates from the Indian subcontinent with Neoarchean antiquity.

XCRI-CAP PG Course Implementation Manual

This an implementation manual for institutions wanting to implement XCRI-CAP for PG courses and send feeds to Prospects

Diário da Constituinte [gravação de vídeo] : [programa n. 659 1ª ed.]

O movimento Criança Constituinte quer garantir no novo texto o artigo que dá aos menores infratores o direito de defesa, este dispositivo tem sido combatido por alguns juristas que defendem a manutenção do atual Código de Menores. Os integrantes do movimento Criança Constituinte são favoráveis ao voto aos 16 anos, mas sem a redução da idade para a responsabilidade criminal. A tarde começará a primeira sessão de votação do 2º Turno da Constituinte. Nesta etapa as emendas só podem cortar parte do texto e corrigir erros ou contradições. Para o 2º Turno os constituintes apresentaram 1844 Emendas e 1744 foram destacadas, mas ao contrário do 1º Turno o Destaque não dá preferência na votação. Os dispositivos que os constituintes mais querem mudar no 2º Turno são os que tratam dos direitos dos trabalhadores, da anistia financeira, do voto aos 16 anos, da reforma agrária e do tabelamento de juros. Carlos Sant’Anna (Líder do Governo) acredita que para os temas polêmicos haverá quórum para decisões

Diário da Constituinte [gravação de vídeo] : [programa n. 659 2. ed.]

Constituinte se reúnem para decidir o que será votado no segundo turno de votação do texto constitucional. Falta quorum na primeira sessão de votação que se realizou naquela tarde. Líderes fecham acordo sobre os próximos itens a serem votados.

Istoria delle guerre del Regno del Brasile : accadute tra la corona di Portogallo e la Republica di Olanda, composta, ed offerta alla sagra reale maesda di Pietro Secondo, Re di Portogallo & C. dal P. F. Gio. Gioseppe di S. Teresa Carmelitano Scalzo : Parte prima e Parte seconda

As negociações entre Portugal e Holanda a respeito da devolução dos territórios portugueses ocupados se desenvolveram ao longo de um decênio. As propostas iniciais de Portugal referiam-se aos territórios ocupados pelos holandeses na África e no Maranhão. Estão no raríssimo folheto.

Interview with Claire Gibbons about implementing XCRI-CAP

Claire spoke to Janette Hillicks about the University of Bradford's implementation of XCRI-CAP.

Histoire de Portugal : contenant les enterprises, navigations, & gestes memorables des Portugallois, tant en la cõqueste des Indes Orientales par eux descouvertes, qu'és guerres d'Afrique & autres exploits, depuis l'an mil quatre cens nonãte six, jusques à l'an mil cinq cens septante huit, sous Emmanuel premier, Jean troisieme, & Sebastian premier du nom / Pour Antoine Chuppin, ed. ; comprinse en vingt livres, dont les douze premiers sont traduits du latin de Ierosme Osorius, Euesque de Sylues en Algarve, les huit suivans prins de Lopez de Castagnede & d'autres historiensNouvellement mise en françois, par S.G.S. avec un discours du fruit qu'on peut recueillir de la lecture de cette histoire, & ample indice des matieres principales y contenues

Contém os empreendimentos, as navegações e os gestos memoráveis dos portugueses, inclusive em suas colônias.

NOAA Coastal Change Analysis Program (C-CAP): Guidance for Regional Implementation

EXECUTIVE SUMMARY: The Coastal Change Analysis Programl (C-CAP) is developing a nationally standardized database on landcover and habitat change in the coastal regions of the United States. C-CAP is part of the Estuarine Habitat Program (EHP) of NOAA's Coastal Ocean Program (COP). C-CAP inventories coastal submersed habitats, wetland habitats, and adjacent uplands and monitors changes in these habitats on a one- to five-year cycle. This type of information and frequency of detection are required to improve scientific understanding of the linkages of coastal and submersed wetland habitats with adjacent uplands and with the distribution, abundance, and health of living marine resources. The monitoring cycle will vary according to the rate and magnitude of change in each geographic region. Satellite imagery (primarily Landsat Thematic Mapper), aerial photography, and field data are interpreted, classified, analyzed, and integrated with other digital data in a geographic information system (GIS). The resulting landcover change databases are disseminated in digital form for use by anyone wishing to conduct geographic analysis in the completed regions. C-CAP spatial information on coastal change will be input to EHP conceptual and predictive models to support coastal resource policy planning and analysis. CCAP products will include 1) spatially registered digital databases and images, 2) tabular summaries by state, county, and hydrologic unit, and 3) documentation. Aggregations to larger areas (representing habitats, wildlife refuges, or management districts) will be provided on a case-by-case basis. Ongoing C-CAP research will continue to explore techniques for remote determination of biomass, productivity, and functional status of wetlands and will evaluate new technologies (e.g. remote sensor systems, global positioning systems, image processing algorithms) as they become available. Selected hardcopy land-cover change maps will be produced at local (1:24,000) to regional scales (1:500,000) for distribution. Digital land-cover change data will be provided to users for the cost of reproduction. Much of the guidance contained in this document was developed through a series of professional workshops and interagency meetings that focused on a) coastal wetlands and uplands; b) coastal submersed habitat including aquatic beds; c) user needs; d) regional issues; e) classification schemes; f) change detection techniques; and g) data quality. Invited participants included technical and regional experts and representatives of key State and Federal organizations. Coastal habitat managers and researchers were given an opportunity for review and comment. This document summarizes C-CAP protocols and procedures that are to be used by scientists throughout the United States to develop consistent and reliable coastal change information for input to the C-CAP nationwide database. It also provides useful guidelines for contributors working on related projects. It is considered a working document subject to periodic review and revision.(PDF file contains 104 pages.)

Diário da Constituinte [gravação de vídeo] : [programa n. 680 2ª ed.]

Os constituintes já garantiram vários aspectos dos direitos individuais e coletivos. Já aprovaram 27 incisos, mas faltam 57. Os direitos já foram garantidos: habeas data, defesa do consumidor, racismo como crime inafiançável, mandado de segurança e injunção. Os constituintes pedem maior rigor aos faltantes. O presidente Ulysses solicita a presença em plenário para a votação do 2º turno da Assembléia Constituinte, que deverá votar 708 destaques.

Diário da Constituinte [gravação de vídeo] : [programa n. 676 1ª ed.]

Não houve quórum para votação dos Direitos Sociais, com apenas 234 parlamentares. Foram sugeridas propostas para ampliar a presença no plenário, tais como: chamar os suplentes e suspensos os ausentes sistemáticos e, ou não se pague ao constituinte que não aparecer. Foram sugeridas criações de Comissões que cuidem de grupos de emendas por assunto, para facilitar a votação, pois não haverá atrasos com as negociações. O PSDB reuniu líderes dos partidos para discutir os item mais polêmicos dos direitos sociais.