Three-stage transformation of chlorophyll transient fluorescence pattern under sustained dehydration and the discovery of critical water content in seaweeds

The chlorophyll fluorescence kinetics of marine red alga Grateloupia turutunt Yamada, green alga Ulva pertusa Kjellm and brown alga Laminaria japonica Aresch during natural sustained dehydration were monitored and investigated. The pulse amplified modulation (PAM) system was used to analyze the distinct fluorescence parameters during thallus dehydration. Results proved that the fluorescence kinetics of different seaweed all showed three patterns of transformation with sustained water loss. These were: 1) peak kinetic pattern (at the early stage of dehydration fluorescence enhanced and quenched subsequently, representing a normal physiological state). 2) plateau kinetic pattern (with sustained water loss fluorescence enhanced continuously but quenching became slower, finally reaching its maximum). 3) Platform kinetic pattern (fluorescence fell and the shape of kinetic curve was similar to plateau kinetic pattern). A critical water content (CWC) could be found and defined as the percentage of water content just prior to the fluorescence drop and to be a significant physiological index for evaluation of plant drought tolerance. Once thallus water content became lower than this value the normal peak pattern can not be recovered even through rehydration, indicating an irreversible damage to the thylakoid membrane. The CWC value corresponding to different marine species were varied and negatively correlated with their desiccation tolerance, for example. Laminaria japonica had the highest CWC value (around 90%) and the lowest dehydration tolerance of the three. In addition, a fluorescence "burst" was found only in red algae during rehydration. The different fluorescence parameters F-o, F-v and F-v, F-m were measured and compared during water loss. Both F-o and F-v increased in the first stage of dehydration but F-v/F-m. kept almost constant. So the immediate response of in vivo chlorophyll fluorescence to dehydration was an enhancement. Later with sustained dehydration F-o increased continuously while F-v decreased and tended to become smaller and smaller. The major changes in fluorescence (including fluorescence drop during dehydration and the burst during rehydration) were all attributed to the change in F-o instead of F-v This significance of F-o indicates that it is necessary to do more research on F-o as well as on its relationship with the state of thylakoid membrane.

Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata

Antioxidant activity (AA), total phenolic content, and reducing power of the crude extract, fractions, and subfractions derived from a red alga, Polysiphonia urceolata, were evaluated and determined. The antioxidative activity was measured using the alpha,alpha-diphenyl-beta-pierylhydrazyl (DPPH) radical scavenging assay and the P-carotene-linoleate assay systems, and compared with that of butylated hydroxytoluene (BHT), gallic acid (GA), and ascorbic acid (AscA). The results showed that the crude extract and the ethyl acetate-soluble fraction exhibited higher AA than BHT in the DPPH assay model, at all of four concentration levels tested (from 0.4 to 50 mu g/ml), while, in the beta-carotene-linoleate assay system, the crude extract and the ethyl acetate-soluble fraction exhibited similar or, in most cases, higher AA than GA and AscA at the same concentrations (from 10 to 200 mu g/ml). The ethyl acetate-soluble fraction was further fractionated into seven subfractions F1-F7 by silica gel vacuum liquid chromatography. F1 was found to be the most effective subfraction in both assay systems. The total phenolic content and reducing power were determined using the Folin-Ciocalteu and the potassium ferricyanide reduction methods, respectively. Statistical analysis indicated a significant association between the antioxidant potency and total phenolic content as well as between the antioxidant potency and reducing power. (c) 2005 Elsevier Ltd. All rights reserved.

地震反射界面形态与介质各向异性属性联立成像：地震反射波旅行时与偏振信息综合反演

It has been a difficult problem faced by seismologists for long time that how exactly to reconstruct the earth's geometric structure and distribution of physical attributes according to seismic wave's kinematical and dynamic characteristics, obtained in seismological observation. The jointing imaging of seismic reflector and anisotropy attributes in the earth interior is becoming the research hot spot. The limitation of shoot and observation system makes that the obtained seismic data are too scarce to exactly reconstruct the geological objects. It is popular that utilizing only seismic reflection traveltimes or polarizations information make inversion of the earth's velocity distribution by fixing seismic reflector configuration (vice versa), these will lead to the serious non-uniqueness reconstruction due to short of effective data, the non-uniqueness problem of reconstructing anisotropy attributes will be more serious than in isotropy media. Obviously it is not enough to restrict the media structure only by information of seismic reflection traveltimes or polarizations, which even sometimes will lead to distorted images and misinterpretation of subsurface structure. So we try to rebuild seismic reflection structure (geometry) and media anisotropic structure (physics) in the earth interior by jointing data of seismic wave kinematics and dynamics characteristics, we carry out the new experiment step by step, and the research mainly comprises of two parts: one is the reconstruction of P-wave vertical velocity and anisotropic structure(Thomsen parameter s and 8) in the transversely isotropic media with vertical symmetrical axis(VTI) by fixing geometrical structure, and the other is the simultaneous inversion of the reflector surface conformation and seismic anisotropic structure by jointing seismic reflection traveltimes and polarizations data. Simulated annealing method is used to the first research part, linear inversion based on BG theory and Simulated annealing are applied to the second one. All the research methods are checked by model experiments, then applied to the real data of the wide-angle seismic profile from Tunxi, Anhui Province, to Wenzhou, Zhejiang Province. The results are as following The inversion results based on jointing seismic PP-wave or PSV-wavereflection traveltimes and polarizations data are more close to real model than themodels based simply on one of the two data respectively. It is shown that the methodwe present here can effectively reconstruct the anisotropy attributes in the earth'sinterior when seismic reflector structure is fixed.The layer thickness, P-wave vertical velocity and Thomsen anisotropicparameters {s and 8) could be resolved simultaneously by jointing inversion ofseismic reflection traveltimes and polarizations with the linear inversion methodbased on BG theory.The image of the reflector structure, P-wave vertical velocity and theanisotropy parameters in the crust could be obtained from the wide-angle seismicprofile from Tunxi (in Anhui Province), to Wenzhou (in Zhejiang Province). Theresults reveal the difference of the reflector geometrical structure and physicalattributes in the crust between Yangtze block and Cathaysia block, and attempt tounderstand the characteristics of the crustal stress field in the areas.

A mosca das frutas e seu controle.

1985

Planejamento e instalação de uma cantina para elaboração de vinho tinto.

O que produzir. Estudo do ambiente. Escolha do local. Tamanho da cantina. Aspectos gerais da construção. Partes que compõem a cantina e etapas da vinificação. Recebimento da uva. Fermentação: recipientes de fermentação, maceração, remonstagens, descuba, prensagem, fermentação lenta. Fermentação malolática: temperatura, acidez, oxigênio, antissépticos, presença de borras. Estabilização do vinho: trasfegas e atestos, estabilização tartárica, filtração. Amadurecimento do vinho em barrica de carvalho. Engarrafamento. Envelhecimento do vinho na garrafa. Tratamento de efluentes vinícolas. Laboratório: determinação do babo, densidade, acidez total, teor alcoólico, acidez volátil, pH, dióxido de enxofre livre (SO2), dióxido de enxofre total, açúcar redutor, papel do ácido málico. Registro do estabelecimento enológico. Registro do vinho.

Elaboração de vinho moscatel espumante.

Aspectos gerais da produção do Asti espumante na Itália; Elaboração do vinho moscatel espumante; Principais cultivares de videira (Vitis vinifera) recomendadas para elaboração do vinho moscatel espumante; Garrafas para o vinho moscatel espumante; Rolha para o vinho moscatel espumante; Colocação da rolha e da gaiola; Vinho moscatel espumante e a legislação brasileira; Características analíticas do vinho moscatel espumante brasileiro; Avaliação sensorial do vinho moscatel espumante; Considerações gerais sobre a espuma; Participação organoléptica do dióxido de carbono (CO2); Informações básicas sobre o consumo do vinho moscatel espumante.

Etyczny profil "Familiaris consortio"

Artykuł koncentruje się na etycznym aspekcie „Familiaris Consortio”, zakładając równocześnie bogatą problematykę pastoralną. Pytanie etyczne, które pojawiało się wraz z ukazaniem się tego dokumentu wiązało się z nastrojami wahania i oczekiwania w wyniku ujawnienia się silnej opozycji wobec nauki zawartej w encyklice „Humanae Vitae” Pawła VI. Adhortacja nie podejmuje bezpośredniej polemiki z przeciwnikami nauki Kościoła, lecz przedstawia pozytywny wykład całościowej nauki na temat powołania małżeństwa i rodziny. Powołanie stanowi w tym dokumencie podstawową kategorię teologiczno-moralną, wyjaśniającą i uzasadniającą istotne wymogi moralne małżeństwa i rodziny i przenoszącą życie ludzkie na poziom świętości. Powołanie ma swe źródło w Boskim planie zbawienia i objawia się wraz z dokonaniem dzieła zbawienia w Jezusie Chrystusie. Tajemnica powołania uobecnia się w dziejach ludzkości jako ukryta a zarazem objawiona w misterium Chrystusa i Kościoła. Powołanie rzuca istotne światło na stworzenie mężczyzny i kobiety czyli stworzenie rodziny opartej na fundamencie małżeństwa. Odtąd prawda antropologiczna posiada wymiar sakramentalny. Powołanie przenikając wewnętrznie rzeczywistość stworzoną, umieszcza w samej głębi bytu „nie dające się stłumić wezwanie: rodzino, stań się tym, czym jesteś” (FC 17). Bóg Stwórca i Odkupiciel powierzył człowiekowi – mężczyźnie i kobiecie- zadanie budowania rodziny mocą tej samej Miłości, przez którą istnieje świat i człowiek, oraz tej samej Miłości, którą Objawił Syn Boży na Krzyżu, ustanawiając nowy porządek stworzenia. Odtąd prawda życia ludzkiego i prawda miłości musi być odczytywana wyłącznie w świetle tajemnicy Paschalnej. To wezwanie płynące z głębi sakramentu odzywa się mocą łaski w sercu małżeństwa, w sercu męża i żony, którzy są wezwani do miłowania się tą samą Miłością, która płynie z Misterium Krzyża. Ta koncepcja powołania, wypływająca z sakramentalnego charakteru chrześcijańskiego istnienia stanowi klucz do rozstrzygania szczegółowych pytań mogących się pojawić w kontekście życia małżeńskiego. Wizja przedstawiona przez „Familiaris Consortio” jest jednorodna i charyzmatyczna, jest teologiczna i antropologiczna zarazem, dotyczy bowiem człowieka jako człowieka, a nie tylko „pewnych jednostek” ludzkich uwikłanych w sidła małżeńskie. W „Familiaris Consortio” jest jedna norma małżeńska i jedna norma rodzicielska: to jest zawsze ta sama Miłość, która z Serca Boga poprzez sakrament przenika do serca małżonków. Dlatego miłość małżeńska jest miłością rodzicielską, czyli odpowiedzialną za świętość tajemnicy zrodzenia, to jest równocześnie za świętość Boga – Stwórcy i za godność człowieka powoływanego na świat. Zarówno miłość małżeńska jak miłość rodzicielska, nie mogą istnieć bez czystości małżeńskiej. Tu leży prawdopodobnie główna przyczyna powodująca opór wobec nauki „Humanae Vitae”. „Familiaris Consortio” odrzuca wszelką manipulację antykoncepcyjna, podkreślając sprzeczność postawy antykoncepcyjnej nie tylko z prawem Bożym, ale i z istotą człowieczeństwa. Zawiera się to w twierdzeniu, które głosi, że pomiędzy prawidłowym życiem małżeńskim a antykoncepcją zachodzi nie tylko różnica moralna, lecz także antropologiczna (FC 32). Jest to surowy osąd tej praktyki, ale prawdziwy: ci, którzy stosują antykoncepcję, staczają się na poziom poniżej-ludzki.

Rodzina podstawową instytucją i wspólnotą życia społecznego

Rodzina to wspólnota osób i instytucja społeczna. Jej podstawą jest związek małżeński mężczyzny i kobiety oparty na ich wzajemnej miłości i wolnym wyborze. To instytucja prawa naturalnego, którego twórcą jest sam Bóg. Małżeństwo i rodzina to głęboka wspólnota życia i miłości oraz komunia osób. Ta specyficzna wspólnota odpowiada wzajemnie za siebie, rodzi i wychowuje następne pokolenie, humanizując społeczeństwo. Rodzina jest dobrem społecznym, którego potrzebuje społeczeństwo.

Measurement of the differential cross section for the reaction gamman-->pi- p from deuterium.

We report a measurement of the differential cross section for the gamman-->pi- p process from the CLAS detector at Jefferson Laboratory in Hall B for photon energies between 1.0 and 3.5 GeV and pion center-of-mass (c.m.) angles (thetac.m.) between 50 degrees and 115 degrees. We confirm a previous indication of a broad enhancement around a c.m. energy ([sqrt]s) of 2.1 GeV at thetac.m.=90 degrees in the scaled differential cross section s7dsigma/dt and a rapid falloff in a center-of-mass energy region of about 400 MeV following the enhancement. Our data show an angular dependence of this enhancement as the suggested scaling region is approached for thetac.m. from 70 degrees to 105 degrees.

Safety and efficacy of rivastigmine in adolescents with Down syndrome: long-term follow-up.

Following the completion of a 20-week, open-label study of the safety and efficacy of liquid rivastigmine for adolescents with Down syndrome, 5 of the 10 adolescents in the clinical trial continued long-term rivastigmine therapy and 5 did not. After an average period of 38 months, all 10 subjects returned for a follow-up assessment to determine the safety and efficacy of long-term rivastigmine use. Rivastigmine was well tolerated and overall health appeared to be unaffected by long-term rivastigmine use. Performance change on cognitive and language measures administered at the termination of the open-label clinical trial was compared between the two groups. No between-group difference in median performance change across the long-term period was found, suggesting that the long-term use of rivastigmine does not improve cognitive and language performance. However, two subjects demonstrated remarkable improvement in adaptive function over the long-term period. Both subjects had received long-term rivastigmine therapy. The discussion addresses the challenge of assessing cognitive change in clinical trials using adolescents with Down syndrome as subjects and the use of group versus individual data to evaluate the relevance of medication effects.

The role of tectonic uplift, climate, and vegetation in the long-term terrestrial phosphorous cycle

Phosphorus (P) is a crucial element for life and therefore for maintaining ecosystem productivity. Its local availability to the terrestrial biosphere results from the interaction between climate, tectonic uplift, atmospheric transport, and biotic cycling. Here we present a mathematical model that describes the terrestrial P-cycle in a simple but comprehensive way. The resulting dynamical system can be solved analytically for steady-state conditions, allowing us to test the sensitivity of the P-availability to the key parameters and processes. Given constant inputs, we find that humid ecosystems exhibit lower P availability due to higher runoff and losses, and that tectonic uplift is a fundamental constraint. In particular, we find that in humid ecosystems the biotic cycling seem essential to maintain long-term P-availability. The time-dependent P dynamics for the Franz Josef and Hawaii chronosequences show how tectonic uplift is an important constraint on ecosystem productivity, while hydroclimatic conditions control the P-losses and speed towards steady-state. The model also helps describe how, with limited uplift and atmospheric input, as in the case of the Amazon Basin, ecosystems must rely on mechanisms that enhance P-availability and retention. Our novel model has a limited number of parameters and can be easily integrated into global climate models to provide a representation of the response of the terrestrial biosphere to global change. © 2010 Author(s).

Trends in anemia management in US hemodialysis patients 2004-2010.

BACKGROUND: There have been major changes in the management of anemia in US hemodialysis patients in recent years. We sought to determine the influence of clinical trial results, safety regulations, and changes in reimbursement policy on practice. METHODS: We examined indicators of anemia management among incident and prevalent hemodialysis patients from a medium-sized dialysis provider over three time periods: (1) 2004 to 2006 (2) 2007 to 2009, and (3) 2010. Trends across the three time periods were compared using generalized estimating equations. RESULTS: Prior to 2007, the median proportion of patients with monthly hemoglobin >12 g/dL for patients on dialysis 0 to 3, 4 to 6 and 7 to 18 months, respectively, was 42%, 55% and 46% declined to 41%, 54%, and 40% after 2007, and declined more sharply in 2010 to 34%, 41%, and 30%. Median weekly Epoeitin alpha doses over the same periods were 18,000, 12,400, and 9,100 units before 2007; remained relatively unchanged from 2007 to 2009; and decreased sharply in the patients 3-6 and 6-18 months on dialysis to 10,200 and 7,800 units, respectively in 2010. Iron doses, serum ferritin, and transferrin saturation levels increased over time with more pronounced increases in 2010. CONCLUSION: Modest changes in anemia management occurred between 2007 and 2009, followed by more dramatic changes in 2010. Studies are needed to examine the effects of declining erythropoietin use and hemoglobin levels and increasing intravenous iron use on quality of life, transplantation rates, infection rates and survival.