MicroRNAs may mediate the down-regulation of neurokinin-1 receptor in chronic bladder pain syndrome

Bladder pain syndrome (BPS) is a clinical syndrome of pelvic pain and urinary urgency-frequency in the absence of a specific cause. Investigating the expression levels of genes involved in the regulation of epithelial permeability, bladder contractility, and inflammation, we show that neurokinin (NK)1 and NK2 tachykinin receptors were significantly down-regulated in BPS patients. Tight junction proteins zona occludens-1, junctional adherins molecule -1, and occludin were similarly down-regulated, implicating increased urothelial permeability, whereas bradykinin B(1) receptor, cannabinoid receptor CB1 and muscarinic receptors M3-M5 were up-regulated. Using cell-based models, we show that prolonged exposure of NK1R to substance P caused a decrease of NK1R mRNA levels and a concomitant increase of regulatory micro(mi)RNAs miR-449b and miR-500. In the biopsies of BPS patients, the same miRNAs were significantly increased, suggesting that BPS promotes an attenuation of NK1R synthesis via activation of specific miRNAs. We confirm this hypothesis by identifying 31 differentially expressed miRNAs in BPS patients and demonstrate a direct correlation between miR-449b, miR-500, miR-328, and miR-320 and a down-regulation of NK1R mRNA and/or protein levels. Our findings further the knowledge of the molecular mechanisms of BPS, and have relevance for other clinical conditions involving the NK1 receptor.

Die Geschichten der heiligen Schriften mit vielen Nutzanwendungen für die jisraelitische Jugend jedes Alters in Schule und Haus : mit 1 Zeittaf. u. 1 Charte d. heiligen Landes

von J. H. Hirsch

Jüdische Geschichte von der Zerstörung des 1. Tempels bis zur Gegenwart in Charakterbildern dargest.

von S. Müller

Jüdische Geschichte von der Zerstörung des 1. Tempels bis zur Gegenwart in Charakterbildern dargest.

von S. Müller

Aufrichtig und unpartheyische Prüfung des Dreyfachen Beweißes 1. Daß die Promotion e. Juden zum Docter Medicinae gegen die Christliche Religion ... seye, 2. Daß die jüdische Medici sehr schädl., u. unter Christen nicht zu dulden seyn, 3. Daß ein Christen Patient ... in e. jüdischen Medici Cur sich nicht begeben könne

dargethan von Johann Helfrich Sagittario u. untersucht durch Johann Friderich Rübel

Mus Hs 771 - No 1.: während der Reise : im Auftrag S:. Ehrw:. M: v.: St. componirte ich diese zwei Lieder zum 2ten Grad um, und [unleserlich] führte selbige den 27ten August 5830 [!], zum erstenmale aus. in [Stein als ein Symbol für Freimaurerei] Socrates

[Carl Oestreich]

Hilkot 'eglah 'arufah : sive de veterum Hebraeorum circa vitulam decollandam ritibus, ex Deut. XXI. v. 1. seqq.

Leipzig, Univ., Diss., 1696

Der lexikalische Ertrag der Konjekturalkritik von Jesaja Kap. 1-12

Bern, Phil. Diss., 1916

Measurement of flow harmonics with multi-particle cumulants in Pb+Pb collisions at √sNN=2.76 TeV with the ATLAS detector

ATLAS measurements of the azimuthal anisotropy in lead–lead collisions at √sNN = 2.76 TeV are shown using a dataset of approximately 7μb−1 collected at the LHC in 2010. The measurements are performed for charged particles with transversemomenta 0.5 < pT < 20 GeV and in the pseudorapidity range |η| < 2.5. The anisotropy is characterized by the Fourier coefficients, vn, of the charged-particle azimuthal angle distribution for n = 2–4. The Fourier coefficients are evaluated using multi-particle cumulants calculated with the generating function method. Results on the transverse momentum, pseudorapidity and centrality dependence of the vn coefficients are presented. The elliptic flow, v2, is obtained from the two-, four-, six- and eight-particle cumulants while higher-order coefficients, v3 and v4, are determined with two- and four-particle cumulants. Flow harmonics vn measured with four-particle cumulants are significantly reduced compared to the measurement involving two-particle cumulants. A comparison to vn measurements obtained using different analysis methods and previously reported by the LHC experiments is also shown. Results of measurements of flow fluctuations evaluated with multiparticle cumulants are shown as a function of transverse momentum and the collision centrality. Models of the initial spatial geometry and its fluctuations fail to describe the flow fluctuations measurements.

Search for pair-produced third-generation squarks decaying via charm quarks or in compressed supersymmetric scenarios in pp collisions at √s=8 TeV with the ATLAS detector

Results of a search for supersymmetry via direct production of third-generation squarks are reported, using 20.3  fb −1 of proton-proton collision data at √s =8  TeV recorded by the ATLAS experiment at the LHC in 2012. Two different analysis strategies based on monojetlike and c -tagged event selections are carried out to optimize the sensitivity for direct top squark-pair production in the decay channel to a charm quark and the lightest neutralino (t 1 →c+χ ˜ 0 1 ) across the top squark–neutralino mass parameter space. No excess above the Standard Model background expectation is observed. The results are interpreted in the context of direct pair production of top squarks and presented in terms of exclusion limits in the m ˜t 1, m ˜ X0 1 ) parameter space. A top squark of mass up to about 240 GeV is excluded at 95% confidence level for arbitrary neutralino masses, within the kinematic boundaries. Top squark masses up to 270 GeV are excluded for a neutralino mass of 200 GeV. In a scenario where the top squark and the lightest neutralino are nearly degenerate in mass, top squark masses up to 260 GeV are excluded. The results from the monojetlike analysis are also interpreted in terms of compressed scenarios for top squark-pair production in the decay channel t ˜ 1 →b+ff ′ +χ ˜ 0 1 and sbottom pair production with b ˜ 1 →b+χ ˜ 0 1 , leading to a similar exclusion for nearly mass-degenerate third-generation squarks and the lightest neutralino. The results in this paper significantly extend previous results at colliders.

Measurement of the centrality and pseudorapidity dependence of the integrated elliptic flow in lead-lead collisions at √sNN=2.76 TeV with the ATLAS detector

The integrated elliptic flow of charged particles produced in Pb+Pb collisions at √sNN = 2.76 TeV has been measured with the ATLAS detector using data collected at the Large Hadron Collider. The anisotropy parameter, v2, was measured in the pseudorapidity range |η| ≤ 2.5 with the event-plane method. In order to include tracks with very low transverse momentum pT, thus reducing the uncertainty in v2 integrated over pT, a 1 μb−1 data sample recorded without a magnetic field in the tracking detectors is used. The centrality dependence of the integrated v2 is compared to other measurements obtained with higher pT thresholds. The integrated elliptic flow is weakly decreasing with |η|. The integrated v2 transformed to the rest frame of one of the colliding nuclei is compared to the lower-energy RHIC data.

The differential production cross section of the phi (1020) meson in sqrts = 7 TeV pp collisions measured with the ATLAS detector

Ameasurement is presented of the φ×BR(φ → K+K−) production cross section at √s = 7 TeV using pp collision data corresponding to an integrated luminosity of 383 μb−1, collected with theATLAS experiment at the LHC. Selection of φ(1020) mesons is based on the identification of charged kaons by their energy loss in the pixel detector. The differential cross section ismeasured as a function of the transverse momentum, pT,φ , and rapidity, yφ, of the φ(1020) meson in the fiducial region 500< pT,φ <1200MeV, |yφ| < 0.8, kaon pT,K > 230 MeV and kaon momentum pK < 800 MeV. The integrated φ(1020)-meson production cross section in this fiducial range is measured to be σφ×BR(φ → K+K−) = 570 ± 8 (stat) ± 66 (syst) ± 20 (lumi) μb.

Libanon : exegetisch-homiletischer Kommentar zu den Psalmen ; Erster Teil: Buch 1. und 2.

von Josef Nobel