Qualidade pós-colheita de abacates "hass" mantidos em temperatura ambiente e tratados com diferentes radiações

This study aimed the avocado ‘Hass’ conservation with the use of radiation. We performed two experiments: Experiment I – fruits irradiated with different doses of cobalt-60 gamma irradiation(0,0; 0,2; 0,4; 0,6 e 1,0 kGy); Experiment II – fruit irradiated by electron accelerator in different doses (0,0; 0,48; 0,8; 1,12 e 1,45 MeV), both maintained at room temperature of 21±1 °C and at relative humidity of 79±5 %. Antioxidant capacity, total phenolic compounds, fresh weight loss, and respiration rate evaluation were performed every three days for 12 days. The experimental design was completely randomized with three replicates per treatment. For statistical analysis, the Tukey test at 5% probability was employed. Under the conditions in which the experiments were performed, the results showed that the gamma radiation use retained the fruits for 12 days, regardless the doses applied. The radiation by electron accelerator use also promote the fruits preservation, regardless the doses employed.

Estabilidade físico-química de um produto de abacate acondicionado em diferentes embalagens e conservado pelo frio

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação de resíduos orgânicos para o manejo de Fusariose em diferentes patossistemas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Imiquimod based transcutaneous immunization – insights and novel concepts

This work aims at developing a transcutaneous immunization (TCI) approach in order to activate cytotoxic T-cells. A tumor specific immune response was therefore generated by the TLR7-Agonist imiquimod. Five commercially available creams including the innovators product Aldara® 5% creme were assessed to ascertain their capability to induce an immune response in C57BL/6 mice after dermal administration. Moreover, creams were investigated regarding their imiquimod permeation in a Franz-diffusion cell model. Results obtained from this study were used to develop novel formulation approaches based on dissolved state imiquimod in a submicron scale range. High pressure homogenization ensured emulsification as well as particle size reduction. A freeze dried spreadable solid nanoemulsion based on sucrose fatty acid esters and oil components represented a major formulation approach. Within the scope of this approach the influence of pharmaceutical oils i.e. middle chain triglycerides, avocado oil, jojoba wax, and squalen was assessed towards their TCI performance. Furthermore, an aqueous jojoba wax based emulsion gel was developed. Unlike the innovators product, all formulations demonstrated a distinctly reduced imiquimod permeation across murine skin, a fact particularly evident in case of jojoba wax. Squalen significantly augmented in vivo immune response (p≤0.05 Mann-Whitney-Test). The emulsion gel demonstrated a 10fold decrease of imiquimod permeation. In comparison with the innovators product, the emulsion gel induced an equal immune response with a simultaneously enhanced tumor rejection in a mouse model.

Fruit quality sensing:Post-harvest ripeness

Firmness sensing of selected varieties of apples, pears and avocado fruits has been developed using a nondestructive impact technique. In addition to firmness measurements, postharvest ripeness of apples and pears was monitored by spectrophotometric reflectance measurements, and that of avocadoes by Hunter colour measurements. The data obtained from firmness sensing were analyzed by three analytical procedures: principal component, correlation and regression, and stepwise discriminant analysis. A new software was developed to control the impact test, analyse the data, and sort the fruit into specified classes, based on the criteria obtained from a training procedure. Similar procedures were used to analyse the reflectance and colour data. Both sensing systems were able to classify fruits w i th good accuracy.

Chrysanthemum chlorotic mottle viroid: Unusual structural properties of a subgroup of self-cleaving viroids with hammerhead ribozymes

The causal agent of chrysanthemum chlorotic mottle (CChM) disease has been identified, cloned, and sequenced. It is a viroid RNA (CChMVd) of 398–399 nucleotides. In vitro transcripts with the complete CChMVd sequence were infectious and induced the typical symptoms of the CChM disease. CChMVd can form hammerhead structures in both polarity strands. Plus and minus monomeric CChMVd RNAs self-cleaved during in vitro transcription and after purification as predicted by these structures, which are stable and most probably act as single hammerhead structures as in peach latent mosaic viroid (PLMVd), but not in avocado sunblotch viroid (ASBVd). Moreover, the plus CChMVd hammerhead structure also appears to be active in vivo, because the 5′ terminus of the linear plus CChMVd RNA isolated from infected tissue is that predicted by the corresponding hammerhead ribozyme. Both hammerhead structures of CChMVd display some peculiarities: the plus self-cleaving domain has an unpaired A after the conserved A9 residue, and the minus one has an unusually long helix II. The most stable secondary structure predicted for CChMVd is a branched conformation that does not fulfill the rod-like or quasi-rod-like model proposed for the in vitro structure of most viroids with the exception of PLMVd, whose proposed secondary structure of lowest free energy also is branched. The unusual conformation of CChMVd and PLMVd is supported by their insolubility in 2 M LiCl, in contrast to ASBVd and a series of representative non-self-cleaving viroids that are soluble under the same high salt conditions. These results support the classification of self-cleaving viroids into two subgroups, one formed by ASBVd and the other one by PLMVd and CChMVd.

Ferulate-5-hydroxylase from Arabidopsis thaliana defines a new family of cytochrome P450-dependent monooxygenases.

The fah1 mutant of Arabidopsis is defective in the accumulation of sinapic acid-derived metabolites, including the guaiacyl-syringyl lignin typical of angiosperms. Earlier results indicated that the FAH1 locus encodes ferulate-5-hydroxylase (F5H), a cytochrome P450-dependent monooxygenase (P450) of the general phenylpropanoid pathway. We have cloned the gene encoding this P450 by T-DNA tagging and have confirmed the identity of the cloned gene by complementation of the mutant phenotype. F5H shows 34% amino acid sequence identity with the avocado ripening-induced P450 CYP71A1 and 32% identity with the flavonoid-3',5'-hydroxylases of Petunia hybrida. In contrast, it shares much less homology with cinnamate-4-hydroxylase, a P450 that catalyzes the hydroxylation of cinnamic acid three steps earlier in the general phenylpropanoid pathway. Since the highest degree of identity between F5H and previously sequenced P450s is only 34%, F5H identifies a new P450 subfamily that has been designated CYP84.

The Power of the Placement of Public Sculpture

The Power of the Placement of Public Sculpture explores the aesthetic effects public sculpture has on the environment around it. The work presented includes a discussion of case studies in select American and international communities. Relevance is brought to the topic through the documentation of the placement of a fabricated eight-foot avocado sculpture displayed on the University of Denver campus. Reflection on the experience exposes additional questions and demonstrates the importance of the placement of a public sculpture.

Seasonal changes in Florida avocados /

Literature cited: p. 27-28.

A novel plant toxin, persin, with in vivo activity in the mammary gland, induces Bim-dependent apoptosis in human breast cancer cells

Phytochemicals have provided an abundant and effective source of therapeutics for the treatment of cancer. Here we describe the characterization of a novel plant toxin, persin, with in vivo activity in the mammary gland and a p53-, estrogen receptor-, and Bcl-2-independent mode of action. Persin was previously identified from avocado leaves as the toxic principle responsible for mammary gland-specific necrosis and apoptosis in lactating livestock. Here we used a lactating mouse model to confirm that persin has a similar cytotoxicity for the lactating mammary epithelium. Further in vitro studies in a panel of human breast cancer cell lines show that persin selectively induces a G(2)-M cell cycle arrest and caspase-dependent apoptosis in sensitive cells. The latter is dependent on expression of the BH3-only protein Bim. Bim is a sensor of cytoskeletal integrity, and there is evidence that unique structure of the compound, persin could represent a novel class of microtubule-targeting agent with potential specificity for breast cancers.

Between-tree variation in fruit quality and fruit mineral concentrations of Hass avocados

Inconsistent internal fruit quality in Hass avocados affects consumer confidence. To determine the influence of individual trees on fruit quality, Hass avocado fruit were harvested from adjacent trees of similar external appearance in 3 commercial orchards in 1998 and 1 orchard in 1999. The trees in each orchard were grown with similar commercial practices and in similar soil types. Within each location, there were significant (P < 0.05) differences in the mean ripe fruit quality between trees with respect to fruit body rot severity ( mainly anthracnose) with and without cold storage, internal disorders severity due to diffuse discolouration and vascular browning ( after cold storage), days to ripen, percentage dry matter, and the percentage of the skin area with purple-black colour when ripe. These effects were also noted in the same orchard in 1999. There were significant (P < 0.05) differences in fruit flesh calcium, magnesium, potassium, boron and zinc concentrations between trees. Significant (P < 0.05) correlations were observed between average fruit mineral concentrations in each tree ( particularly calcium, magnesium and potassium) and body rot severity, percentage dry matter and fruit mass. There was little conclusive evidence that characteristics such as the growth of the non-suberised roots or the degree of scion under- or overgrowth was involved in these tree effects; however, differences between trees with respect to other rootstock characteristics may be involved. The inconsistency of the correlations across sites and years suggested that other factors apart from tree influences could also affect the relationship between fruit minerals and fruit quality.

Farmers’ Market Nutrition Program Recipe Cards

These are cards giving recipes for fruit and vegetable dishes. Recipes include: confetti bean salsa, Mediterranean quinoa salad, minty watermelon cucumber salad, quick refrigerator pickles, broccoli salad, apple, cranberry and almond coleslaw, farmers’ market pasta salad, roasted beet and butter bean hummus, spinach and strawberry pecan salad, okra, avocado and tomato salad, plum-berry mint smoothie and crispy baked vegetables.

Desenvolvimento e crescimento da espécie de cogumelo Pleurotus ostreatus em garrafas de plástico reutilizado

Dissertação de Mestrado, Tecnologia e Segurança Alimentar, 22 de Junho de 2016, Universidade dos Açores.

ADAPTABILIDAD DE LAS FAMILIAS AGRÍCOLAS ANTE PRESIONES ECONÓMICAS Y ECOLÓGICAS: UN CASO DE CAFÉ DE ALTURA EN LEÓN CORTÉS, COSTA RICA

Resumen Los medios de vida de las familias productoras de café de altura están ligados a la finca. Esta relación tan marcada (familia – finca) los hace vulnerables a presiones externas que alteran el funcionamiento cotidiano interno de la unidad productiva. Dentro de estas presiones externas se encuentran los bajos precios, efectos climáticos y plagas en el cafetal. El artículo busca identificar los impactos que ejercen dichas presiones sobre los medios de vida de las familias cafetaleras del cantón de León Cortés, además se intenta mostrar las estrategias que han seguido estas unidades familiares para poder soportar y reponerse a los impactos generados por el clima, las plagas y la inestabilidad de precios del café. Las familias han generado diversas “estrategias de adaptación” en relación con los precios bajos, las organizaciones de productores han buscado nuevas alternativas de comercialización del café, tales como mercado justo y denominación de origen; además, han buscado la generación de un mayor valor agregado mediante la diversificación del producto final enfocado al café de calidad y a la presentación de café molido. Por su parte, las unidades familiares han buscado alternativas de diversificación como la siembra de aguacate. Para combatir las plagas los productores se han enfocado en la introducción de prácticas de conservación de suelos, mejorar sistemas de recolección e introducir sombra en los cultivos; el combate químico también ha sido una estrategia. Por último, la presión del clima no había sido muy tomada en cuenta por los caficultores de la zona, sin embargo, la Tormenta Alma sufrida en el año 2008 hizo que los caficultores comenzaran a pensar en un manejo adecuado y sostenible de la finca que minimice el riesgo climático. Abstract Livelihoods of family coffee growers are strongly determined by their farm. This makes families vulnerable to external shocks that affect the operations in the plot. Within these external shocks are included low prices, pests, and climate effects on the plantation. This paper aims to identify the impact of the aforementioned shocks on family coffee grower livelihoods in the canton of León Cortés. For this purpose, the article shows the strategies followed by families in order to support and recover themselves from the impact which come from climate, pests, and coffee price instability. Families have pursued various “adapting strategies”. Regarding low prices, producers' organizations have sought coffee marketing alternatives, such as fair trade and appellation of origin. Likewise, they work on increasing added value through diversification, improving coffee quality, and by a better packaging of ground coffee. Also, households have sought diversification options, such as avocado production. In order to combat pests, producers have focused on the introduction of soil conservation practices, improvement of collection systems, growing trees on coffee plantations, and chemical control. Finally, climate shocks had not been considered as a real problem by the farmers until the storm Alma affected the region. Nowadays, they think more about sound and sustainable management for their farm.

Farmers’ Market Nutrition Program Recipe Cards

These are cards giving recipes for fruit and vegetable dishes. Recipes include: confetti bean salsa, Mediterranean quinoa salad, minty watermelon cucumber salad, quick refrigerator pickles, broccoli salad, apple, cranberry and almond coleslaw, farmers’ market pasta salad, roasted beet and butter bean hummus, spinach and strawberry pecan salad, okra, avocado and tomato salad, plum-berry mint smoothie and crispy baked vegetables.