一种羧甲基海藻糖的吸湿保湿剂及其制备

本发明涉及日化领域及医药行业，具体讲是一种羧甲基海藻糖的吸湿保湿剂及其制备。羧甲基海藻糖的吸湿保湿剂，采用羧甲基海藻糖为吸湿保湿剂。制备方法：所述吸湿保湿剂的羧甲基海藻糖的由按摩尔质量比为1∶ 4-6的经碱性异丙醇溶液溶胀的海藻糖与氯乙酸在过量的碱性异丙醇溶液中以30℃-60℃条件下反应2-5h，而后抽滤、烘干即得。本发明通过有效的合成手段得到的羧甲基海藻糖，生产成本提高甚微，吸湿保湿性能提高明显。作为一种高效的吸湿保湿因子，本发明得到的羧甲基海藻糖有望成为解决透明质酸缺乏的替代品用于化妆品中。

Status and prospects of mackerel and tuna fishery in Bangladesh

Present status and future prospects of mackerel and tuna fisheries in Bangladesh were assessed during July 2003-June 2004. The work concentrated on the fishing gears, length of fishes, total landings and market price of the catch and highlighted the prospects of the fishery in Bangladesh. Four commercially important species of mackerels and tuna viz. Scomberomorus guttatus, Scomberomorus commerson, Rastrelliger kanagurta, and Euthynnus affinis were included in the study. About 95% of mackerels and tuna were caught by drift gill nets and the rest were caught by long lines (4%) and marine set-bag-net (1%). Average monthly total landing of mackerels and tunas was about 264 t, of which 147 t landed in Cox's Bazar and 117 t in Chittagong sites. Total catches of the four species in Cox's Bazar and Chittagong sites were found to be 956 and 762 t, respectively. The poor landing was observed during January-February and the peak landing was in November and July. Gross market value of the annual landing of mackerels and tunas (1,718 t) was found to be 1,392 latch taka. Nevertheless, the mackerel and tuna fisheries in Bangladesh are increasingly contributing to the marine fish production of the country and have very good potential for further expansion for both domestic and export market.

尖吻蝮蛇蛇毒出血毒素DaHT-3的纯化与性质

通过Sephadex G-75,DEAE-Sephadex A-50,Sephadex G-200和两次PBE聚焦层析,从尖吻蝮蛇(Dienagkistrodon acutus)蛇毒中纯化到一个分子量为56 000的出血毒素(DaHT-3),经氨基酸组成测定计算,由487个氨基酸残基组成。此成分在SDS-PAGE上显示出一条均一的蛋白染色带,用等电聚焦电泳测定,其pI为5.50。该出血成分的最小出血剂量是2.6#mu#g,具有蛋白水解酶活力,其活力为3.68,但没有精氨酸酯酶和磷脂酶A_(2)活力。用红外光谱仪研究DaHT-3在溶液中酰胺Ⅰ带的吸收谱,该毒素含有31.8%的#alpha#螺旋、56.1%的#beta#折叠和12.1%的转角;当加入EDTA螯合剂去除金属离子后,它们的#alpha#螺旋、#beta#折叠、转角和无规卷曲分别变为11%、26.4%、46.2%和16.5%,而出血活力和蛋白水解酶活力均丧失,表明该出血毒素是金属蛋白酶。

栖热菌属高温菌RH99-02菌株产类胡萝卜素的研究

从腾冲热海分离到最高生长温度达80℃的一株嗜热非芽孢菌RH99-02菌株，经鉴定属Thermus属，对其所产类胡萝卜素进行了吸收光谱扫描及薄层层析分析。发酵研究表明振荡培养时的菌体生物量和色素产量上均高于静置培养，培养至50h菌体生物量和色素产量达到最大值，菌体色素产量为762μg/g干重。

鳜肿瘤坏死因子相关的凋亡诱导配体Trail基因的原核表达

中国科学院“百人计划”项目([2005]192)资助

马氏珠母贝(Pinctada matensii)3个DM结构域的克隆及序列分析

Dmr(tdouble sex and Mab-3 related transcription factor)是新发现的与动物性别决定和分化相关的基因家族(Kondo et al.,2002),在多种进化地位的物种中都具有高度保守性。然而在作为动物界第二大门的贝类中,是否也存在高度保守的Dmrt基因家族,是否具有丰富的多样性?到目前为止未见任何相关报道。马氏珠母贝是我国人工培育海水珍珠的最主要母贝,在其养殖群体中有少数雌雄同体个体,并在一定条件下出现性转化。因此,克隆鉴定马氏珠母贝的Dmrt基因既可丰富D

小麦交联淀粉的制备工艺研究

采用环氧氯丙烷为交联剂 ,以小麦淀粉为原料制备小麦交联淀粉。探讨了环氧氯丙烷用量 ,氢氧化钠用量 ,反应温度 ,反应时间对交联度的影响并利用design -expert 6 .0 .3软件响应面中心组合实验优化得出制备不同交联淀粉的最佳工艺条件 ,分析了双因素间的交互效应。

武汉东湖生态系中浮游物的营养结构

本工作主要探讨东湖水柱浮游物的颗粒有机物在营养结构上量的关系。定量测定武汉东湖浮游物的干重、无灰重及浮游动、植物生物量并进行相应的碳、氮、磷分析。1983和1984两年东湖浮游物总颗粒有机碳现存量(mg/m~3)分别为3993与2819(Ⅰ站),2958与1856(Ⅱ站);总颗粒有机氮为762与584(I站),565与369(Ⅱ站);总颗粒有机磷为105与56(Ⅰ站),59与37(Ⅱ站)。颗粒有机碎屑碳一般占浮游物总颗粒有机碳的50％,占浮游生物活体碳的61％—104％。与浮游植物生物量碳相比,有机碎屑碳

Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish

Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish.

Gene cloning and functional analysis of glycosaminoglycan-degrading enzyme chondroitin AC lyase from Flavobacterium columnare G(4)

The chondroitin AC lyase gene, cslA, was cloned for the first time from the fish bacterial pathogen F. columnare G(4). From the first transcription initiation site, the cslA extends 2620 nucleotides to the end of the 3' region. The open reading frame of cslA transcript has 2286 nucleotides encoding 762 amino acids with a 16 residues long signal peptide at the N-terminus. The gene, cslA was then successfully expressed in Escherichia coli and recombinant chondroitin AC lyase, rChonAC was purified, with its lytic activity analyzed. Zymography analysis copolymerized with chondroitin sulphate revealed the lytic activity of rChonAC and also the crude native ChonAC isolated from periplamic space of cultured F. columnare G(4). The low level of lytic activity observed in crude native ChonAC may be due possibly to the low level of expression of this gene in the cultured condition. The expression and the role of this virulence factor is of interest for further research on the pathogenesis of F. columnare.