Effect of dimethylsulfoxide on sphingomyelinase activity and cholesterol metabolism in Niemann-Pick type C fibroblasts

Niemann-Pick type C (NPC) fibroblasts present a large concentration of cholesterol in their cytoplasm due to a still unidentified deficiency in cholesterol metabolism. The influence of dimethylsulfoxide (DMSO) on the amount of intracellular cholesterol was measured in 8 cultures of normal fibroblasts and in 7 fibroblast cultures from NPC patients. DMSO was added to the fibroblast cultures at three different concentrations (1, 2 and 4%, v/v) and the cultures were incubated for 24 h. Sphingomyelinase activity was significantly increased in both groups of cells only when incubated with 2% DMSO (59.4 ± 9.1 and 77.0 ± 9.1 nmol h-1 mg protein-1, controls without and with 2% DMSO, respectively; 47.7 ± 5.2 and 55.8 ± 4.1 nmol h-1 mg protein-1, NPC without and with 2% DMSO, respectively). However, none of the DMSO concentrations used altered the amount of cholesterol in the cytoplasm of NPC cells (0.704 ± 0.049, 0.659 ± 0.041, 0.688 ± 0.063 and 0.733 ± 0.088 mg/mg protein, without DMSO, 1% DMSO, 2% DMSO and 4% DMSO, respectively). This finding suggests that sphingomyelinase deficiency is a secondary defect in NPC and shows that DMSO failed to remove the stored cholesterol. These data do not support the use of DMSO in the treatment of NPC patients.

Defining the keyhole modes – the effects on the weld geometry and the molten pool behaviour in high power laser welding of stainless steels

Keyhole welding, meaning that the laser beam forms a vapour cavity inside the steel, is one of the two types of laser welding processes and currently it is used in few industrial applications. Modern high power solid state lasers are becoming more used generally, but not all process fundamentals and phenomena of the process are well known and understanding of these helps to improve quality of final products. This study concentrates on the process fundamentals and the behaviour of the keyhole welding process by the means of real time high speed x-ray videography. One of the problem areas in laser welding has been mixing of the filler wire into the weld; the phenomena are explained and also one possible solution for this problem is presented in this study. The argument of this thesis is that the keyhole laser welding process has three keyhole modes that behave differently. These modes are trap, cylinder and kaleidoscope. Two of these have sub-modes, in which the keyhole behaves similarly but the molten pool changes behaviour and geometry of the resulting weld is different. X-ray videography was used to visualize the actual keyhole side view profile during the welding process. Several methods were applied to analyse and compile high speed x-ray video data to achieve a clearer image of the keyhole side view. Averaging was used to measure the keyhole side view outline, which was used to reconstruct a 3D-model of the actual keyhole. This 3D-model was taken as basis for calculation of the vapour volume inside of the keyhole for each laser parameter combination and joint geometry. Four different joint geometries were tested, partial penetration bead on plate and I-butt joint and full penetration bead on plate and I-butt joint. The comparison was performed with selected pairs and also compared all combinations together.

O avanço do processo de matematização na academia brasileira de economia desde a década de 1980

Looking at the economic discourse, we try to study in this article how has mathematization in economics advanced in Brazil in the last three decades. To see this, we have classified into several categories all articles published in three major economic journals of the country (Revista Brasileira de Economia, Estudos Econômicos and Revista de Economia Política) and the publications made in the meetings ANPEC from 1981 to 2010, according to the type of argument used. The total of articles analyzed adds up to 5.733. We try to see how the path of economic discourse, making it more mathematical, did develop. We found that there was an increased use of a formalized language from the mid-1990s onwards. Finally, to confirm our findings, we focus on the process of mathematization through the observation of quantitative variable: equations per article.

Le Petit journal. Supplément du dimanche

1904/12/04 (Numéro 733).

L'Écho de Paris

1886/03/15 (Numéro 733).

Mouvements d'assouplissements Miss Viola May : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 61105

Coislin 352

PHILARAS (Léonard). poésies grecques et latines

Informe Final: Evaluación de satisfacción del cliente interno en la CCB 2009, Vicepresidencia Eje Cívico Social (Gestión) Asocentro

Banco del conocimiento

Foundation

View of the construction of the foundations in November 1971.

Indenture between Joseph Jackson and William Woodruff (A)

Indenture of bargain and sale between Joseph Jackson of Buffalo, New York and William Woodruff of St. Davids regarding Lot no. 90 in St. Davids. This was registered in the County of Lincoln on April 30, 1842 in book B, folio 300. The instrument no. is 733. It was written 17 march 1842.

La sociología política

UANL