Report of the NBFGR-BOBLME Indian mackerel genetics harmonisation training workshop, Kochi, India, 20-27 August, 2013

This workshop was held at the National Bureau of Fish Genetic Resources and followed on from the Indian mackerel Working group meeting in Colombo (28-29 May, 2012). Activities included; DNA extraction; PCR (Polymerase Chain Reaction) for microsatellites; genotyping microsatellites; data analysis; emerging technologies; and an action plan

Report of the National Technical Workshop on the Conservation Strategy for the Myeik Archipelago, Dawei, Myanmar, 27 February, 2015

This was a follow up to the workshop held in October, 2014. This second workshop consolidated findings an and recommendations and highlighted the importance of cooperation between Department of Fisheries (DoF) and non-state actors.

Report of the Training Workshop on MPA Management Effectiveness Assessment Tool, Dhaka, Bangladesh, 27 and 29 April, 2015

Objectives of the workshop included; introduction to various Marine Protected Area (MPA) tools with a focus on Management Effectiveness Assessment Tool (MEAT); report on selected MPAs in Bangladesh; undertake initial assessments using MEAT; and develop workplans for other MPAs

两种鲿科鱼类在长江和珠江流域Cytb基因序列变异性分析

采用PCR技术获得了长江和珠江流域的两种科鱼类 1 1 37bpCytb基因的片段 ,进行了序列测定 ,并用Mega软件进行了序列分析。研究发现两种科鱼类的全序列从第十位碱基起有一个三联体密码子的缺失 ,推测为鲇形目鱼类Cytb序列所特有特征。黄颡鱼和大鳍种群间的序列差异分别为 0 .4 %和 1 .6 % ,介于一般淡水鱼类mtDNA种内序列的变异范围 ,而高于洄游鱼类序列变异。黄颡鱼属和属Cytb基因序列间的差异为 1 7.6 %— 1 8.0 % ,大大高于种间的序列差异率 ,也从分子水平上

Identification and characterization of a cathepsin L-like cysteine protease from Taenia solium metacestode

Taenia solium metacestode, a larval pork tapeworm, is a causative agent of neurocysticercosis, one of the most common parasitic diseases in the human central nervous system. In this study, we identified a cDNA encoding for a cathepsin L-like cysteine protease from the T solium metacestode (TsCL-1) and characterized the biochemical properties of the recombinant enzyme. The cloned cDNA of 1216 bp encoded 339 amino acids with an approximate molecular weight of 37.6 kDa which containing a typical signal peptide sequence (17 amino acids), a pro-domain (106 amino acids), and a mature domain (216 amino acids). Sequence alignments of TsCL-1 showed low sequence similarity of 27.3-44.6 to cathepsin L-like cysteine proteases from other helminth parasites, but the similarity was increased to 35.9-55.0 when compared to mature domains. The bacterially expressed recombinant protein (rTsCL-1) did not show enzyme activity; however, the rTsCL-1 expressed in Pichia pastoris showed typical biochemical characteristics of cysteine proteases. It degraded human immunoglobulin G (IgG) and bovine serum albumin (BSA), but not collagen. Western blot analysis of the rTsCL-1 showed antigenicity against the sera from patients with cysticercosis, sparganosis or fascioliasis, but weak or no antigenicity against the sera from patients with paragonimiasis or clonorchiasis. (c) 2006 Published by Elsevier B.V.

1.27μm吸收型部分增益耦合MQW-DFB激光器

采用LP-MOCVD和LPE相结合,成功地研制出了吸收型部分增益耦合MQW-DFB激光器。扫描显微镜照片显示了清晰的被掩埋的吸收型增益耦合光栅,表明光栅掩埋生长前升温过程磷烷的保护是成功的。宽接触(broad area)脉冲电流大范围单纵模工作,条型器件室温连续直流工作阈电流为22mA至35mA,单模成品率高,边模抑制比(SMSR)超过37dB,没有观察到饱和吸收现象。