Evidence for two protein coding transcripts at the Igf2as locus

The insulin-like growth factor 2 antisense (Igf2as) gene is part of the Ins-Igf2-H19 imprinted gene cluster. The function of the paternally expressed Igf2as is still elusive. In our previous work, we showed that Igf2as transcripts were located in the cytoplasm of C2C12 mouse myoblast cells, associated with polysomes and polyadenylated suggesting that Igf2as is protein coding. In the present work, the protein coding capacity of Igf2as was investigated. We demonstrate for the first time the existence of a polypeptide translated from an Igf2as construct. Furthermore, an RNA-Seq analysis was performed using RNA prepared from skeletal muscles of newborn wild-type and ∆ DMR1-U2 mice to further elucidate the function of Igf2as transcripts. We found no evidence for a regulatory role of Igf2as in the imprinted gene cluster. Interestingly, the RNA-Seq analysis indicated that Igf2as plays a role in the energy metabolism, the cell cycle, histone acetylation and muscle contraction pathways. Our Igf2as investigations further elucidated that there are two distinct Igf2as transcripts corresponding to two putative ORFs.

Quantitative assessment of sense and antisense transcripts from genes involved in antigenic variation (vsp genes) and encystation (cwp 1 gene) of Giardia lamblia clone GS/M-83-H7.

Antigenic variation of the intestinal protozoan parasite Giardia lamblia is caused by an exchange of the parasite's variant surface protein (VSP) coat. Many investigations on antigenic variation were performed with G. lamblia clone GS/M-83-H7 which produces surface antigen VSP H7. To generate novel information on giardial vsp gene transcription, vsp RNA levels were assessed by quantitative reverse transcription-(RT)-PCR in both axenic VSP H7-type trophozoites and subvariants obtained after negative selection of GS/M-83-H7 trophozoites by treatment with a cytotoxic, VSP H7-specific monoclonal antibody. Our investigation was not restricted to the assessment of the sense vsp transcript levels but also included an approach aimed at the detection of complementary antisense vsp transcripts within the two trophozoite populations. We found that sense vsp H7 RNA predominated in VSP H7-type trophozoites while sense RNA from only one (vsp IVg) of 8 subvariant vsp genes totally analysed predominated in subvariant-type trophozoites. Interestingly, the two trophozoite populations exhibited a similar relative distribution regarding the vsp H7 and vsp IVg antisense RNA molecules. An analogous sense versus antisense RNA pattern was also observed when the transcripts of gene cwp 1 (encoding cyst wall protein 1) were investigated. Here, both types of RNA molecules only appeared after cwp 1 had been induced through in vitro encystation of the parasite. These findings for the first time demonstrated that giardial antisense RNA production did not occur in a constitutive manner but was directly linked to complementary sense RNA production after activation of the respective gene systems.

Schools and religion : proceedings before the United States Commission on Civil Rights : executive summary and transcripts of proceedings held in Washington, DC, New York City, and Seattle, WA, Spring/Summer 1998.

"December 1999."

Rules of the United States Circuit Court of Appeals for the Fourth Circuit, 1930 : appendix, form of method of taking appeals, making up transcripts of records, docketing cases, printing records, etc.

Includes index.

Light and shadows on college athletes : college transcripts and labor market history /

"OR91-504"--P. [4] of cover.

Accessions of manuscripts, broadsides and British transcripts. July 1920/21-1925.

In continuation of lists published as appendices to annual reports of the librarian of Congress.

Minutes of the sessions of the Westminster assembly of divines while engaged in preparing their directory for church government, confession of faith, and catechisms (November 1644 to March 1649) From transcripts of the originals procured by a committee of the General assembly of the Church of Scotland;

"List of the members of the Westminster Assembly": p. [lxxxi]-lxxxvii.

Transcripts of hearings.

"HJR #68 and HJR #95."

The battle summer : being transcripts from personal observation in Paris, during the year 1848 /

Cover title: Reign of blouse.

The register book of christenings, weddings, and burials, within the parish of Leyland, in the county of Lancaster, 1653 to 1710. (With a few earlier "transcripts", 1622-1641.)

Contains the reports of the Record Society for the years 1888/9 and 1889/90.

Bishop's transcripts at Wells /

Vol. 7 included in v. 5 of: North Petherton registers.

Accessions of Manuscripts, Broadsides and British Transcripts

Mode of access: Internet.

Transcripts /

Thomas Guastello, Chairman.