Caracterização de superfície, adesiva e mecânica de uma zircônia parcialmente estabilizada por ítria em função da partícula ou do momento do jateamento

Pós-graduação em Reabilitação Oral - FOAR

Desempenho adesivo de sistemas de união à dentina aplicados in vivo e in vitro: efeito da pressão intrapulpar e profundidade dentinária

O objetivo do presente estudo foi investigar a influência da pressão intrapulpar e da profundidade dentinária sobre o desempenho adesivo de dois agentes de união à dentina, Single Bond (3M ESPE, St. Paul, MN, EUA) e Clearfil SE Bond (Kuraray, Tokyo, Japão), aplicados in vitro e in vivo. Quarenta e oito prémolares superiores hígidos foram selecionados e os pares pertencentes aos mesmos pacientes foram aleatoriamente distribuídos em 4 grupos experimentais de acordo com o sistema adesivo e a pressão intrapulpar, presente ou ausente. Dos dentes pertencentes ao mesmo par, um foi tratado in vivo e o outro in vitro. A ausência ou presença de pressão intra-pulpar foi determinada in vivo pelo uso de anestésicos locais com ou sem vasoconstritor, respectivamente. In vitro, os dentes foram mantidos sob pressão hidrostática de 15 cm de água por 24 horas. Cavidades de classe I foram preparadas e os sistemas adesivos aplicados de acordo com a recomendação dos fabricantes, seguidos da restauração incremental em resina composta. Para os dentes tratados in vitro, os mesmos procedimentos restauradores foram realizados após 6 meses de armazenagem em solução contendo timol 0,1%. Espécimes com área de secção transversal de 1 mm2 foram obtidos e submetidos ao ensaio mecânico de microtração. In vivo, ambos os sistemas adesivos apresentaram desempenho adesivo comparável, enquanto in vitro, o sistema Single Bond foi superior ao sistema Clearfil SE Bond. Esse último não foi influenciado por nenhuma das variáveis estabelecidas no estudo, ou seja, aplicação in vitro ou in vitro, presença de pressão intrapulpar e profundidade em dentina. O sistema Single Bond aplicado sob pressão intrapulpar positiva sofreu variação significante de resistência de união em função da profundidade da dentina, ou seja, em dentina profunda seu desempenho adesivo... (Resumo completo, clicar acesso eletrônico abaixo)

Proteins, Pathogens, and Failure at the Composite-Tooth Interface

In the United States, composites accounted for nearly 70% of the 173.2 million composite and amalgam restorations placed in 2006 (Kingman et al., 2012), and it is likely that the use of composite will continue to increase as dentists phase out dental amalgam. This trend is not, however, without consequences. The failure rate of composite restorations is double that of amalgam (Ferracane, 2013). Composite restorations accumulate more biofilm, experience more secondary decay, and require more frequent replacement. In vivo biodegradation of the adhesive bond at the composite-tooth interface is a major contributor to the cascade of events leading to restoration failure. Binding by proteins, particularly gp340, from the salivary pellicle leads to biofilm attachment, which accelerates degradation of the interfacial bond and demineralization of the tooth by recruiting the pioneer bacterium Streptococcus mutans to the surface. Bacterial production of lactic acid lowers the pH of the oral microenvironment, erodes hydroxyapatite in enamel and dentin, and promotes hydrolysis of the adhesive. Secreted esterases further hydrolyze the adhesive polymer, exposing the soft underlying collagenous dentinal matrix and allowing further infiltration by the pathogenic biofilm. Manifold approaches are being pursued to increase the longevity of composite dental restorations based on the major contributing factors responsible for degradation. The key material and biological components and the interactions involved in the destructive processes, including recent advances in understanding the structural and molecular basis of biofilm recruitment, are described in this review. Innovative strategies to mitigate these pathogenic effects and slow deterioration are discussed.

Influência da clorexidina na capacidade de umectabilidade da dentina hígida e afetada por cárie por um sistema adesivo

To evaluate the effect of chlorhexidine (CHX) on the wettability of sound and caries affected dentin by a simplified adhesive system. Material and Methods: Flat coronal dentin surfaces were produced on 60 sound molars, 30 of which were artificially decayed. The teeth were divided randomly into 3 groups (n = 10) with smear layer (SL), without SL impregnated with water and without SL impregnated with chlorhexidine. The SL removal was performed by phosphoric acid etching for 15 s. 20 uL of distilled water or 2% chlorhexidine digluconate were applied on the demineralized dentin for 60 s. Then, a drop of Single Bond 2 was deposited on each surface. Contact angles between dentin surface and adhesive was measured by means of a goniometer and data were submitted to ANOVA and Tukey tests (α = 0.05). Results: Higher contact angles were obtained on sound versus caries affected dentin (p <0.05), regardeless of the surface treatment. For both substrates, contact angles statistically higher were obtained for dentin covered with SL (P <0.05). The SL removal resulted in significant reduction of the angles (P <0.05) and no difference was found among angles produced on demineralized dentin impregnated with water or chlorhexidine (p> 0.05). Conclusion: Caries affected dentin wettability was higher than sound dentin and that characteristic was not influenced by chlorhexidine application.

Effect of surface treatments on the shear bond strength of luting cements to Y-TZP ceramic

Statement of problem Because zirconia is a glass-free material, alternative surface treatments such as airborne-particle abrasion or silica coating should be used for long-term bonding. However, these surface treatments in combination with different bonding agents and luting cements have not yet been studied. Purpose The purpose of the study was to evaluate the effect of surface treatments on the shear bond strength (SBS) of luting cements to Y-TZP ceramic. Material and methods Zirconia disks (N=240) were airborne-particle abraded with the following particles (n=48): 50 μm Al2O3; 120 μm Al2O3; 30 μm silica-coated Al2O3 (Rocatec Soft); 120 μm Al2O3+110 μm silica-coated Al2O3 (Rocatec Plus); and Rocatec Plus. After silanization of the zirconia surface, composite resin disks were bonded with (n=12) RelyX Luting 2; RelyX ARC; RelyX U100; and Panavia F. The bonded specimens were thermocycled (10 000 cycles) and tested for SBS. Failure mode was determined with a stereomicroscope (×20). The morphology and elemental composition of airborne-particle abraded surfaces were evaluated with scanning electron microscopy (×500) and energy-dispersive x-ray spectroscopy (×50). Results Surface treatments, cements, and their interaction were significant (P<.001). For RelyX ARC, Rocatec Soft and Rocatec Plus provided the highest SBS. In general, surface treatments did not influence the SBS of RelyX U100 and Panavia F. Regardless of the cement, no significant difference was found between 50 μm and 120 μm Al2O3 particles, between Rocatec Soft and Rocatec Plus, or between Rocatec Plus and 120 μm Al2O3 particles+Rocatec Plus. All groups showed adhesive failures. Different particle sizes provided differences in morphological patterns. The elemental composition comprised Al and Al/Si for alumina and silica-abraded zirconia. Conclusions Particle size did not influence the SBS of the groups abraded exclusively with alumina or silica-coated particles. RelyX ARC was more surface-treatment dependent than RelyX U100 or Panavia F.

The effect of CPP-ACP and Nd:YAG laser on the bond strength of softened dentin

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Adesivos multi-uso-avaliação da resistência adesiva a dentina frente a teste de cisalhamento, estereomicroscopia e microscopia eletrônica de varredura

Recent bonding systems have been advocated as multi-purpose bonding agents. The aim of this study was to determine if some of these bonding systems could be associated to composite resins from different manufacturers. This investigation was conducted to test lhe shear bond strength of three bonding systems: Scotchbond Multi-Purpose (3M Dental Products), Optibond Light Cure (Kerr) and Optibond Dual Cure (Kerr), when each of them was associated to lhe composite resins: Z1 00 (3M Dental Products), Prisma - APH (Dentsply) and Herculite XRV (Kerr). Seventy-two flat dentin bonding sites were prepared to 600 grit on human premolars mounted using acrilic resins. The teeth were assigned at random to 9 groups of 8 samples each. A split die with a 3mm diameter was placed over lhe surface of lhe dentin treated with one of lhe adhesive systems, and lhe selected composite resin was inserted and light cured. The split mold was removed and all samples were termocycled and stored in 37ºC water for 24 hours before testing. Shear bond strength was determined using an lnstron Universal testing machine. Some failures were examined under lhe S.E.M. Data was analysed by one-way analysis of variance, that demonstrated a significant difference (p<0,05) in the mean shear bond strength among Optibond Light Cure (15,446 MPa), Scotchbond Multi-Purpose (13,339 MPa) and Optibond Dual Cure (10,019 MPa). These values did not depend on the composite resin used. The association between bonding system/composite resin was statistycally significant (p<0,05) and the best results were obtained when the composite resins Z100 and Herculite were used with the adhesive system Optibond Light Cure, and when the composite resin APH was used with the adhesive system Scotchbond Multi-Purpose

Avaliação do efeito do plasma não térmico na resistência adesiva à dentina supericial e profunda

Pós-graduação em Odontologia Restauradora - ICT

Resistência de união de cimentos resinosos, convencional e autoadesivo, em substratos dentinários normal e hipermineralizado artificalmente

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of surface area and air-drying distance on shear bond strength of etch-and-rinse adhesive

We evaluated the effects of air-drying distance and bond surface area on the shear bond strength of a 2-step etch-and-rinse adhesive. A total of 120 bovine anterior teeth were equally divided into 6 main groups based on bonding surface area. The main groups were divided into sub-groups (n = 5) according to air-drying distance. The shear strength was determined using a universal testing machine at a crosshead speed of 0.5 mm/min. The averaged results were subjected to two-way ANOVA and Tukey's test (alpha = 0.05). Two-way ANOVA testing identified no significant cross-product interactions (p > 0.05), but the main factors of area (p < 0.0001) and air-drying distance (p < 0.00001) significantly affected the mean bond strength. Shorter air-drying distances improved bond strength, and increased surface area decreased the bond strength.

Influence of Ozone Gas and Ozonated Water Application to Dentin and Bonded Interfaces on Resin-Dentin Bond Strength

Purpose: To assess the influence of ozone gas and ozonated water application to prepared cavity and bonded interfaces on the resin/dentin bond strength of two-step etch-and-rinse adhesive systems (Adper Single Bond 2 [SB2] and XP-Bond [XP]). Materials and Methods: Sixty extracted human third molars were sectioned perpendicularly to their long axes to expose flat occlusal dentin surfaces. In experiment 1, dentin was treated with ozone before the bonding procedure, while in experiment 2, ozone was applied to resin/dentin bonded interfaces. In experiment 1, dentin surfaces were treated either with ozone gas (2100 ppm), ozonated water (3.5 ppm), or distilled water for 120 s, and then bonded with SB2 or XP according to manufacturers' instructions. Hybrid composite buildups were incrementally constructed and the teeth were sectioned into resin-dentin sticks (0.8 mm(2)). In experiment 2, dentin surfaces were first bonded with SB2 or XP, composite buildups were constructed, and bonded sticks obtained. The sticks were treated with ozone as previously described. Bonded sticks were tested under tensile stress at 1 mm/min. Silver nitrate impregnation along the resin/dentin interfaces was also evaluated under SEM. Results: Two-way ANOVA (adhesive and ozone treatment) detected no significant effect for the cross-product interaction and the main factors in the two experiments (p > 0.05), which was confirmed by the photomicrographs. Conclusion: Ozone gas and ozonated water used before the bonding procedure or on resin/dentin bonded interfaces have no deleterious effects on the bond strengths and interfaces.

Shear bond strength of resin cements to human dentin

OBJECTIVES: The objectives of this in vitro study were (1) to assess the bond strength of the universal cement RelyX Unicem to dentin and to compare it with three conventional resin cements, (2) to test the influence of aging on their bonding capacity and (3) to test the influence of the operator on bonding quality by performing the same test in two different centers. METHODS: 160 third molars, divided into 80 for tests at the University of Zurich (Z) and 80 for tests at the University of Berne (B), were assigned to 2 x 8 subgroups of 10 teeth each. The specimens were prepared with the corresponding bonding agents and acrylic rods were luted either with RelyX Unicem (U), RelyX ARC (A), Multilink (M) or Panavia 21 (P). All specimens were stored in water for 24h (W) and half of the specimens were subjected to 1500 cycles of thermocycling (5 degrees C and 55 degrees C) (T). Bond strength was measured by means of a shear test. RESULTS: After water storage RelyX Unicem exhibited lowest bond strength (UWZ: 9.2+/-1.6 MPa, UWB: 9.9+/-1.2 MPa, AWZ: 15.3+/-6.0 MPa, AWB: 12.2+/-4.3 MPa, MWZ: 15.6+/-3.3 MPa, MWB: 12.4 MPa+/-2.4, PWZ: 13.4+/-2.9 MPa, PWB: 14.9+/-2.6 MPa). Thermocycling affected the bonding performance of all four cements. However, bond strength of RelyX Unicem was least influenced by thermocycling (UTZ: 9.4+/-2.9 MPa, UTB: 8.6+/-1.3 MPa, ATZ: 11.4+/-6.3 MPa, ATB: 13.3+/-3.7 MPa, MTZ: 15.4+/-3.1 MPa, MTB: 10.3+/-2.4 MPa, PTZ: 11.1+/-2.8 MPa, PTB: 11.3+/-2.8 MPa). SIGNIFICANCE: Although the bond strength of RelyX Unicem to dentin was lower in comparison to RelyX ARC, Multilink and Panavia 21, its bond strength was less sensitive to variations in handling and aging.

Protocolos para a colocação e remoção de espigões

A utilização de espigões em dentes tratados endodonticamente é um dos temas mais estudados em Medicina Dentária. As opiniões são divergentes em relação aos procedimentos clínicos e materiais a serem utilizados para a colocação e remoção de espigões. O objetivo deste trabalho foi realizar uma revisão bibliográfica de forma a organizar conceitos e princípios clínicos para melhor esclarecer os fatores que determinam a necessidade de colocação, utilização e escolha do tipo de espigão, sua cimentação e técnicas para a remoção. Foram analisadas as características e propriedades dos cimentos de fosfato de zinco, ionômero de vidro, cimentos resinosos de polimerização química, polimerização dupla, foto-polimerizável e os sistemas adesivos etch and rinse, self etch e autoadesivos, bem como as técnicas para a remoção de espigões cimentados com diferentes cimentos e sistemas adesivos para depois acessar o remanescente de guta percha para o retratamento endodôntico. Foi feita uma pesquisa bibliográfica na base de dados electrónica PubMed, Google Scholar e RCAAP com as seguintes palavras chave: “Espigões”; “Retratamento Endodôntico”; “Ionómero de Vidro”; “Fosfato de Zinco”; “Cimentos Resinosos”; “Posts”; “Endodontic Retreatment”; “Glass Ionomer”; Zinc-phosphate”; “Resin Cements"; “Push Out Test”; “Posts AND Removal”. Concluiu-se que a cimentação de espigões pré-fabricados de fibra de vidro com cimentos resinosos de dupla polimerização associados aos sistemas adesivos self etch estão gradualmente substituindo os outros tipos de espigões e demais cimentos e possibilitam restaurar o dente de forma adequada e duradoura. E o uso de ultrassons apresenta maior eficácia e segurança na remoção dos espigões.

Sodium hypochlorite effects on dentin bond strength and acid-base resistant zone formation by adhesive systems

Aim: To evaluate the effects of 10% NaOCl gel application on the dentin bond strengths and morphology of resin-dentin interfaces formed by three adhesives. Methods: Two etch-and-rinse adhesives (One-Step Plus, Bisco Inc. and Clearfil Photo Bond, Kuraray Noritake Dental) and one self-etch adhesive (Clearfil SE Bond, Kuraray Noritake Dental) were applied on dentin according to the manufacturers’ instructions or after the treatment with 10% NaOCl (ED-Gel, Kuraray Noritake Dental) for 60 s. For interfacial analysis, specimens were subjected to acid-base challenge and observed by SEM to identify the formation of the acid-base resistant zone (ABRZ). For microtensile bond strength, the same groups were investigated and the restored teeth were thermocycled (5,000 cycles) or not before testing. Bond strength data were subjected to two-way ANOVA and Tukey’s test (p<0.05). Results: NaOCl application affected the bond strengths for One-Step Plus and Clearfil Photo Bond. Thermocycling reduced the bond strengths for Clearfil Photo Bond and Clearfil SE Bond when used after NaOCl application and One-Step Plus when used as recommended by manufacturer. ABRZ was observed adjacent to the hybrid layer for self-etch primer. The etch-and-rinse systems showed external lesions after acid-base challenge and no ABRZ formation when applied according to manufacturer’s instructions. Conclusions: 10% NaOCl changed the morphology of the bonding interfaces and its use with etch-&-rinse adhesives reduced the dentin bond strength. Formation of ABRZ was material-dependent and the interface morphologies were different among the tested materials.

Influence of adhesive system on quartz fiber post dislocation resistance in endodontically treated teeth

Aim: To evaluate the dislocation resistance of the quartz fiber post/cement/dentin interface after different adhesion strategies. Methods: Forty bovine lower central incisors were selected and prepared with K-files using the step-back technique, and irrigated with 3 mL of distilled water preceding the use of each instrument. Prepared teeth were stored at 37ºC and 100% humidity for 7 days. The roots were prepared and randomized into 4 groups. The quartz fiber post was cemented with an adhesion strategy according to the following groups: GBisCem- BISCEM; GOneStep±C&B- One Step ± C&B; GAllBond±C&B- AllBond3 ± C&B; GAllBondSE±C&B- AllBondSE ±C&B with a quartz fiber post. Cross-sectional root slices of 0.7 mm were produced and stored for 24 h at 37° C before being submitted to push-out bond strength. Results: The mean and standard deviation values of dislocation resistance were GBisCem: 1.12 (± 0.23) MPa, GOneStep±C&B: 0.81 (± 0.31) MPa, GAllBond±C&B: 0.98 (± 0.14) MPa, and GAllBondSE±C&B: 1.57 (± 0.04) MPa. GAllBondSE±C&B showed significantly higher values of dislocation resistance than the other groups. Conclusions: Based on this study design, it may be concluded that adhesion strategies showed different results of quartz post dislocation resistance. Simplified adhesive system with sodium benzene sulphinate incorporation provided superior dislocation resistance.