High-affinity RNA-binding domains of alfalfa mosaic virus coat protein are not required for coat protein-mediated resistance.

A virus-based vector was used for the transient expression of the alfalfa mosaic virus coat protein (CP) gene in protoplasts and plants. The accumulation of wild-type CP conferred strong protection against subsequent alfalfa mosaic virus infection, enabling the efficacy of CP mutants to be determined without developing transgenic plants. Expression of the CP mRNA alone without CP accumulation conferred weaker protection against infection. The activity of the N-terminal mutant CPs in protection did not correlate with their activities in genome activation. The activity of a C-terminal mutant suggested that encapsidation did not have a role in protection. Our results indicate that interaction of the CP with alfalfa mosaic virus RNA is not important in protection, thereby leaving open the possibility that interactions with host factors lead to protection.

Non-clathrin-coat protein alpha is a conserved subunit of coatomer and in Saccharomyces cerevisiae is essential for growth.

To complete the molecular characterization of coatomer, the preformed cytosolic complex that is involved in the formation of biosynthetic transport vesicles, we have cloned and characterized the gene for non-clathrin-coat protein alpha (alpha-COP) from Saccharomyces cerevisiae. The derived protein, molecular weight of 135,500, contains four WD-40 repeated motifs (Trp/Asp-containing motifs of approximately 40 amino acids). Disruption of the yeast alpha-COP gene is lethal. Comparison of the DNA-derived primary structure with peptides from bovine alpha-COP shows a striking homology. alpha-COP is localized to coated transport vesicles and coated buds of Golgi membranes derived from CHO cells.

Francesco d'Este (born about 1430, died after 1475), reverse, Este family coat of arms and crest

Rogier van der Weyden; 11 47/64 in.x 7 63/64 in. (painted surface); oil on wood

How to make a coat /

Mode of access: Internet.

A loyal little red-coat : a story of child-life in New York a hundred years ago /

Mode of access: Internet.

The book containing the treatises of hawking, hunting, coat-armour, fishing, and blasing of arms; as printed at Westminster by Wynkyn de Worde ... 1496.

Introd. and commentary by Joseph Haslewood.

Hawaii's great seal and coat of arms /

Mode of access: Internet.

A blue-coat boy's recollections of Hertford school.

Mode of access: Internet.

Recollections of a blue-coat boy, or, A view of Christ's Hospital.

Chau & Chau. Blunden

The book containing the treatises of hawking, hunting, coat-armour, fishing, and blasing of arms : as printed at Westminster, by Wynkyn de Worde, the year of the Incarnation of our Lord MCCCClxxxxvi.

Includes indexes.

Virginia heraldica, being a registry of Virginia gentry entitled to coat armor, with genealogical notes of the families;

Mode of access: Internet.

Tailgating, woman in striped coat

[wire service photo]