Evaluation of memory B cells among perinatally HIV infected children from Houston Texas

Background: HIV associated B cell exhaustion is a notable characteristic of HIV viremic adults. However, it is not known if such alterations are present in perinatal HIV infected children, whose viral dynamics differs from those seen in adults. In the present study we perform an analysis of B cells subsets and measure antigen-specific memory B cells (MBC) in a pediatric HIV infected cohort. ^ Methods: Peripheral mononuclear cells (PBMC) of perinatal HIV infected individuals are characterized into naïve (CD21hi/CD27−), classic (CD27+), tissue like (CD21lo/CD27 −) and activated MBC (CD27+CD21− ) by FACS. A memory ELISPOT assay is used to detect antibody secreting cells. We measure total IgG and antibodies specific for influenza, HBV, mumps, measles, rubella and VZV. Memory was expressed as spot forming cells (SPC) /million of PBMC. Wilcoxon rank-sum was used to compare unpaired groups and linear regression analysis was used to determine predictors of B cell dysfunction ^ Results: 41 HIV perinatal infected children are included (51.2% females and 65.9% Black). Age at study is median (range) 8.78 years (4.39-11.57). At the time of testing they have a CD4% of 30.9 (23.2-39.4), a viral load (VL) of 1.95 log10 copies/ml (1.68-3.29) and a cumulative VL of 3.4 log10 copy × days (2.7-4.0). Ninety two percent of the children are on cARV for > 6 months. Overall, HIV+ children compared with controls have a significant lower number of IgG and antigen specific SFC. In addition, they have a lower proportion of classical MBC 12.9 (8.09-19.85) vs 29.4 (18.7-39.05); 0.01, but a significant higher proportion of tissue like memory MBC 6.01 (2.79-12.7) vs 0.99 (0.87-1.38); 0.003, compared with controls. Patients are parsed on VL (<400 and ≥ 400 copies/ml) with the objective to evaluate the effect of VL on B cell status. Patients with a VL ≥ 400 copies/ml have a significantly lower IgG, HBV, measles, rubella and VZV SPC compared with those with a VL < 400 copies/ml. There are no significant differences in B cell subpopulations between the groups. A moderate negative correlation was observed between the time of cARV initiation and the frequency of IgG memory B cells, suggesting that early initiation of cARV appears to lead to a better functionality of the IgG memory B cells (P=0.05). A statistically significant positive correlation was observed between the total number of IgG memory cells and the number of antigen-specific memory B cells/SPCs. Suggesting that the progressive recovery of the IgG memory B cell pull goes along with a progressive increase in the number of antigen-specific SPCs. ^ Conclusion: A pediatric cohort in overall good status with respect to HIV infection and on ART has defects in B cell function and numbers (reduced total and antigen specific MBC and increased tissue like and reduced classical MBC).^

The Museum/School Partnership: A New Role for Art Museums in K-5 Art Education

For nearly thirty years, the arts have been poorly represented in public school classrooms due to tight budgets, state mandates, and a belief that the arts are not essential to education. In this paper, I will investigate the absence of focused art education curriculum in K-5 classrooms across the United States’ public school system, explain the advantages of reinstating art as a basic subject in the classroom curriculum, and advocate for a more active art museum role in public school elementary art education. The art museum may be in the ideal position to help develop and facilitate programming in K-5 classrooms. By placing teams of art museum professionals in public school classrooms, art museums can establish a prominent role in the museum/school relationship and can help ensure that children have adequate access to art education. The outcome would be children who have greater academic and personal successes throughout their lives.

Olhares street art - investigação ação : de que forma abordagens de arte contemporânea nas escolas promovem a criatividade

In this study we intend to address a learning experience that was inspired by the intersection between public art and art education. We intend to make a reflection and analysis of pedagogical actions undertaken during this experience related to a theoretical framework to help us evaluate and improve the pedagogical and methodological strategies. The experience began with a visit to the exhibition "Street Art" at the AXA building in Porto. This visit was made with a group of children of different ages, accompanied by their parents, in May 2014. This exhibition brought new gazes for urban art. By recognizing obvious benefits such as proximity and accessibility, urban art, so rarely addressed in the classroom, may be an educational resource. Our intention in this study asks whether the contact with urban works of art can be motivating for students and facilitators of learning. We used a methodology of action research to approach urban art in visual art education lessons, exploring various art objects of the exhibition "Street Art" and the surrounding city with students. The study findings pointed out a positive response to initial questions

Madame Georges Charpentier (Marguérite-Louise Lemonnier, 1848–1904) and Her Children, Georgette-Berthe (1872–1945) and Paul-Émile-Charles (1875–1895)

Pierre-Auguste Renoir; 5 ft. 33/64 in. x 6 ft. 2 7/8 in.; oil on canvas

The Calmady Children (Emily, 1818–?1906, and Laura Anne, 1820–1894)

Sir Lawrence Thomas; 2 ft. 6 55/64 in.x 2 ft. 6 1/8 in.; oil on canvas

Mrs. Noah Smith and Her Children

Ralph Earl; 5 ft. 4 1/64 in.x 7 ft. 1 3/4 in.; oil on canvas

The library of home economics : a complete home-study coursr on the new profession of home-making and art of right living : the practical application of athe most recent advances in the arts and sciences to home and health /

v.1. The house, its plan, decoration and care / by Isabel Bevier. -- v.2. Household bacteriology / by S. Maria Elliott. -- v.3. Household hygiene / by S. Maria Elliott. -- v.4. Chemistry of the household / by Margaret E. Dodd. -- v.5. Principles of cookery / by Anna Barrows. -- v.6. Food and dietetics / by Alice P. Norton. -- v.7. Household management by Bertha M. Terrill. -- v.8. Personal hygiene / ed. by M. Le Bosquet. -- v.9. Home care of the sick / by Amy E. Pope. -- v.10. Textiles and clothing / by Kate H. Watson. -- v.11. Study of child life / by Marion F. Washburne. -- v.12. Care of children / by A.C. Cotton.

Creative crafts with elementary children.

Mode of access: Internet.

Art + Bioéthique : quand la recherche en bioéthique quitte les murs de l’université

Éditoral / Editorial

Art + Bioéthique : quand la recherche en bioéthique quitte les murs de l’université

Éditoral / Editorial

Parental reports of the oral health-related quality of life of children with cerebral palsy

BACKGROUND The severity of physical and mental impairments and oral problems, as well as socioeconomic factors, may have an impact on quality of life of children with cerebral palsy (CP). The aim of this research was to assess the impact of impairments and oral health conditions, adjusted by socioeconomic factors, on the Oral Health-Related Quality of Life (OHRQoL) of children with CP using their parents as proxies. METHODS Sixty children, between 6-14 years of age were selected. Their parents answered a children's OHRQoL instrument (5 domains) which combines the Parental-Caregivers Perception Questionnaire (P-CPQ) and Family Impact Scale (FIS). The severity of dental caries, type of CP, communication ability, gross motor function, seizures and socioeconomic conditions were assessed. RESULTS Considering the total score of the OHRQoL instrument, only the reduction of communication ability and dental caries severity had a negative impact on the OHRQoL (p < 0.05). Considering each domain of the instrument, the severity of the type of CP and its reduction of communication ability showed a negative impact on oral symptoms and functional limitations domains (p < 0.05). Seizures have a negative impact on oral symptoms domain (p = 0.006). The multivariate fitted model showed that the severity of dental caries, communication ability and low family income were negatively associated with the impact on OHRQoL (p = 0.001). CONCLUSIONS The severity of dental caries, communication ability, and family income are conditions strongly associated with a negative impact on OHRQoL of children with CP.