NMR spectrum of lindgomycin from a Marine Fungus of the Lindgomycetaceae

An unusual polyketide with a new carbon skeleton, lindgomycin (1), and the recently described ascosetin (2) were extracted from mycelia and culture broth of different Lindgomycetaceae strains, which were isolated from a sponge of the Kiel Fjord in the Baltic Sea (Germany) and from the Antarctic. Their structures were established by spectroscopic means. In the new polyketide, two distinct domains, a bicyclic hydrocarbon and a tetramic acid, are connected by a bridging carbonyl. The tetramic acid substructure of compound 1 was proved to possess a unique 5-benzylpyrrolidine-2,4-dione unit. The combination of 5-benzylpyrrolidine-2,4-dione of compound 1 in its tetramic acid half and 3-methylbut-3-enoic acid pendant in its decalin half allow the assignment of a new carbon skeleton. The new compound 1 and ascosetin showed antibiotic activities with IC50 value of 5.1 (±0.2) µM and 3.2 (±0.4) µM, respectively, against methicillin-resistant Staphylococcus aureus.

An Arginine Deprivation Response Pathway Is Induced in Leishmania during Macrophage Invasion

Amino acid sensing is an intracellular function that supports nutrient homeostasis, largely through controlled release of amino acids from lysosomal pools. The intracellular pathogen Leishmania resides and proliferates within human macrophage phagolysosomes. Here we describe a new pathway in Leishmania that specifically senses the extracellular levels of arginine, an amino acid that is essential for the parasite. During infection, the macrophage arginine pool is depleted due to its use to produce metabolites (NO and polyamines) that constitute part of the host defense response and its suppression, respectively. We found that parasites respond to this shortage of arginine by up-regulating expression and activity of the Leishmania arginine transporter (LdAAP3), as well as several other transporters. Our analysis indicates the parasite monitors arginine levels in the environment rather than the intracellular pools. Phosphoproteomics and genetic analysis indicates that the arginine-deprivation response is mediated through a mitogen-activated protein kinase-2-dependent signaling cascade.

Cheerleaders, UM Football 1955 vs Indiana

L-R: Jack Campbell, Nico Wiese, ?, Dick Kimball, Tom Taylor, Ed Gagnier,? (IDs tentative based on 1956 photo)

Cheerleaders, UM Football 1956

Standing, L-R: Tom Taylor, Jim Hayslett, Dana Larson, co-captain Ralph Watts jr. Dick Kimball, Jack Campbell, Ed Gagnier; Seated: co-captain Nico Wiese

The Yangtze: China's river highway.

Describes life along the river whose basin supports one-tenth of the human race.

Lucky days for Johnny /

Johnny and his family invite his teacher, Miss Day, to their home for dinner on Friday evening. Johnny has a wonderful week-end because of the dinner.

Urkundenbuch der Stadt Wetzlar.

1.Bd. 1141-1350, bearb. E. Wiese.--2.Bd. 1214-1350, bearb. M. Sponheimer.

De M. Val. Messallae Corvini vita et studiis doctrinae ...

Vita.

Friedrich Wilhelm, Graf v. Goetzen : Schlesiens Held in der Franzosenzeit, 1806 bis 1807.

Mode of access: Internet.

Atomic transition probabilities; a critical data compilation

Mode of access: Internet.

Die Desinfektionskraft des Magensaftes gegenüber pathogenen Mikroorganismen.

Inaug.-diss.--Hannover, 1912.

Commentatio de vitarum scriptoribus Romanis,

On reel 272 beginning frame no. 160.

Moses : Drama.

Mode of access: Internet.

1942 University of Michigan Football Team

1943 University of Michigan Football Team