Seleção e divergência genética de pessegueiros e nectarineiras e susceptibilidade a danos causados pelo frio na Estación Experimental de Aula Dei (CSIC)

The peaches and nectarines are highly appreciated by consumer, but it is climacteric fruits, with availability in the market in small time. It is necessary to invest to obtain genotypes with fruit quality and small perishability or that it presente less physiological disorders after storage. The aims of this work were i) to evaluate the genetic divergence among 40 peach and nectarine trees genotypes based on postharvest quality and select posible parents; ii) to evaluate the susceptibility to chilling injury in peaches and nectarines after cold storage; iii) to evaluate divergence of peaches and nectarines on the basis in the susceptibility for chiling injury and select superior genotypes; iv) evaluate the correlations between quality and susceptibility to chilling injury of peaches and nectarines v) select parents with the combination of lower susceptibility to chilling injury and higher quality fruit. The study was carried out in EEAD-CSIC, Zaragoza - Spain, during the production cycle 2013/2014. A total of 40 peaches and nectarines genotypes from germplasm collection were evaluated. The quality characteristics as flesh firmness, total soluble solids, titratable acidity, pH, rippining index and flesh color parameters were evaluated. The fruits were submitted to cold storage at 0 °C and 5 °C, with 95% average relative humidity. The evaluations were after 14 and 28 days, it being observed the presence of symptoms, such as wooliness through mealiness, flesh grainy, leatheriness and flesh color changes, through browning, bleeding and off flavor. As a selection parameter was adopted 20% of genotypes that had a higher frequency of superiority for quality characteristics, susceptibility to chilling injury and the combining of both. For quality characteristic presented greater divergence the ‘Queen Giant’, ‘Sudanel Blanco’ and ‘Borracho de Jarque’. Based on the quality the eight genotypes were selected, ‘Andross’, ‘San Jaime’, ‘San Lorenzo’, ‘Borracho de Jarque’, ‘Sudanell 1’, ‘Carson’, ‘Baby Gold 6’ and ‘Stanford’. All genotypes studied exhibited susceptibility to one or more symptoms caused by cold storage during 28 days, independent of temperature. For 14 days, the ‘Baby Gold 6’, ‘Flavortop’ and ‘Queen Giant’ genotypes did not show any physiological disorder caused by cold. In general, the temperature of 0 °C favored fruit postharvest conservation, it have a lower incidence and severity of symptoms caused by cold storage. The storage for 14 days contributed for the lower incidence of damage in the genotypes fruits studied. For 14 days, with both temperatures, it was observed divergence for ‘Queen Giant’, ‘Sudanell Blanco’, ‘Baby Gold 6’ ‘GF3’, ‘Baby Gold 8’, ‘Campiel’ and ‘Campiel Rojo’ genotypes. For 28 days, in the 5 °C condition, ‘Queen Giant’, ‘Big Top’, ‘Flavortop’ and ‘Redhaven’ genotypes were divergents. Based on susceptibility to chilling injury at 0 °C, the eight genotypes were selected, it being these, ‘Queen Giant’, ‘Keimoes’, ‘Flavortop’, ‘Big Top’, 'Redhaven', 'Sudanell 3', 'Bonet I' and ‘Carson’. The quality parameters as rippining index, soluble solids, firmness and titratable acidity presented correlation among them. These, also it had correlation with woolines and bowning, what it indicate that fruits with more ripening can have this symptoms more easily. The browning, mealiness, flesh grainy and off flavor variables were correlationed with the time period and temperartures, what it confirm that these symptoms are the main disorders caused by cold storage. The quality characteristics together susceptibility to chilling injury allowed selected ‘Baby Gold 6’, ‘Sarell’, ‘Keimoes’, ‘GF3’ ‘San Jaime’, ‘Big Top’, ‘Sudanell 1’, ‘Carson’, ‘Baby Gold 8’, and ‘San Lorenzo’ genotypes.

Avaliação inorgânica de material particulado atmosférico inalável na região norte do Paraná

This study aimed to determine the concentration and inorganic chemical composition of samples from airborne particulate matter inhaled in fine and coarse fractions. Aerosol samples were collected in 2013 and 2014, from sites located in the cities of Londrina and Maringa, in the state of Paraná, Brazil. The samples were collected daily (24h) in two campaigns: winter and summer. For the collection, was used a dichotomous sampler with quartz fiber filter with 47 mm in size and 2 µm porosity, 97% efficiency, retaining particles of up to 0.3 µm. Quantification of the airborne particulate matter mass was performed by gravimetry method. The results from Londrina to PM2.5 and MP2,5-10 represent, respectively, 29.2% and 70.8% of airborne particulate matter in the winter campaign (2013), 30.9% (PM2.5) and 69.1 % (MP2,5-10) in the summer campaign (2013), and 35.9% (PM2.5) and 64.1% (MP2,5-10) in the winter 2014 campaign. In the city of Maringa, the results presented the percentage of 42.0% (PM2.5) and 58.0% (MP2,5-10) for the winter season (2014), and 28.8% (PM2.5) and 71.2 % (MP2,5-10) for the summer season (2014). The PM2.5/PM10 ratio was on average 0.3, demonstrating that both cities are developing urban areas. Analysis of the major soluble inorganic species in water (NO3-, SO42- and Cl-) associated with MP2,5-10 were quantified by ion chromatography at the LACA Laboratory in the State University of Londrina, with the largest contribution found in all campaigns was to NO3-. The NO3-/SO42- ratios above 1.0 indicate the local traffic contribution. The analysis of metals associated with PM2.5 was carried out by mass spectrometry with inductively coupled plasma (ICP-MS) in the Federal University of Santa Catarina. The Zn, Pb, Cu and Mn concentrations found in all campaigns indicate the contribution of mobile sources to PM2.5. The concentration of BCe in PM2.5 was determined by reflectance, with higher BCe concentrations being found in winter campaigns. In general, Londrina presented the highest concentrations from the species analyzed when compared to Maringá. In addition, the analysis of the air mass trajectories indicated the transportation of pollutants coming mainly from fires in the southeastern region of the country.

Estudo de caso: minimização e reúso de água em shopping center da região sul do Brasil

The main issues related to water conservation in urban centers are the increase in water supply cost, demand growth, pollution and differences in the distribution of water resources. Water conservation, the controlled and efficient use of water, includes both measures as reasonable means of water reuse. Thus, conservation practices are an effective way to meet demand and supply water to new activities and users without jeopardizing the supplying water bodies and preserving the natural environment. This study aims to examine the water management of a shopping mall and the use of rainwater harvesting combined with greywater reuse. For buildings in general, water loss is common due to leaks in the hydraulic and restroom equipment. These losses, which are caused by a high volume of water used and wasted in the system, are often the result of design errors, incorrect maintenance procedures and users' bad habits In southern Brazil, where there is rainfall almost all year long, water shortages occasionally occur, particularly in some winter mouths. One difficulty that appears on rainwater studies is the proper determination of rainwater volume that can be used to address water supply systems. In this work, the simulation method was used to determine this volume. Thus, simulations with the following variables: rainfall, catchment area and water consumption were performed. For mall's hydraulic systems, segmented alternatives are adopted. That is, focusing on the use of rainwater or greywater reuse. Other alternatives of effluent reuse have been slightly discussed due to sanitary issues, those are effluents from toilets and kitchen sinks. The adoption of greywater may be feasible if there is a significant flow of greywater to comply water demand for toilet flushing. The inspections made in this study found that the quantity of sinks was insufficient to supply an adequate amount of water to toilets and urinals. The greywater reuse system was found to be infeasible in terms of demand and supply of water. Conversely, the rainwater harvesting system was entirely feasible and easily supplied water to all restrooms and contributed to the cooling of the air conditioning system with a short payback period. One of the challenges of this work was the need to compare the actual water consumption with a water consumption parameter used in buildings. Thus, a method that addresses the generation of specific consumption indexes for specific activity (like a mall) was used. The water consumption indices showed that this mall has a satisfactory water management program.

Fauna edáfica sob modelos em estágio inicial de restauração de floresta subtropical

The goal of this study was to compare areas under different forest restoration technologies in relation to abundance, richness, diversity and composition of the present fauna in the litter and soil. The treatments evaluated were: natural regeneration (RN); high diversity tree plantations (L) and nucleation (N). An area of secondary forest was included in the study as a reference of soil conditions. The experimental design was in randomized block with four replications. Samples were collected for extraction of mesofauna (October/2012; July and October/2013) and macrofauna (June and October/2013) in six points of each plot, totaling 24 samples per treatment. For collecting soil macrofauna was used TSBF method. The mesofauna was collected with a metal cylinder and extracted by Berlese-Tüllgren funnel. Litter and soil were collected separately at each point and the fauna was identified level of class/taxonomic order. The springtails were classified using morphotypes. In total, considering the mesofauna, macrofauna and three times collected were accounted 28618 organisms. In relation soil mesofauna, the evaluated technologies did not differ, after three years of restoration, in relation to total abundance of organisms and community composition. The Shannon diversity index (H), in soil mesofauna, followed a human impact gradient. This index was higher in natural regeneration, which not was undergone technical interventions and showed higher moisture in the soil. The tree planting technology, under the control of volunteer plants in total area, showed lower H index. In the case of litter mesofauna, the technologies did not differ in relation the mean richness, total abundance of organisms and community composition. Considering edaphic macrofauna, technologies did not differ in relation to the abundance and richness, and in the evaluation of June/2013, RN showed higher H index and differed in relation to the community composition of other technologies. In October/2013 evaluation, the differences between the technologies in relation to H index were narrower and these did not differ in terms of composition of soil macrofauna community. In litter macrofauna, in June/2013, the RN presented greater richness and H index when compared to other technologies and in evaluation October/2013 technologies did not differ in relation to community composition, richness and mean abundance of organisms. In the case of springtails, technologies after three years in the restoration process, did not differ in relation to the abundance, richness and composition of Collembola community for different morphotypes. The secondary forest, in relation to forest restoration technologies, presented greater abundance of saprophages, predators and greater diversity of morphotypes of springtails. From these results it, we recommended to natural regeneration by to have the lowest cost of deployment, followed by nucleation and online planting. The animals should be monitored over time, in the restoration technologies, as well as the physical and chemical characteristics of the soil, in order to understand the possible changes in the composition and diversity of organisms.

Padrões de organização das assembleias de aves florestais em uma paisagem fragmentada de Floresta Atlântica

Brazil is home to one of the richest avifaunas the world, which is subject to high levels of environmental degradation, in particular forest fragmentation. The Atlantic Forest biome depicts this history of devastation and today remains as small isolated fragments on highly degraded landscapes. This project aimed to evaluate the effects of forest fragmentation in an area with Atlantic Forest remnants in northern Paraná (Brazil) on the distribution and the organization of assemblage of forest birds and tested the hypothesis that the structure of the assembly in the fragments is different than expected by chance. We did four qualitative samplings of birds in three sets of forest fragments in the landscape, each with three fragments: large, medium and small. The method applied in the sampling was point counts along transects, traveled randomly for four hours in each fragment. Samples were taken in two periods: from September to November / 2013, and between March and May / 2014. The structure of the meeting was assessed by rates of co-occurring species (Checkerboard and CScore) and α diversity patterns (wealth) and β (turnover of species), while the landscape structure was analyzed from the parameters: area, distance between fragments, fractal dimension, edge density, fragment shape index and nuclear area index. The null hypothesis of no structure in the assembly of birds in the landscape was tested with null models from the co-occurrence indexes. The effects of landscape structure on the assembly of the structure were analyzed by the Mantel test and principal component analysis (PCA). The assembly of the structure in the landscape showed a pattern of spatiotemporal organization significantly different from that expected by chance, revealing a structure most influenced by segregation of the species. The fragments showed significant differences in richness, unlike sets of fragments, indicating relative homogeneity in the landscape structure. The differences between the size and the distance between the fragments significantly influenced the patterns of organization of the meeting of forest birds in the landscape and patterns of α and β diversity, indicating that the higher the fragment and smaller distances between them, more the standard of species cooccurrence is different than expected by chance. Thus, the fragmented landscape of remnants of the northern Paraná Atlantic Forest still has availability of environmental resources and physical characteristics that allow a persistent organizational structure of the assembly of forest birds in space over time.

Estrutura e dinâmica de populações de Hydrochoerus hydrochaeris (Linnaeus, 1766) (Rodentia) em duas áreas urbanas no norte do Paraná, Brasil

This study aimed to evaluate the population ecology of Hydrochoerus hydrochaeris (Linnaeus, 1766) in two urban areas in the north of Paraná, an open and other protected by assessing the effects of these two different types of environments on the structure and dynamics of the population and its implications for conservation of the species. The monitoring of the populations were performed monthly between June 2014 and March 2015 in Jaboti Lake Park (Apucarana / PR) and Conservation Unit Parque Arthur Thomas (Londrina / PR). To conduct the survey population data was used the methodology of total census (direct counting of individuals).They were evaluated ecological parameters of the populations of capybaras, such as ecological density, abundance, age distribution and birth rate, as well as performed the analysis of the landscape. It was applied to analysis of variance (ANOVA) to test the differences between the averages of the abundance of each age group per year of observation, as well as the differences between the average ecological density over the years of observation. The standard relationship between the use of classes and land use in the landscape and the ecological parameters of the capybara populations was evaluated by Principal Component Analysis (PCA). The trend of variation of average abundance over time to Thomas Arthur Park revealed abrupt decrease of the population in a short period of time (2014 16 ± 9, 14 ± 1 and 2015 7±1) as well as ecological density in 2015 (0.05 ind./ha). On the other hand, the Jaboti Lake Park, showed an increase in absolute abundance, with marked recovery of the population in the same period (2014 38±8,30 and 2015 45±1,73) and coming up with an ecological density in 2015 (2 ind ./ha). The birth rate found Lake Park Jaboti was superior to Arthur Thomas Park, which had a negative rate between 2012 and 2015. The use and land cover analysis showed significant differences from the point of view of the relative contribution of landscape elements in the spatial heterogeneity. Arthur Thomas Park shows areas of dense vegetation and urban areas relatively higher than those observed in Jaboti Lake Park that revealed relatively higher proportions of the areas of agriculture / field and exposed soil. Thus, the present study revealed that the local population structure is directly related to the spatial characteristics of both studied landscapes, as can be seen by the greater abundance and density seen in Jaboti Lake Park compared to Arthur Thomas Park in recent years of study.

Caracterização de frutos, divergência genética e estrutura espacial de jaboticabeiras nativas de fragmento florestal em Clevelândia - PR

To design strategies for the conservation and use of genetic resources of tree species such as jaboticaba tree, it is essential to make the characterization. In southwestern Paraná region, there are several forest fragments containing native jaboticaba tree (Plinia cauliflora), whose materials have broad potential for commercial orchards or breeding programs. As is the potential genetic diversity of a population to produce different genotypes, it would be able to start in such a characterization one of these fragments. The aim was to characterize fruits of jaboticaba tree (P. caulifora) of forest fragment kept in Clevelândia - PR for the presence of phenotypic variability, seeking to identify those superiors named for future selection as farming or male parent, as well as estimate genetic divergence between them, as a complementary tool for this purpose. Also, verify the regeneration and spatial distribution of the species. For the study was defined portion of a hectare (10.000 m²), with all individuals identified, mapped, with local coordinate system, and measured height and diameter. Fruits were characterized by sensory and biochemical characteristics in two years, 70 genotypes at 2013 and 56 at 2014, and of these 33 genotypes in both years. As a pre-selection criteria was adopted the choice of 20% of the genotypes that showed the highest frequency of superiority in the evaluated characteristics of the fruit. Genetic divergence among 33 genotypes per year was analyzed. The distribution pattern and spatial association was evaluated by Ripley's K function. It was classified for the first time the following ontogenetic stages of jaboticaba tree, by plant height, seedling (from 0.01 to 0.99 m), juvenile (1.0 to 4.99 m), immature (> 5.0 m, non-reproductive), adult (reproductive). It was also have been describe for the first time the naturally occurring juxtaposed seedlings, indicating polyembryony. The number of regenerating identified in the population (seedlings: n = 2163; juveniles: n = 330; immature: n = 59) was much larger than the number of adults (n = 132). The species showed reverse J-shaped size structure standard, with high concentration of regenerating. The regeneration distribution occurs in aggregate pattern and there is seedling-adult dependence, due seed dispersal and seedling emergence closest to mothers. The jaboticaba tree regeneration is sufficient to maintain the species for long term in this population, which should serve as reference to regeneration success for other studies of this important fruiting species from Ombrofile Mixed Forests. Has been pre-selected the jaboticaba trees 7, 42, 43, 47, 54, 91, 97, 104, 105, 118, 134, 153, 154, 157, 163, 169, 177, 186, 212, J7-01 and J7- 02, and 16 and 194 the ones that can now be selected by the superior characteristics of both cycles. It was recommended to carry out hybridization between genotypes 79 and 119, and 96 to 148. The quality of fruit analyzed showed potential for use as a dual purpose serving both in natura market or processing.

Reator de leito estruturado com recirculação submetido à aeração intermitente no tratamento de esgoto sanitário

The release of nitrogen compounds in water bodies can result in many environmental problems, so treat wastewater, such as sewage in order to remove not only organic matter but also nitrogen has been studied a few decades. From the above, the objective of this study was to evaluate the performance of a structured bed reactor, continuous flow, with recirculation, in removing organic matter and nitrogen present in wastewater under different cycles of intermittent aeration (AI) and to evaluate the influence of these cycles in the development of nitrifying bacteria (Oxidizing Bacteria Ammonia - BOA and Bacteria Oxidizing Nitrite - BON) and denitrifying (DESN) adhered (Support Material - MS) and suspension (Effluent - EF and sludge - LD). The reactor used has usable volume of 9.4 L. As support materials (MS) polyurethane foam was used, cut and fixed in PVC rods. 3 were worked aeration phases (AE) and non-aeration (AN) at different stage: Stage 1 (4 h EA / AN 2H); Stage 2 (2H EA / AN 1 h) and Phase 3 (2H EA / AN 2 h). During all hydraulic detention time phases was kept at 16 h and the effluent recirculated at a rate of 3 times the inflow. Were analyzed: pH, total alkalinity, temperature, chemical oxygen demand (COD), Biochemical Oxygen Demand (BOD), nitrogen Kjeldhl Total (NKT), ammonia-N-N-NH4+, nitrito-N-NO2+andnitrato-NO3-. The concentration of BOA, BON and DESN was determined using the number More Provável.gSSV-1 (NMP.gSSV-1). In phase 1 the percentage removal NTK N-NH4+ and NT was 76±10%, 70±21% and 67±10% respectively. In Phase 2 80±15% of removel NKT, 86±15% of N-NH4+ e 68±9% of removel NT e na Fase 3 de 58±20%, 72±28% and 41±6% of NKT, N-NH4+ of NT, respectively. The denitrification efficiency in stage 3 was over 70%, indicating that occurred in the reactor the process of simultaneous nitrification and denitrification (NDS). DQOT the removal percentages were 88 ± 4% in Phase 1, 94 ± 7 in Phase 2 and 90± 11% in Phase 3. The multivariate ANOVA applied to NMP.gSSV-1, it indicated that there was significant (F: 20,2, p <0,01) between the analyzed concentration of organisms AI in different cycles, but the differences between NMP.gSSV-1 depends not only isolated factors but of which means, and phase groups being analysis. From the results it is concluded that the working system is efficient in terms of nitrogen removal and organic matter, and that the stage with the highest availability of Dissolved Oxygen (DO) and C/N ratio (Step 2), was the one obtained the lower concentrations of organic matter effluents and N-NH4+. Hinted that there was a significant difference between the concentration (NMP.100mL-1) of the analyzed organizations (BOA, BON and DESN), but this difference does not depend on factors alone but of which means (MS, EF or LD), stages (1, 2 or 3) and groups (BOA, BON and DESN) is being considered.

Rede de conservação on farm para fruteiras nativas da fitofisionomia floresta com araucária: levantamento de informações

Currently, the biodiversity is considered as a powerful food security strategy, ecological and economical for humanity. Brazil is one of the main centers of genetic diversity of native fruit in the world. However, little is known about most of these species. In southwestern Paraná region, this diversity can be found, however, due to human action to increase genetic erosion, it is losing genotypes with potential for use. Thus, the conservation of genetic resources is essential for reduction strategies for damage caused to the environment and the lack of tech-nical information to increase the use of them. This study aimed to obtain information for cre-ating on farm net conservation in four citties this region. This study was carried out in rural properties from Dois Vizinhos, Itapejara D’Oeste, Verê and São Jorge D'Oeste citties, Paraná State, Brazil. It was action plan was established with the rural communities through gathering information with agents considered key in the process, it seeking the greatest number of farm-ers who had their properties in the native fruits as pitanga, jabuticaba, uvaia, cereja-do-mato, guabiroba, guabiju, sete capote, goiaba serrana, araça amarelo e vermelho trees. Semi-structured questionnaire was applied, which concerned issues of presence, handling and use of Myrtaceae fruit trees on their properties and informed consent term. There was a survey of the number and native fruits present in each property. The characterization of each household in terms of diversity handled and used in native fruit was performed. It was realized the soil col-lect in 200 properties with the presence of at least some native fruit tree naturally occurring, in order to determine the preference of the species for the chemical characteristics of the soil. The four citties have native fruits trees in quantity and diversity for the creation of on farm net conservation, with farmers demonstrating knowledge of their role as guardians of this heritage of humanity.

Altura do pasto e adubação nitrogenada sobre a produção de forragem e eficiência no uso de nutrientes em sistema de integração lavoura-pecuária

The proper use of management strategies, such as grazing intensity and nitrogen fertilization are primordial to the success of integrated crop-livestock system. Several studies have demonstrated the influence of grazing intensity and nitrogen fertilization on dynamics of forage production and nutrient cycling. However, most this researches studying these strategies in isolation and little is known about the interaction of these factors in the management of an integrated crop-livestock system. In this context, the aim of this study is to determine the best management strategy involving sward height and nitrogen fertilization, permitting greater forage production and improved efficiency in the use of nitrogen soil by a black oat ‘BRS 139’ plus ryegrass ‘Barjumbo’ pasture in integrated crop-livestock system. The experiment was realized in Abelardo Luz – SC, in an area of 14 ha, where has been conducted an experiment in long term with integrated crop-livestock system under no-tillage since 2012. The experimental design is a randomized block design with three replications in a factorial design (2x2), the first factor was the grazing intensity (high and low), characterized by two sward height management (10 and 25 cm), and the second included the time factor application of N in the system: N applied on pasture (N-pasture) and N applied on the culture of grain (N-grain), at dose of 200 kg N ha stocking and variable stocking rate. The previous crop to pasture was corn. The nitrogen fertilization of pasture increased tiller density, forage density, participation of ryegrass ‘Barjumbo’ and percentage of ryegrass leaves in forage mass. Forage mass was less at low sward height on average, however the percentage of ryegrass ‘Barjumbo’ and rye leaves was greater and dead material was lower in this treatment. With nitrogen fertilization of pasture it was possible to double the amount of forage accumulated in periods with further development of ryegrass, furthermore, the total production of DM was increased in 38.4% and the shoot N concentration in 28.6%. When the nitrogen fertilization is applied in pasture, it is possible to keep black oat ‘BRS 139’ plus ryegrass ‘Barjumbo’ pasture with an average sward height of 11 cm. The residual effect of N applied at corn was not sufficient to meet the nutritional needs of pasture and the forage production was affected by periods with N deficiency, while a single application of 200 kg N ha was sufficient to meet the N requirements throughout the forage accumulation period. The black oat ‘BRS 139’ plus ryegrass ‘Barjumbo’ pasture is efficient in use and recovery of the nitrogen applied in both treatments of sward height.

Enraizamento de mini-estacas de jabuticabeiras, pitangueira, araçazeiro amarelo e sete capoteiro

The most native fruit trees are belonging to Myrtaceae family, which it have as main marketing potential their fruit. Despite the wide acceptance of the fruits of these native fruit cultura, the establishment of commercial orchards is still necessary, because if it prevails extraction in the forest. To start the cultivo in the orchard, the first point is on the mother plant choice, which should provide superior characteristics when compared to other genotypes. Then, it is necessary to choose the method to can produce satisfactory amount of seedlings and preferably without it to lose the mother plant characteristics. For this, it adopts the asexual thechniques, with option for grafting, cuttings and air layering. These techniques when tested with native fruits tree, it proved limiting in theses results, with this, it should to test other it to recommend its use, especially, those fruit native of higher potential as jabuticaba tree, pitanga tree, sete capote tree and araça amarelo tree. The aim of this study was to test the use of asexual propagation through mini-cuttings in these native fruit trees, according to the time of collection, the mini-cutting length and concentration of IBA, as well as, it to relate the results of rooting with tryptophan extracted at certain times. The work was carried out at Universidade Tecnológica Federal do Paraná – Câmpus Dois Vizinhos, Brazil. The samples were collected each two months. The mini-cutting were prepared with 6 or 8 cm, with a pair of leaves reduced to 25% of the original size. The mini-cuttings had their base immersed in liquid solution of indole-butyric acid (IBA) in the concentrations of 0, 3000 and 6000 mg L-1 and then were placed in tubes containing commercial substrate. The experimental design was completely randomized with factorial 2 x 3 x 6 (mini-cutting length x IBA concentration x time of collection), with four replications, it being each plot varied according to the amount of shoots obtained by period time. After 120 days, the rooting and callus formation (%), average number of roots per mini-cutting and the average length of the roots were evaluated. After 60 days of these evaluations, the survival of mini-cuttings rooted after transplant was evaluated. It was evaluated also the production of mini-cuttings of each size in each period time. At the end of the experiment it was evaluated the percentage of survival of mother plantlets. For analysis of tryptophan was used materials branches, leaves and twigs with leaves, taken from the materials used for the production of mini-cutting. It was recommended for hybrid jabuticaba tree the use mini-cutting with eight cm, treated with 6000 mg L-1 of IBA and collected in June. For jabuticaba tree of cabinho and araça amarelo tree the period for propagation by mini-cuttings should be in August, regardless of IBA concentration and length of the mini-cutting. In the jabuticaba tree sabara and sete capote tree is important to obtain more satisfactory results realized the collect in October or December, with the same independence of other levels tested in other factors. However, for sete capote tree should test other techniques to increase the efficiency of propagation. And with pitanga tree recommended to the collection in June, but with 6cm the application of 3000 mg L-1 of IBA and 8 cm with 6000 mg L-1 of IBA.

Herança genética e marcadores moleculares associados à resistência de Euphorbia heterophylla L. aos herbicidas inibidores da ALS e PROTOX

This study aimed to assess the genetic inheritance, determine the better DNA isolation protocol for this species and to identify molecular markers associated with the Wild Poinsettia (Euphorbia heterophylla L.) resistance ALS- and PROTOX- inhibiting herbicides and. The genetic inheritance of resistance was determined from crosses between E. heterophylla biotypes susceptible (S) and resistant (R), backcrosses and F2 generation. The complete dominance of resistance was confirmed with dose response curves. Ten adjusted methods for DNA isolation described in the literature were tested. The specific primers for ALS and PROTOX genes were designed from the consensus DNA sequence of these genes, obtained by aligning the gene sequences of the species Manihot esculenta and Ricinus communis L. Additionally, it was assessed the transferability of twenty SSR (simple sequence repeat) markers designed for Manihot esculenta, because among the species of Euphorbiaceae with more developed SSRs markers, because it is the closest relative phylogenetic species of E. heterophylla. Regarding genetic inheritance, the frequencies observed in the F1, F2, RCs and RCr did not differ significantly from the expected frequencies for a trait controlled by two dominant genes for multiple resistance and a single dominant gene for simple resistance to ALS- and PROTOX-inhibiting herbicides. The similar levels of resistance to dosage up to 2000 g i.a. ha-1 of fomesafen and dosage up to 800 g i.a. ha-1 of imazethapyr observed in F1 (heterozygous) and homozygous R biotype confirm the complete dominance of resistance to PROTOX- and ALS-inhibiting herbicides, respectively. The 0.2%BME protocol allowed the isolation of 7,083 ng μL-1 DNA, significantly (P=0.05) higher than other methods. Co-isolation of phenolic compounds was observed in FENOL and 3%BME+TB methods, but the addition of polyvinylpyrrolidone (PVP40) in the protocol extraction buffer 3%BME+TA solved this problem. The primers designed for ALS and PROTOX genes amplified but not showed no visible polymorphism in agarose gel between the S and R biotypes of E. heterophylla. Regarding the SSR transferability, ten markers were transferred to E. heterophylla, however, these six primers showed polymorphism among S and R biotypes.

Desempenho de soja associado à posição do fertilizante e tamanho de sementes

The seed size used for seeding has caused doubts among soybean producers. The study aimed to determine whether there may be differences between seed size with respect to depth of fertilizer deposition. The field experiment was conducted at the Experimental Area UTFPR Campus Pato Branco, using a precision seeder for direct seeding. The design was a randomized blocks, with five repetitions. The treatments were composed by the combination of two seed sizes (large seed with 6,5 mm and 5,5 mm with small seed) and two fertilizer deposition depths in relation to the seed (fertilizer near the seed with about 3 cm away and fertilizer distant from the seeds with about 10 cm). Data were subjected to analysis of variance. When the test value F was significant at 5% probability was applied to the Duncan test for comparison of means. The shallower depth of fertilizer deposition provided larger number of pods per plant and increased number of grains per plant. Already the largest depth of fertilizer deposition provided greater plant height at 30 days after sowing and R2 stage, greater ground area mobilized, higher plant population in all periods, greater depth of deposition of seeds and a higher rate of emergency speed.

Nitrogênio mineral em um latossolo em função de sistemas de manejo do solo e de culturas de cobertura de inverno

The increasing advancement of agriculture makes providing adequate conditions for the growth and development of plants is the primary purpose of soil management systems. Much of the success of PD is attributed to cultural remains left by cover crops that do not require high nitrogen inputs and can thus be used to reduce nitrogen input in the agro- ecosystem. The nitrogen is one of the elements applied in agriculture, it is absorbed in higher quantities and limiting the yield of grain crops such as corn. Thus, there has been the influence of the no-till and conventional tillage combined with different crops of winter cover and bare soil when in succession to corn, on mineral nitrogen content. The experimental work was made at the experimental station of the Agronomic Institute of Paraná - Iapar. The implemented design was blocks at random split plot with three replications in factorial 6 x 2 x 3 x 5. The main plots were as treatment, beyond the bare soil, 5 winter species (ryegrass, vetch, vetch + oat, oat and radish), while in the subplots were used two tillage systems (No-till and conventional tillage). Three collections made were (before management, the urea before and after the urea), these being held in 5 depths (0-5, 5-10, 10-20, 20-40 and 40-60 cm). So a layer 0-5 cm and a que presents increased amount to NH4 + ion. The use of associated PD system in the presence of winter cover crops decreased as NO3 - losses in soil profile.

Habilidade competitiva do feijoeiro e sua tolerância ao herbicida Ethoxysulfuron

The presence of weeds decreases the crop yield. Among the alternatives to reduce the crop yield loss, it can be included to increase the competitive ability of the crop and the chemical control of the weeds. A research program was developed in the course of Agronomy at Federal Technological University at Paraná, Campus Pato Branco - PR, during the years 2015/16, with the objectives evaluating if gibberellin inhibitors increase the competitive ability of bean plants, making them insensitive to the initialism, extending the period prior to weed-crop interference. Evaluate the tolerance of common bean plants to the herbicide ethoxysulfuron and investigate the existence of relationship between the plant mass and the level of tolerance of the plants to the herbicide. Evaluate the effect of increasing doses of ethoxysulfuron on morphological characteristics, yield components and grain yield of the bean cultivars IPR Tangará and IPR Andorinha. Evaluate the effect of increasing doses of ethoxysulfuron on the development of IAC Imperador and the community of weeds present in the area. Elucidate the mechanism that confers tolerance to bean plants to the herbicide ethoxysulfuron. The results indicate that gibberellin inhibitors were not effective in increasing periods of weed-crop coexistence. Trinexapac-ethyl increased 20% the grain yield of bean plants. It was observed high variability as the response of bean cultivars to the herbicide ethoxysulfuron, however, despite high doses (200 g ha-1), it was not observed death of the plants. The field results indicate that when the ethoxysulfuron dose is 83.2 g ha-1, the reduction in grain yield can reach 40% with the cultivar IPR Tangará and 30% in the cultivar IPR Andorinha. However, respectively for each cultivar cited, ethoxysulfuron at 17 and 12 g ha-1 are enough to reduce 10% of grain yield. Evaluating the control of weeds within the bean crop cultivar IAC Imperador with the herbicide ethoxysulfuron, it was observed that doses at 20 g ha-1 are enough to control soybean and Ipomoea spp. plants. But, due to the level of plant injury, the crop grain yield increase was not sufficient to match the one observed on the weed-free untreated control. The mechanism of tolerance of bean plants to ethoxysulfuron appears to be the herbicide degradation.