Qualidade dos estudos clínicos publicados na RBGO ao longo de uma década (1999-2009): aspectos metodológicos, éticos e procedimentos estatísticos

OBJETIVO: Avaliar a evolução metodológica e do delineamento estatístico nas publicações da Revista Brasileira de Ginecologia e Obstetrícia (RBGO) a partir da resolução 196/96. MÉTODOS: Uma revisão de 133 artigos publicados nos anos de 1999 (65) e 2009 (68) foi realizada por dois revisores independentes com formação em epidemiologia clínica e metodologia da pesquisa científica. Foram incluídos todos os artigos clínicos originais, séries e relatos de casos, sendo excluídos os editoriais, as cartas ao editor, os artigos de revisão sistemática, os trabalhos experimentais, artigos de opinião, além dos resumos de teses e dissertações. Características relacionadas com a qualidade metodológica dos estudos foram analisadas por artigo, por meio de check-list que avaliou dois critérios: aspectos metodológicos e procedimentos estatísticos. Utilizou-se a estatística descritiva e o teste do χ2 para comparação entre os anos. RESULTADOS: Observa-se que houve diferença entre os anos de 1999 e 2009 no tocante ao desenho dos estudos e ao delineamento estatístico, demonstrando maior rigor nos respectivos procedimentos com o uso de testes mais robustos, relativamente, entre os anos de 1999 e 2009. CONCLUSÕES: Na RBGO, observou-se evolução metodológica dos artigos publicados entre os anos de 1999 e 2009 e aprofundamento nas análises estatísticas com o uso de testes mais sofisticados, como o uso mais frequente das análises de regressão e da análise multinível, que são técnicas primordiais na produção do conhecimento e planejamento de intervenções em saúde. Isso pode resultar em menos erros de interpretações.

Consumo e digestibilidade de dietas contendo fontes energéticas associadas ao farelo de girassol ou ureia em novilhos confinados

Objetivou-se avaliar o consumo e a digestibilidade total e parcial de dietas utilizando milho, casca de soja e polpa cítrica associados ao farelo de girassol ou ureia em novilhos confinados. Foram utilizados quatro novilhos ½ Aberdeen Angus vs. ½ Nelore, providos de cânulas no rúmen e duodeno, com 18 meses de idade e 360 kg de peso médio inicial. As dietas foram constituídas por duas fontes energéticas (milho grão e polpa cítrica) associadas a duas fontes nitrogenadas (ureia e farelo de girassol), totalizando quatro tratamentos, numa proporção volumoso:concentrado de 40:60. O delineamento utilizado foi o quadrado latino com quatro tratamentos e quatro períodos de 21 dias cada. O consumo médio diário de matéria seca foi influenciado (p < 0,05) entre as dietas, e o maior valor foi atribuído à dieta milho e farelo de girassol. A digestibilidade aparente de todos os componentes, exceto da proteína bruta, foi maior (p < 0,05) para as dietas com polpa cítrica. Não houve efeito (p > 0,05) entre as dietas para as digestibilidades ruminal e intestinal. A utilização de coprodutos na alimentação animal não prejudicou a digestibilidade das dietas, sendo indicados como ingredientes de elevado potencial de utilização em dietas para bovinos de corte.

Metabolismo da videira ‘syrah’ no semiárido nordestino sob três estratégias hídricas

The almost complete absence or misdistribution of water as a natural resource frequently constitute a limiting factor for plant growth and development in the semi-arid northeastern Brazil. In this context, the use of appropriate irrigation techniques is an essential and indispensable factor for proper functioning of the primary and secondary metabolisms in plants. This study aimed to assess the metabolism of the Syrah grapevine in the semi-arid northeastern Brazil, by using three irrigation strategies (controlled deficit irrigation [CDI], deficit irrigation [DI], and full irrigation [FI]). The research was conducted at Embrapa Semiarid (Embrapa-Brazilian Agricultural Research Corporation), Experimental Field of Bebedouro, municipality of Petrolina, state of Pernambuco, Brazil. The statistical design was randomized blocks, with a 3 x 6 factorial scheme. Six samplings were performed throughout the experiment cycle at 54, 60, 73, 87, 101, and 115 days after pruning (DAP). We analyzed the total soluble sugars, reducing sugars, total soluble protein, and invertase activity. Analysis of variance and the F test were performed for all analyzed variables. The means were compared using the Tukey test at 5% significance. At the end of the experiment cycle, DI was found to increase the average acid invertase activity in the plant cell wall. Further, by the end of the experiment cycle, the total soluble sugars and reducing sugars increased in all plants in the three irrigation strategies. Thus, we conclude that it is possible to reduce water consumption in vineyards of the semi-arid northeastern Brazil, without significantly affecting the protein and sugar metabolisms in the plants.

Adubação nitrogenada na cultura do milho com ureia revestida por diferentes fontes de polímeros

In view of theoretic increase in efficiency of nitrogen fertilizers for controlled release, this study aimed to evaluate the effect of nitrogen, using conventional urea and ureas coated by different polymers, in the leaf N content, leaf chlorophyll index, components production and grain yield of irrigated corn in growing season and second crop in the savannah region. The experiments were conducted at experimental area belonging to UNESP - Ilha Solteira, located in Selviria - MS in a dystrophic Red Latosol (Haplustox), clayey texture. The statistical design was randomized blocks, with four repetitions, in a 4 x 4 factorial arrangement, being four nitrogen doses (0, 40, 80 and 120 kg ha(-1)) applied at sidedressing and four urea sources (a conventional urea and three coated with polymers in different compositions and concentrations). The coated ureas are not efficient under the soil and climate conditions studied of the savanna, because they provided results similar to the conventional urea for the production components and grain yield of corn in the first and second crop. The increment of nitrogen doses increase linearly the leaf N content and grain yield of corn in the first and second crop.

Estimativas de parâmetros genéticos para seleção precoce de clones de Eucalyptus para região com ocorrência de geadas

Further to increase the wood yield, is important goal of forest tree breeding the adaptation of clones through diverse environment, especially for soil and climate. Perennial tree species such as eucalypts, have a long life cycle and the frosts can occur and to be their limiting source of cultivation. The aim of the study was to determine the genetic variability and the genetic correlations between selection ages of clones cultivated in the municipality of Palma So la, SC, Brazil, where frosts are common in the winter. A clonal trial was set up in 2008 in Palma So la, by statistical design of randomized complete blocks considering 29 clones, six replications, six plants per plot, and 3.0m x 3.0 m spacing. The silvicultural characters of total plant height, diameter of breast height (dbh), and wood volume were evaluated through 24, 36, 48, and 60 months old. The deviance analysis and estimates of genetic parameters were based on the REML / BLUP genetic statistical procedure. Significant differences were observed for all traits in the deviance analysis. High correlations and statistically significant between characters were observed, indicating that the early selection will provide significant gains. Part of the clones USP/IPEF 64, USP/IPEF 78, USP/IPEF 52, USP/IPEF 68, and USP/IPEF 74) are potential to be used into the breeding programs and in commercial stands into the studied frost regions.

Influência do uso de malhas de sombreamento azul, vermelha e preta no desenvolvimento inicial do pepino Taiko

Cucumber (Cucumis sativus L.) is a plant of the Cucurbitaceae family of great commercial importance and their culture is widespread throughout the country. The production of vegetables, high-quality care begins with training ranging from seedlings and shading techniques have an important role in creating environments more conducive to the production of seedlings and many researchers and producers of seedlings have opted for the use of color shading screens, making this way, the wavelength and the amount of incident energy are changed in favor of the plants. Work will be performed at the Department of Forest Sciences, Faculty of Agricultural Sciences, Universidade Estadual Paulista (UNESP), Botucatu campus. The statistical design will be in three blocks, each containing 100 plots of each treatment (seedlings covered with blue screen, red and black). Evaluations of dry mass and height will be made at 7, 14, 21 and 28 days after emergence, and 5 will be selected seedlings of each treatment. At 14 and 28 DAE was measured, and SPAD chlorophyll content at 30 DAE, was evaluated by measuring gas exchange in the second pair of leaves, fully expanded for each plant, which was put in a chamber controlled with infrared gas analyzer (IRGA LI 6400, Licor) to perform measurements of photosynthesis. Treatment with black shading showed statistically better results for total dry matter, plant height, SPAD chlorophyll content and net assimilation of CO2, showing a better quality seedlings and its use is recommended in the cultivation of cucumber Taiko

Influência do desponte de panículas de sorgo sacarino sobre a qualidade da matéria-prima e produção de bioetanol

Pós-graduação em Microbiologia Agropecuária - FCAV

Balanço hídrico, crescimento e produtividade de genótipos RB de cana-de-açúcar em cultivo de sequeiro na região de Rio Largo-AL

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Utilização de resíduos de tratamento de esgoto como suprimento hídrico e nutricional na cultura do girassol

Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

Interferência de plantas daninhas no desenvolvimento inicial de mudas meristemáticas de cana-de-açúcar

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Acúmulo de massa seca na soja em resposta a aplicação foliar com silício sob condições de déficit hídrico

The foliar fertilization with silicon has promoted several actions beneficial to plants, among them is greater drought tolerance, however, for the soybean, there is little information on these benefits in this condition. Thus, the aim of this study was to evaluate the effect of silicon on leaf, the dry matter accumulation of soybean in their reproductive stages, where the crop water stress suffered during the same. The experiment was carried out at the Plant Science Unit Aquidauana University - State University of Mato Grosso do Sul. The statistical design was a randomized block split plot with four replications. The plots were represented by cultivar 5DR615, the subplots consisted of the application (with or without) silicon, whose source was used KSi. Was measured the height and identified the development stage of all plants, separating them into stem + branches, leaves + petioles, pods capsules and seed. Foliar applications of silicon increased dry matter accumulation during the reproductive stage of soybean, where the highest values occurred in the R6 stage. Under conditions of water deficit, foliar application of silicon on soybean provided normal plant development, generating greater dry mass of stem + branches, leaves + petioles, pods capsules and seeds throughout their reproductive phase, with the highest values obtained at R6 stage (35 days after R2).

Effects of different coagulants on sweet sorghum juice clarification

Sweet sorghum is a potential complementary crop for ethanol production in Brazil, United States and India. Since, phenols are playing inhibitory role in the process of ethanol production from extracted juice from sweet sorghum stalks, it's removal from juice is necessary for better ethanol yield. The aim of this research was to evaluate the use of magnesium oxide and calcium hydroxide as coagulant agents in different pH levels in the juice treatment. The experiment was arranged in a split-plot statistical design. Main treatments corresponded to the different coagulants and secondary treatments were the pH levels (6.0 and 7.0). Analysis of coagulant volume, juice pH, settling rate and sludge volume were performed during the decantation process. Clarified juice was analyzed as Brix, pH, ashes, phenolic compounds and starch. The use of magnesium oxide resulted in lower sludge volume, phenolic compounds and ashes. The use of calcium hydroxide decreased starch content, whereas clarification at pH 7.0 promoted higher sludge volume. Treating juice with magnesium oxide improved decantation dynamics of the process and clarified juice quality.

Qualidade de cebola minimamente processada em diferentes tipos de cortes

The high perishability of the onion has limited its conservation period and caused great post-harvest losses. The need of consuming fresh products has led the market of minimally processed products to considerably rise. Cut operations during the minimal processing may not only cause important biochemical reactions in the onion tissues, but also affect its sensorial quality, resulting in a drastic reduction in the post-harvest life of the product. In this study the influence of different cut types was assessed on the quality of minimally processed onions. 'Baia Periforme'onions were transported to the laboratory, where they were selected, cleaned, classified, peeled, minimally processed into slices, grated and chopped, and subsequently sanitized for 10 minutes in sodium hypochlorite at 100 mg L-1. The product was kept on polystyrene trays covered with plastic film (PVC) as 80 g portions and conserved in a cold chamber at 5 +/- 1 degrees C and 85 +/- 5% RH, during 6 days. Physical, chemical and physico-chemical assays were carried out. Statistical design was completely randomized with 3 treatments and 3 replications (plot group) and 5 replicationss for the control group. For sensorial analysis (aspect and smell), 10 replications were used. To compare means, Tukey test at 5% significance was adopted. During the refrigerated conservation, sliced onions kept more stable levels of soluble solids, titratable acidity, total sugars, reducing and non-reducing sugars. The grated onion was superior considering the sensorial characteristics, indicating that the cut types interfered in these features and the grated onion was more accepted by evaluators.

Resposta funcional da joaninha Cryptolaemus predando Cochonilha branca em diferentes temperaturas e substratos vegetais

This work was carried out to evaluate the functional response of Cryptolaemus montrouzieri Mulsant, 1850 (Coleoptera: Coccinellidae) fed with Planococcus citri Risso, 1813 (Hemiptera: Pseudococcidae) reared on a pumpkin hybrid (Cucurbita maxima x Cucurbita moscata) (Cucurbitaceae), seedlings of Rangpur lime (Citrus limonia) Rutaceae) and potato (Solanum tuberosum) (Solanaceae) at two temperatures. The predation rate of C. montrouzieri was measured using Petri dishes of 15 cm diameter with 1, 2, 4, 8, 16 and 24 adults of P. Citri. One third instar larva, one fourfh instar and one newly emerged adult (without differentiation of sex) of C. montrouzieri were added to each plate. The study was conducted in climatic chambers at temperatures of 25 and 30 degrees C and photophase of 12 hours. The predation rate was evaluated after 24 hours of prey exposition to the predator, by counting the number of preys trapped in the different treatments and control. The statistical design was completely randomized with four treatments x 6 subplots with 7 repetitions, the two temperatures. The values obtained were subjected to analysis of variance, to relate the number of scales preyed by larvae and adults of C. montrouzieri set up in different substrates. The amount of prey consumed by larvae and adults of the predator increased with increasing the prey density until it reaches a plateau, characterizing functional response type II. In general, the number of scales preyed by larvae and adults of C. montrouzieri was higher on potato and under temperature of 30 degrees C.

Moringa extracts used in sugarcane juice treatment and effects on ethanolic fermentation

The objective of this study was to evaluate the effects of sugarcane juice treatment using Moringa oleifera leaf and seeds extracts on ethanolic fermentation. The experiment was arranged in a split plot statistical design, with four replications. Main treatments were three sedimentation agents (synthetic polyelectrolyte, moringa leaf and seed extracts) and control while the secondary treatments were two sugarcane varieties (RB867515 and CTC4). Extracted sugarcane juice was clarified by simple defecation with pH adjusted to 6.0. The flocculating agents were added in a decanter before the limed juice. After then, the juice was standardized to 16° Brix at pH 4.5, and musts were inoculated with yeast Saccharomyces cerevisiae strain, FT858. At the end of the fermentation process, wines were recovered by centrifugation. In all experimental stages, extracted juice, clarified juice and wine were chemically and technologically characterized. The use of moringa leaf and seed extracts as sedimentation adjuvants did not increase the sedimentation speed of impurities. However, there was a high sludge compaction, which was essential for maintenance of yeast and bud population at the beginning of fermentation, and yeast budding rate in the end. The use of different sedimentation agents as adjuvants in juice treatment did not affect wine quality and ethanol yield.