The impact of varying nutrient regimes in earthen freshwater ponds on the comparative ecology of diurnally occurring candidate live feeds

The daytime composition and relative abundance of zooplankton species were studied in three treatments of two replicate earthen ponds each with nutrient sources and water replenishment regimes. Treatment -A (200m super(2) surface area supplied 900kgha super(-1) pig manure only). Treatment -B (200m super(2) surface area supplied 70kgha super(-1) month super(-1) pig manure, 50kgha super(-1) month super(-1) N.P.K. [15:15:15] and 30kgha super(-1) month super(-1) Urea) and Treatment-C (1500m2 surface area supplied 1150kgha super(-1) month super(-1) commercial grade 40% crude protein compounded feed). Water replenishment for Treatment A was daily tidal deluge from the New Calabar River while that for treatment B and C was from column-well and occasional rains. No zooplankton species were recovered from the pig-manure only treatment (A) while only Diffugia constricta and Difflugia urceolata were the two protozoans that occurred together in treatments B (combined fertilization) and C (compounded feed only) in contrast, Difflugia acuminate and three rotifers, Collurella uncinata, Diurella stylata and Keratella quadrata occurred only treatment B. similarly, Arcella arenaria, Arcella costata, Centropyxis aculeate, Difflugia pyriformis, Branchionus calyciflorus, Lepadella patella, Polyarthra trigla and Onchocanmptus mohammedi were recovered from treatment C. Arcella costata was the most abundant zooplankton in the entire experiment, while Arcella arenaria was very abundant in treatment C, Collurella uncinata was very abundant in treatment B. The inference is that combined fertilization of earthen freshwater ponds tend to be more suitable for the culture of rotifers such as Brachionus calyciflorus, popular in fish larva nursery, while those supplied compounded feed could be used to produce protozoans where desirable

Symposium on Llangrose Lake: the ecology and conservation of a large nutrient-rich lake in South Wales

This paper summarises a meeting which discussed the ecology and conservation of Llangorse Lake in South Wales. The meeting was organised by the British Ecological Society (Aquatic Ecology Group), in association with the Countryside Council for Wales (CCW), Brecon Beacon National Park Authority (BBNPA) and Environment Agency Wales. It took place on 22 October 1998.

Advisory report on nutrient levels and related ecology of Malham Tarn

Aspects of reported nutrient levels and their ecological implications for Malham Tarn are discussed. Discussion centres upon the data given here as appendices, involving possible evidence of a long-term increase in the concentrations of some nutrients (especially nitrate) of significance for the Tarn's ecology and conservation. Further comparative tests of some methods of chemical analysis employed in obtaining those data are reported.

I. Configurational stability and redistribution equilibria in organomagnesium compounds. II. Redistribution equilibria in organocadmium compounds

I. CONFIGURATIONAL STABILITY AND REDISTRIBUTION EQUILIBRIA IN ORGANOMAGNESIUM COMPOUNDS

The dependence of the rate of inversion of a dialkylmagnesium compound on the solvent has been studied.

Examination of the temperature dependence of the nuclear magnetic resonance spectrum of 1-phenyl-2-propylmagnesium bromide in diethyl ether solution indicates that inversion of configuration at the methylene group of this Grignard reagent occurs with an approximate rate of 2 sec^-1 at room temperature. This is the first example of a rapid inversion rate in a secondary Grignard reagent.

The rates of exchange of alkyl groups between dineopentylmagnesium and di-s-butylmagnesium, bis-(2-methylbutyl)-magnesium and bis-(4, 4-dimethyl-2-pentyl)-magnesium respectively in diethyl ether solution were found to be fast on the nmr time scale. However, the alkyl group exchange rate was found to be slow in a diethyl ether solution of dineopentylmagnesium and bis-(2-methylbutyl)-magnesium containing N, N, N', N'-tetramethylethylenediamine. The unsymmetrical species neopentyl-2-methylbutyl-magnesium was observed at room temperature in the nmr spectrum of the solution containing the diamine.

II. REDISTRIBUTION EQUILIBRIA IN ORGANOCADMIUM COMPOUNDS

The exchange of methyl groups in dimethylcadmium has been studied by nuclear magnetic resonance spectroscopy. Activation parameters for the methyl group exchange have been measured for a neat sample and for a solution in tetrahydrofuran. The exchange is faster in the basic solvent tetrahydrofuran relative to the neat sample and in tetrahydrofuran solution is retarded by the solvating agent N, N, N’, N’-tetramethylethylenediamine and greatly increased by cadmium bromide. The addition of methanol to a solution of dimethylcadmium in tetrahydrofuran appears to have very little effect on the rate of exchange. The exchange was found to proceed with retention of configuration. The rate-limiting step for the exchange of methyl groups in a basic solvent appears to be the dissociation of coordinating solvent from dimethylcadmium.

The equilibrium between methylcadmium bromide, dimethylcadmium and cadmium bromide in tetrahydrofuran solution has also been studied. At room temperature the interconversion of the species is very fast on the nmr time scale but at -100° distinct absorptions for methylcadmium bromide and imethylcadmium are observed.

The species ethylmethylcadmium has been observed in the nmr spectrum.

The rate of exchange of vinyl groups in a solution of divinylcadmium in tetrahydrofuran has been found to be fast on the nmr time scale.

Evaluation of the nutrient composition of some unconventional feedstuffs

A trial was carried out to evaluate the nutrient composition of some unconventional feedstuffs to ascertain their suitability for incorporation into fish feed. Pawpaw seed, mudskipper meal, water melon seed, almond kernel and groundnut husk were processed and analyzed for their proximate and mineral composition using internationally established procedure. The results obtained showed that the experimental feed stuffs had percentage crude protein that ranged from 7.38% to 55.85%. The highest level (55.85%) was found in mudskipper meal while the lowest (7.38%) was found in groundnut husk. The experimental feed stuffs had appreciable levels of calcium, potassium and phosphorus ranging from 0.29% to 1.87%, 0.17 to 1.26 and 0.06 to 0.97 respectively. Almond kernel can be used to replace soybean meal in aqua feed. Also, mudskipper meal can be used to replace fish meal in order to produce a cheaper fish feed; but it was recommended that feeding trials should be conducted to ascertain the inclusion level at which they can be effectively incorporated. The potentials of some of these feedstuffs in fish feed formulation seem high.

Anadromous fish as marine nutrient vectors

The tidal freshwater of Virginia supports anadromous herring (Alosa spp.) spawning runs in the spring; however, their importance as nutrient delivery vectors to the freshwater fish food web remains unknown. The stable isotope signatures of fishes from 21 species and four different guilds (predators, carnivores, generalists, and planktivores) were examined in this study to test the hypothesis that marine derived nutrients (MDNs) brought by anadromous fish would be traced into the guilds that incorporated them. Spawning anadromous fish were 13C and 34S-enriched (δ13C and δ34S of approximately 18‰ and 17.7‰, respectively) relative to resident freshwater fish. Of the guilds examined, only predators showed 13C and 34S-enrichment similar to the anadromous fish; however, some generalist catfish also showed enriched signatures. Specific fatty acid δ13C signatures for gizzard shad (Dorosoma cepedianum), blue catfish (Ictalurus furcatus), and alewife (Alosa pseudoharengus), show a 10‰ range among fishes, clearly reflecting isotopically distinct dietary sources. The δ13C and δ34S distribution and range among the freshwater fishes suggest that both autochthonous and allochthonous (terrestrial C3 photosynthetic production and MDN) nutrient sources are important to the tidal freshwater fish community.