LOSS OF THE SEX-CHROMOSOME IN HUMAN NEOPLASIAS OF THE NERVOUS-SYSTEM

Thirty-eight tumors (five grade I-II astrocytomas, three grade III astrocytomas, four glioblastomas, one oligodendroglioma, four ependymomas, one pineocytoma, three medulloblastomas, four acoustic nerve neurinomas, one intraspinal neurinoma, one neurofibroma, 10 meningiomas, and one craniopharyngioma) and three benign lesions of the nervous system were evaluated cytogenetically after in vitro culture. Sex chromosome loss was detected in 56% of the cases (-X in 13 of the 25 female patients and -Y in nine of the 16 male patients). The objective of the present report was to study the role of this abnormality in cells of the nervous system.

Immunohistochemical evaluation of Ki-67 and PCNA in canine mammary neoplasias: Correlation with prognostic factors and clinical outcome

E-cadherin is a cell-cell adhesion molecule and low e-cadherin expression is related to invasiveness and may indicate a bad prognosis in mammary neoplasms. The expression of cell proliferation markers PCNA and especially Ki-67, has also proved to have a strong prognostic value in this tumor class. The expression of these markers was related to the clinical-pathological characteristics of 73 surgically removed mammary tumors in female dogs by immunohistochemistry. There was no statistical correlation between these markers and death by neoplasm, survival time and disease-free interval. However, the loss of e-cadherin expression and marked Ki-67 expression (p=0.016) were considered statistically significant for the diagnosis (p=0.032). When evaluated as independent factors, there was evidence of the relationship between the loss of e-cadherin expression and high PCNA expression with changes in the body status (divided into obese, normal and cachectic) of female dogs (p=0.030); there was also evidence of the relationship between pseudopregnancy and e-cadherin alone (p=0.021) and for ulceration and PCNA alone (p=0.035). The significant correlation between the markers expression and these well known prognostic factors used individually or in combination suggests their prognostic value in canine mammary tumors.

Interleukin-8 as a prognostic serum marker in canine mammary gland neoplasias

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Sensibilidade do colo dental. Uma nova alternativa terapêutica.

The cervical hypersensitivity tooth was analysed and treated by application of a varnish with high fluoride ions content (Duraphat) and of a glass ionomer cement usually indicated to cavity lining (XR-ionomer-Kerr). This technique was applied in sixty-seven teeth of Clinic Graduation patients (Faculdade de Odontologia de Araçatuba) and demonstrated a high index of satisfactory results. The clinical findings of this study relate that, this technique is more one method of choose by clinicians.

Evolução no diagnóstico citológico do câncer de colo uterino - Novas tecnologias para o rastreamento citológico cervical

The cancer of the uterine cervix remains a serious public health problem in developing countries with low social-economic conditions. Papanicolaou smear has been used to screen women for cervical cancer since 1940, and it is widely recognized as an effective method for preventing cervical cancer. In Brazil, conventional Papanicolaou is the most common method used in diagnosis cytopathological routine, involving the morphological cell analysis by microscopic examination of cervical samples smeared on glass slides. On the other hand, the liquid cytology is an advantageous method whose specimen collected is put into a fluid alcohol based to guarantee the potential use of residual cellular material in cytobrush. Moreover, this method permits additional diagnostic studies, preserving the morphological cell features as the conventional one does. The aim of the present study was report the historical evolution of different cervical cytologic tests, emphasizing the advantages and disadvantages between conventional Papanicolaou method and new technologies in liquid based cytology.

Prótesis radífera: Alternativa en el tratamiento de neoplasias de cabeza y cuello

Radipherpus protheses are a means of treatment for malignant tumor-carrying patients. These protheses are used in cases of brachytherapy or external actinotherapy by contact and allow treating them with high radiation doses that are quickly released from the areas next to tumor, so that the radiations be restricted to the affected region, without the emissions damaging or destroying the soft tissues. Based on these considerations, the present paper was aimed at making literature review on the use of radipherous protheses for the treatment of head and neck neoplasias. © 2006 1995, Editorial Ciencias médicas.

Immunohistochemical evaluation of e-cadherin, Ki-67 and PCNA in canine mammary neoplasias: Correlation of prognostic factors and clinical outcome

E-cadherin is a cell-cell adhesion molecule and low e-cadherin expression is related to invasiveness and may indicate a bad prognosis in mammary neoplasms. The expression of cell proliferation markers PCNA and especially Ki-67, has also proved to have a strong prognostic value in this tumor class. The expression of these markers was related to the clinical-pathological characteristics of 73 surgically removed mammary tumors in female dogs by immunohistochemistry. There was no statistical correlation between these markers and death by neoplasm, survival time and disease-free interval. However, the loss of e-cadherin expression and marked Ki-67 expression (p=0.016) were considered statistically significant for the diagnosis (p=0.032). When evaluated as independent factors, there was evidence of the relationship between the loss of e-cadherin expression and high PCNA expression with changes in the body status (divided into obese, normal and cachectic) of female dogs (p=0.030); there was also evidence of the relationship between pseudopregnancy and e-cadherin alone (p=0.021) and for ulceration and PCNA alone (p=0.035). The significant correlation between the markers expression and these well known prognostic factors used individually or in combination suggests their prognostic value in canine mammary tumors.

Caracterização clínica, diagnóstico histopatológico e distribuição geográfica das neoplasias mamárias em cadelas de salvador, Bahia

Spontaneous tumors of the mammary gland in female dogs, represent the most frequent neoplasms observed in the small animal clinic, which explains the high interest of researchers in the study of this lesion. This study aimed to determine the frequency of mammary tumors in female dogs treated at HOSPMEV/UFBA, in Salvador, from January 2006 to April 2008, as well as to carry out the clinical characterization, histopathologic classification and the georeferencing of this pathology. The results showed a high prevalence of mammary tumors in female dogs, with most of the tumors in more advanced stages and the carcinomas in mixed tumors the predominant histologic type. There was maalso a.correlation between socioeconomic characteristics of the study population with known prognostic factors for mammary neoplasms in female dogs. These results reflect the delay in providing veterinary medical care. Thus, it's important to educate the population regarding the prevention and detection of mammary cancer in female dogs looking for an early diagnosis and appropriate therapy.

Caracterização imunoistoquímica de neoplasias de bexigas associadas à hematúria enzoótica bovina

Bovine enzootic hematuria is characterized by the development of hemangiomatous lesions from several types of neoplastic processes, from epithelial and mesenchymal origin. In this research the histogenesis of neoplastic lesions found in bladder of bovines with enzootic hematuria from Caparaó microregion in the state of Espírito Santo, Brazil was determined. To accomplish this objective, immunohistochemical analysis was performed with primary antibodies: anti-vimentin, anti-cytokeratin, anti-CD31 and anti-uroplakin. Neoplasms found included urothelial carcinoma, in situ carcinoma, adenocarcinoma, hemangioma, myxoma e hemangiosarcoma. Immunohistochemical staining of cytokeratin in epithelial neoplasms and vimentin in mesenchymal neoplasms was significant (p<0.05). CD31 was positive in all the vessels of all samples, however, the staining was significant (p<0.05) in the tumor endothelial cells of the vascular mesenchymal neoplasms, as in hemangiomas and hemangiosarcomas. Uroplakin III staining was uneven in several neoplastic types and showed no significant difference (p>0.05). Most neoplasms showed an atypical uroplakin staining on urothelium and, in the case of hemangiosarcomas there was no staining of the urothelium at all. The Spearman statistical analysis revealed a positive correlation (r= 0.63, p= 0.05) between CD31 and vimentin and between cytokeratin and uroplakin (rs= -0.61, p= 0.05). It was concluded that biomarkers anti-cytokeratin, anti-vimentin and anti-CD31 are important for the diagnosis of neoplasms epithelial, mesenchymal and vascular mesenchymal, respectively. It is possible to use vimentin and CD31 in association in vascular mesenchymal neoplasms and cytokeratin and uroplakin in epithelial neoplasms. The uroplakin is an effective marker, not only for tumor diagnosis, but also to evaluate the urothelial integrity.

Estado nutricional e níveis plasmáticos de trauma e seus precursores em pacientes portadores de neoplasias malignas de esôfago

Pós-graduação em Bases Gerais da Cirurgia - FMB

Immunodetecção de marcadores de prognóstico em neoplasias mamárias de gatas: correlação dos padrões classificados

Pós-graduação em Ciência Animal - FMVA