Untersuchungen uber Nitro-o-toluylendiamin.

Thesis (doctoral)--Universitat Marburg.

Untersuchungen über eine verbindung von nitro naphtochinon mit methylalkohol.

Thesis (Ph. D.)--Marburg.

Elektrochemische Reduktion einiger Nitro-Azo-Verbindungen /

Thesis (doctoral)--Grossherzoglich Hessische Ludwigs- Universitat zu Giessen, 1903.

Ueber die sulfurierung des o-nitro-m-kresols.

Thesis (doctoral)--

Beitrage zur kenntnis des o-p-dichlor-m-nitro-o-toluidins und s...

Thesis (doctoral)--

Zur kenntnis der hydrazone des salicylaldehyds und seiner nitro...

Thesis (doctoral)--

Uber Nitro-p-Toluyl-o-Benzoesaure und einige Derivate derselben /

Thesis (doctoral)--Universitat Greifswald, 1897.

Ueber o-Nitro-Phenyl-[alpha]-Picolyl-Alkin und einige seiner Derivate : Ueber Phenyl-[alpha]-Picolyl-Alkin /

Thesis (doctoral)--Konigl. Universitat Breslau, 1900.

Uber Jodoso- u. Jodoverbindungen aus Nitro-p.-Jodbenzoesaure /

Thesis (doctoral)--Universitat zu Heidelberg, 1894.

Ueber die einwirkung von anilin, p-toluidin, naphtylamin und o-, m-, p-amidobenzoësäure auf nitro naphtoehinon.

Thesis (Ph. D.)--Marburg.

Uber einige Nitro- und Nitroso-abkommlinge aus der Benzolreihe /

Thesis (doctoral)--Universitat Basel, 1900.

Uber (1)-phenyl-(3)-methyl-(4)-nitro-(5)-pyrazolon und (1)-phen...

Thesis (doctoral)--

Medicinal nitro compounds

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Alkaline nitro-benzene oxidation of model compounds and solid fuel derivatives

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Mapping nitro-tyrosine modifications in fibrinogen by mass spectrometry as a biomarker for inflammatory disease

There is a growing awareness that inflammatory diseases have an oxidative pathology, which can result in specific oxidation of amino acids within proteins. It is known that patients with inflammatory disease have higher levels of plasma protein nitro-tyrosine than healthy controls. Fibrinogen is an abundant plasma protein, highly susceptible to such oxidative modifications, and is therefore a potential marker for oxidative protein damage. The aim of this study was to map tyrosine nitration in fibrinogen under oxidative conditions and identify susceptible residues. Fibrinogen was oxidised with 0.25mM and 1mM SIN-1, a peroxynitrite generator, and methionine was used to quench excess oxidant in the samples. The carbonyl assay was used to confirm oxidation in the samples. The carbonyl levels were 2.3, 8.72 and 11.5nmol/mg protein in 0, 0.25mM and 1mM SIN-1 samples respectively. The samples were run on a SDS-PAGE gel and tryptically digested before analysis by HPLC MS-MS. All 3 chains of fibrinogen were observed for all treatment conditions. The overall sequence coverage for fibrinogen determined by Mascot was between 60-75%. The oxidised samples showed increases in oxidative modifications in both alpha and beta chains, commonly methionine sulfoxide and tyrosine nitration, correlating with increasing SIN-1 treatment. Tyrosines that were most susceptible were Tyr135 (tryptic peptide YLQEIYNSNNQK) and Tyr277 (tryptic peptide GGSTSYGTGSETESPR), but several other nitrated tyrosines were also identified with high confidence. Identification of these susceptible peptides will allow design of sequences-specific biomarkers of oxidative and nitrative damage to plasma protein in inflammatory conditions.