Efeitos do FK-506 na regeneração de nervos após neurorrafia láteo-terminal: estudo em ratos

Pós-graduação em Bases Gerais da Cirurgia - FMB

Os efeitos da manipulação do sistema proprioceptivo na locomoção de pessoas com doença de Parkinson com e sem congelamento da marcha: desenvolvimento, otimização e aplicabilidade de um sistema de vibração muscular

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos da terapia a laser associada ou não ao plasma rico em plaquetas na reparação do tecido muscular de ratos

Pós-graduação em Fisioterapia - FCT

Efeitos preventivos do treinamento físico na expressão das proteínas Cox-2 e Vegf de ratos tratados com dexametasona

Dexamethasone is a synthetic glucocorticoid widely used to treat allergic and inflammatory processes. This drug is used in three main situations, are used to contain acute or chronic inflammatory processes, or like immunosuppressive drug's. In these cases the patient will receive high doses for a chronic period and, therefore, has a much greater chance of adverse side effects, such as hypertension, diabetes and dyslipidemia. Dexamethasone promotes deleterious effects on the arachidonic acid pathway, when administered in high doses, because it is a potent anti-inflammatory drug. We recently demonstrated that dexamethasone significantly reduces the protein expression of vascular endothelial growth factor (VEGF) in both skeletal muscle and heart, but the mechanisms involved remain unclear. Meanwhile, exercise has been shown to be effective against high blood pressure, diabetes and dyslipidemia, promoting, among other factors, the increase in VEGF and angiogenesis. One possible explanation for these effects would be the creation of new vessels mediated by inflammation, or by the stimulation of the formation of products of the metabolism of arachidonic acid (AA), such as prostaglandin E2 (PGE2) and VEGF, by increasing the stimulation of the enzymes cyclooxygenase 1 and 2 (COX-1 and COX-2). Little is known about the preventive effects of training on the action of dexamethasone in the arachidonic acid pathway. Therefore, the aim of this study was to determine whether aerobic exercise training, performed before and concomitant treatment with dexamethasone, was able to prevent the effects of the dexamethasone in the protein expression of COX-2 and VEGF. For this, we used young Wistar rats (n = 40) which were randomly divided into 4 groups: sedentary control (SC), sedentary and treated with dexamethasone (SD), trained control (TC) and trained and treated with dexamethasone (TD). These rats performed aerobic exercise training, 60% of maximum capacity, 5

Efeitos preventivos do exercício físico sobre as proteínas ciclooxigenase-2 (COX-2) e fator de crescimento de vasos derivado do endotélio (VEGF) no músculo cardíaco em ratos tratados com dexametasona

Dexamethasone (DEXA) is a synthetic glucocorticoid widely used in the handling of several drugs, for its proven benefits in fighting inflammation and allergies. Despite their benefits, their chronic use leads to several side effects that include changes in the body in the metabolism of carbohydrates, lipids and proteins. Moreover, being an anti-inflammatory, acts on the arachidonic acid pathway, reducing the expression of the enzyme cyclooxygenase (COX-2) and growth factor derived from the endothelium of blood vessels (VEGF) in various tissues. However, its effects on the myocardium are still uncertain. The physical training (PT), in turn, promotes effects contrary to those caused by chronic use of DEXA, however, little is known about the preventive effects of TF in the side effects of Dexa in the myocardium. Therefore, the aim of this study was to determine if the TF has the ability to prevent and/or mitigate the effects of Dexa in protein expression of COX-2 and VEGF in the myocardium. Forty animals were divided into 4 groups: sedentary control (SC), sedentary treated with Dexa (SD), trained control (TC) and Trained treated with Dexa (TD) and submitted to a protocol of physical training on the treadmill for 70 days (1 h/day-5 days per week, 60% of physical capacity) or kept sedentary. Over the past 10 days, rats were treated with Dexa (Decadron, 0.5 mg/kg per day, ip) or saline. During training the animals were weighed weekly and during treatment daily. At the end of treatment was made to measure fasting glucose levels of animals. The rats were killed with excess anesthesia and cardiac muscle was removed, weighed, homogenized, centrifuged and stored at -20° C for analysis of protein expression of VEGF and COX-2 by Western blotting technique. Treatment with dexamethasone caused a weight loss of 18% in sedentary animals and 13% in trained as well as elevated levels of fasting glucose in sedentary (88%). The TF was unable to mitigate the loss in...

Efeitos preventivos do exercício físico na expressão da miostatina em ratos tratados com Dexametasona

Muscle atrophy is always associated with Dexamethasone (Dexa) treatment, however the mechanisms are not completely understood. This study investigated the effects of Dexa on myostatin and p70S6K protein expression and if previous exercise training (T) can attenuate these effects. Eighty rats were distributed into 4 groups: sedentary control (SC), sedentary treated with Dexa (SD; 0,5 mg/kg per day, i.p., 10 days), trained control (TC) and trained treated with Dexa (TD) and underwent a training period where they were either submitted to a running protocol (60% of physical capacity, 5 days/week for 8 weeks) or kept sedentary. After T period, animals underwent Dexa treatment concomitant with training. Western Blot was performed to identify myostatin and p70S6k protein expression in the tibialis anterior (TA) and soleus (SOL) muscle. Ten days of Dexa treatment increased fasting glucose (SD=+62%), however previous T attenuated this increase (TD=+20%, p<0.05). Dexa determined significant decrease in body weight in TD (-22%) and SD (-25%), followed by TA weight reduction in SD (-23%) and TD (-20%). Previous training could not avoid these decreases. Myostatin protein expression was not altered by dexa treatment or training in TA muscle but in SOL muscle it was significantly modified after T, regardless of treatment (TC=+%23 and TD=+25) compared with their respective controls. The protein p70S6K was not modified neither by dexa nor training in any of the analyzed muscle or condition. The results of this study allowed us to conclude that previous training attenuates the hyperglycemia induced by Dexa, however it did not prevent the body or muscle weight reductions. Even in the presence of muscle atrophy, the expression of myostatin and p70S6K do not justify the mechanisms of muscle loss induced by Dexa, which suggests that other catabolic or anabolic proteins could be involved in the process of muscle atrophy after 10 days of treatment with Dexa

Avaliação do perfil antropométrico e de força de jovens jogadores de polo-aquático

The present study aimed to analyze the performance of 'boost' among water polo athletes. Twelve athletes (13,9  1,08 years, 169,0  6,0 cm and 59,5  13,5 kg of bodyweight) were under went to anthropometric assessment, body composition and maximum concentric strength of lower limbs. The jump test in the water was done by 'boost' and scaled to jump height. According to the results of strength (Leg Press 45: 157,08  43,30 kg Leg Extension: 72,08  12,86 kg), anthropometry (arm circumf.: 26,04  3,25 cm, thigh circumf.: 50,47  7,65 cm, sholder circumf.: 95,97  8,00 cm, elbow diam.: 4,74  0,51 cm, wrist diam.: 3,21  0,34 cm, knee diam.: 7,89  0,58 cm, biacromial diam.: 37,49  3,81 cm and bi-iliac diam.: 25,43  4,34 cm) and body composition (BMI: 20,74  4,00 kg / m² and% fat: 8,45  4,28), only stature showed a linear correlation (r= 0,595) to the values of vertical jump (height: 47,80  4,35 cm) performance. This anthropometric variable was one able to influence the height of performance by modifying it self, perhaps by maturity. However maturity may be an implicity feature of performance, since force is a concerned effect of the growth. Otherwise technique could play a mains role to the performance of the 'boost'. It can be conclude that possibly is advantageous for water polo athlete be higher and more mature for the realization of 'boost', and the training directed for the technique, but is necessary muscle fitness for the holder

Movimento dos olhos e controle postural

The control of stances such as the upright stance seems not to have a purpose in itself; this control could facilitate the execution of other simultaneous tasks, the so-called suprapostural tasks. The goal of this study was to determine the effects of saccadic eye movements on the control of posture. Twelve adult participants had their body oscillations analyzed while standing upright, for 70 s, in the postural conditions of feet apart and feet together, performing fixation in the central target or horizontal saccadic movements, in the conditions slow (0,5 Hz) and fast (1,1 Hz). The results showed that saccadic movements, independently of their frequency, strongly reduced trunk and head oscillations in the anterior-posterior (AP) axis. In this axis, there was an effect of feet position only in head oscillation. In the medio-lateral (ML) axis, the results showed a strong effect of feet position with body oscillation decreased in the condition of feet apart. The effect of the visual task in the ML axis occurred only for trunk oscillation, not reaching significance level in the pairewise comparisons. In the AP axis, the data corroborate a facilitatory explanation of the control of posture: the reduction in body oscillation limited the variations of the stimulus image projected on the retina, facilitating the execution of saccadic movements as compared to fixation. In the ML axis, the effect of reducing the basis of support was more evident than the effect of saccadic movements, suggesting that the available resources were used primarily for the postural task in detriment of the visual task. Additionally, aspects like attentional focus and sensory information pick up are discussed as mechanisms involved in this task

Controle visual da freada em ciclistas: efeitos da incerteza em situação de curva

The purpose of the present study was to analyze the visual control of braking a bicycle when the cyclist is surprised by an obstacle in his way. According to Lee (1976), visually controlled braking based on time to collision information utilizes the optic variables tau and its first derivative in time, tau-dot, to initiating the braking action and regulating its intensity. Seven young adults performed a bicycle braking task in curvilinear trajectory under distinct velocity (high, medium, and low) and uncertainty (certainty and uncertainty) conditions. Results showed that, independently of velocity and uncertainty levels, participants utilized tau and tau-dot to initiating and regulating the braking action, avoiding collision with the obstacle. Cognitive, attentional, and other psychological factors resulting from both increased velocity and uncertainty were not capable of altering the use of time to collision information, corroborating the tested hypothesis

Comparação entre a recuperação da economia de corrida e força muscular medida por diferentes métodos após o dano muscular

The goal of the present study was to investigate possible differences towards strenght recovery measured by different methods and running economy (RE), after one session of downhill running (DR), in order to induce muscle damage (MD). Our hypothesis is that the strenght recovery measured by jumps is more alike RE markers, due to the measures' specificity. Ten male subjects, ages 18 to 30, apparently healthy, without any experience with strenght training at least 6 months before the tests took place in this study. After going to the lab twice (to familiarize themselves with the protocols, and determine their maximum oxygen consumption/running speeds), they went to the lab five times in the following week, in order to collect all the variables before, immediately after, and 24, 48, 72 and 96 hours in. The variables obtained were: stride frequency (SF), stride lenght (SL), oxygen volume (VO2), carbon dioxide volume (VCO2), ventilation (VE), lactate blood concentration ([Lac]), isometric peak torque (IPT), subjective pain perception (SPP), effort perception (EP), medial-portion circumference (CIR), knee movement extent (EXT), torque development rate (TDR), counter movement jump and squat jump height (HCM and HS), strenght development rate of both jumps (SDCM and SDS), and maximum soil strenght reaction (SRCM and SRS). Changes over time in all variables were verified by one way variancy analysis. Differences between the strenght measures were verified by two way variancy analysis. When significant effects were verified, Tuckey's post-hoc were applied. The significancy level taken on this study was p < 0,05. Of all indirect muscle damage markers, IPT, SPP and TDR were the only ones in which ocurred significant changes. We couldn't find the moments where this happened for TDR with the post-hoc used. On RE markers, VO2, [Lac] and VE suffered significant effects over time. About the jumps variables, only SDCM and HCM presented significant...

Influência do método de avaliação no diagnóstico da dor muscular de início tardio após corrida em declive

Several methods are used towards delayed onset muscle soreness appraisal. This study's goal was to investigate, amongst three commonly adopted methods, which one would be the most effective (the one that shows higher values) in the quantification of this phenomenon. 10 male subjects, of age 22.8 ± 3.1 years old, weight 80 ± 12.4 kg, height 174 ± 0.07 cm, without recent experience with strength training (lower limbs) and/or running took place in this study. All subjects carried out a muscle damage induction protocol, which consisted of 30 minute downhill running (-16% or -9.09º) at 80% speed compared to their maximum oxygen consumption. Muscle damage determinants such as isometric peak torque, knee joint range of motion and circumference of the medial portion of the thight were measured before, during, 24, 48, 72 and 96 hours after downhill running. The subjective pain perception was measured simultaneously with the other determinants through three different tests: sitting on and getting up of a chair; climbing and descending from a 45cm step; and self thigh palpation. After going through all subjective pain perception tests, the subjects filled out a visual analog scale with their perception of pain. Muscle damage changes over time were compared through variance analysis (ANOVA) one way for repeated measures. Subjective pain perception values obtained in all three different tests were compared through two way ANOVAs for repeated numbers. The significance level adopted in this study was z ≤ 0.05. The results showed that the step test was the on which better evaluated the delayed onset muscle soreness. No significant differences were found through the ADM and CIR recovery markers. Downhil running determined reduced of PTI (~22.4%). Significant links between pain were obtained for both subjective pain perception tests starting at 24 and 48 hours, where the highest registered average happened in the step test after 48h, with high...

O exercício físico é capaz de melhorar expressão de AMPK e GLUT4 em músculo esquelético de ratos alcoolistas e/ou tabagistas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Prática supervisionada de exercícios físicos durante o tratamento regular com estatinas de pacientes da atenção básica: caracterização, aderência e delimitação das respostas musculares

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Repercussões cardiovasculares em crianças com distúrbios respiratórios obstrutivos do sono: revisão de literatura

Objective: To review the cardiovascular consequences of obstructive sleep-disordered breathing in children Data source: Medline and Ovid data base. Study selection: pediatric articles (original articles and case report) that investigated the obstructive sleep-disordered breathing in children Data synthesis: The obstructive sleep-disordered breathing can lead to serious cardiovascular consequences such as pulmonary hypertension, pulmonary edema and anatomic and functional abnormalities Conclusions: The knowledge of cardiovascular repercussions allows in better management of pediatric patients and may result in positive consequences for treatment and follow up of these patients.

Variação da temperatura superficial dos músculos masseter e temporal anterior durante o apertamento dental voluntário com placas interoclusais maxilares de duas diferentes espessuras

Muscle activity has been studied indirectly through analyses of temperature variation in skin surface temperature on the masticatory muscles. These procedure may be an important tool for clinical evaluation and assessment of the evolution of temporomandibular disorders, as well as monitoring the adopted protocol. Thus, its utilization for identified pathological alterations on blood circulation and/or on metabolic activity in subcutaneous tissues, such as the masticatory muscles, is justified. The aim of this study was to investigate the effect of occlusal splints’ thickness on the variation of surface temperature of the anterior temporal and masseter muscles during rest and clenching. It were analyzed 20 symptomatic subjects (10 males and 10 females) selected through the Research Diagnostic Criteria (RDC) questionnaire. Temperature expressed in degrees Celsius (ºC) was measured on the surface of both muscles measured with the aid of a digital thermometer with an infrared radiation reading system. The surface temperature of the anterior temporal and masseter muscles presented significant reduction during teeth clenching in relation to the mandibular rest position. However, there were no significant differences between conditions with and without occlusal splint or between the different thicknesses of splints analyzed. Masticatory muscles showed a considerable similar behavior for both sides, and no significant distinctions were observed between male and female patients.