Pax3 expression enhances PDGF-B-induced brainstem gliomagenesis and characterizes a subset of brainstem glioma.

High-grade Brainstem Glioma (BSG), also known as Diffuse Intrinsic Pontine Glioma (DIPG), is an incurable pediatric brain cancer. Increasing evidence supports the existence of regional differences in gliomagenesis such that BSG is considered a distinct disease from glioma of the cerebral cortex (CG). In an effort to elucidate unique characteristics of BSG, we conducted expression analysis of mouse PDGF-B-driven BSG and CG initiated in Nestin progenitor cells and identified a short list of expression changes specific to the brainstem gliomagenesis process, including abnormal upregulation of paired box 3 (Pax3). In the neonatal mouse brain, Pax3 expression marks a subset of brainstem progenitor cells, while it is absent from the cerebral cortex, mirroring its regional expression in glioma. Ectopic expression of Pax3 in normal brainstem progenitors in vitro shows that Pax3 inhibits apoptosis. Pax3-induced inhibition of apoptosis is p53-dependent, however, and in the absence of p53, Pax3 promotes proliferation of brainstem progenitors. In vivo, Pax3 enhances PDGF-B-driven gliomagenesis by shortening tumor latency and increasing tumor penetrance and grade, in a region-specific manner, while loss of Pax3 function extends survival of PDGF-B-driven;p53-deficient BSG-bearing mice by 33%. Importantly, Pax3 is regionally expressed in human glioma as well, with high PAX3 mRNA characterizing 40% of human BSG, revealing a subset of tumors that significantly associates with PDGFRA alterations, amplifications of cell cycle regulatory genes, and is exclusive of ACVR1 mutations. Collectively, these data suggest that regional Pax3 expression not only marks a novel subset of BSG but also contributes to PDGF-B-induced brainstem gliomagenesis.

Decision-Making in the Primate Brain

Making decisions is fundamental to everything we do, yet it can be impaired in various disorders and conditions. While research into the neural basis of decision-making has flourished in recent years, many questions remain about how decisions are instantiated in the brain. Here we explored how primates make abstract decisions and decisions in social contexts, as well as one way to non-invasively modulate the brain circuits underlying decision-making. We used rhesus macaques as our model organism. First we probed numerical decision-making, a form of abstract decision-making. We demonstrated that monkeys are able to compare discrete ratios, choosing an array with a greater ratio of positive to negative stimuli, even when this array does not have a greater absolute number of positive stimuli. Monkeys’ performance in this task adhered to Weber’s law, indicating that monkeys—like humans—treat proportions as analog magnitudes. Next we showed that monkeys’ ordinal decisions are influenced by spatial associations; when trained to select the fourth stimulus from the bottom in a vertical array, they subsequently selected the fourth stimulus from the left—and not from the right—in a horizontal array. In other words, they begin enumerating from one side of space and not the other, mirroring the human tendency to associate numbers with space. These and other studies confirmed that monkeys’ numerical decision-making follows similar patterns to that of humans, making them a good model for investigations of the neurobiological basis of numerical decision-making.

We sought to develop a system for exploring the neuronal basis of the cognitive and behavioral effects observed following transcranial magnetic stimulation, a relatively new, non-invasive method of brain stimulation that may be used to treat clinical disorders. We completed a set of pilot studies applying offline low-frequency repetitive transcranial magnetic stimulation to the macaque posterior parietal cortex, which has been implicated in numerical processing, while subjects performed a numerical comparison and control color comparison task, and while electrophysiological activity was recorded from the stimulated region of cortex. We found tentative evidence in one paradigm that stimulation did selectively impair performance in the number task, causally implicating the posterior parietal cortex in numerical decisions. In another paradigm, however, we manipulated the subject’s reaching behavior but not her number or color comparison performance. We also found that stimulation produced variable changes in neuronal firing and local field potentials. Together these findings lay the groundwork for detailed investigations into how different parameters of transcranial magnetic stimulation can interact with cortical architecture to produce various cognitive and behavioral changes.

Finally, we explored how monkeys decide how to behave in competitive social interactions. In a zero-sum computer game in which two monkeys played as a shooter or a goalie during a hockey-like “penalty shot” scenario, we found that shooters developed complex movement trajectories so as to conceal their intentions from the goalies. Additionally, we found that neurons in the dorsolateral and dorsomedial prefrontal cortex played a role in generating this “deceptive” behavior. We conclude that these regions of prefrontal cortex form part of a circuit that guides decisions to make an individual less predictable to an opponent.

‘Learning to be Zen’: Women travellers and the imperative to happy

This paper follows the emotional management of lone, independent women travellers as they move through tourist spaces, based on my doctoral research Embodiment and Emotion in the experiences of independent women tourists (2012). Specifically, this paper will focus on ‘gendering happiness’ by arguing that women travellers are significantly compelled to feel and display characteristics of happiness, humour and ‘learning to be Zen’ in order to be successful travellers. The imperative to become, and remain, happy and humorous in the face of embodied, emotional and gendered constraints is a key feature of women’s reflections of their travelling experiences, mirroring the recent emergence of literature into happiness and positive thinking within feminist theory (Ehrenreich 2010, Ahmed 2010). Negotiating ‘bad’ emotions provides a powerful insight into the perceptions of women travellers; to remain happy can mask problematic power relations and other forms of resistance. This is not to say that emotional negotiation is not partly a form of effective resistance, rather, I wish to make room for the freedom to be unhappy and angry in travelling space without feeling failure for not achieving a successful travelling identity.

Effects of a harmful algal bloom on the community ecology, movements and spatial distributions of fishes in a microtidal estuary

Harmful algal blooms can adversely affect fish communities, though their impacts are highly context-dependent and typically differ between fish species. Various approaches, comprising univariate and multivariate analyses and multimetric Fish Community Indices (FCI), were employed to characterise the perceived impacts of a Karlodinium veneficum bloom on the fish communities and ecological condition of the Swan Canning Estuary, Western Australia. The combined evidence suggests that a large proportion of the more mobile fish species in the offshore waters of the bloom-affected area relocated to other regions during the bloom. This was indicated by marked declines in mean species richness, catch rates and FCI scores in the bloom region but concomitant increases in these characteristics in more distal regions, and by pronounced and atypical shifts in the pattern of inter-regional similarities in fish community composition during the bloom. The lack of any significant changes among the nearshore fish communities revealed that bloom impacts were less severe there than in deeper, offshore waters. Nearshore habitats, which generally are in better ecological condition than adjacent offshore waters in this system, may provide refuges for fish during algal blooms and other perturbations, mirroring similar observations of fish avoidance responses to such stressors in estuaries worldwide.

Effects of a harmful algal bloom on the community ecology, movements and spatial distributions of fishes in a microtidal estuary

Harmful algal blooms can adversely affect fish communities, though their impacts are highly context-dependent and typically differ between fish species. Various approaches, comprising univariate and multivariate analyses and multimetric Fish Community Indices (FCI), were employed to characterise the perceived impacts of a Karlodinium veneficum bloom on the fish communities and ecological condition of the Swan Canning Estuary, Western Australia. The combined evidence suggests that a large proportion of the more mobile fish species in the offshore waters of the bloom-affected area relocated to other regions during the bloom. This was indicated by marked declines in mean species richness, catch rates and FCI scores in the bloom region but concomitant increases in these characteristics in more distal regions, and by pronounced and atypical shifts in the pattern of inter-regional similarities in fish community composition during the bloom. The lack of any significant changes among the nearshore fish communities revealed that bloom impacts were less severe there than in deeper, offshore waters. Nearshore habitats, which generally are in better ecological condition than adjacent offshore waters in this system, may provide refuges for fish during algal blooms and other perturbations, mirroring similar observations of fish avoidance responses to such stressors in estuaries worldwide.

La caractérisation du personnage Jésus dans la narration des Actes des Apôtres

Si la théologie lucanienne de la résurrection a largement été étudiée dans le livre des Actes des Apôtres, Jésus n’a jamais été considéré comme personnage principal, à l’inverse des apôtres, de l’Esprit saint ou même de Dieu. Pourtant, le premier verset des Actes laisse entendre que Jésus va continuer de faire et d’enseigner ce qu’il avait commencé dans l’évangile, même si, peu de temps après l’ouverture du récit, il quitte la scène. Pour chercher à comprendre ce paradoxe, une analyse approfondie de la mise en récit du personnage Jésus dans les Actes était nécessaire. Le premier chapitre de cette thèse introduit le sujet, l’état de la question et deux approches méthodologiques empruntées aux experts de la caractérisation narrative. Au chapitre deux, après avoir introduit la difficulté d’identification du personnage qui se trouve derrière le titre Seigneur des Actes, le cadre épistémologique d’Elizabeth Struthers Malbon permet d’observer le comment en classant toutes les péricopes qui participent à la rhétorique de la caractérisation christologique en cinq catégories : (1) la christologie représentée qui montre ce que Jésus fait, (2) la christologie détournée qui permet d’entendre ce que Jésus dit en réponse aux autres personnages, (3) la christologie projetée qui laisse entendre ce que les autres personnages ou le narrateur disent à Jésus et à son sujet, (4) la christologie réfléchie qui montre ce que les autres personnages font en reflétant ce que Jésus a dit et (5) la christologie reflétée qui montre ce que les autres personnages font en reflétant ce que Jésus a fait. Ensuite, avec le chapitre trois, l’approche de John Darr donne à comprendre le pourquoi de la caractérisation par l’observation de quatre activités cognitives du lecteur : (1) l’anticipation et la rétrospection, (2) la recherche de cohérence, (3) l’identification et/ou l’implication et (4) la défamiliarisation. Enfin, au chapitre quatre, les résultats des deux méthodes sont comparés pour proposer une solution au cas de l’ouverture paradoxale des Actes. D’abord les différentes observations de la thèse montrent que le personnage Jésus n’est pas si absent du récit; puis la rhétorique narrative de la caractérisation conduit le lecteur à comprendre que c’est essentiellement au travers des personnages du récit que Jésus est présent en actes et en paroles.

Influence of frequent nightmares on REM sleep-dependent emotional memory processing

La littérature suggère que le sommeil paradoxal joue un rôle dans l'intégration associative de la mémoire émotionnelle. De plus, les rêves en sommeil paradoxal, en particulier leur nature bizarre et émotionnelle, semblent refléter cette fonction associative et émotionnelle du sommeil paradoxal. La conséquence des cauchemars fréquents sur ce processus est inconnue, bien que le réveil provoqué par un cauchemar semble interférer avec les fonctions du sommeil paradoxal. Le premier objectif de cette thèse était de reproduire conceptuellement des recherches antérieures démontrant que le sommeil paradoxal permet un accès hyper-associatif à la mémoire. L'utilisation d'une sieste diurne nous a permis d'évaluer les effets du sommeil paradoxal, comparativement au sommeil lent et à l’éveil, sur la performance des participants à une tâche sémantique mesurant « associational breadth » (AB). Les résultats ont montré que seuls les sujets réveillés en sommeil paradoxal ont répondu avec des associations atypiques, ce qui suggère que le sommeil paradoxal est spécifique dans sa capacité à intégrer les traces de la mémoire émotionnelle (article 1). En outre, les rapports de rêve en sommeil paradoxal étaient plus bizarres que ceux en sommeil lent, et plus intenses émotionnellement ; ces attributs semblent refléter la nature associative et émotionnelle du sommeil paradoxal (article 2). Le deuxième objectif de la thèse était de préciser si et comment le traitement de la mémoire émotionnelle en sommeil paradoxal est altéré dans le Trouble de cauchemars fréquents (NM). En utilisant le même protocole, nos résultats ont montré que les participants NM avaient des résultats plus élevés avant une sieste, ce qui correspond aux observations antérieures voulant que les personnes souffrant de cauchemars soient plus créatives. Après le sommeil paradoxal, les deux groupes, NM et CTL, ont montré des changements similaires dans leur accès associatif, avec des résultats AB-négatif plus bas et AB-positif plus grands. Une semaine plus tard, seul les participants NM a maintenu ce changement dans leur réseau sémantique (article 3). Ces résultats suggèrent qu’au fil du temps, les cauchemars peuvent interférer avec l'intégration de la mémoire émotionnelle pendant le sommeil paradoxal. En ce qui concerne l'imagerie, les participants NM avaient plus de bizarrerie et plus d’émotion positive, mais pas négative, dans leurs rêveries (article 4). Ces attributs intensifiés suggèrent à nouveau que les participants NM sont plus imaginatifs et créatifs à l’éveil. Dans l'ensemble, les résultats confirment le rôle du sommeil paradoxal dans l'intégration associative de la mémoire émotionnelle. Cependant, nos résultats concernant le Trouble de cauchemars ne sont pas entièrement en accord avec les théories suggérant que les cauchemars sont dysfonctionnels. Le groupe NM a montré plus d’associativité émotionnelle, de même que plus d'imagerie positive et bizarre à l’éveil. Nous proposons donc une nouvelle théorie de sensibilité environnementale associée au Trouble de cauchemar, suggérant qu'une sensibilité accrue à une gamme de contextes environnementaux sous-tendrait les symptômes uniques et la richesse imaginative observés chez les personnes souffrant de cauchemars fréquents. Bien que davantage de recherches doivent être faites, il est possible que ces personnes puissent bénéficier e milieux favorables, et qu’elles puissent avoir un avantage adaptatif à l'égard de l'expression créative, ce qui est particulièrement pertinent lorsque l'on considère leur pronostic et les différents types de traitements.

Pytanie o pamięć. Relacje autobiograficzne więźniów obozów koncentracyjnych

This book consists of two main parts. The first part offers a basic methodological introduction, presenting a concise but multifaceted overview of current problems of collective memory. The second part contains a set of interviews with former prisoners of concentration camps carried out by the authors. The research was conducted by Paweł Greń and Łukasz Posłuszny and focuses on issues of collective and cultural memory illustrated by individual life experiences of concentration camps prisoners. The field of oral history serves as the framework of analysis and narrative inquiry as its research tool. Interviews and additional research materials were collected by the authors and are not available in previous publications, making this work a precious supplement to the current scholarly body of knowledge and achievements in the discipline of memory studies. According to the authors, current historical and literary publications provide an incomplete picture of the WWII and its aftermaths for survivors, because descriptions of the war and imprisonment in the camp play still a dominate role in narratives. The importance of these issues in autobiographies is unquestionable and highly needed to create a common identity among generation of prisoners, though authors often wanted to perceive the fate of individuals in a broader perspective – including the periods before and after the war. Hence, interviews stressed personal experiences and their understanding over time by former prisoners. The interviews covered many topics on life before, during and after the camp – among them daily and neutral routines, but also difficult matters. The latter were connected on the one hand with traumatic events or harsh memories and emotions, and on the other hand with less extensively highlighted threads of prisoners’ lives - such as issues of the body and sexuality – and their dependence on particular representation or narrative. The authors are convinced that the book serves not only as a record of past remembered by eyewitnesses, but it also depicts their accounts in wider contexts and discourses, which expose specific dimensions of told and written stories. In the book Questions for Memory one examine the approach proposed by young scholars. Interviews were conducted from 2009-2011, seventy years after the end of the second world war, and this initiative was the result of questions and doubts of the authors from the existing literature. They also wanted to use the unique opportunity to meet with eyewitnesses and record their stories, because when they pass away we will irretrievably lose the possibility to listen to them and to pose sensitive questions. The majority of the interviewees were prisoners of KL Auschwitz-Birkenau, and their experiences differed greatly from each other based on social background and specific experience in the camps as well as their post-camp and postwar life. Aside from persons whose stories are already well known and open, readers will hear the stories of those who spoke only reluctantly and very rarely, or who had remained silent until the present author’s research. Qualitative differences between interviews occurred on the level of established relationship and atmosphere of trust, which varied according to circumstances and individual character and personality. For P. Greń and Ł. Posłuszny, each interviewed person is equally and highly valued due to the collected material and the personal experience of the meetings. Among the ten interviews placed in the book, seven of them are the stories told by women. Their testimonies exemplify realities of everyday prisoners’ existence and gravitate towards mirroring specifically feminine perspectives of imprisonment. For women, crucial problems stemmed from experiences of body that intertwine with suffering, feeling of shame and humiliation. Early discussions on holocaust literature and issues of representation that shaped the Polish narrative and collective memory imposed imperatives of silence on certain topics. A solution for reconciling heroic and inhuman deeds in stories with completely human physiology was impossible and improper for many years. There were also questions about life after, ways of dealing with a trauma or reflections on the present time. During conversations the authors attempted to come closer to something distant and incomprehensible for their generation and for people who did not experience the camps. Despite the fact that there have been seventy years of dealing with these events in literature, art, drama, film, memoirs and scientific works, the past still breeds more questions than answers. The book Questions for Memory serves as an example of this phenomenon.

Myeloid precursors, osteoclastogenesis, and Spondyloarthropathies

Spondyloarthropathies (or Spondyloarthritides; SpAs) are a group of heterogeneous but genetically related inflammatory disorders in which ankylosing spondylitis (AS) is considered the prototypic form. Among the genes associated with AS, HLA-B27 allele has the strongest association although the cause is still not clear. Rats transgenic for the human HLA-B27 gene (B27 rats) develop a systemic inflammation mirroring the human SpA symptoms and thus provide a useful model to study the contribution of this MHC class I molecule in the disease development. Of particular interest was the observation of absence of arthritis in B27 rats grown in germ-free conditions and a recent theory suggests that microbial dysbiosis and gut inflammation might play a key role in initiating the HLA-B27-associated diseases. Studies in our laboratory have previously demonstrated that HLA-B27 expression alters the development of the myeloid compartment within the bone marrow (BM) in B27 rat and causes loss of a specific dendritic cell (DC) population involved in self-tolerance mechanisms within the gut. The aim of this thesis was to further analyse the myeloid compartment in B27 rats with a particular focus on the osteoclast progenitors and the bone phenotype and to link this to the gut inflammation. In addition, translational studies analysed peripheral monocyte/pre-osteoclasts in AS patients and teased apart the role of cytokines in in vitro human osteoclast differentiation. To understand the dynamics of the myeloid/monocyte compartment within the B27-associated inflammation, monocytes within the bloodstream and BM of B27 rats were characterised via flow cytometry and their ability to differentiate into osteoclast was assessed in vitro. Moreover, an antibiotic regime was used to reduce the B27 ileitis and to evaluate whether this could affect the migration, the phenotype, and the osteoclastogenic potential of B27 monocytes. B27 animals display a systemic and central increase of “inflammatory” CD43low MOs, which are the main contributors to osteoclastogenesis in vitro. Antibiotic treatment reduced ileitis and also reverted the B27 monocyte phenotype. This was also associated with the reduction of the previous described TNFα-enhancement of osteoclast differentiation from B27 BM precursors. These evidences support the idea that in genetically susceptible individuals inflammation in the gut might influence the myeloid compartment within the BM; in other terms, pre-emptively educate precursor cells to acquire specific phenotype end functions after being recruited into the tissue. This might explain the enhanced differentiation of osteoclast from B27 BM progenitors and thus the HLA-B27-associated bone loss. The data shown in this thesis suggest a link between the immunity within the gut and BM haematopoiesis. This provides an attractive and novel research prospective that could help not only to increase the understanding of the HLA-B27-associated aetiopathogenesis but also to unravel the cellular crosstalk that allows the mucosal immunity to program central cell differentiation. Human translational studies on monocyte subsets, cytokines and cytokine network in AS osteoclastogenesis evidenced altered osteoclast differentiation in the presence of IL-22 although no differences in the phenotype and functions of circulating CD14+ monocytes were observed. In addition, studies on the role of TNFα and TNFRs showed a dual role of this inflammatory cytokine in the human OC differentiation. In particular, the activation of TNFR1 in monocytes in early osteoclastogenesis inhibits OC differentiation while TNFα-biasing for TNFR2 on osteoclast precursors mediates the osteoclastogenic effect. Whether similar mechanisms are involved in the TNFα-mediated joint destruction in human rheumatic diseases needs further investigations. This could contribute to the development of novel and more specific anti-TNFα agents for the treatment of bone erosion. In conclusion, taken together my studies support the idea of a crosstalk between the periphery and the central system during the inflammatory response and provide new insights to the mechanisms behind the enhancement of osteoclastogenesis in B27-associated disorders.

La caractérisation du personnage Jésus dans la narration des Actes des Apôtres

Si la théologie lucanienne de la résurrection a largement été étudiée dans le livre des Actes des Apôtres, Jésus n’a jamais été considéré comme personnage principal, à l’inverse des apôtres, de l’Esprit saint ou même de Dieu. Pourtant, le premier verset des Actes laisse entendre que Jésus va continuer de faire et d’enseigner ce qu’il avait commencé dans l’évangile, même si, peu de temps après l’ouverture du récit, il quitte la scène. Pour chercher à comprendre ce paradoxe, une analyse approfondie de la mise en récit du personnage Jésus dans les Actes était nécessaire. Le premier chapitre de cette thèse introduit le sujet, l’état de la question et deux approches méthodologiques empruntées aux experts de la caractérisation narrative. Au chapitre deux, après avoir introduit la difficulté d’identification du personnage qui se trouve derrière le titre Seigneur des Actes, le cadre épistémologique d’Elizabeth Struthers Malbon permet d’observer le comment en classant toutes les péricopes qui participent à la rhétorique de la caractérisation christologique en cinq catégories : (1) la christologie représentée qui montre ce que Jésus fait, (2) la christologie détournée qui permet d’entendre ce que Jésus dit en réponse aux autres personnages, (3) la christologie projetée qui laisse entendre ce que les autres personnages ou le narrateur disent à Jésus et à son sujet, (4) la christologie réfléchie qui montre ce que les autres personnages font en reflétant ce que Jésus a dit et (5) la christologie reflétée qui montre ce que les autres personnages font en reflétant ce que Jésus a fait. Ensuite, avec le chapitre trois, l’approche de John Darr donne à comprendre le pourquoi de la caractérisation par l’observation de quatre activités cognitives du lecteur : (1) l’anticipation et la rétrospection, (2) la recherche de cohérence, (3) l’identification et/ou l’implication et (4) la défamiliarisation. Enfin, au chapitre quatre, les résultats des deux méthodes sont comparés pour proposer une solution au cas de l’ouverture paradoxale des Actes. D’abord les différentes observations de la thèse montrent que le personnage Jésus n’est pas si absent du récit; puis la rhétorique narrative de la caractérisation conduit le lecteur à comprendre que c’est essentiellement au travers des personnages du récit que Jésus est présent en actes et en paroles.

Influence of frequent nightmares on REM sleep-dependent emotional memory processing

La littérature suggère que le sommeil paradoxal joue un rôle dans l'intégration associative de la mémoire émotionnelle. De plus, les rêves en sommeil paradoxal, en particulier leur nature bizarre et émotionnelle, semblent refléter cette fonction associative et émotionnelle du sommeil paradoxal. La conséquence des cauchemars fréquents sur ce processus est inconnue, bien que le réveil provoqué par un cauchemar semble interférer avec les fonctions du sommeil paradoxal. Le premier objectif de cette thèse était de reproduire conceptuellement des recherches antérieures démontrant que le sommeil paradoxal permet un accès hyper-associatif à la mémoire. L'utilisation d'une sieste diurne nous a permis d'évaluer les effets du sommeil paradoxal, comparativement au sommeil lent et à l’éveil, sur la performance des participants à une tâche sémantique mesurant « associational breadth » (AB). Les résultats ont montré que seuls les sujets réveillés en sommeil paradoxal ont répondu avec des associations atypiques, ce qui suggère que le sommeil paradoxal est spécifique dans sa capacité à intégrer les traces de la mémoire émotionnelle (article 1). En outre, les rapports de rêve en sommeil paradoxal étaient plus bizarres que ceux en sommeil lent, et plus intenses émotionnellement ; ces attributs semblent refléter la nature associative et émotionnelle du sommeil paradoxal (article 2). Le deuxième objectif de la thèse était de préciser si et comment le traitement de la mémoire émotionnelle en sommeil paradoxal est altéré dans le Trouble de cauchemars fréquents (NM). En utilisant le même protocole, nos résultats ont montré que les participants NM avaient des résultats plus élevés avant une sieste, ce qui correspond aux observations antérieures voulant que les personnes souffrant de cauchemars soient plus créatives. Après le sommeil paradoxal, les deux groupes, NM et CTL, ont montré des changements similaires dans leur accès associatif, avec des résultats AB-négatif plus bas et AB-positif plus grands. Une semaine plus tard, seul les participants NM a maintenu ce changement dans leur réseau sémantique (article 3). Ces résultats suggèrent qu’au fil du temps, les cauchemars peuvent interférer avec l'intégration de la mémoire émotionnelle pendant le sommeil paradoxal. En ce qui concerne l'imagerie, les participants NM avaient plus de bizarrerie et plus d’émotion positive, mais pas négative, dans leurs rêveries (article 4). Ces attributs intensifiés suggèrent à nouveau que les participants NM sont plus imaginatifs et créatifs à l’éveil. Dans l'ensemble, les résultats confirment le rôle du sommeil paradoxal dans l'intégration associative de la mémoire émotionnelle. Cependant, nos résultats concernant le Trouble de cauchemars ne sont pas entièrement en accord avec les théories suggérant que les cauchemars sont dysfonctionnels. Le groupe NM a montré plus d’associativité émotionnelle, de même que plus d'imagerie positive et bizarre à l’éveil. Nous proposons donc une nouvelle théorie de sensibilité environnementale associée au Trouble de cauchemar, suggérant qu'une sensibilité accrue à une gamme de contextes environnementaux sous-tendrait les symptômes uniques et la richesse imaginative observés chez les personnes souffrant de cauchemars fréquents. Bien que davantage de recherches doivent être faites, il est possible que ces personnes puissent bénéficier e milieux favorables, et qu’elles puissent avoir un avantage adaptatif à l'égard de l'expression créative, ce qui est particulièrement pertinent lorsque l'on considère leur pronostic et les différents types de traitements.

Indícios no insólito : Avalovara através do espelho

Tese (doutorado)—Universidade de Brasília, Instituto de Letras, Departamento de Teoria Literária e Literatura, Programa de Pós-Graduação em Literatura, 2015.

A relação diagnóstica em contexto de creche e jardim-de-infância

O presente estudo enquadra-se no domínio das Ciências da Educação e na área específica da Análise da Acão Educativa. Não se afastando das preocupações inscritas nos objetivos de um mestrado de autor "A Criança em Diferentes Contextos Educativos”, também se compagina com as nossas determinações pessoais e profissionais, empreendidas na convicção da investigação como um projeto de vida. Para o efeito, arquitetámos o nosso universo de estudo a partir da observação naturalista em sete salas de creche e de jardim-de-infância do distrito de Évora. Da população estudada fizeram parte diferentes acores educativos, entre eles, as crianças dos 3 meses aos 6 anos de idade, as educadoras e as auxiliares de Acão educativa. Pretendemos, a partir dos sinais emitidos pelas crianças, retirar indicadores de necessidades educativas, para compreender as atitudes e comportamentos dos agentes educativos (educadores e auxiliares) na sua prática pedagógica, inferindo das suas próprias necessidades formativas. Procurámos saber como eram feitos os diagnósticos de necessidades (educativas das crianças e formativas dos educadores) e como organizavam a sua relação com outros acores educativos, no sentido de um desenvolvimento holístico da criança. Dada a natureza e o objeto de estudo, optámos por uma metodologia qualitativa de carácter interpretativo, configurada num estudo de caso. Utilizámos a linha de Investigação-Acão/Formação, preconizada por Luís Barbosa, integrada na sua "Escola Sensível e Transformacionista", que radica os seus fundamentos na ideia de que o jardim-de-infância, tal como outra qualquer organização educativa, deve ser um "Observatório de Caracterização de necessidades educativas das crianças e formativas dos sues agentes educativos”, gerido na perspetiva da mudança. Neste enquadramento de ideias, ao qual aderimos por convicção, utilizámos, para caracterizar o real, a Observação como técnica privilegiada, pese embora as limitações que lhe são subjacentes. Minimizámos este facto, triangulando os dados com entrevistas semiestruturadas e notas de campo. Suportámo-nos da Técnica do Espelhamento, da Pedagogia de Ajuda, da Renomeação de Experiências e da Extensibilidade de Si, às quais, pela sua importância demos ênfase no enquadramento teórico. O nosso estudo permitiu-nos inferir que, a relação diagnostica nos processos de ensino/aprendizagem, naqueles contextos, parece estar correlacionada com o facto de que os agentes educativos ainda necessitam de adquirir um leque variado de competências, relacionadas com o Saber-Ser e o Saber-Estar, que lhes permitam atitudes relacionais pró-activas e maior sensibilidade para captar as emoções e as sensações das crianças. Este facto vem corroborar o facto de termos verificado que os agentes educativos não usam o diagnóstico de necessidades como âncora de organização das suas práticas educativas o que não contribui de forma sustentável para a promoção do bem-estar e do desenvolvimento integral da criança. ABSTRACT: The present study lies within the field of Educational Sciences in the specific area of analysis of education. Not moving away from the concerns listed in the goals of an author master degree "The Child in Different Educational Contexts”, it also fits our personal and professional decisions, undertaken in the belief of research as a project of life. We chose the universe of our study from naturalistic observation in seven rooms of Nurseries and Infant Schools in the district of Évora. The study population consisted of children from three months to six years old, educators and assistants of education. From signals emitted by children, we intended to take indicators of educational needs to understand the attitudes and behavior of the educators and their assistants in their pedagogical practice, inferring their own educational needs. We tried to know how the diagnoses of needs were made (educational needs of the children and training needs of the educators) and how they organized their relationship with other educational agents towards a holistic development of children. Considering the nature and purpose of the study we opted for a qualitative methodology of interpretative nature, in a case study. We used the action research training line, advocated by Luís Barbosa in his work "Escola Sensível e Transformacionista" (Sensitive and Transformational School), whose reason lies in the idea that Infant Schools, like other educational organizations, should be centers for the characterization of children’s educational needs and educational agents' formative needs, managed in terms of change. ln this framework of ideas, that we adopted by conviction, we used the observation as the main technique, despite its limitations. To minimize them we triangulated the data with semi structured interviews and field notes. We used the techniques of Mirroring, Pedagogy of Help, renaming of Experiences and Extensibility of Yourself, and, because of their importance, we focused on them in the theoretical framework. This study allowed us to infer that the diagnostic relationship in the teaching­learning processes, in those contexts, seem to be correlated with the fact that the educational agents still need to acquire a wide range of skills related to the Know How To Be, to provide them with proactive relational attitudes and a bigger sensibility to get the children's emotions and sensations. We also found that the educational agents don't use a diagnostic of needs as anchor of the organization of their educational practices, what does not contribute in a sustainable way for the promotion of the well being and integral development of the child.

Intervenções de enfermagem na melhoria das competências dos Adjuntos de ação directa sobre os cuidados de higiene e conforto à pessoa dependente

Relatório de estágio apresentado para a obtenção do grau de Mestre em Enfermagem Comunitaria

Intervenções de enfermagem na melhoria das competências dos Adjuntos de ação directa sobre os cuidados de higiene e conforto à pessoa dependente

Relatório de estágio apresentado para a obtenção do grau de Mestre em Enfermagem Comunitaria