Molecular variation of Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda : Pseudophyllidea) in different fish host species based on ITS rDNA sequences

The molecular variation in Bothriocephalus acheilognathi Yamaguti, 1934 from 11 species of freshwater fish collected in Australia, China, the Czech Republic, England and Hawaii was investigated by determining the nucleotide sequences of the internal transcribed spacer region. The length of the first and second internal transcribed spacer sequences of multiple individuals ranged from 553 to 571 bp and 553 to 615 bp, and the G + C content from 53.1 to 53.5%. The percentage sequence divergence varied between 0 and 0.9% in the ITS1 and 0 and 6.6% in the ITS2, respectively, indicating the occurrence of intraspecific variation. It is demonstrated that the fragment length variation resulted primarily from microsatellite polymorphisms present in the ITS region, especially in the ITS2 region. Phylogenetic analyses revealed that B. acheilognathi examined in this study consisted of three closely related genotypes with certain degrees of host-specificity, and the genotype representing isolates from Cyprinus carpio L. was the most common and diverse form within the species B. acheilognathi.

In Memoriam REv. Archibald Campbell Foss

Memorial address.

Christian missions ; their agents, and their results / by T.W.M. Marshall.

http://www.archive.org/details/a592254601marsuoft

Christian missions : their agents and their results (vol. 1) / by T.W.M. Marshall.

http://books.google.com/books?vid=ISBN0665456816&id=sipohllLjKQC&dq=protestant+missions&a_sbrr=1 View book via Google

Christian missions and a new world culture

http://www.archive.org/details/christianmission027881mbp

Christian missions : their agents and their results (vol. 2) / by T.W.M. Marshall.

http://books.google.com/books?vid=ISBN0665456816&id=sipohllLjKQC&dq=protestant+missions&a_sbrr=1 View book via Google

For the faith, life of Just de Brenteniáeres, martyred in Korea, March 8, 1866; adapted from the French of C. Appert, by Florence Gilmore.

http://www.archive.org/details/forthefaithlifeo00appeuoft

Last letters & further records of martyred missionaries of the China Inland Mission / edited by Marshall Broomhall.

http://www.archive.org/details/lastlettersfurth00unknuoft

HIV-1 envelope gp41 antibodies can originate from terminal ileum B cells that share cross-reactivity with commensal bacteria.

Monoclonal antibodies derived from blood plasma cells of acute HIV-1-infected individuals are predominantly targeted to the HIV Env gp41 and cross-reactive with commensal bacteria. To understand this phenomenon, we examined anti-HIV responses in ileum B cells using recombinant antibody technology and probed their relationship to commensal bacteria. The dominant ileum B cell response was to Env gp41. Remarkably, a majority (82%) of the ileum anti-gp41 antibodies cross-reacted with commensal bacteria, and of those, 43% showed non-HIV-1 antigen polyreactivity. Pyrosequencing revealed shared HIV-1 antibody clonal lineages between ileum and blood. Mutated immunoglobulin G antibodies cross-reactive with both Env gp41 and microbiota could also be isolated from the ileum of HIV-1 uninfected individuals. Thus, the gp41 commensal bacterial antigen cross-reactive antibodies originate in the intestine, and the gp41 Env response in HIV-1 infection can be derived from a preinfection memory B cell pool triggered by commensal bacteria that cross-react with Env.