Realidade ou ficção? A influência da autopercepção como parceiro romântico e da autoestima na escolha e preferência de parceiros românticos

In general, men and women look for characteristics that can indicate improvement of their reproductive success in their romantic partners. However, partner choice is not only based on what the individual want in a partner, but he/she also takes into consideration how they perceive themselves in a determined environment, in a way that self-perception can change according to the context where the individual is inserted in. Besides the environment, self-esteem can be a factor that modifies romantic partner preferences and the way that people choose these partners for being able to influence how people evaluate themselves. Most of the studies that originate today’s universal standards in the study of romantic partner were conducted with undergraduate students, which may limit the coverage of the conclusions for contemplating people of the same educational level and probably also socioeconomic status (SES). The present research, held in Brazil, where the social inequality rate is high, and part in Canada, country with low social inequality, had as goal verifying romantic partners preferences and choices and self-evaluation as romantic partners in different educational level and SES. Men, mainly of low SES, tend to prioritize social status when looking for a romantic partner while the universal pattern is the preference for physical attractiveness, and women of low SES seem to be aware of this preference when expressing that social status is important for their self-perception. In addition, the results corroborate the influence of the context, selfesteem, and SES in the self-perception as a romantic partner, though the latter two appear to modulate how the is the influence of the context on participants’ perception. Moreover, this research also indicated that the preferences appear to represent the choices for the most important characteristics for each sex, being the other characteristics probably modulated by the quantity of available participants in the environment.

Naming the pain in requirements engineering: Contemporary problems, causes, and effects in practice

Requirements Engineering (RE) has received much attention in research and practice due to its importance to software project success. Its inter-disciplinary nature, the dependency to the customer, and its inherent uncertainty still render the discipline diffcult to investigate. This results in a lack of empirical data. These are necessary, however, to demonstrate which practically relevant RE problems exist and to what extent they matter. Motivated by this situation, we initiated the Naming the Pain in Requirements Engineering (NaPiRE) initiative which constitutes a globally distributed, bi-yearly replicated family of surveys on the status quo and problems in practical RE.

In this article, we report on the analysis of data obtained from 228 companies in 10 countries. We apply Grounded Theory to the data obtained from NaPiRE and reveal which contemporary problems practitioners encounter. To this end, we analyse 21 problems derived from the literature with respect to their relevance and criticality in dependency to their context, and we complement this picture with a cause-effect analysis showing the causes and effects surrounding the most critical problems.

Our results give us a better understanding of which problems exist and how they manifest themselves in practical environments. Thus, we provide a rst step to ground contributions to RE on empirical observations which, by now, were dominated by conventional wisdom only.

Qualidade de Vida e Atitudes dos Idosos Face à Velhice

A problemática do envelhecimento tem assumido, nos últimos anos, uma crescente importância na consciência coletiva da população, tornando-se cada vez mais importante compreender a população idosa e a sua realidade. Posto isto, foi realizado um estudo quantitativo e correlacional, que teve como objectivo avaliar a qualidade de vida e atitudes face à velhice de idosos, bem como a relação entre estas e as variáveis sociodemográficas e familiares. Foram inquiridos 100 idosos, com mais de 65 anos e sem deficit cognitivo . Para a recolha de dados utilizou-se uma entrevista estruturada, constituída dados sóciodemográficos do idoso, WHOQOL-AGE (Caballero, Miret, Power, Chatterji, Tobiasz-Adamczyk, Koskinen, Leonardi, Olaya, Haro &Ayuso-Mateos, 2013) e o AAQ ( Laidlaw, Power, Schmidt and the WHOQOL-OLD Group, 2007). Dos resultados destacamos os seguintes: A amostra é constituída por 52% de idosos do sexo masculino tendo uma média de idades de 74,7 (DP=6,8). È no fator Perdas Psicossociais e no Desenvolvimento Psicológico que os idosos têm uma melhor atitude face ao envelhecimento. É no item “Tem dinheiro suficiente para satisfazer as suas necessidades?” que os idosos apresentam uma menor qualidade de vida. Não ter doença diagnosticada e ser do sexo masculino permitem ter melhores atitudes face ao envelhecimento. A Qualidade de Vida está relacionada com a idade, com o estado de saúde e com a intensidade de preocupação da família. Constatou-se que os idosos que não estão institucionalizados apresentam uma melhor qualidade de vida e uma melhor atitude face à velhice. Quem não precisa de ajudas técnicas para se movimentar apresenta uma melhor qualidade de vida. Diferenças nas atitudes face ao envelhecimento consoante a residência onde habita são significativas nas mudanças físicas e no desenvolvimento psicológico sendo que os idosos que não vivem em lares têm uma atitude mais positiva em ambos os fatores. / Over the past few years the issue of aging has played a growing importance in the population`s collective consciousness becoming increasingly important to understand the elderly population and this reality. Therefore a quantitative correlational study was performed to assess the quality of life of seniors and their attitudes towards old age, and the relationship between these and the socio-demographic and family factors. 100 seniors with more than 65 years and without cognitive deficit were surveyed. For data collection we used a structured interview consisting of sociodemographic data of the elderly, WHOQOL-AGE (Caballero Miret Power Chatterji Tobiasz-Adamczyk Koskinen Leonardi Olaya Ayuso-Mateos & Haro 2013) and AAQ (Laidlaw Power Schmidt and the WHOQOL-OLD Group 2007). We highlight: The sample is composed of 52% of males with a mean age of 74.7 (SD = 6.8). It is in the factor Psychosocial Losses and Psychological Development that elderly people have a better attitude towards aging. It is in the item "Do you have enough money to meet your needs?" that seniors show less quality of life. Not having illness and being male allows having better attitudes towards aging. Quality of Life is related to age, health condition and the intensity of family concerns. It was observed that the elderly who are not institutionalized have a better quality of life and a better attitude towards old age. Who does not need assistive devices to move around has a better quality of life. Differences in attitudes towards aging, according to residency, are significant in physical changes and psychological development, thus verifying that elderly who do not live in nursing homes have a more positive attitude in both factors.

Estudo Preliminar de Adaptação e Validação da Escala de Avaliação do Eu Resiliente: resiliência, autodano e ideação suicida em adolescentes

Introdução: Em Portugal, são escassos os instrumentos validados para a população adolescente, que avaliem o importante construto da resiliência. Assim, o principal objetivo deste estudo consistiu na adaptação e validação preliminar da Escala de Avaliação do EU Resiliente (EAER) para adolescentes portugueses. Como segundo objetivo pretendemos, ainda, explorar as associações, na mesma amostra, entre a resiliência, o autodano e a ideação suicida na adolescência. Método: A amostra foi constituída por 226 adolescentes (sexo masculino, n = 139, 61,5%), entre os 12 e os 18 anos, que preencheram um protocolo composto por um questionário sociodemográfico, pela Escala de Avaliação do EU Resiliente (EAER), pelo Questionário de Impulso, Autodano e Ideação Suicida na Adolescência (QIAIS-A) e pela Escala de autoconceito. Resultados: Os resultados obtidos mostraram que a EAER possui boa fidelidade/consistência interna (α = 0,857) e boa estabilidade temporal (r = 0,720). Uma análise de componentes principais mostrou que a EAER apresenta três fatores: fator suporte externo, fator forças pessoais internas e fator estratégias de coping. Encontraram-se correlações negativas entre a resiliência e o autodano e ideação suicida e correlações positivas entre a resiliência e o autoconceito, confirmando-se a validade divergente e convergente da EAER. Verificaram-se níveis elevados de resiliência nos adolescentes da nossa amostra (M = 58,69; DP = 6,67). Na amostra total, 61,5% (n = 139) apresentou ideação suicida e 26,5% (n = 60) apresentou comportamentos de autodano. Conclusão: No seu conjunto, a EAER possui boas características psicométricas, pelo que pode ser considerada uma escala válida e útil e que pode ser usada com segurança na avaliação da resiliência em adolescentes portugueses. Com este estudo alargámos o leque de instrumentos válidos para a medição da resiliência em adolescentes e contribuímos para o avanço da investigação na área da adolescência em Portugal. / Introduction: In Portugal, there are few validated instruments to the adolescent population, to assess the important construct of resilience. Thus, the main objective of this study was the preliminary adaptation and validation of the Escala de Avaliação do EU Resiliente (EAER) to Portuguese adolescents. As a second objective, there is an intention to also explore the associations, on the same sample, between resilience, self-harm and suicidal ideation in adolescence. Method: The sample consisted of 226 adolescents (male, n = 139, 61.5%), between 12 and 18 years, who filled in a protocol consisting of a sociodemographic questionnaire, by the Escala de Avaliação do EU Resiliente (EAER), by the Impulse, Self-harm and Suicide Ideation Questionnaire for Adolescents (ISSIQ-A) and by the Self-concept Scale. Results: The results showed that the EAER has good fidelity/internal consistency (α = 0.857) and good temporal stability (r = 0.720). A principal component analysis showed that EAER has three factors: external support factor, internal personal strengths factor and coping strategies factor. There were negative correlations between resilience and the self-harm and suicidal ideation and positive correlations between resilience and self-concept, confirming the divergent and convergent validity of EAER. There were high levels of resilience in the adolescents of the sample (M = 58.69, SD = 6.67). In the total sample, 61.5% (n = 139) had suicidal ideation and 26,5% (n = 60) had self-harm behaviors. Conclusion: As a whole, the EAER has good psychometric properties, therefore it can be considered a valid and useful range, and can be safely used in the evaluation of resilience in Portuguese adolescents. With this study we have extended the range of valid instruments for the measurement of resilience in adolescents and contributed to the advance of research in the adolescence area in Portugal.

Hazelnut Allergens: Molecular Characterisation, Detection and Clinical Relevance

In the last years, special attention has been devoted to food-induced allergies, from which hazelnut allergy is highlighted. Hazelnut is one of the most commonly consumed tree nuts, being largely used by the food industry in a wide variety of processed foods. It has been regarded as a food with potential health benefits, but also as a source of allergens capable of inducing mild to severe allergic reactions in sensitised individuals. Considering the great number of reports addressing hazelnut allergens, with an estimated increasing trend, this review intends to assemble all the relevant information available so far on the main issues: prevalence of tree nut allergy, clinical threshold levels, molecular characterisation of hazelnut allergens (Cor a 1, Cor a 2, Cor a 8, Cor a 9, Cor a 10, Cor a 11, Cor a 12, Cor a 14 and Cor a TLP) and their clinical relevance, and methodologies for hazelnut allergen detection in foods. A comprehensive overview on the current data about the molecular characterisation of hazelnut allergens is presented, relating biochemical classification and biological function with clinical importance. Recent advances on hazelnut allergen detection methodologies are summarised and compared, including all the novel protein- and DNA-based approaches.

Development of a simple and fast “DNA extraction kit” for sea food identification and marine species

Seafood products fraud, the misrepresentation of them, have been discovered all around the world in different forms as false labeling, species substitution, short-weighting or over glazing in order to hide the correct identity, origin or weight of the seafood products. Due to the value of seafood products such as canned tuna, swordfish or grouper, these species are the subject of the commercial fraud is mainly there placement of valuable species with other little or no value species. A similar situation occurs with the shelled shrimp or shellfish that are reduced into pieces for the commercialization. Food fraud by species substitution is an emerging risk given the increasingly global food supply chain and the potential food safety issues. Economic food fraud is committed when food is deliberately placed on the market, for financial gain deceiving consumers (Woolfe, M. & Primrose, S. 2004). As a result of the increased demand and the globalization of the seafood supply, more fish species are encountered in the market. In this scenary, it becomes essential to unequivocally identify the species. The traditional taxonomy, based primarily on identification keys of species, has shown a number of limitations in the use of the distinctive features in many animal taxa, amplified when fish, crustacean or shellfish are commercially transformed. Many fish species show a similar texture, thus the certification of fish products is particularly important when fishes have undergone procedures which affect the overall anatomical structure, such as heading, slicing or filleting (Marko et al., 2004). The absence of morphological traits, a main characteristic usually used to identify animal species, represents a challenge and molecular identification methods are required. Among them, DNA-based methods are more frequently employed for food authentication (Lockley & Bardsley, 2000). In addition to food authentication and traceability, studies of taxonomy, population and conservation genetics as well as analysis of dietary habits and prey selection, also rely on genetic analyses including the DNA barcoding technology (Arroyave & Stiassny, 2014; Galimberti et al., 2013; Mafra, Ferreira, & Oliveira, 2008; Nicolé et al., 2012; Rasmussen & Morrissey, 2008), consisting in PCR amplification and sequencing of a COI mitochondrial gene specific region. The system proposed by P. Hebert et al. (2003) locates inside the mitochondrial COI gene (cytochrome oxidase subunit I) the bioidentification system useful in taxonomic identification of species (Lo Brutto et al., 2007). The COI region, used for genetic identification - DNA barcode - is short enough to allow, with the current technology, to decode sequence (the pairs of nucleotide bases) in a single step. Despite, this region only represents a tiny fraction of the mitochondrial DNA content in each cell, the COI region has sufficient variability to distinguish the majority of species among them (Biondo et al. 2016). This technique has been already employed to address the demand of assessing the actual identity and/or provenance of marketed products, as well as to unmask mislabelling and fraudulent substitutions, difficult to detect especially in manufactured seafood (Barbuto et al., 2010; Galimberti et al., 2013; Filonzi, Chiesa, Vaghi, & Nonnis Marzano, 2010). Nowadays,the research concerns the use of genetic markers to identify not only the species and/or varieties of fish, but also to identify molecular characters able to trace the origin and to provide an effective control tool forproducers and consumers as a supply chain in agreementwith local regulations.

Aplicação das ciências forenses no ensino secundário: um conjunto de actividades para a disciplina de Física e Química A

A popularidade das séries televisivas que abordam as Ciências Forenses (CSI 1 Mentes Criminosas, Casos Arquivados...) permite construir actividades que envolvem os alunos numa exploração científica de modo a que dominem os conceitos e apreciem a natureza da Ciência. Estes recursos educativos aplicam uma abordagem Ciência­ Tecnologia-Sociedade -Pensamento Crítico (CTS-PC) e podem ser explorados segundo uma metodologia de Aprendizagem Baseada na Resolução de Problemas (ABRP). Neste trabalho são propostas actividades laboratoriais, a decorrer no contexto formal das aulas da disciplina de Física e Química A (FQ·A), utilizando sensores e a calculadora gráfica. Num contexto não-formal, e baseadas nas técnicas analíticas utilizadas em Ciências Forenses, foram desenvolvidas actividades para um Clube de Ciência ou Laboratórios Abertos, complementadas por um blogue (CSI-Mafra). Estes recursos foram posteriormente partilhados com outros professores de Física e Química, numa acção de formação, proposta pela autora, e cujo objectivo principal foi a diversificação das estratégias de ensino e aprendizagem. O entusiasmo dos alunos na realização das tarefas propostas e os resultados nos questionários de grau de satisfação e de opinião, aplicados a alunos e professores, sugere que as actividades desenvolvidas podem contribuir na motivação dos alunos para o estudo da Física e da Química e promover a literacia científica da comunidade escolar. ABSTRACT; The high popularity between teenagers of the television series that have Forensic Sciences and Criminal lnvestigations as central theme, such as CSI, Dexter, Criminal Minds and Cold Case, can be used to develop learning activities that involves the students into a scientific exploration which aim is leading the students to learn Chemistry and Physics with pleasure, motivation and curiosity. The educational resources developed in this thesis make use of the strategy Science-Technology-Society-Critical Thinking (STS-CP) and can be exploited according the teaching methodology Problem Based Learning (PBL). ln this work we propose a number of laboratory activities that can be used on the classes of the High School course Physic and Chemistry A (FQ-A) and a different set of activities to be used in a non-formal teaching environment, which are based on the analytical techniques used in Forensic Sciences. The non-formal activities were developed as part of the activities of a Science Club and complemented with an interactive blog. The teaching resources developed by us were also used in a professional training course to physics and chemistry teachers aimed to teach how to introduce new teaching­ learning strategies. The enthusiasm of the students shown during the activities and the extremely positive results of the questionnaires, applied to students and teachers after the activities, clearly indicates that the learning resources developed in this thesis contribute to the student's motivation to learn Physic and Chemistry and to promote the scientific Iiteracy of the scholar community.

Desenvolvimento de nanosistemas farmacêuticos para terapia gênica

Gene therapy is one of the major challenges of the post-genomic research and it is based on the transfer of genetic material into a cell, tissue or organ in order to cure or improve the patient s clinical status. In general, gene therapy consists in the insertion of functional genes aiming substitute, complement or inhibit defective genes. The achievement of a foreigner DNA expression into a population of cells requires its transfer to the target. Therefore, a key issue is to create systems, vectors, able to transfer and protect the DNA until it reaches the target. The disadvantages related to the use of viral vectors have encouraged efforts to develop emulsions as non-viral vectors. In fact, they are easy to produce, present suitable stability and enable transfection. The aim of this work was to evaluate two different non-viral vectors, cationic liposomes and nanoemulsions, and the possibility of their use in gene therapy. For the two systems, cationic lipids and helper lipids were used. Nanoemulsions were prepared using sonication method and were composed of Captex® 355; Tween® 80; Spam® 80; cationic lipid, Stearylamine (SA) or 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP) and water (Milli-Q®). These systems were characterized by average droplet size, Polidispersion Index (PI) and Zeta Potential. The stability of the systems; as well as the DNA compaction capacity; their cytotoxicity and the cytotoxicity of the isolated components; and their transfection capacity; were also evaluated. Liposomes were made by hydration film method and were composed of DOTAP; 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), containing or not Rhodaminephosphatidylethanolamine (PE- Rhodamine) and the conjugate Hyaluronic Acid DOPE (HA-DOPE). These systems were also characterized as nanoemulsions. Stability of the systems and the influence of time, size of plasmid and presence or absence of endotoxin in the formation of lipoplexes were also analyzed. Besides, the ophthalmic biodistribution of PE-Rhodamine containing liposomes was studied after intravitreal injection. The obtained results show that these systems are promising non-viral vector for further utilization in gene therapy and that this field seems to be very important in the clinical practice in this century. However, from the possibility to the practice, there is still a long way

ROBOT CEMAR: identificación de las capacidades emprendedoras de sus creadores

El XXI Congreso Latinoamericano sobre Espíritu Empresarial, se realizó en la Universidad Icesi los días 6, 7 y 8 de abril de 2011.

Variabilidade da concentração média de CO2 acima da floresta Amazônica durante a noite associada a distintos regimes turbulentos.

Estudou-se a Camada Limite Noturna ? CLN sobre área de floresta tropical primária a nordeste do Estado do Amazonas, no Observatório Amazônico da Torre Alta ? OATA ou Amazon Tall Tower Observatory ? ATTO. Foi aplicada a metodologia disponível na literatura que permitiu a caracterização da CLN em três regimes turbulentos: 1º) de turbulência fraca, com velocidade média do vento baixa; 2º) de turbulência forte, com velocidade do vento alta e, 3º) turbulência intermitente e condições de não estacionariedade. Existem variações sazonais nos valores de ?c? e ao passar de um regime para outro. Foram investigadas algumas das principais características estatísticas das concentrações de CO2 ? ?c? de cada um dos regimes turbulentos. ?c? aumenta levemente com a elevação do valor de uma escala característica de velocidade turbulenta, VTKE, até um valor limiar, VL, a partir do qual tem-se um aumento súbito nos valores de ?c?. Esses resultados têm importantes consequências para a parametrização de grandezas meteorológicas na CLN e modelagem do escoamento atmosférico na interface floresta-atmosfera.

Origin of and resulting floristic composition from seedbanks in agroforestry systems of Tomé-Açu, Eastern Amazon.

2016

Intervir num espaço pré-existente através da luz, cor e materialidade

Dissertação para obtenção do grau de Mestre em Arquitetura com Especialização em Arquitetura de Interiores, apresentada na Universidade de Lisboa - Faculdade de Arquitetura.

Identificação e caracterização fisiológica do soldado nas operações em áreas edificadas

No âmbito do Trabalho de Investigação Aplicada foi elaborada uma caracterização fisiológica do soldado nas operações em áreas edificadas, tendo como objetivo geral contribuir para o quadro teórico e prático na área da motricidade humana, relativamente às aptidões fisiológicas, designadamente: a Frequência Cardíaca, o Consumo de Oxigénio máximo (VO2max) e o Lactato Sanguíneo. Neste estudo foi executada uma pista de combate em áreas edificadas, com o nível de dificuldade crescente, inicialmente sem e com carga (colete balístico) de seguida com o inimigo armado com airsoft e para finalizar, a esquadra de assalto com armas de airsoft. A pista desenrolou-se na Aldeia de Camões em Mafra. Para medição dos parâmetros em estudo, utilizou os monitores cardíacos Garmin Forerunner 310XT, que fornecem os valores das Frequências Cardíacas e o Lactete Plus, que nos dão os valores dos níveis de Lactato Sanguíneo. Verificou-se que não existe uma doutrina de referência que evidencie o treino físico orientado para o combate em áreas edificadas nos dias de hoje. Da análise dos resultados obtidos verificámos que a intensidade do esforço, durante a execução das pistas de combate, foi predominantemente realizada no regime anaeróbio. Com os resultados alcançados durante a realização da pista de combate, identificámos algumas das tarefas mais críticas na sua execução. Apurámos através dos níveis de lactato que a função do comandante de esquadra é de uma intensidade de esforço físico superior a exigida na restante esquadra de assalto.

Controlled release products for managing insect pests.

2016

Synbiotic supplementation promotes improvement of chronic diarrhea of unknown etiology in patient with chronic kidney disease and provides better outcomes in dialysis

Background: Chronic kidney disease (CKD) patients often have gastrointestinal symptoms which may result in malnutrition and a negative impact on their quality of life. Modulation of the gut microbiota can be a strategy to promote host health and homeostasis. Case report: The authors present a case of chronic diarrhea in a hemodialysis (HD) patient with an unknown etiology. After about one year and several failed interventions, synbiotic therapy was performed. The diarrhea episodes ceased after three months of daily supplementation and both biochemical and nutritional parameters improved. Synbyotic therapy promoted clinical benefits in this patient. Discussion: Therefore, this simple therapy may be a promising alternative in CKD and it should be tested in larger studies.