Estudo comparativo da microflora fúngica de doentes portadores de prótese total acrílica mucosuportada e implantosuportada

Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz

Estudo comparativo da microflora bacteriana de doentes portadores de próteses totais acrílicas mucosuportadas e implantosuportadas

Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz

Oral bacterial microflora associated with total acrylic dentures: implant supported vs mucus supported

Poster presented at the 1st International Congress of CiiEM - From Basic Sciences to Clinical Research. 27-28 November 2015, Egas Moniz, Caparica, Portugal.

Oral fungal microflora associated with implant-supported VS. mucous-supported dentures

Poster presented at the 1st International Congress of CiiEM - From Basic Sciences to Clinical Research, 27-28 November 2015, Egas Moniz, Caparica, Portugal.

Changes in oral microflora in prosthetic patients on hygiene protocol

Poster presented at the 1st International Congress of CiiEM - From Basic Sciences to Clinical Research, 27-28 November 2015, Egas Moniz, Caparica, Portugal.

Miocene microflora and palaeoclimate reconstructions from three sites in Bulgaria

In this study we reconstruct quantitatively the Middle to Upper Miocene climate evolution in the southern Forecarpathian Basin (Central Paratethys area, Northwest Bulgaria) by applying the coexistence approach to 101 well-dated palynofloras isolated from three cores. The climatic evolution is compared with changes in vegetation and palaeogeography. The Middle Miocene was a period of a subtropical/warm-temperate humid climate with mean annual temperature (MAT) between 16 and 18°C and mean annual precipitation (MAP) between 1100 and 1300 mm. Thereby, during the entire Middle Miocene a trend of slightly decreasing temperatures is observed and only small climate fluctuations occur which are presumably related to palaeogeographic reorganisations. The vegetation shows a corresponding trend with a decrease in abundance of palaeotropic and thermophilous elements. The Upper Miocene is characterised by more diverse climatic conditions, probably depending on palaeogeographic and global climatic transformations. The beginning of this period is marked by a slight cooling and a significant drying of the climate, with MAT 13.3-17°C and MAP 652-759 mm. After that, fluctuations of all palaeoclimate parameters occur displaying cycles of humid/dryer and warmer/cooler conditions, which are again well reflected in the vegetation. Our study provides a first quantitative model of the Middle-Upper Miocene palaeoclimate evolution in Southeastern Europe and is characterised by a relatively high precision and resolution with respect to the climate data and stratigraphy.

Methods to evaluate pesticide damage to the biomass of the soil microflora

Respiratory methods to estimate the amount of C in the soil microbial biomass and the relative contributions of prokaryotes and eukaryotes in the biomass were used to evaluate the influence of pesticides on the soil microflora. Experiments were conducted with 5 and 50 micrograms per gram of three fungicides, captan, thiram and verdesan. At 5 micrograms per gram they caused significant decreases (40%) in the biomass; the organomercury fungicide verdesan also caused a shift from fungal to bacterial dominance. Within 8 days, biomass in captan- and thiram-amended soils had recovered to that of controls. Although the fungal to bacterial balance was restored in verdesan-amended soils, biomass recovery was not complete. At 50 micrograms per gram the fungicides caused long-term decreases in the biomass and altered the relative proportions of the bacterial and fungal populations. Verdesan had the greatest effect on soil microbial biomass and competition.

Anaerobic digestion process for biogas and biomolecules production: microflora identification and characterization

The anaerobic process was efficient in organic matter removal. During the process, an interesting compound as quercetin was produced inside of reactor. Phylogenetic analysis showed the presence of phylotypes affiliated with gamma-Proteobacteria, Choroflexi, and Bacteroidetes. Archaea were represented by phylotypes belonging to the genus Methanosarcina and Methanosaeta.