Non-neutral evolution in non-LEE-encoded type III effectors of attaching and effacing Escherichia coli

Attaching and effacing Escherichia coli (AEEC) employ type III secretion system (T3SS) to secrete effector proteins into host cells and regulate their function. Here we have investigated T3SS genes of AEEC for non-neutral evolution. Our analysis revealed non-neutral evolution in three genes (nleE1, nleB2 and nleD) which encode effector proteins. These genes are located outside the locus of enterocyte effacement (LEE). In general, non-LEE effector genes show greater deviation from neutral evolution than LEE effector genes. These results suggest that effector genes located outside LEE are under greater selection pressure than those present in LEE. (C) 2012 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Evaluacion del cultivo de sandia (Citrullus lanatus L) variedad Mickey Lee utilizando sustratos mejorados y determinacion de los coeficientes "kc" y ky", bajo riego. Finca Las Mercedes, Managua, 2009

El presente trabajo de investigación se estableció entre los meses de Marzo– Junio 2009, bajo sistema de riego por aspersión en la finca las Mercedes, ubicada en Managua km 11 carretera norte, 2 km entrada al Carnic en las coordenadas 12°10’14” a 12°08’05” de latitud Norte y 86°10’22” a 86°09’44” longitud Oeste, a una altura de 56 msnm. Con el objetivo de evaluar el cultivo de la sandía (Citrullus lanatus L) bajo tres tipos de fertilizantes orgánicos y un sintético, y obtención de los coeficientes del cultivo “Kc” y de rendimiento “Ky”. Se estableció un bloque completo al azar, con cuatro tratamientos y cuatro repeticiones. Los tratamientos orgánicos evaluados son: compost a razón de 25 595 kg ha-1 , bocashi 15 087 kg ha-1 y biofertilizante con aplicaciones de 20 000 lt ha-1 y un tratamiento sintético (testigo): fertilizante completo (NPK) formula 12-30-10, a razón de 350 kg ha-1 más urea 46% a razón de 219 kg ha-1 , todos los abonos orgánicos fueron elaborados como parte de la investigación haciendo uso de los distintos materiales que se tienen en la finca para su elaboración. Los resultados de campos se sometió a un análisis de varianza (ANDEVA) y separación de medias mediante el uso de la prueba de Diferencias Mínimas Significativas (LSD α=0.05). Utilizando el Software estadístico Infostat versión 2008. Con base a los datos obtenidos se puede afirmar, que no hubo diferencia significativa para las variables de crecimiento, tomadas en momentos diferentes, sin embargo para la variable de rendimiento y sus componentes se encontró diferencia significativas en número de frutos por planta (5.13), diámetro del fruto (15.90 cm), peso del fruto (5.96 lb) y rendimiento (70 020 kg ha-1 ) logrando obtener el mejor resultado cuando el cultivo se fertilizó con sintético, Con respecto a las variables de riego con un volumen de agua de 526.5 mm ha-1 en todo el ciclo del cultivo y tomando en cuenta las etapas fenológicas se le aplicó mayor volumen de agua en la etapa de floración y fructificación que es donde el cultivo requiere mayor cantidad de agua, logrando para todos los tratamientos un coeficiente de cultivo uniforme y curva de Kc siguió el mismo patrón de cualquier cultivo comercial, presentando una fase inicial, de desarrollo, de madurez y senescencia; En el caso de la reducción del coeficiente de rendimiento de la sandía en los tratamientos orgánicos no se debe a un estrés hídrico o limitación de agua durante los periodos de floración y fructificación que son los períodos más sensibles a la sequía, mas bien corresponde al factor de fertilidad ya que el abono orgánico no va dirigido a liberar grandes cantidades de nutrientes para el cultivo de una sola vez.

En las raíces ideológicas de Montoneros : John William Cooke lee a Gramsci en Cuba

Desde 1945 la izquierda argentina no pudo resolver el enigma planteado por el hecho de que la clase obrera era peronista. Varios intentos teóricos fracasaron antes de que John William Cooke hiciera el suyo a mediados de los años sesenta. Influenciado por Gramsci, consideró que la clase obrera es una categoría histórica, por lo que el peronismo era la experiencia histórica de la clase obrera argentina y, como tal, revolucionario. Neutralizó el papel político de Perón convirtiéndolo en el símbolo de la identidad peronista. Por esto, la teoría de Cooke fue clave para que guerrilleros de izquierda se declararan peronistas sin que les importaran las opiniones e intenciones políticas de Perón. Las limitaciones de esa teoría quedaron expuestas cuando el general regresó a la Argentina y fue electo presidente por tercera vez.

Saline water intrusion from deep artesian sources in McGregor Isles area of Lee County, Florida

Upward leakage of saline water from an artesian aquifer below 1,500 feet has caused an increase in chloride concentration in the lower Hawthorn aquifer from less than 1,000 mg/1 (milligrams per liter) to values ranging from about 1,300 to 15,000 mg/1. Similarly the higher temperatures of the intruding water has caused an increase in water temperatures in the aquifer from 82"F to values ranging from 83 to 93"F. The intruding water moves upward either through the open bore hole of deep wells or test holes, or along a fault or fracture system, which has been identified in the area. From these points of entry into the lower Hawthorn aquifer, the saline water spreads laterally toward the south and southeast, but is generally confined to components of the fault system. The saline water moves upward from the lower Hawthorn aquifer into the upper Hawthorn aquifer through the open bore hole of wells, which connect the aquifers. This movement has resulted in an increase in chloride from less than 200 mg/1 in the unaffected parts of the upper Hawthorn aquifer to values commonly ranging from about 300 to more than 3,000 mg/1 in parts of the aquifer affected by upward leakage. The upper Hawthorn aquifer is the principal source of ground-water supply for public water-supply systems in western Lee County. Similar effects have been noted in the water-table aquifer, where chloride increased from less than 100 to concentrations ranging from about 500 to more than 5,000 mg/1. This was caused by the downward infiltration of water discharged at land surface from wells tapping the lower Hawthorn aquifer. The spread of saline water throughout most of the McGregor Isles area is continuing as of 1971. (40 page document)

Interim report on surface water resources and quality of waters in Lee County, Florida

Report seeks to address following questions: 1. Where within Lee County are surface supplies of water located? 2. What are the variations in this supply? 3. What can be done to provide better answers to questions 1 and 2 than are available at the present time? (PDF contains 76 pages.)

¿Quién lee las estadísticas oficiales?

Duración (en horas): De 41 a 50 horas. Destinatario: Estudiante y Docente

International trade in live, ornamental "fish" in the US & Florida

The importance of international markets as a source of live, ornamental “fish” supply is growing due to more stringent wild-harvest regulations in Florida. In addition, foreign markets are increasing in importance as a source of demand for Florida purveyors of live, ornamental “fish”. Florida plays an important role in this growing international market. Trends in imports and exports of live, ornamental “fish” are described for two primary data sets: U.S. Customs and U.S. Fish and Wildlife Service. These trends are described primarily for the 1994-98 period for Florida and the United States. Florida imports and exports are described for the two major ports: Miami and Tampa. The most important trading countries are also described. This information will help Florida purveyors of live, ornamental “fish” better understand the international markets upon which they have become more dependent. (PDF has 18 pages)

Charles M. Breder, Jr.: Palmetto Key, 1942

Charles M. Breder and his wife Ethel spent part of the summer of 1942 at the Palmetto Key field station, known today as Cabbage Key, on the west coast of Florida south of Charlotte Harbor. The Palmetto Key field station began in 1938 and ended in 1942 because of World War II. His Palmetto Key diary ran for 95 pages of notes, tables, diagrams, drawings, lists, and business records and this report presents a variety of fascinating entries. Diaries from other years all bear Breder's style of discipline, curiosity, humor, and speculations on nature. The diary was transcribed as part of the Coastal Estuarine Data/Document Rescue and Archeology effort for South Florida. (PDF contaons 24 pages)

Cloud computing for research - final report

Curtis+Cartwright Consulting Ltd, working with the University of Surrey and Professor Charles Oppenheim, has been commissioned by Jisc to investigate Cloud Computing for Research. This document is the final report, and is accompanied by a briefing paper which provides advice targeted at researchers.

Charles M. Breder, Jr.: Hypothetical considerations, 1931-1937

Charles M. Breder Jr. “hypothesis” diary is a deviation from the field diaries that form part of the Breder collection housed at the Arthur Vining Davis Library, Mote Marine Laboratory. There are no notes or observations from specific scientific expeditions in the document. Instead, the contents provide an insight into the early meticulous scientific thoughts of this biologist, and how he examines and develops these ideas. It is apparent that among Dr. Breder’s passions was his continual search for knowledge about questions that still besieged many scientists. Topics discussed include symmetry, origin of the atmosphere, origin of life, mechanical analogies of organisms, aquaria as an organism, astrobiology, entropy, evolution of species, and other topics. The diary was transcribed as part of the Coastal Estuarine Data/Document Rescue and Archeology effort for South Florida. (PDF contains 33 pages)

Charles M. Breder, Jr.: Dry Tortugas, 1929

During the summer of 1929, Dr. Charles M. Breder, Jr., employed at that time by the New York Aquarium and American Museum of Natural History, visited the Carnegie Laboratory in the Dry Tortugas to study the development and habits of flying fishes and their allies. The diary of the trip was donated to the Mote Marine Laboratory Library by his family. Dr. Breder's meticulous handwritten account gives us the opportunity to see the simple yet great details of his observations and field experiments. His notes reveal the findings and thoughts of one of the world's greatest ichthyologists. The diary was transcribed as part of the Coastal Estuarine Data/Document Rescue and Archeology effort for South Florida. (PDF contains 75 pages)

Charles M. Breder, Jr.: Bahamas and Florida

Dr. Charles M. Breder, a well known ichthyologist, kept meticulous field diaries throughout his career. This publication is a transcription of field notes recorded during the Bacon Andros Expeditions, and trips to Florida, Ohio and Illinois during the 1930s. Breder's work in Andros included exploration of a "blue hole", inland ecosystems, and collection of marine and terrestrial specimens. Anecdotes include descriptions of camping on the beach, the "filly-mingoes" (flamingos) of Andros Island, the Marine Studios of Jacksonville, FL, a trip to Havana, and the birth of seahorses. This publication is part of a series of transcriptions of Dr. Breder's diaries. (PDF contains 55 pages)

Charles M. Breder, Jr.: Atlantis Expedition, 1934

Dr. Charles M. Breder participated on the 1934 expedition of the Atlantis from Woods Hole, Massachusetts to Panama and back and kept a field diary of daily activities. The Atlantis expedition of 1934, led by Prof. A. E. Parr, was a milestone in the history of scientific discovery in the Sargasso Sea and the West Indies. Although naturalists had visited the Sargasso Sea for many years, the Atlantis voyage was the first attempt to investigate in detailed quantitative manner biological problems about this varying, intermittent ‘false’ bottom of living, floating plants and associated fauna. In addition to Dr. Breder, the party also consisted of Dr. Alexander Forbes, Harvard University and Trustee of the Woods Hole Oceanographic Institution (WHOI); T. S. Greenwood, WHOI hydrographer; M. D. Burkenroad, Yale University’s Bingham Laboratory, carcinology and Sargasso epizoa; M. Bishop, Peabody Museum of Natural History, Zoology Dept., collections and preparations and H. Sears, WHOI ichthyologist. The itinerary included the following waypoints: Woods Hole, the Bermudas, Turks Islands, Kingston, Colon, along the Mosquito Bank off of Nicaragua, off the north coast of Jamaica, along the south coast of Cuba, Bartlett Deep, to off the Isle of Pines, through the Yucatan Channel, off Havana, off Key West, to Miami, to New York City, and then the return to Woods Hole. During the expedition, Breder collected rare and little-known flying fish species and developed a method for hatching and growing flying fish larvae. (PDF contains 48 pages)

¿Qué lee, exactamente, el que lee una traducción?

Raquel Merino Álvarez, José Miguel Santamaría, Eterio Pajares (eds.)

Charles I : unhero of royalist poetry

During the English Civil War, Charles I appeared as a character in Royalist poetry, both directly and allegorically. These depictions drew on ancient Roman epic poems, particularly Lucan’s De Bello Civili, in their treatment of the subject matter of civil war and Charles as an epic hero. Though the authors of these poems supported Charles, their depictions of him and his reign reveal anxiety about his weakness as a ruler. In comparison to the cults of personality surrounding his predecessors and the heroes of De Bello Civili, his cult appears bland and forced. The lack of enthusiasm surrounding Charles I may help to explain his downfall at the hands of his Parliamentarian opponents.