Fator de crescimento derivado de plaquetas humanas obtido por uma metodologia rápida e de baixo custo

Human platelet-derived growth factor (PDGF) was purified from lysates of clinically outdated human platelets by ionic exchange chromatography in CM-Sepharose. The eluated fraction was submitted to the Immunoblot/Slot Blot assay using anti-PDGF-AA and anti-PDGF-BB polyclonal antibodies and was evaluated as to its biological activity through the test of [H 3]-thymidine incorporation in NIH/3T3 cell line fibroblasts in culture. The Immunoblot/Slot Blot assay using anti-PDGF-AA and anti-PDGF-BB antibodies proved the presence of the PDGF in chromatographic cationic fraction. The comparison of biological activities between fiblobrast stimulation assay using recombinant PDGF-AB and partially purified PDGF was demonstrated in 165.796 and 157.567 cpm, respectively. This result, proved the potent mitogenic effect of partially purified PDGF and consequently their evidence about the wound healing activity.

Inibidores da HIV-integrase: Potencial abordagem farmacológica para tratamento da AIDS

AIDS has the HIV as its etiological agent. Researches has been done to find new pharmacological agents to be used in therapy, because of problems of resistance and side effects. The HIV-integrase inhibitors are some of those new agents that are being studied. This updating focusses on the fundamental information about HIV and HIV-integrase and the main methods being used to develop these new drugs, with examples for each case.

Comportamento do implante contendo bone morphogenetic protein (bmp) associado ao plasma rico em plaquetas na reparação de fraturas orbitárias

Purpose: This study intends to evaluate BMP (Bone Morphogenetic Protein) implant and BMP implant plus PRP (Platelet Rich Plasma) in rabbit orbital fractures, searching for tissue reaction, by radiological and morfometrical analysis. Methods:Third six white rabbits were submitted to orbital floor fracture and distributed in three groups: G1, with rabbits receiving a plate containing decalcified bone matrix and BMP; G2, with rabbits receiving the implant with BMP wrapped by PRP; G3, the control group where it was made the fracture only. The animals were evaluated radiologically after surgery and at sacrifice time in 7, 30, 90 and 180th day after surgery. After sacrifice, a block containing the right orbital tissue was extracted and prepared to morphological and morphometrical analysis. Results: An intensive linfomononuclear inflammatory reaction was observed at 7th day in G1 e G2, witch decreased after the 30th day; mesenchimal cells, osteoblasts, new bone and progressive cavitation of the implant were also observed, besides signs of calcium deposition by radiological study. In the control group fibrosis at the site of fracture was identified only. Conclusion: BMP seemed a good orbital implant producing new bone at the implant site and correcting bone defect.There was not observed acceleration of osteoinduction when the implant was associated with PRP.

Inibidores de crescimento em gramados

Well-maintained lawns are comfortable and safe places for leisure activities and sports practice, and they also bring environmental benefits; for example, they reduce soil exposure to erosion and releases atmospheric CO2, thus reducing the greenhouse effect. However, regardless of the purpose of use or the choice of the plant species to form the lawn, the highest costs involve cutting that is needed to keep the turfgrass at its appropriate height. Successive lawn cutting operations are necessary basically because of the vegetative and reproductive growth of turfgrass which, in Brazil, occurs mainly from October to March. Expenditures with successive mechanical cuttings have fostered the search of alternative procedures to keep lawn plants at appropriate height, such as the use of plant growth inhibitors, an increasingly interesting procedure. Since the use of this technology in Brazil is still at its early stage, the aim of this literature review is to examine aspects associated with lawn management by using growth inhibitors. Another alternative is to increase the knowledge of the classification and rational application of the different compounds currently available in the market.

Eficiência de herbicidas inibidores da ALS e PROTOX sob condições de déficit hídrico no comportamento bioquímico de plantas daninhas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Impacto da agregação de comportamentos de risco sobre a ocorrência de fatores de risco cardiovasculares

Pós-graduação em Fisioterapia - FCT

Modelagem molecular da interação entre a proteína de fusão do vírus sincicial respiratório humano e inibidores da ação viral. -

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Detecção in vitro da hepatite C (VHC) em plaquetas provenientes de indivíduoas não infectados expostas ao vírus

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Agregação e viés em medidas de diversidade: uma abordagem computacional intensiva

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Sistemas drug delivery aplicados a novos inibidores de topoisomerases estruturalmente derivados de toxinas bacterianas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Peptídeos inibidores de topoisomerases bacterianas: síntese e estudos de transporte através da membrana celular empregando moléculas peptídicas carreadoras

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da tolerância de leveduras a um coquetel de inibidores que simula um hidrolisado de bagaço de cana quando adicionado a um meio sintético

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Tratamento de lesões osteocondrais em joelho pela aplicação do gel de plaquetas

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Membrana transdérmica de liberação controlada utilizando quitosana, plasma e plaquetas para a regeneração de feridas

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Avaliação da resistência primária aos inibidores de integrase em pacientes soropositivos para o HIV-1

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB