Attachment to the nuclear matrix mediates specific alterations in chromatin structure

The DNA in eukaryotic chromosomes is organized into a series of loops that are permanently attached at their bases to the nuclear scaffold or matrix at sequences known as scaffold-attachment or matrix-attachment regions. At present, it is not clear what effect affixation to the nuclear matrix has on chromatin architecture in important regulatory regions such as origins of replication or the promoter regions of genes. In the present study, we have investigated cell-cycle-dependent changes in the chromatin structure of a well characterized replication initiation zone in the amplified dihydrofolate reductase domain of the methotrexate-resistant Chinese hamster ovary cell line CHOC 400. Replication can initiate at any of multiple potential sites scattered throughout the 55-kilobase intergenic region in this domain, with two subregions (termed ori-β and ori-γ) being somewhat preferred. We show here that the chromatin in the ori-β and ori-γ regions undergoes dramatic alterations in micrococcal nuclease hypersensitivity as cells cross the G1/S boundary, but only in those copies of the amplicon that are affixed to the nuclear matrix. In contrast, the fine structure of chromatin in the promoter of the dihydrofolate reductase gene does not change detectably as a function of matrix attachment or cell-cycle position. We suggest that attachment of DNA to the nuclear matrix plays an important role in modulating chromatin architecture, and this could facilitate the activity of origins of replication.

Low-cost cottage construction in America: a study based on the housing collection in the Harvard social museum

by Winthrop A. Hamlin.

Subpoena in Cocke v. Tye, 12 July 1733

Subpoena to answer a petition by James Powell Cocke. Signed by William Hamlin.

Los Angeles, California, 1908 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: City of Los Angeles, [by] Homer Hamlin, city engineer. Blueprint published in 1908. Scale [ca. 1:19,000]. The image inside the map neatline is georeferenced to the surface of the earth and fit to the California State Plane Zone V Coordinate System NAD83 (in Feet) (Fipszone 0405). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, drainage, parks, cemeteries, selected public buildings, and more. This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Rapport adressé au gouvernement d'Haïti /

Half title page : Rapport sur la Conférence Internationale Américaine de Washington.

The enjoyment of architecture,

Bibliography: p. 337-340.

The enjoyment of architecture,

Bibliography: p. 337-340.

Das illustrirte Mississippithal : dargestellt in 80 nach der Natur aufgenommenen Ansichten vom Wasserfalle zu St. Anthony an bis zum Golf von Mexico (eine Entfernung von ungefähr 2300 englischen Meilen) /

Cf. Sabin 40807.

American cases on contract : arranged in accordance with the analysis of Anson on contract ... /

Mode of access: Internet.

Annibalis Albertinii Caesenatis medici, [et] philosophi, De affectionibus cordis. Libri tres. : quorum primus agit De naturalibus. Secundus, [et] tertius, De praeternaturalibus, De palpitatione nempè, [et] syncope, atq[ue] earum curatione. In quibus multa problemata scitu dignissima enodantur, [et] praecipuè, de morborum principatu, sedeq[ue] animae, de primus morborum generibus, de Peste, ac de scopis mittendi sanguinem ....

Mode of access: Internet.

Die Angriffe der drei Barkiden auf Italien. Drei quellenkritisch-kriegsgeschichtliche Untersuchungen,

"Angeführte werke:" p. [viii]-x.

Punto per punto; dimostrazione della completa assurdeta di tutti le vecchie ipotesi intorno alla via a'Annibale, dal Rodano al Po.

Mode of access: Internet.

Annibal dans les Alpes,

On cover: Ouvrage renfermant dix-sept cartes et six photographies. Paris, A. Picard, 1902.

The Outlook

Mode of access: Internet.

The trail of the goldseekers; a record of the travel in prose and verse.

Mode of access: Internet.