(Table 1) Distribution of ostracods in Jurassic sediments of DSDP Hole 79-547

Eighteen samples from the Early Jurassic (Hettangian to Pliensbachian) and nine from the Bajocian to Berriasian interval have been examined. The ostracode fauna has been left largely in open nomenclature pending a more detailed study. At least four new genera, listed simply as Gen. nov. A-D sp. nov. have been recognized. Although many new species are present, there is a similarity between this ostracode fauna and that of northwest Europe of comparable age. This is particularly true for the Early Jurassic from which Bairdia guttulae Herrig, 1979, Ptychobairdia cf. aselfingenensis (Lord and Moorley, 1974), Monoceratina scrobiculata Triebel and Bartenstein, 1938, Bairdia sp. 4134 Michelsen, 1975, Ogmoconcha cf. contractula Triebel, 1941, and Paracypris cf. redcarensis Blake, 1876 have been obtained. Monoceratina vulsa (Jones and Sherborn, 1888), present in the Toarcian to Callovian of Britain, is recorded here from a sample provisionally dated as Bajocian to Callovian on foraminiferal evidence. The more important species are illustrated and their distribution recorded in Table 1.

Kinetic and phylogenetic characterization of an anaerobic dechlorinating microbial community

The reductive dechlorination (RD) of tetrachloroethene (PCE) to vinyl chloride (VC) and, to a lesser extent, to ethene (ETH) by an anaerobic microbial community has been investigated by studying the processes and kinetics of the main physiological components of the consortium. Molecular hydrogen, produced by methanol-utilizing acetogens, was the electron donor for the PCE RD to VC and ETH without forming any appreciable amount of other chlorinated intermediates and in the near absence of methanogenic activity. The microbial community structure of the consortium was investigated by preparing a 1 6S rDNA clone library and by fluorescence in situ hybridization (FISH). The PCR primers used in the clone library allowed the harvest of 16SrDNA from both bacterial and archaeal members in the community. A total of 616 clones were screened by RFLP analysis of the clone inserts followed by the sequencing of RFLP group representatives and phylogenetic analysis. The clone library contained sequences mostly from hitherto undescribed bacteria. No sequences similar to those of the known RD bacteria like 'Dehalococcoides ethenogenes' or Dehalobacter restrictus were found in the clone library, and none of these bacteria was present in the RD consortium according to FISH. Almost all clones fell into six previously described phyla of the bacterial domain, with the majority (56(.)6%) being deep-branching members of the Spirochaetes phylum. Other clones were in the Firmicutes phylum (18(.)5%), the Chloroflexi phylum (16(.)4%), the Bacteroidetes phylum (6(.)3%), the Synergistes genus (11(.)1%) and a lineage that could not be affiliated with existing phyla (11(.)1%). No archaeal clones were found in the clone library. Owing to the phylogenetic novelty of the microbial community with regard to previously cultured microorganisms, no specific microbial component(s) could be hypothetically affiliated with the RD phenotype. The predominance of Spirochaetes in the microbial consortium, the main group revealed by clone library analysis, was confirmed by FISH using a purposely developed probe.

Cryptic false spider mites: a new genus, Austrolinus, and a review of the family Linotetranidae (Acari : Prostigmata : Tetranychoidea)

The Linotetranidae (Acari: Tetranychoidea) is a poorly known group of cryptic false spider mites associated with grasses and sedges. We review the family at the world level, provide the first phylogenetic analysis of the family, and describe the first Australian representatives: Austrolinus, gen. nov. and two new species: A. arenulus and A. kinnearae. Linotetranidae is redefined, and keys are provided for the families of the Tetranychoidea, and for all described genera and species of Linotetranidae.

An evolutionary radiation of beeflies in semi-arid Australia: systematics of the Exoprosopini (Diptera : Bombyliidae)

Almost half of the 4822 described beeflies in the world belong to the subfamily Anthracinae, with most of the diversity found in three cosmopolitan tribes: Villini, Anthracini, and Exoprosopini. The Australian Exoprosopini previously contained three genera, Ligyra Newman, Pseudopenthes Roberts and Exoprosopa Macquart. Pseudopenthes is an Australian endemic, with two species including Ps. hesperis, sp. nov. from Western Australia. Two new species of the exoprosopine Atrichochira Hesse, Atr. commoni, sp. nov. and Atr. paramonovi, sp. nov., are also described from Australia, extending the generic distribution from Africa. Cladistic analysis clarified the phylogenetic relationships between the recognised groups of the Exoprosopini and determined generic limits on a world scale. Inclusion of 18 Australian exoprosopines placed the Australian species in the context of the world fauna. The Exoprosopini contains six large groups. The basal group I contains species previously included in Exoprosopa to which the name Defilippia Lioy is applied. Group II contains Heteralonia Rondani, Atrichochira, Micomitra Bowden, Pseudopenthes, and Diatropomma Bowden. Colossoptera Hull is newly synonymised with Heteralonia. Group III is a paraphyletic assemblage of Pterobates Bezzi and Exoprosopa including the Australian Ex. sylvana ( Fabricius). Ligyra is paraphyletic, forming two well-separated clades. The African clade is described as Euligyra Lambkin, gen. nov., which, together with Litorhina Bezzi and Hyperalonia Rondani, form group IV. The Australian group V is true Ligyra. The remaining monophyletic lineage of exoprosopines, group VI, the Balaana-group of genera, shows evidence of an evolutionary radiation of beeflies in semi-arid Australia. Phylogenetic analysis of all 42 species of the Balaana-group of genera formed a basis for delimiting genera. Seven new genera are described by Lambkin & Yeates: Balaana, Kapua, Larrpana, Munjua, Muwarna, Palirika and Wurda. Four non-Australian species belong to Balaana. Thirty two new Australian species are described: Bal. abscondita, Bal. bicuspis, Bal. centrosa, Bal. gigantea, Bal. kingcascadensis, K. corusca, K. irwini, K. westralica, Lar. collessi, Lar. zwicki, Mun. erugata, Mun. lepidokingi, Mun. paralutea, Mun. trigona, Muw. vitreilinearis, Pa. anaxios, Pa. basilikos, Pa. blackdownensis, Pa. bouchardi, Pa. cyanea, Pa. danielsi, Pa. decora, Pa. viridula, Pa. whyalla, W. emu, W. impatientis, W. montebelloensis, W. norrisi, W. patrellia, W. skevingtoni, W. windorah, and W. wyperfeldensis. The following new combinations are proposed: from Colossoptera: Heteralonia latipennis (Brunetti); from Exoprosopa: Bal. grandis (Pallas), Bal. efflatounbeyi (Paramonov), Bal. latelimbata ( Bigot), Bal. obliquebifasciata ( Macquart), Bal. tamerlan (Portschinsky), Bal. onusta ( Walker), Def. busiris (Jaennicke), Def. efflatouni ( Bezzi), Def. eritreae (Greathead), Def. gentilis ( Bezzi), Def. luteicosta ( Bezzi), Def. minos (Meigen), Def. nigrifimbriata ( Hesse), Def. rubescens ( Bezzi), K. adelaidica ( Macquart), Lar. dimidiatipennis ( Bowden), Muw. stellifera ( Walker), and Pa. marginicollis ( Gray); from Ligyra: Eu. enderleini ( Paramonov), Eu. mars ( Bezzi), Eu. monacha (Klug), Eu. paris ( Bezzi), Eu. sisyphus ( Fabricius), and Eu. venus (Karsch).

Ptychogyliauchen, a new genus of Gyliauchenidae (Platyhelminthes: Digenea) from siganid fishes of the Indo-West Pacific

We propose a new genus of the Gyliauchenidae Fukui, 1929 ( Digenea), Ptychogyliauchen, gen. nov., for four new species that infect Indo-West Pacific siganid fishes. Ptychogyliauchen, gen. nov. is a morphologically distinctive genus, diagnosed principally by the presence of a highly convoluted oesophagus, which generally exceeds the total body length of the worm, and by the unusual folded structure of the ejaculatory duct. Ptychogyliauchen thetidis, sp. nov. is designated as the type species, and is described from the intestine of Siganus punctatus (Siganidae) from Heron Island, Great Barrier Reef, Queensland, Australia. Ptychogyliauchen himinglaeva, sp. nov. is described from the intestine of Siganus corallinus ( Siganidae) from Lizard Island, Great Barrier Reef, Queensland, Australia. Ptychogyliauchen leucothea, sp. nov. is described from the intestine of S. argenteus, and further recorded from S. fuscescens, off Ningaloo, Western Australia, Australia. Ptychogyliauchen thistilbardi, sp. nov. is described from the intestine of S. doliatus from Noumea, New Caledonia, and is also found in S. argenteus, S. canaliculatus, S. corallinus and S. spinus from Noumea, New Caledonia, and Moorea, Tahiti, French Pacific. Ptychogyliauchen thistilbardi, sp. nov. also occurs in the intestine of Chaetodon citrinellus (Chaetodontidae) from Moorea. A key to species is provided. All species have been described following morphological examination using light microscopy, and specimens of P. thetidis, sp. nov., P. leucothea, sp. nov. and P. thistilbardi, sp. nov. have been characterised using molecular methods. Sequences were obtained for a combination of nuclear ribosomal (28S (D1-D3) and ITS2) and mitochondrial (ND1) genes. A phylogenetic analysis of sequenced specimens of Ptychogyliauchen, gen. nov. was conducted using species of Petalocotyle Ozaki, 1934 for outgroup comparison. This analysis, based on alignments of the ITS2 and 28S (D1-D3) rDNA genes, supports monophyly of the geographically widespread P. thistilbardi, sp. nov., which is known from both siganid and chaetodontid hosts. We discuss the taxonomy of the genus and the host associations of each species and the group.

Ankistromeces mariae n. g., n. sp (Digenea : Sanguinicolidae) from Meuschenia freycineti (Monacanthidae) off Tasmania

Ankistromeces mariae n. g., n. sp. is described from Meuschenia freycineti (Monacanthidae), the six-spined leatherjacket, from off northern Tasmania. The new genus differs from the 21 other sanguinicolid genera in the combination of the anteriorly intercaecal and posteriorly post-caecal single testis, the presence of a cirrus-sac, the absence of an auxiliary external seminal vesicle, separate genital pores, the typically post-ovarian uterus and the H-shaped intestine. A. mariae is the first sanguinicolid to be reported from a monacanthid fish.

Upper Devonian biostratigraphy of the Iberian Pyrite Belt, southwest Spain Part Two: Organic-walled microphytoplankton

Upper Devonian rocks of the Iberian Pyrite Belt (IPB) in southwest Spain, comprising the Phyllite-Quartzite Group (PQ) and the lower part of the overlying Volcano-Sedimentary Complex (VSC), contain a diversity of terrestrial and marine palynomorphs (miospores and organic-walled microphytoplankton, respectively), which constitute the basis of this biostratigraphically oriented research project. Part One of the report has previously detailed the miospore content of the constituent 117 palyniferous samples. In the present paper (i.e., the concluding Part Two), the organic-walled microphytoplankton (acritarchs and prasinophyte phycomata) are systematically described and illustrated, and their occurrence in the study material is fully documented. The acritarchs are represented by 23 species (including one species complex) allocated among 14 genera (one of which, Dupliciradiatum, is newly established), together with a very rare and novel category (informally termed Gen. nov. A). The following new acritarch species are formally instituted: Dupliciradiatum crassum (type species), D. tenue, Histopalla languida, and Winwaloeusia repagulata. Five genera allied with the prasinophycean algae are identified; these accommodate a total of 15 species of which two - Cymatiosphaera tenuimembrana and Maranhites multioculus - are formally proposed as new. In addition, representatives of the prasinophyte genera Leiosphaeridia and Tasmanites are recorded but are not discriminated at species level. The microphytoplankton suite is clearly consonant, from previously published occurrences in other regions, with a Late Devonian dating. However, most of the species are known to be relatively long ranging through (and in some cases beyond) that epoch and hence are not amenable to detailed biozonal subdivision of the IPB succession. Moreover, the distribution of the species therein tends to be erratic in comparison with the more consistently occurring miospores, possibly due to stress factors induced by fluctuating conditions in the IPBs Upper Devonian marine environment. By contrast, the land-derived (miospore) assemblages are readily applicable in a blostratigraphic context: they can be correlated precisely with the Devonian miospore biozonation scheme for Western Europe. In those terms, the sampled PQ strata are assignable to the Diducites versabilis-Grandispora cornuta (VCo) Biozone of late Famennian age; while the samples from the anoxic sequence at the base of the VSC belong to the Retispora lepidophyta-Verrucosisporites nitidus (LN) Biozone (latest Famennian = latest Devonian). The biochronostratigraphic data, in conjunction with the findings from earlier IPB studies, imply two appreciable palynostratigraphic breaks within the PQ. These are representative, respectively, of the lower Frasnian-middle Famennian interval and of part of the Strunian/upper Famennian. Speculation currently remains as to whether the inferred gaps are more apparent than real; i.e., whether one or both represent actual hiatuses in IPB sedimentation or are simply a manifestation of hitherto unsampled and/or non-palyniferous PQ strata.

Genetic uniformity of international isolates of Leifsonia xyli subsp xyli, causal agent of ratoon stunting disease of sugarcane

An international collection of the sugarcane ratoon stunting disease pathogen, Leifsonia xyli subsp. xyli, was analysed to assess genetic diversity. DNA fingerprinting using BOX primers was performed on 105 isolates, comprising 65 Australian isolates and an additional 40 isolates from Indonesia (n = 8), Japan (n = 1), USA (n = 3), Brazil (n = 2), Mali (n = 2), Zimbabwe (n = 13), South Africa (n = 9) and Reunion (n = 2). Sixty-two of these isolates were also screened using ERIC primers. No variation was found among any of the isolates. The intergenic spacer (IGS) region of the ribosomal RNA genes from 54 isolates was screened for sequence variation using single-stranded conformational polymorphism (SSCP), but none was observed. Direct sequencing of the IGS from a subset of nine isolates, representing all of the countries sampled in this study, confirmed the results of the SSCP analysis. Likewise, no sequence variation was found in the 16S ribosomal RNA genes of the same subset. Four Colombian isolates from sugarcane, morphologically similar to L. xyli subsp. xyli, were putatively shown to be an undescribed Agrococcus species of unknown pathogenicity. The lack of genetic variation among L. xyli subsp. xyli isolates, independent of time of sampling, cultivar of isolation, or country of origin, suggests the worldwide spread of a single pathogenic clone, and further suggests that sugarcane cultivars resistant to ratoon stunting disease in one area should retain this property in other regions.

Entomobryoidea e Symphypleona (Collembola, Hexapoda) em remanescentes de Mata Atlântica do Estado Do Rio Grande do Norte

Colêmbolos são pequenos artrópodes extremamente abundantes na natureza. São semelhantes a insetos em seu padrão de tagmose, ecologicamente muito importantes e podem ser usados como bioindicadores. Mais de 8.300 espécies já foram descritas no mundo, mas se estima que a riqueza real corresponda a um número muito maior. A Mata Atlântica corresponde ao domínio fitogeográfico mais alterado pela ação antrópica no Brasil, restando menos de 8,5% de sua cobertura original. No Rio Grande do Norte a Mata Atlântica cobre apenas 0,27% do território do Estado. Assim, considerando a importância de se conhecer a fauna de Collembola, sobretudo num ambiente ameaçado como a Mata Atlântica, este trabalho objetivou estudar a riqueza de colêmbolos Entomobryoidea e Symphypleona ocorrentes em dois remanescentes de Mata Atlântica do Rio Grande do Norte através de coletas ativas com aspirador entomológico e passivas com pitfall, durante os períodos de chuva e seca. Foram identificadas 22 espécies de colêmbolos, sendo que destas 19 (mais de 85% do total) são novas para a ciência, incluindo um novo gênero da família Sminthuridae. As coletas realizadas no período chuvoso apresentaram praticamente o dobro de espécies em relação às realizadas no período seco. Este fato possivelmente está associado à dependência dos colêmbolos de umidade, assim como estratégias de resistência a período secos. São descritas quatro novas espécies, incluindo o novo gênero (Trogolaphysa sp.nov., Gen.nov. sp.nov., Szeptyckitheca sp.nov. e Sminthurinus sp.nov.). Este expressivo número de espécies novas identificadas, os novos registros para o gêneros e famílias para a Mata Atlântica do Rio Grande do Norte e a descoberta de um novo gênero corroboram o pressuposto que motivou este trabalho: o Rio Grande do Norte apresenta uma fauna de Collembola desconhecida e potencialmente diversificada. Além disso, foram geradas informações importantes sobre a morfologia das espécies descritas, que por sua vez são de grande importância para a sistemática dos Collembola. O novo gênero descrito faz parte de um visível subgrupo dentro da família Sminthuridae (aqueles com o unguis com cavidade) que aparentemente tem potencial para ser reconhecido futuramente em alguma categoria taxonômica supragenérica.

Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale

Peer reviewed

Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale

Peer reviewed

Abundance of organic-walled dinoflagellate cysts in surface sediments from the Atlantic sector of the Southern Ocean (Appendix 2)

Thirty-two surface sediment samples from the Southern Ocean (eastern Atlantic sector), between the Subtropical Front and the Weddell Gyre, were investigated to provide information on the distribution of modern organic-walled dinoflagellate cysts in relation to the oceanic fronts of the Antarctic Circumpolar Current (ACC). A clearly distinguishable distribution pattern was observed in relation to the water masses and fronts of the ACC. The dinoflagellate cysts of species characteristic of open oceanic environments, such as Impagidinium species, are highly abundant around the Subtropical Front, whereas south of this front, cosmopolitan species such as Nematosphaeropsis labyrinthus and the cysts of Protoceratium reticulatum characterise the transition from subtropical to subantarctic surface waters. The subantarctic surface waters are dominated by the cysts of heterotrophic dinoflagellates, such as Protoperidinium spp. and Selenopemphix antarctica. The cysts of Protoperidinium spp. form the dominant part of the assemblages around the Antarctic Polar Front, whereas S. antarctica concentrations increase further to the south. The presence of S. antarctica in sediments of the Maud Rise, a region of seasonal sea-ice cover, reflects its tolerance for low temperatures and sea-ice cover. A previously undescribed species, Cryodinium meridianum gen. nov. sp. nov., has a restricted distribution pattern between the Antarctic Polar Front and the ACC-Weddell Gyre Boundary.

Distribution of desmoscolecida (Nematoda) in surface sediments of the Iberian Deep-Sea, North Atlantic (Table 1)

1. Desmoscolecida from the continental slope and the deep-sea bottom (59-4354 m) off the Portuguese and Moroccan coasts are described. 18 species were identified: Desmoscolex bathyalis sp. nov., D. chaetalatus sp. nov., D. eftus sp. nov., D. galeatus sp. nov., D. lapilliferus sp. nov., D. longisetosus Timm, 1970, D. lorenzeni sp. nov., D. perspicuus sp. nov., D. pustulatus sp. nov., Quadricoma angulocephala sp. nov., Q. brevichaeta sp. nov., Q. iberica sp. nov., Q. loricatoides sp. nov., Tricoma atlantica sp. nov., T. bathycola sp. nov., T. beata sp. nov., T. incomposita sp. nov., T. meteora sp. nov., T. mauretania sp. nov. 2. The following new terms are proposed: "Desmos" (ring-shaped concretions consisting of secretion and concretion particles), "desmoscolecoid" and "tricomoid" arrangement of the somatic setae, "regelmaessige" (regular), "unregelmaessige" (irregular), "vollstaendige" (complete) and "unvollstaendige" (incomplete) arrangement of somatic seta (variations in the desmoscolecoid arrangement of the somatic setae). The length of the somatic setae is given in the setal pattern. 3. Desmoscolecida identical as to genus and species exhibit no morphological differences even if forthcoming from different bathymetrical zones (deep sea, sublitoral, litoral) or different environments (marin, freshwater, coastal subsoil water, terrestrial environment). 4. Lorenzen's (1969) contention that thearrangement of the somatic setae is more significant for the natural relationships between the different genera of Desmoscolecida than other characteristics is further confirmed. Species with tricomoid arrangement of somatic setae are regarded as primitive, species with desmoscolecoid arrangement of somatic setae are regarded as more advanced. 5. Three new genus are established: Desmogerlachia gen. nov., Desmolorenzenia gen. nov. and Desmofimmia gen. nov. - Protricoma Timm, 1970 is synonymized with Paratricoma Gerlach, 1964 and Protodesmoscolex Timm, 1970 is synonymized with Desmoscolex Claparede,1863. 6. Checklists of all species of the order Desmoscolecida and keys to species of the subfamilies Tricominae and Desmoscolecinae are provided. 7. The following nomenclatorial changes are suggested: Desmogerlachia papillifer (Gerlach, 1956) comb. nov., D .pratensis (Lorenz, 1969) comb. nov., Desmotimmia mirabilis (Timm, 1970) comb. nov., Paratricoma squamosa (Timm, 1970) comb. nov., Desmolorenzenia crassicauda (Timm, 1970) comb. nov., D. desmoscolecoides (Timm, 1970) comb. nov., D. eurycricus (Filipjev, 1922) comb. nov., D. frontalis (Gerlach, 1952) comb. nov., D. hupferi (Steiner, 1916) comb. nov., D. longicauda (Timm, 1970) comb. nov., D. parva (Timm, 1970) comb. nov., D. platycricus (Steiner, 1916) comb. nov., D. viffata (Lorenzen, 1969) comb. nov., Desmoscolex anfarcficos (Timm, 1970) comb. nov.

First Triassic tetrapod (Sauropterygia, Nothosauridae) from Castilla y León: evidence of an unknown taxon for the Spanish record

Several vertebrae of a sauropterygian specimen have been recovered in Fuencaliente de Medinaceli (Soria Province, Castilla y León, Spain). The remains come from Middle–Upper Triassic Muschelkalk Facies. This finding represents the first documented evidence of a Triassic tetrapod in Castilla y León. The vertebrae belong to Nothosaurus, a sauropterygian genus found in Europe, Middle East, North of Africa and China. This genus is poorly-known in the Iberian record. The new remains constitute the first evidence of the species Nothosaurus giganteus, or a related taxon, in the Iberian Peninsula. This study reveals the occurrence of at least two species of the sauropterygian Nothosaurus in the Spanish record.

Systematics of African Amphicyonidae, with descriptions of new material from Napak (Uganda) and Grillental (Namibia)

The Early Miocene Napak XV locality (ca 20.5 Ma), Uganda, has yielded an interesting assemblage of fossils, including the very well represented amphicyonid Hecubides euryodon. The remarkable find of a nearly complete mandible, unfortunately with poorly preserved dentition, together with new dental remains allow us to obtain a better idea about the morphology and variability of this species. Additionally, we describe a newly discovered mandible of Hecubides euryodon from the Grillental-VI locality (Sperrgebiet, Namibia), which is the most complete and diagnostic Amphicyonidae material found in this area. Comparisons with Cynelos lemanensis from Saint Gérand le Pouy (France), the type locality, and with an updated sample of the species of amphicyonids described in Africa leads us to validate the genus Hecubides. Hecubides would be phylogenetically related to the medium and large size species of Amphicyonidae from Africa, most of them now grouped into the genera Afrocyon and Myacyon, both endemic to this continent.