weathering fur simulation

ACM SIGGRAPH; ACM SIGCHI

Cys-92, Cys-95, and the C-terminal 12 residues of the Vibrio harveyi ferric uptake regulator (Fur) are functionally inessential

Ferric uptake regulator (Fur) is a global regulator involved in multiple aspects of bacterial life. The gene encoding the Vibrio harveyi Fur (Fur(vh)) was cloned from a pathogenic V. harveyi strain isolated from diseased fish. Furvh shares 77% overall sequence identity with the Escherichia coli Fur (Fur(Ec)) and could complement a mutant of Fur(Ec). Like Fur(Ec), Fur(Vh), possesses two cysteine residues at positions 92 and 95, yet unlike Fur(Ec), in which these cysteine residues constitute part of the metal ion coordination site and hence are vital to the repressor activity, C92 and C95 of Fur(Vh) proved to be functionally inessential. Further study identified a Vibrio Fur signature sequence, which is preserved in all the ten Vibrio Fur proteins that have been discovered to date but in none of the non-vibrio Fur proteins. Site-directed and random mutation analyses of the signature residues, the cysteine residues, and seven highly charged amino acid residues indicated that D9, H32, C137, and K138 of Fur(vh) are functionally important but D9, C137, and K138 can be replaced by more than one functional substitutes. Systematic deletion analysis demonstrated that the C-terminal 12 residues of Fur(Vh) are functionally inessential. These results (i) indicated that the activation mechanism, or certain aspects of which, of Fur(Vh) is possibly different from that of Fur(Ec); and (ii) suggested that it is not very likely that the C-terminal 12 residues play any significant role in the activation or stability of Fur(Vh); and (iii) provided insights into the potential function of the local structure involving C137 and K138.

Molecular analysis of the fur (ferric uptake regulator) gene of a pathogenic Edwardsiella tarda strain

The gene encoding the Edwardsiella tarda ferric uptake regulator (Fur(Et)) was cloned from a pathogenic E. tarda strain isolated from diseased fish. Fur(Et) shares 90% overall sequence identity with the Escherichia coli Fur (Fur(Ec)) and was able to complement the mutant phenotype of a fur(Ec)-defective E. coli strain. Mutational analysis indicated that C92S and C95S mutations inactivated Fur(Et) whereas E112K mutation resulted in a superactive Fur(Et) variant. Fur(Et) negatively regulated its own expression; interruption of this regulation impaired bacterial growth, altered the production of certain outer membrane proteins, and attenuated bacterial virulence.

A Christian analytic approach of marriage through abduction among the Basotho people of Lesotho: A challenge for pastoral care ministry

University of Pretoria / MA Dissertation / Department of Practical Theology / Advised by Prof M J S Masango

A Christian Reading of History

This recording is part of the Marsh Chapel Audio Collection.

Christian missions ; their agents, and their results / by T.W.M. Marshall.

http://www.archive.org/details/a592254601marsuoft

Christian missions : their agents and their results (vol. 1) / by T.W.M. Marshall.

http://books.google.com/books?vid=ISBN0665456816&id=sipohllLjKQC&dq=protestant+missions&a_sbrr=1 View book via Google

Christian missions and a new world culture

http://www.archive.org/details/christianmission027881mbp

Christian missions and social progress : a sociological study of foreign missions (vol. 1)

http://www.archive.org/details/socialprogress00dennuoft/

Christian missions : their agents and their results (vol. 2) / by T.W.M. Marshall.

http://books.google.com/books?vid=ISBN0665456816&id=sipohllLjKQC&dq=protestant+missions&a_sbrr=1 View book via Google

The Christian mission in America

http://www.archive.org/details/christianmission028099mbp

Christian cooperation in Latin America

http://www.archive.org/details/christiancoopera00inmauoft/

The child in the midst : a comparative study of child welfare in Christian and non-Christian lands / by Mary Schauffler Labaree.

http://www.archive.org/details/childinthemidst028451mbp

A Chinese appeal to Christendom concerning Christian mission / by Lin Shao-yang [pseud.].

http://www.archive.org/details/achineseappealto00johnuoft