The Basilewsky Situla, top row, Crucifixion, and Judas the money to the High Priest. bottom row, Christ appearing to the Apostles, and the Incredulity of St Thomas

4 17/32 in.x 1 ft. 3 55/64 in.x 6 19/64 in.; carved ivory

How can the health care of United States citizens best be improved? : a collection of excerpts and bibliography relating to the high school debate topic, 1977-1978, pursuant to Public Law 88-246 /

Mode of access: Internet.

The trials of James, Duncan and Robert M'Gregor, three sons of the celebrated Rob Roy, before the High court of justiciary, in the years 1752, 1753, and 1754. To which is prefixed a memoir relating to the Highlands, with anecdotes of Rob Roy and his family.

Trials for the abduction of Jean Key Wright.

A guide to the magistracy and lieutenancy of Berks : the acreage and population of the county, the petty sessional divisions, the times and places of holding, and the order of proceeding at the quarter sessions. To which is added an account of the lords-lieutenant, the militia, and yeomanry of the county and also lists of the high sheriffs and of the members returned to Parliament ...

Mode of access: Internet.

Gospel themes; a treatise on salient features of "Mormonism," written for, and dedicated to the High Priests, Seventies and Elders of the Church of Jesus Christ of L.D.S.

Mode of access: Internet.

Review of the Mayor's Report, on the subject of schools : so far as it relates to the High School for Girls /

Reviews "the report of the Mayor, in behalf 'of a sub-committee of the School Committee, recommending ... various improvements in the system of instruction in the grammar and writing schools of this city,'" written by the chairman of the School Committee, Josiah Quincy--P. [3].

The importance of the last promise of Jesus Christ to Christian missionaries. A sermon, delivered at Springfield, May 10, 1826, at the ordination of the Rev. Rufus Anderson, as an evangelist, and of the Rev. Messrs. Josiah Brewer, Eli Smith, Cyrus Stone, and Jeremiah Stow, to the high and sacred office of Christian missionaries. ...

Printed buff paper covers.

A complete collection of the treaties and conventions, and reciprocal regulations at present subsisting between Great Britain and foreign powers ... so far as they relate to commerce and navigation; and to the repression and abolition of the slave trade; and to the privileges and interests of the subjects of the high contracting parties.

List of compilers. Vol.1-11, Lewis Hertslet. Vol.12-16, Edward Hertslet. Vol.17-19, Edward Hertslet and Cecil Hertslet. Vol.20, Augustus H.Oakes and F.H.T. Streatfield. Vol.21-23, Augustus H. Oakes and Richard W.Brant. Vol.24-25, Richard W.Brant and G.E.P. Hertslet. Vol.26, G.E.P. Hertslet and Edward Parkes. Vol.27, Edward Parkes and W.L. Berrow. Vol.28, C.S. Nicoll and W.L. Berrow and J.W. Field. Vol.29-30, C.S. Nicoll and W.L. Berrow.

Hertslet's commercial treaties. A collection of treaties and conventions, between Great Britain and foreign powers, and of the laws, decrees, orders in council, &c., concerning the same, so far as they relate to commerce and navigation, slavery, extradition, nationality, copyright, postal matters, &c., and to the privileges and interests of the subjects of the high contracting parties.

At head of title, v. 30-31: Foreign office.

The integration of ProxiMAX randomisation with CIS display for the high-throughput production of novel peptides

Saturation mutagenesis is a powerful tool in modern protein engineering. This can allow the analysis of potential new properties thus allowing key residues within a protein to be targeted and randomised. However, the creation of large libraries using conventional saturation mutagenesis with degenerate codons (NNN or NNK) has inherent redundancy and disparities in residue representation. In this we describe the combination of ProxiMAX randomisation and CIS display for the use of generating novel peptides. Unlike other methods ProxiMAX randomisation does not require any intricate chemistry but simply utilises synthetic DNA and molecular biology techniques. Designed ‘MAX’ oligonucleotides were ligated, amplified and digested in an iterative cycle. Results show that randomised ‘MAX’ codons can be added sequentially to the base sequence creating a series of randomised non-degenerate codons that can subsequently be inserted into a gene. CIS display (Isogencia, UK) is an in vitro DNA based screening method that creates a genotype to phenotype link between a peptide and the nucleic acid that encodes it. The use of straight forward in vitro transcription/translation and other molecular biology techniques permits ease of use along with flexibility making it a potent screening technique. Using ProxiMAX randomisation in combination with CIS display, the aim is to produce randomised anti-nerve growth factor (NGF) and calcitonin gene-related (CGRP) peptides to demonstrate the high-throughput nature of this combination.