(Table 1) Results from XRD analysis as well as sediment type and selected results from ring shear experiments

The location of the seaward tip of a subduction thrust controls material transfer at convergent plate margins, and hence global mass balances. At approximately half of those margins, the material of the subducting plate is completely underthrust so that no accretion or even subduction erosion takes place. Along the remaining margins, material is scraped off the subducting plate and added to the upper plate by frontal accretion. We here examine the physical properties of subducting sediments off Costa Rica and Nankai, type examples for an erosional and an accretionary margin, to investigate which parameters control the level where the frontal thrust cuts into the incoming sediment pile. A series of rotary-shear experiments to measure the frictional strength of the various lithologies entering the two subduction zones were carried out. Results include the following findings: (1) At Costa Rica, clay-rich strata at the top of the incoming succession have the lowest strength (µres = 0.19) while underlying calcareous ooze, chalk and diatomite are strong (up to µres = 0.43; µpeak = 0.56). Hence the entire sediment package is underthrust. (2) Off Japan, clay-rich deposits within the lower Shikoku Basin inventory are weakest (µres = 0.13-0.19) and favour the frontal proto-thrust to migrate into one particular horizon between sandy, competent turbidites below and ash-bearing mud above. (3) Taking in situ data and earlier geotechnical testing into account, it is suggested that mineralogical composition rather than pore-pressure defines the position of the frontal thrust, which locates in the weakest, clay mineral-rich (up to 85 wt.%) materials. (4) Smectite, the dominant clay mineral phase at either margin, shows rate strengthening and stable sliding in the frontal 50 km of the subduction thrust (0.0001-0.1 mm/s, 0.5-25 MPa effective normal stress). (5) Progressive illitization of smectite cannot explain seismogenesis, because illite-rich samples also show velocity strengthening at the conditions tested.

Simple In Vitro Assay for Determining the Sensitivity of Plasmodium vivax Isolates from Fresh Human Blood to Antimalarials in Areas where P. vivax Is Endemic

The aim of this study was to develop a simple, field-practical, and effective in vitro method for determining the sensitivity of fresh erythrocytic Plasmodium vivax isolates to a range of antimalarials. The method used is a modification of the standard World Health Organization (WHO) microtest for determination of P.falciparum drug sensitivity. The WHO method was modified by removing leukocytes and using a growth medium supplemented with AB(+) serum. We successfully carried out 34 in vitro drug assays on 39 P. vivax isolates collected from the Mae Sod malaria clinic, Tak Province, Thailand. The mean percentage of parasites maturing to schizonts (six or more merozoites) in control wells was 66.5% +/- 5.9% (standard deviation). This level of growth in the control wells enabled rapid microscopic determination (5 min per isolate per drug) of the MICs of chloroquine, dihydroartemisinin, WR238605 (tafenoquine), and sulfadoxine. P. vivax was relatively sensitive to chloroquine (MIC = 160 ng/ml, 50% inhibitory concentration [IC50] = 49.8 ng/ml) and dihydroartemisinin (MIC = 0.5 ng/ml, IC50 = 0.47 ng/ml). The poor response of P. vivax to both tafenoquine (MIC = 14,000 ng/ml, IC50 = 9,739 ng/ml) and sulfadoxine (MIC = 500,000 ng/ml, IC50 = 249,000 ng/ml) was due to the slow action of these drugs and the innate resistance of P. vivax to sulfadoxine. The in vitro assay developed in our study should be useful both for assessing the antimalarial sensitivity of P. vivax populations and for screening new antimalarials in the absence of long-term P. vivax cultures.

Ocular biotribology and contact lens lubrication mechanisms

The work described in this thesis is concerned with mechanisms of contact lens lubrication. There are three major driving forces in contact lens design and development; cost, convenience, and comfort. Lubrication, as reflected in the coefficient of friction, is becoming recognised as one of the major factors affecting the comfort of the current generation of contact lenses, which have benefited from several decades of design and production improvements. This work started with the study of the in-eye release of soluble macromolecules from a contact lens matrix. The vehicle for the study was the family of CIBA Vision Focus® DAILIES® daily disposable contact lenses which is based on polyvinyl alcohol (PVA). The effective release of linear soluble PVA from DAILIES on the surface of the lens was shown to be beneficial in terms of patient comfort. There was a need to develop a novel characterisation technique in order to study these effects at surfaces; this led to the study of a novel tribological technique, which allowed the friction coefficients of different types of contact lenses to be measured reproducibly at genuinely low values. The tribometer needed the ability to accommodate the following features: (a) an approximation to eye lid load, (b) both new and ex-vivo lenses, (c) variations in substrate, (d) different ocular lubricants (including tears). The tribometer and measuring technique developed in this way was used to examine the surface friction and lubrication mechanisms of two different types of contact lenses: daily disposables and silicone hydrogels. The results from the tribometer in terms of both mean friction coefficient and the friction profiles obtained allowed various mechanisms used for surface enhancement now seen in the daily disposable contact lens sector to be evaluated. The three major methods used are: release of soluble macromolecules (such as PVA) from the lens matrix, irreversible surface binding of a macromolecule (such as polyvinyl pyrrolidone) by charge transfer and the simple polymer adsorption (e.g. Pluoronic) at the lens surface. The tribological technique was also used to examine the trends in the development of silicone hydrogel contact lenses. The focus of the principles in the design of silicone hydrogels has now shifted from oxygen permeability, to the improvement of surface properties. Presently, tribological studies reflect the most effective in vitro method of surface evaluation in relation to the in-eye comfort.

Transversal and longitudinal images from the retina of the living eye using low coherence interferometry

An optical coherence tomography (OCT) system to produce both longitudinal and transversal images of the in vivo human eye is presented. For the first time, OCT transversal images collected from the living eye at 50-µm depth steps show details unobtainable with the state-of-the-art scanning laser ophthalmoscope. Images of up to 3×3?mm are produced from the retina in less than a second. For images larger than 1.6×1.6?mm, a path modulation is introduced by the galvanometric scanning mirror and is used as an effective phase modulation method.

Transversal and longitudinal images from the retina of the living eye using low coherence interferometry

An optical coherence tomography (OCT) system to produce both longitudinal and transversal images of the in vivo human eye is presented. For the first time, OCT transversal images collected from the living eye at 50-µm depth steps show details unobtainable with the state-of-the-art scanning laser ophthalmoscope. Images of up to 3×3?mm are produced from the retina in less than a second. For images larger than 1.6×1.6?mm, a path modulation is introduced by the galvanometric scanning mirror and is used as an effective phase modulation method.

Improved response time of laser etched polymer optical fiber Bragg grating humidity sensor

The humidity sensor made of polymer optical fiber Bragg grating (POFBG) responds to the water content change in fiber induced by the change of environmental condition. The response time strongly depends on fiber size as the water change is a diffusion process. The ultra short laser pulses have been providing an effective micro fabrication method to achieve spatial localized modification in materials. In this work we used the excimer laser to create different microstructures (slot, D-shape) in POFBG to improve its performance. A significant improvement in the response time has been achieved in a laser etched D-shaped POFBG humidity sensor.

Modelling the growth of young individuals of Pinus nigra on thin carbonate soils under climate change in Hungary

Climate change highly impacts on tree growth and also threatens the forest of the karstic terrains. From the 1980s the frequency of decay events of the Pinus nigra Arnold forests showed a marked increase in Hungary. To understanding the vulnerability of Pinus nigra forests to climate change on shallow karstic soils in continental-sub Mediterranean climatic conditions we developed the study of three sampled population in the typical karstic landscape of Veszprém in North Transdanubia. We built our model on non-invasive approach using the annual growth of the individuals. MPI Echam5 climate model and as aridity index the Thornthwaite Agrometeorological Index were used. Our results indicate that soil thickness up to 11 cm has a major influence on the main growth intensity, however, aridity determines the annual growth rate. Our model results showed that the increasing decay frequency in the last decades was a parallel change to the decreasing growth rate of pines. The climate model predicts the similar, increased decay frequency to the presents. Our results can be valid for a wider areas of the periphery of Mediterranean climate zone while the annual-growth based model is a cost-effective and simple method to study the vitality of pine trees in a given area.

Drying Times: Integrating Citizen Science to Examine Survival of Florida Largemouth Bass in a Coastal Refuge Habitat

In aquatic systems refuge habitats increase resistance to drying events and are necessary for maintaining populations in disturbed environments. However, reduced water availability and altered flow regimes threaten the existence and function of these habitats. To test refuge function I conducted a capture-mark-recapture (CMR) study, integrating citizen science angler sampling into fisheries-independent methods. The objectives of this study were twofold: 1) To determine the contribution of citizen science anglers to improving CMR research, and 2.) to quantify apparent survival of Florida Largemouth Bass, Micropterus salmoides floridanus, in a coastal refuge habitat across multiple years of drying severity. The inclusion of angler sampling was determined to be an effective and feasible method for increasing capture probability. Apparent survival of Florida Bass varied among hydrologic periods with lowest survival when marshes functionally dried (< 10 cm). Overall mortality from drying events increased with the duration of marsh drying upstream.

Swellable polymer films containing Au nanoparticles for point-of-care therapeutic drug monitoring using surface-enhanced Raman spectroscopy

Large (10 × 10 cm) sheets of surface-enhanced Raman spectroscopy (SERS) active polymer have been prepared by stabilising metal nanoparticle aggregates within dry hydroxyethylcellulose (HEC) films. In these films the aggregates are protected by the polymer matrix during storage but in use they are released when aqueous analyte droplets cause the films to swell to their gel form. The fact that these "Poly-SERS" films can be prepared in bulk but then cut to size and stored in air before use means that they provide a cost effective and convenient method for routine SERS analysis. Here we have tested both Ag and Au Poly-SERS films for use in point-of-care monitoring of therapeutic drugs, using phenytoin as the test compound. Phenytoin in water could readily be detected using Ag Poly-SERS films but dissolving the compound in phosphate buffered saline (PBS) to mimic body fluid samples caused loss of the drug signal due to competition for metal surface sites from Cl^- ions in the buffer solution. However, with Au Poly-SERS films there was no detectable interference from Cl^- and these materials allowed phenytoin to be detected at 1.8 mg L^-1, even in PBS. The target range of detection of phenytoin in therapeutic drug monitoring is 10-20 mg L^-1. With the Au Poly-SERS films, the absolute signal generated by a given concentration of phenytoin was lower for the films than for the parent colloid but the SERS signals were still high enough to be used for therapeutic monitoring, so the cost in sensitivity for moving from simple aqueous colloids to films is not so large that it outweighs the advantages which the films bring for practical applications, in particular their ease of use and long shelf life.

Cyberbullying: estudo da prevalência de comportamentos de cyberbullying e sua relação com vivências de vergonha e estados emocionais negativos

Objectivos: O presente estudo tem como principal objectivo avaliar a prevalência dos comportamentos de cyberbullying, analisando a influência de variáveis sóciodemográficas, e, compreender a sua relação com as vivências de vergonha interna e externa e com os estados emocionais negativos, particularmente a depressão, a ansiedade e o stress. Método: Para a recolha de dados recorreu-se a uma mostra de adolescentes (N=131) a frequentar o 3º ciclo do ensino básico, com idades compreendidas entre os 12 e os 18 anos (M= 13,76; DP= 1,25). O protocolo de avaliação foi constituído por um questionário psicossocial desenvolvido especificamente para este estudo e por um conjunto de medidas fidedignas para avaliar o cyberbullying (CBQ e CBQ-V), a vergonha interna (ISS), vergonha externa (OAS) e os estados emocionais negativos (DASS-21). Resultados: Os nossos dados revelaram que 76 adolescentes (58%) exerceram um qualquer comportamento de cyberbullying (com um predomínio dos rapazes), enquanto 50 adolescentes (38,2%) já foram vítimas de um qualquer comportamento de cyberbullying (com igual proporção entre rapazes e raparigas). Manter lutas e discussões online, usando insultos mediante mensagens electrónicas foi o comportamento praticado mais frequente (30,5%), enquanto o ser removido intencionalmente de um grupo online foi o comportamento sofrido mais frequente (16,1%). A idade e os anos de reprovações mostraram uma associação positiva com os comportamentos de agressão por cyberbullying. Foi ainda analisada a sobreposição entre a execução e a vitimização de comportamentos de cyberbullying, tendo sido discriminados quatro grupos de adolescentes: só agressores (adolescentes que apenas exerceram comportamentos de agressão), só vítimas (apenas sofreram comportamentos de cyberbulling), vítimas e agressores (adolescentes que são simultaneamente agressores e vítimas), e nem vítimas nem agressores (adolescentes que não exerceram nem sofreram qualquer comportamento de cyberbullying). Os resultados evidenciaram que quanto maior a frequência de comportamentos de agressão por cyberbullying, maior a vergonha interna e maior os níveis de stress demonstrados. Por sua vez, quanto maior a frequência de vitimização por cyberbullying, maior a vergonha interna e externa, bem como maior os níveis de ansiedade e stress. Conclusão: Devido a complexidade do fenómeno cyberbullying e seu recente surgimento, serão necessários mais estudos, particularmente longitudinais, para compreender a relação antecedente e/ou consequentes aos comportamentos de cyberbullying entre estados emocionais negativos e as experiências de vergonha. / Objectives: The present study has as main objective to assess the prevalence of cyberbullying behaviours, analyzing the influence of socio demographic variables, and, understand its relationship to the experiences of internal and external shame and negative emotional states, particularly depression, anxiety and stress. Method: For data collection we used a sample of adolescents (N=131) attending the 3rd cycle of basic education, aged between 12 and 18 years (M=13,76; SD=1,25). The evaluation protocol consisted of a psychosocial questionnaire developed specifically for this study and a set of reliable measure to assess cyberbullying (CBQ and CBQ-V), internal shame (ISS), external shame (OAS) and the emotional states negative (DASS-21). Results: Our data indicate that 76 adolescents (58%) exerted any conduct of a cyberbullying (with a predominance of boys), while 50 adolescents (38,2%) had been victims of cyberbullying behaviour of any one (with an equal ratio of boys and girls). Keep fighting and discussions online, through e-mails using insults behaviour was practiced more often (30,5%), while being intentionally removed a group of online behaviour is seen more frequently (16,1%). The age and years of failures were positively associated with the behaviours of aggression by cyberbullying. Was further examined the overlap between enforcement and victimization of cyberbullying behaviours, having been discriminated four groups of adolescents: only aggressors (adolescents who have had only aggressive behaviour), only victims (only suffered cyberbullying behaviours), victims and aggressors (adolescents who are both perpetrators and victims), and neither victims nor aggressors (adolescents who did not exercise any behaviour or suffered cyberbullying). Results showed that the higher the frequency of aggression by cyberbullying behaviour, the greater shame and internal stress levels demonstrated. In turn, the higher the frequency of cyberbullying victimization, the greater the shame internal and external, as well as higher levels of anxiety and stress. Conclusion: Due to the complexity of the phenomenon cyberbullying and its recent emergence, further studies are needed, particularly longitudinal, to understand the relationship between antecedent and/or consequential to cyberbullying behaviours between negative emotional states and experiences of shame.

Researching a segmented market: reflections on telephone interviewing

Purpose The purpose of this paper was to review the effectiveness of telephone interviewing for capturing data and to consider in particular the challenges faced by telephone interviewers when capturing information about market segments. Design/methodology/approach The platform for this methodological critique was a market segment analysis commissioned by Sport Wales which involved a series of 85 telephone interviews completed during 2010. Two focus groups involving the six interviewers involved in the study were convened to reflect on the researchers’ experiences and the implications for business and management research. Findings There are three principal sets of findings. First, although telephone interviewing is generally a cost-effective data collection method, it is important to consider both the actual costs (i.e. time spent planning and conducting interviews) as well as the opportunity costs (i.e. missed appointments, “chasing participants”). Second, researchers need to be sensitised to and sensitive to the demographic characteristics of telephone interviewees (insofar as these are knowable) because responses are influenced by them. Third, the anonymity of telephone interviews may be more conducive for discussing sensitive issues than face-to-face interactions. Originality/value The present study adds to this modest body of literature on the implementation of telephone interviewing as a research technique of business and management. It provides valuable methodological background detail about the intricate, personal experiences of researchers undertaking this method “at a distance” and without visual cues, and makes explicit the challenges of telephone interviewing for the purposes of data capture.

Prepuce-sparing plasty and simple running suture for phimosis

Aim. Circumcision is the most common procedure for phimosis. In recent years, the value of foreskin properties as well as aesthetic reasons determined to other operations preserving the foreskin. We report 5-years experience with a technique that preserve the physical foreskin appearance intact. Patients and methods. Fifty-two patients, eligible to undergo phimosis surgery, underwent prepuce-sparing plasty and simple running suture. Evaluation of results was made with photos comparative and verified by using presence/absence of recurrence, scarring evaluation, and VAS for patient satisfaction. Results. Forty-eight patients reported no complications. There were no cases of bleeding, infection, pathological scarring, phimosis recurrence. The scar showed a good pliability and a thin thickness. Patient satisfaction was high. Conclusions. The association of prepuce-sparing plasty and simple running suture highlighted an effective and easy method for the correction of acquired phimosis in adult patients, with excellent functional and cosmetic results.

Development of an historical landscape photography database to support landscape change analysis in the Northeast of Portugal

Repeat photography is an efficient, effective and useful method to identify trends of changes in the landscapes. It was used to illustrate long-term changes occurring in the landscapes. In the Northeast of Portugal, landscapes changes is currently driven mostly by agriculture abandonment and agriculture and energy policy. However, there is a need to monitoring changes in the region using a multitemporal and multiscale approach. This project aimed to establish an online repository of oblique digital photography from the region to be used to register the condition of the landscape as recorded in historical and contemporary photography over time as well as to support qualitative and quantitative assessment of change in the landscape using repeat photography techniques and methods. It involved the development of a relational database and a series of web-based services using PHP: Hypertext Preprocessor language, and the development of an interface, with Joomla, of pictures uploading and downloading by users. The repository will make possible to upload, store, search by location, theme, or date, display, and download pictures for Northeastern Portugal. The website service is devoted to help researchers to obtain quickly the photographs needed to apply RP through a developed search engine. It can be accessed at: http://esa.ipb.pt/digitalandscape/.

A randomised controlled trial comparing weight adjusted dose versus fixed dose prophylactic phenylephrine infusion on maintaining systolic blood pressure during caesarean section under spinal anaesthesia.

Background: Spinal anaesthesia is the standard of care for elective caesarean delivery. It has advantages over general anaesthesia. However the sympathetic blockade induced by spinal anaesthesia results in an 80 percent incidence of hypotension without prophylactic management. Current evidence supports co-loading with intravenous fluids in conjunction with the use of vasopressors as the most effective way to prevent and treat the hypotension. Phenylephrine is the accepted vasopressor of choice in the parturient. A prophylactic phenylephrine infusion combined with a fluid co-load is proven to be an effective and safe method of maintaining maternal hemodynamic stability. While most published studies have assessed the effectiveness of a prophylactic phenylephrine fixed dose infusion, few studies have assessed the effect of a prophylactic phenylephrine weight adjusted dose infusion on maintaining maternal hemodynamic stability following spinal anesthesia for a cesarean delivery. Objective: To compare the incidence of hypotension between women undergoing elective caesarean section under spinal anaesthesia, receiving prophylactic phenylephrine infusion at a fixed dose of 37.5 micrograms per minute versus a weight adjusted dose of 0.5 micrograms per kilogram per minute. Methods: One hundred and eight patients scheduled for non-urgent caesarean section under spinal anaesthesia were randomized into 2 groups; control group and intervention group using a computer generated table of numbers. Control group; Received prophylactic phenylephrine fixed dose infusion at 37.5 micrograms per minute. Intervention group; Received prophylactic phenylephrine weight adjusted dose infusion at 0.5 micrograms per kilogram per minute Results: The two groups had similar baseline characteristics in terms of ; Age, sex, weight and height. There was a 35.2% incidence of hypotension in the fixed dose group and an 18.6% incidence of hypotension in the weight adjusted dose group. This difference was found to be of borderline statistical significance p-value 0.05, and the difference in the incidence rates between the two groups was found to be statistically significant p= 0.03. The difference in the incidence of reactive hypertension and bradycardia between the two groups was not statistically significant: p-value of 0.19 for reactive hypertension and p-value of 0.42 for the incidence of bradycardia. There was also no statistically significant difference in the use of phenylephrine boluses, use of atropine, intravenous fluid used and the number of times the infusion was stopped. Conclusion: Among this population, the incidence of hypotension was significantly less in the weight adjusted dose group than in the fixed dose group. There was no difference in the number of physician interventions required to keep the blood pressure within 20% of baseline, and no difference in the proportion of reactive hypertension or bradycardia between the two groups. Administering prophylactic phenylephrine infusion at a weight adjusted dose of 0.5 micrograms per kilogram per minute results in a lower incidence of hypotension compared to its administration at a fixed dose of 37.5 micrograms per minute.