Endoscopic approach for a laryngeal neoplasm in a dog

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Use of Guanidine Compounds in the Treatment of Neglected Tropical Diseases

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Is rainfall seasonality important for reproductive strategies in viviparous Neotropical pit vipers? A case study with Bothrops leucurus from the Brazilian Atlantic Forest

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização estrutural do Lineamento de Piúma e sua influência na porção norte da Bacia de Campos

The detailed study of the Brazilian continental margin basins became possible with the advancement of geophysical tools, with emphasis on seismic reflection. Characterizing the structural and stratigraphic elements of Brazil’s marginal basins they realized that there was a relationship with structures present on the adjacent continental basement. So many works began to be made to understand this relationship and know the major factors that influenced the evolution of the continental margin. The study area of this work includes the northern portion of the Campos Basin and the continental outcropping adjacent areas, which corresponds to the northern of Rio de Janeiro state and the southern of Espirito Santo state. This area stands an important structural feature of NW-SE direction with a projection to the Campos Basin called Lineamento de Piúma. The outcropping basement rocks belongs to the Ribeira Belt which was bonded to other mobile bands forming the continent Western Gondwana during the Brasiliano Cycle, which later fragmented giving rise to the Atlantic Ocean. The opening of the ocean results on the formation of the marginal basins of Brazil. These basins have continental, transitional and marine facies. On Campos Basin the continental phase resulted on the formation of horsts and grabens bounded by synthetic and antithetic faults. Continuing rifting formed the saline lakes that deposited siliciclastic and carbonate sediments. The transitional phase resulted on thick packages consist of evaporites (halite and anhydrite) that was deposited in lagoon environment, tectonically quiet arid and semi-arid. The marine phase it deposited siliciclastic and carbonate in the Campos Basin resulting in shales, marls, limestones, ritmito, turbidites, sandstones and others. The objective of this study is to investigate the possible continuation... (Complete abstract click electronic access below)

Análise morfológica e filogeográfica em jabutis brasileiros (Testudines)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Rubrivivax gelatinosus na alimentação de tilápias para incrementar a qualidade dos filés

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Eficiência da cokrigagem na estimativa da produtividade do café conilon

The objective of this research was to estimate the productivity (PRODUT) in sc/ha of coffee conilon through the technique of cokriging, using as covariate the production of humid coffee (PROD) in kg and compare the results with estimates obtained by kriging ordinary. The study was conducted in a commercial area of conilon coffee, Coffea canephora Pierre var. conilon, located in São Mateus Municipality, Espirito Santo State. For the field work was sampled the humid coffee production in a sampling grid irregular of 18.5 ha, 87 sampling points in the total. We also determined the production of dry coffee beans and coffee benefited 12% moisture, to obtain the PRODUT variable. After exploratory data analysis, which showed the correlation between variables in the order of 0.899, was performed variogram analysis. Were adjusted theoretical variograms to PROD and PRODUT and cross variogram between two variables. Finally we estimated the value of productivity, both by ordinary kriging as per cokriging. The validation of the estimation by cokriging not shows, however, significant gains in relation to validation by ordinary kriging.

Effects of irrigation on water use and water use efficiency in two fast growing Eucalyptus plantations

Eucalyptus plantations occupy almost 20 million ha worldwide and exceed 3.7 million ha in Brazil alone. Improved genetics and silviculture have led to as much as a three-fold increase in productivity in Eucalyptus plantations in Brazil and the large land area occupied by these highly productive ecosystems raises concern over their effect on local water supplies. As part of the Brazil Potential Productivity Project, we measured water use of Eucalyptus grandis x urophylla clones in rainfed and irrigated stands in two plantations differing in productivity. The Aracruz (lower productivity) site is located in the state of Espirito Santo and the Veracel (higher productivity) site in Bahia state. At each plantation, we measured stand water use using homemade sap flow sensors and a calibration curve using the clones and probes we utilized in the study. We also quantified changes in growth, leaf area and water use efficiency (the amount of wood produced per unit of water transpired). Measurements were conducted for 1 year during 2005 at Aracruz and from August through December 2005 at Veracel. Transpiration at both sites was high compared to other studies but annual estimates at Aracruz for the rainfed treatment compared well with a process model calibrated for the Aracruz site (within 10%). Annual water use at Aracruz was 1394 mm in rainfed treatments versus 1779 mm in irrigated treatments and accounted for approximately 67% and 58% of annual precipitation and irrigation inputs respectively. Increased water use in the irrigated stands at Aracruz was associated with higher sapwood area, leaf area index and transpiration per unit leaf area but there was no difference in the response of canopy conductance with air saturation deficit between treatments. Water use efficiency at the Aracruz site was also not influenced by irrigation and was similar to the rainfed treatment. During the period of overlapping measurements, the response to irrigation treatments at the more productive Veracel site was similar to Aracruz. Stand water use at the Veracel site totaled 975 mm and 1102 mm in rainfed and irrigated treatments during the 5-month measurement period respectively. Irrigated stands at Veracel also had higher leaf area with no difference in the response of canopy conductance with air saturation deficit between treatments. Water use efficiency was also unaffected by irrigation at Veracel. Results from this and other studies suggest that improved resource availability does not negatively impact water use efficiency but increased productivity of these plantations is associated with higher water use and should be given consideration during plantation management decision making processes aimed at increasing productivity. Published by Elsevier B.V.

Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing

The objectives were to evaluate the reexpansion blastocoele rate, post-thaw viability, and in vitro development of canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol (GLY) or 1.5 m ethylene glycol (EG). Fifty-one in vivo-produced canine blastocysts were randomly allocated in two groups: GLY (n = 26) and EG (n = 25). After thawing, embryos from MO were immediately stained with the fluorescent probes propidium iodide and Hoechst 33 342 to evaluate cellular viability. Frozen-thawed embryos from M3 and M6 were cultured in SOFaa medium + 10% FCS at 38.5 degrees C under an atmosphere of 5% CO2 with maximum humidity, for 3 and 6 days, respectively, and similarly stained. The blastocoele reexpansion rate (24 h after in vitro culture) did not differ between GLY (76.5%) and EG (68.8%). Post-thaw viable cells rate were not significantly different between GLY and EG (66.5 +/- 4.8 and 57.3 +/- 4.8, respectively, mean +/- SEM), or among MO (62.3 +/- 5.7%), M3 (56.9 +/- 6.0%), and M6 (66.5 +/- 6.0%). In conclusion, canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol or 1.5 m ethylene glycol, had satisfactory blastocoele reexpansion rates, similar post-thawing viability, and remained viable for up to 6 days of in vitro culture. (C) 2012 Elsevier Inc. All rights reserved.

Two new species of Mezilaurus (Lauraceae) from Brazil

Ongoing taxonomic work on Mezilaurus has revealed two new species of the genus in Brazil, which are here described and illustrated. The first one, Mezilaurus glabriantha, from the state of Espirito Santo, represents the third endemic species known from the Brazilian Atlantic Forest, and Mezilaurus microphylla occurs in seasonally dry forests from the central Brazilian state of Tocantins. A key to distinguish the extra-Amazonian species of Mezilaurus from Brazil is provided.

Variable expressivity of osteogenesis imperfecta in a Brazilian family due to p.G1079S mutation in the COL1A1 gene

Osteogenesis imperfecta (OI) is a Mendelian disease with genetic heterogeneity characterized by bone fragility, recurrent fractures, blue sclerae, and short stature, caused mostly by mutations in COL1A1 or COL1A2 genes, which encode the pro-alpha 1(I) and pro-alpha 2(I) chains of type I collagen, respectively. A Brazilian family that showed variable expression of autosomal dominant OI was identified and characterized. Scanning for mutations was carried out using SSCP and DNA sequence analysis. The missense mutation c.3235G>A was identified within exon 45 of the COL1A1 gene in a 16-year-old girl diagnosed as having OI type I; it resulted in substitution of a glycine residue (G) by a serine (S) at codon 1079 (p.G1079S). The proband's mother had the disease signs, but without bone fractures, as did five of nine uncles and aunts of the patient. All of them carried the mutation, which was excluded in four healthy brothers of the patient's mother. This is the first description in a Brazilian family with OI showing variable expression; only one among seven carriers for the c.3235G>A mutation developed bone fractures, the most striking clinical feature of this disease. This finding has a significant implication for prenatal diagnosis in OI disease.

The red-billed curassow (Crax blumenbachii): social organization, and daily activity patterns

We studied the Red-billed Currasow (Cray blumenbachii) in Vale Natural Reserve, Linhares, Espirito Santo State, Brazil, using camera traps. We found the Red-billed Curassow present in the entire area of the reserve (similar to 22,000 ha) during 40 months of camera trapping (2005-2008). Most records were of single individuals, especially males, but pairs and even groups of individuals were also recorded. Males were paired with one and two females, suggesting polygyny in the species. The species was recorded throughout the day with one peak from 0500 to 0600 hrs and another after 1600 hrs. The daily activity pattern was similar for males and females. The number and widespread nature of the records suggests the local population of this species may be higher than previously thought. Received 10 March 2011. Accepted 5 December 2011.