Cromatografia de troca-iônica aplicada ao isolamento da fração ácida do óleo de copaíba (Copaifera multijuga) e da sacaca (Croton cajucara)

Plant extracts are usually complex mixtures which contain several molecules of different sizes with varied functional groups. Such extracts are a challenge to the chemist of natural products. Ion exchange chromatography in non-aqueous medium, used for separation of basic or acidic fractions from plant extracts, is an important unit operation in preparative scale separations. Anionic macroporous resin in non-aqueous medium was used with success in this study for separation of the acid fraction of Copaifera multijuga (Copaiba oil), rich in labdanic diterpenes and for the methanolic extract of Croton cajucara (acetyl aleuritoric acid).

Thermische Modellierung einer Mehrlagenplatine

In der Leistungselektronik spielt die Kenntnis des Wärmevrhaltens einer Platine eine sehr große Rolle. Die immer größeren Leistungsdichten unterstreichen die Wichtigkeit des Kenntnisses des Wärmeverhaltens. In der Platine funktionieren die Leistungskomponenten und Kupferlagen die große Ströme tragen als Leistungsquellen. Das Isolationsmaterial zwischen den Kupferlagen limitiert die maximale Temperatur der Platine. Dieses bringt eine Grentzung für den maximalen Strom der durch die Platine geführt werden kann. In dieser Arbeit wurden die maximalen Stromdichten im Worst-Case-Szenario einer Platine untersucht. Dafür wurde eine Testplatine entworfen und für die Testplatine ein thermisches Modell konstruiert. Die Effekte von Kühlung wurden auch untersucht. Die Bestimmtheit des Modells wurde mit Messungen überprüft.

Kunnossapidon nykytila metsäteollisuuden voimalaitoksissa

Tämän diplomityön tavoitteena oli kartoittaa kunnossapidon nykytilaa metsäteollisuuden voimalaitoksissa. Asiaa selvitettiin kvalitatiivisena tutkimuksena, jossa haastateltiin kunnossapidon vastuuhenkilöitä. Tutkimuksen kohteeksi valittiin kahdeksan erikokoista voimalaitosta, jotka edustivat suurimpia Suomessa toimivia metsäteollisuuden konserneja. Työssä käsiteltiin tiedon ja materiaalin hallintaa, kunnossapidon suunnittelua ja organisointia sekä taloudellisia vaikutuksia. Tulokset on esitetty yleisessä muodossa ilman laitoskohtaista erittelyä. Metsäteollisuus toimii hyvin globaaleilla markkinoilla, joilla kilpailu on kiristynyt viime vuosina. Tehokkuutta on pyritty parantamaan kaikilla sektoreilla ja tämä näkyy muutoksena monilla kunnossapidon osa alueilla. Merkittävä muutos oli kunnossapitotoimintojen eriyttäminen omaksi yhtiöksi, joka oli toteutettu yhtiön strategisena valintana puolella tehtaista. Tutkimuksessa kävi myös ilmi, että henkilökunnan koulutukseen panostamalla tietojärjestelmiä voidaan hyödyntää tehokkaammin. Varastoon sidotun pääoman pienentäminen on merkinnyt materiaalilogistiikan tärkeyden korostumista tavoiteltaessa parempaa käyttövarmuutta. Kun lisäksi teollisuuden investointien määrät ovat viime vuosina laskeneet, on kunnossapidon ja etenkin sen suunnittelun rooli kasvanut.

Precipitação seletiva de tamanhos em nanopartículas semicondutoras coloidais de CdTe e CdSe: um estudo por espectroscopia UV-VIS

The post-preparative size-selective precipitation technique was applied in CdTe and CdSe semiconductor nanocrystals prepared via colloidal route in water. The synthesis of CdTe and CdSe nanoparticles and the effect of the post-preparative size-selective precipitation have been characterized mainly by mean of ultraviolet and visible absorption spectroscopy (UV-Vis). It was demonstrated that the size-selective precipitation are able to isolate particles of different sizes and purify the nanoparticles as well.

Synthesis of indium tin oxide nanoparticles by a nonhydrolytic sol-gel method

Indium tin oxide nanoparticles were synthesized in two different sizes by a nonhydrolytic sol-gel method. These powders were then transformed into ITO via an intermediate metastable state at between 300 and 600 ºC. The presence of characteristic O-In-O and O-Sn-O bands at 480 and 670 cm-1 confirmed the formation of ITO. The X-ray diffraction patterns indicated the preferential formation of metastable hexagonal phase ITO (corundum type) as opposed to cubic phase ITO when the reflux time was less than 3 h and the heat treatment temperature was below 600 ºC. Particle morphology and crystal size were examined by scanning electron microscopy.

An undergraduate level experiment on the synthesis of Au nanoparticles and their size-dependent optical and catalytic properties

The synthesis of gold nanoparticles (Au NPs) 15, 26, and 34 nm in diameter, followed by the investigation of their size-dependent optical and catalytic properties, is described herein as an undergraduate level experiment. The proposed experiment covers concepts on the synthesis, stabilization, and characterization of Au NPs, their size-dependent optical and catalytic properties at the nanoscale, chemical kinetics, and the role of a catalyst. The experiment should be performed by groups of two or three students in three lab sessions of 3 h each and organized as follows: i) synthesis of Au NPs of different sizes and investigation of their optical properties; ii) evaluation of their catalytic activity; and iii) data analysis and discussion. We believe that this activity enables students to integrate these multidisciplinary concepts in a single experiment as well as to become introduced/familiarized with an active research field and current literature in the areas of nanoparticle synthesis and catalysis.

Leaf blight and defoliation of Eugenia spp. caused by Cylindrocladium candelabrum and C. spathiphylli in Brazil

Leaf blight and defoliation of Eugenia stipitata Mc Vaugh and Eugenia patrisii Vahl, caused respectively by Cylindrocladium candelabrum (Calonectria scoparia) and C. spathiphylli (Calonectria spathiphylli) are reported in the state of Pará, Brazil. On both host species, the disease is characterized by dark brown lesions of different sizes and shapes. A whitish bright sporulation, resembling Cylindrocladium is observed on the necrotic lesions by using a stereomycroscope or a pocket lense (10-20 X). Under favorable conditions and depending on the level of infection, intense premature tree defoliation may also be found.Although the conidial germination and mycelial growth were higher at 25ºC for both species, C. candelabrum was more sensitive to the variation of temperature (10, 20, 30 and 40 ºC) than C. spathiphylli. This is the first report of C. candelabrum and C. spathiphylli on Eugenia stipitata (araçá-boi) and on Eugenia patrisii (ubaia-da-amazônia), respectively in Brazil.

Enhancement of water removal in the press section

The effectiveness of water removal on press section is very important for a paper and board machine’s functioning, efficiency and economy. Today, the most effective method for wet pressing is shoe press technology. Metso has carried out a number of studies concerning anew type of water removal method for a press section, which has also been patented. These studies include rough sketches and some test runs. These issues form the basis of this thesis. The objective of this work was to gather together information for a new and enhanced waterremoval method for a press section by studying the functioning of the device andcarrying out test runs. This method is referred to here as Hydronip. The main goal was tobuild a functional test site which fulfills all the necessary requirements and has all thenecessary information gathering devices. The design process was carried out by emphasizing the safety aspects. The goal was also to gather together information about the nip structure in running conditions, the seal function, and to carry out the nip tests with paper or board wads. This thesis consists of a theory part, the design and construction of the test site, and carrying out test runs through information gathering. The theory part consists of the principals of water removal from a press section, Hydronip construction, and the requirements for the test place. The safety aspects were taken into account especially in test runs, but also in the assembly stages. The design and construction of the test site includes the selection of equipment and surroundings that are needed for managing the test runs in the best possible way at certain premises. The test site included the equipment that was already on the premises. Some equipment could be used as it was but some equipment had to be manufactured or modified from existing equipment. A functional test site with information gathering devices was accomplished as a result of thethesis. Test runs demonstrated that the Hydronip concept is, at least on a small scale,functional. Short-term tests for seal functioning showed that the seal can be lubricatedsufficiently under different kinds of nip load situations. Wad tests demonstrated that the metal belt is durable against different sizes of external particles. The seal also endured wad tests even though the pressure impacts impaired the lubrication. MTS tests showing dry content increases, combined with a rough cost calculation and the basic function of the machine in test runs, show that with some further study Hydronip could be a promising new product for water removal from a paper or board machine’s press section.

Germination of Caesalpinia leiostachya (benth.) ducke seeds classified by size and shape

Caesalpinia leiostachya (Benth.) Ducke (Caesalpiniaceae) is a Brazilian legumenous tree whose seeds present different sizes and shapes, with dormancy imposed by a waterproof coat. In order to improve that quality of lot, seeds were classified by width in sieves of circular meshes (13, 14, 15, 16 and 17) and manually separated by shape in elongate, round and angular. Masses of 1000 seeds were determined and the thickness of each width and shape seedclass was measured, and coat permeability of the seeds classified by the width was investigated. Seeds were scarified in concentrated sulphuric acid and submitted to the germination test. Both final percentage and speed of germination index were evaluated. The classification of the seeds improved the quality of the lot, and the coat permeability was not affected by seed width. Elongated-flat seeds are of low physiological quality and should be discarded. Masses of 1000 seeds, percentage final and speed of germination increased with the width of the seeds. Both elongated and angular seeds had similar germinative behavior, and round seeds are of greater thickness and of superior physiological quality.

Development of an automated bench top electronic penetrometer

The aim of this study was to develop a an automated bench top electronic penetrometer (ABEP) that allows performing tests with high rate of data acquisition (up to 19,600 Hz) and with variation of the displacement velocity and of the base area of cone penetration. The mechanical components of the ABEP are: a supporting structure, stepper motor, velocity reducer, double nut ball screw and six penetration probes. The electronic components of ABEP are: a "driver" to control rotation and displacement, power supply, three load cells, two software programs for running and storing data, and a data acquisition module. This penetrometer presented in compact size, portable and in 32 validation tests it proved easy to operate, and showed high resolution, high velocity in reliability in data collection. During the validation tests the equipment met the objectives, because the test results showed that the ABEP could use different sizes of cones, allowed work at different velocities, showed for velocity and displacement, were only 1.3% and 0.7%, respectively, at the highest velocity (30 mm s-1) and 1% and 0.9%, respectively for the lowest velocity (0.1 mm s-1).

Influence of the sample area in the variability of losses in the mechanical harvesting of soybeans

The mechanical harvesting is an important stage in the production process of soybeans and, in this process; the loss of a significant number of grains is common. Despite the existence of mechanisms to monitor these losses, it is still essential to use sampling methods to quantify them. Assuming that the size of the sample area affects the reliability and variability between samples in quantifying losses, this paper aimed to analyze the variability and feasibility of using different sizes of sample area (1, 2 and 3 m²) in quantifying losses in the mechanical harvesting of soybeans. Were sampled 36 sites and the cutting losses, losses by other mechanisms of the combine and total losses were evaluated, as well as the water content in seeds, straw distribution and crop productivity. Data were subjected to statistical analysis (descriptive statistics and analysis of variance) and Statistical Control Process (SCP). The coefficients of variation were similar for the three frames available. Combine losses showed stable behavior, whereas cutting losses and total losses showed unstable behavior. The frame size did not affect the quantification and variability of losses in the mechanical harvesting of soybeans, thus a frame of 1 m² can be used for determining losses.

Teollisten palveluiden kehittäminen asiakaslähtöisesti

Teollisuuden palvelut tuottavat tulevaisuudessa yhä suuremman osan yritysten liikevaihdosta ja tämän vuoksi niiden systemaattinen kehittäminen yrityksessä on erityisen tärkeää. Tutkimuksen tavoitteena on luoda teorian pohjalta malli, jonka avulla mekatroniikkaklusterin yritykset voivat analysoida tarjottavia palveluitaan asiakkaan näkökulmasta. Tutkimuksessa pyritään myös selvittämään empiirisesti mallin soveltuvuus palvelutarjonnan kehittämiseen tutkimuksessa mukana oleville erikokoisille yrityksille. Tutkimuksen teoreettisessa osassa luodaan katsaus yrityksen kasvustrategioihin. Palveluiden innovaatio- sekä kehitysprosessit käydään lyhyesti läpi, sekä esitetään lähtökohtia, miten yritykset voivat siirtyä tuotetarjoajasta palveluntarjoajaksi. Teoriaosassa käydään läpi QFD -malli, jota voidaan käyttää hyödyksi kun analysoidaan asiakkaan palvelutarpeita ja priorisoidaan yrityksen resursseja. QFD -mallin lisäksi esitellään myös muita laatutyökaluja sekä asiakkaan tarpeiden selvitysmenetelmiä, joita yritys voi käyttää hyödykseen QFD -mallin kanssa. Synteesiosassa esitellään ensin MS Excel -pohjainen QFD -malli. Mallin käyttö-kokeilu toteutetaan työpaja tyylisessä kokouksessa, ja tutkimustulokset kerätään havainnoimalla, teemahaastattelulla sekä kyselyn avulla.

Väsymiskestävyyden määritys murtumismekaanisilla särönmallinnusohjelmilla

Tässä työssä tutkitaan Wärtsilä Oyj:n moottorin ja ABB Oy:n generaattorin muodostaman aggregaatin väsymiskestävyyttä lineaarisen murtumismekaniikan avulla. Työn tavoitteena on selvittää ABAQUS/XFEM- ja FRANC3D-ohjelman soveltuvuus kuormitukseltaan ja geometrialtaan vaativan generaattorirungon suunnittelutyökaluksi. Generaattorirungon kuormitukset aiheutuvat aggregaatin käynnin sekä käynnistys- ja sammutusvaiheen aikai-sista syntyvistä värähtelyistä. Tutkimuksessa tarkastellaan generaattorirungon väsymistä käynninaikaisella kuormituksella. Työssä mallinnettiin generaattorirungosta valittu hitsausdetalji alimallinnustekniikalla, jolloin alimallin reunaehdot voitiin määrittää aggregaatille tehdyn vastelaskennan perus-teella. Alimallista tutkittiin kahta erilaista hitsiliitostyyppiä, joihin mallinnettiin XFEM- ja FRANC3D-ohjelmilla erikokoisia säröjä hitsiliitosten rajaviivalle sekä juuren puolelle. Tutkittavilla ohjelmilla saatujen jännitysintensiteettikertoimien avulla säröille voitiin las-kea ekvivalentti jännitysintensiteettikerroin, jota verrattiin kokeellisesti saatuun jännitysin-tensiteettikertoimen kynnysarvoon. XFEM- ja FRANC3D-ohjelmia vertailtiin käytön helppouden, tulosten tarkkuuden sekä laskenta-aikojen perusteella. Käytettävyyden ja laskenta-aikojen perusteella XFEM-ohjelma soveltui paremmin käytettäväksi teollisuudessa suunnittelu- ja kehitystyön apu-työkaluna. FRANC3D taas antoi XFEM-ohjelmaa luotettavampia tuloksia, mutta laskenta-ajat olivat moninkertaiset.

Nerve endings of filliform, fungiform and vallate papillae of dorsal tongue mucosa of White-lipped peccary (Tayassu pecari): Neurohistological observations

The neurohistologic observations were performed using the specimens prepared by Winkelmann and Schmitt silver impregnation method. The tissues were fixed in 10% formalin solution and sections of 40µm thickness were obtained by Leica Cryostat at -30ºC. The sections of dorsal mucosa of White-lipped peccary tongue showed numerous filliform and fungiform papillae, and two vallate papillae on the caudal part. The epithelial layer revealed queratinized epithelial cells and the connective tissue papillae of different sizes and shapes. Thick nerve fiber bundles are noted into the subepithelial connective tissue of the papillae. The connective tissue of fungiform and vallate papillae contained numerous sensitive nerves fibers bundles forming a complex nerve plexus.

Ultrastructural lesions in the myocardium and kidneys of rabbits in experimental acute Amorimia exotropica poisoning

Abstract: Amorimia exotropica is an important plant associated with sudden death in cattle in Southern Brazil. In order to understand the mechanisms by which A. exotropica causes acute lesions in the heart and kidney of intoxicated animals, an experiment was conducted to determine the histopathology and ultrastructure of myocardial and renal lesions of intoxicated rabbits. After receiving 18g/kg of dried plant, six rabbits died suddenly. At necropsy, the liver was swollen and no other macroscopic lesions were observed. Histologically, centrolobular and midzonal hepatocytes were vacuolated. These vacuoles were strong PAS stained positive, suggesting that they corresponded to glycogen accumulations. In some regions of the ventricular septum and ventricles were found vacuoles of different sizes and the kidneys of two rabbits showed vacuolar degeneration on distal convoluted tubules. Ultrastructurally, the myocardium had cardiomyocytes swelling with separation of myofibrils bundles and rupture and disorganization of the sarcomeres. The mitochondria displayed swelling, disorganization, disruption of the mitochondrial cristae, and electron-dense matrix. Some mitochondria exhibited eccentric projections of their membranes with disruption of both outer and inner membranes. The sarcoplasmic reticulum had no alterations, whereas the T-tubule system was occasionally dilated and ruptured. The kidneys had mitochondrial swelling with disorganization and disruption of the mitochondrial cristae. The vacuoles result from the swelling of the endoplasmatic reticulum and usually were located between two basolateral infoldings and mitochondria, occurring preferentially around the nucleus. The myocytes and T system damages induced by A. exotropica result in acute heart failure and death. Furthermore, this mechanism of cardiotoxicity may be common to all plant containing monofluoroacetate.