Effect of low-fluoride dentifrices supplemented with calcium glycerophosphate on enamel demineralization in situ

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise da penetração de material resinoso ao esmalte dental em função de agente clareador e tempo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Resistência à cárie de esmalte dental irradiado por lasers de Er:YAG e Nd:YAG, avaliada através da morfologia e proporção Ca/P

Pós-graduação em Ciências Odontológicas - FOAR

Efeito do uso de vernizes com alta e baixa concentração de fluoreto suplementado com trimetafosfato sobre a erosão e abrasão do esmalte dentário: estudo in vitro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito de leites fermentados contendo probióticos sobre o esmalte bovino e no biofilme dental formado in situ

Pós-graduação em Ciência Odontólogica - FOA

Efeito do uso de dentifricios com alta e baixa concentração de fluoreto suplementado com trimetafosfato sobre a erosão e abrasão do esmalte dentário bovino: estudo in vitro

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Ação de géis fluoretados, suplementados ou não com hexametafosfato de sódio, no processo de erosão do esmalte dentário: estudo in situ

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência do clareamento dental na cor, translucidez e fluorescência do esmalte e dentina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação das alterações de cor e análise da composição mineral do esmalte dental bovino após clareamento com peróxido de hidrogênio utilizando fotorreflectância e espectroscopia FT-raman

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Resistência ao microcisalhamento de diferentes sistemas adesivos no esmalte dental humano

Pós-graduação em Odontologia Restauradora - ICT

Influência de diferentes íons sobre a erosão dentária

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

In situ protocol for the detemination of dose-response effect of low-fluoride dentifrices on enamel remineralization

No in situ protocol has assessed the dose-response effects of fluoride dentifrices involving low-fluoride formulations. Objective: To assess the ability of an in situ remineralization model in determining dose-response effects of dentifrices containing low fluoride concentrations ([F]) on bovine enamel. Material and Methods: Volunteers wore palatal appliances containing demineralized enamel blocks and brushed their teeth and devices with the dentifrices supplied (double-blind, crossover protocol) separately for 3 and 7 days. Surface hardness (SH), integrated subsurface hardness (AKHN) and [F] in enamel were determined. Data were analyzed by ANOVA, Tukey's test and Pearson's correlation (p<0.05). Results: Dose-response relationships were verified between [F] in dentifrices and SH, AKHN and enamel [F]. Higher correlation coefficients between enamel [F] and SH and AKHN were obtained for the 3-day period. Significant differences in SH and AKHN were observed among all groups for the 3-day period, but not between 0-275, 275-550, and 550-1,100 mu g F/g dentifrices for the 7-day period, nor between 3- and 7-day periods for the 1,100 mu g F/g groups. Conclusions: Considering that the peak remineralization capacity of the conventional dentifrice (1,100 mu g F/g) was achieved in 3 days, this experimental period could be used in future studies assessing new dentifrice formulations, especially at low-fluoride concentrations.

Effect of low-fluoride toothpastes combined with hexametaphosphate on in vitro enamel demineralization

Objectives: The aim of this study was to evaluate the anticaries effect of low-fluoride toothpastes combined with hexametaphosphate (HMP) on enamel demineralization.Methods: Bovine enamel blocks were subjected to pH cycling and treatment with toothpaste's slurries (15 groups; 2x/day). Toothpaste mixtures contained the following: no fluoride (F) plus HMP (from 0 to 3.0%); 250 ppm F plus HMP (from 0 to 3.0%); 500 ppm F; 1100 ppm F; and a commercial toothpaste (1100 ppm F). After pH cycling, surface and cross-sectional hardness, as well as F present in the enamel were determined. The demineralization depth was analyzed using polarized light microscopy. The variables were subjected to 1-way ANOVA, followed by Student-Newman-Keuls' test (p < 0.05).Results: In the absence of fluoride, 0.5% HMP promoted the lowest mineral loss and its effect was similar to that of a 250 ppm F toothpaste (p > 0.05). The combination of 0.5% HMP and 250 ppm F resulted in lower mineral loss (p < 0.05) and similar lesion depth when compared to the 1100 ppm F toothpaste (p > 0.05).Conclusion: To conclude, the combination of 0.5% HMP and 250 ppm fluoride in a toothpaste has a similar inhibitory effect on enamel demineralization in vitro when compared to a toothpaste containing 1100 ppm F.Clinical significance: The anticaries effect of toothpaste containing 250 ppm F combined with 0.5% HMP was similar to that of a 1100 ppm F toothpaste, despite the 4-fold difference in F concentration. Although such effects still need to be demonstrated in clinical studies, it may be a viable alternative for preschool children. (C) 2013 Elsevier Ltd. All rights reserved.

Erosion Protection by Calcium Lactate/Sodium Fluoride Rinses under Different Salivary Flows in vitro

This study investigated the effect of a calcium lactate pre-rinse on sodium fluoride protection in an in vitro erosion-remineralization model simulating two different salivary flow rates. Enamel and dentin specimens were randomly assigned to 6 groups (n = 8), according to the combination between rinse treatments - deionized water (DIW), 12 mm NaF (NaF) or 150 mm calcium lactate followed by NaF (CaL + NaF) and unstimulated salivary flow rates - 0.5 or 0.05 ml/min simulating normal and low salivary flow rates, respectively. The specimens were placed into custom-made devices, creating a sealed chamber on the specimen surface connected to a peristaltic pump. Citric acid was injected into the chamber for 2 min, followed by artificial saliva (0.5 or 0.05 ml/min) for 60 min. This cycle was repeated 4x/day for 3 days. Rinse treatments were performed daily 30 min after the 1st and 4th erosive challenges, for 1 min each time. Surface loss was determined by optical profilometry. KOH-soluble fluoride and structurally bound fluoride were determined in specimens at the end of the experiment. Data were analyzed by 2-way ANOVA and Tukey tests (alpha = 0.05). NaF and CaL + NaF exhibited significantly lower enamel and dentin loss than DIW, with no difference between them for normal flow conditions. The low salivary flow rate increased enamel and dentin loss, except for CaL + NaF, which presented overall higher KOH-soluble and structurally bound fluoride levels. The results suggest that the NaF rinse was able to reduce erosion progression. Although the CaL prerinse considerably increased F availability, it enhanced NaF protection against dentin erosion only under hyposalivatory conditions. (C) 2014 S. Karger AG, Basel

Bonding durability of dental sealants to deciduous and permanent teeth

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)