Biologia de Neotrioza tavaresi Crawford, 1925 (Hemiptera, Psyllidae), galhador da folha do araçazeiro (Psidium cattleianum)

Biology of the leaf gall inducer Neotrioza tavaresi Crawford, 1925 (Hemiptera, Psyllidae) on strawberry guava tree (Psidium cattleianum). A field study was conducted in Curitiba region, State of Paraná, southern Brazil, to describe the life cycle of Neotrioza tavaresi Crawford, 1925, a leaf galling insect in strawberry guava trees (Psidium cattleianum). Three cycles were observed (1997, 1998, 1999) during regular field trips and the insects were observed in Piraquara municipality, where 15 samples with 50 infested leaves were sampled in the 1997-98 cycle. Galls were dissected for detailed studies. Neotrioza tavaresi has a univoltine cycle in which adult individuals were found inside the galls from August onwards. The sexually mature insects with sex ratio 1, emerged from the galls after their dehiscence caused by feeding of the adult insects on the gall walls. Adult emergence started in early October and ended by early December, with its peak in November. Copulation took place as soon as adults exit the gall and egg laying started the next day. Females had more than 100 ovarioles containing 218.7±44.7 (n=50) fully formed eggs. This indicated the short sexual adult life-span (aprox. 5-7 days) of the species, also characterized by a concentrated oviposition. Adult individuals fed and laid their eggs on younger shoots of the plant. The bottoms of the yellowish eggs were inserted into the leaf tissue, mainly on its adaxial edge (78.1%). The nymphs hatched and, as they fed on the adaxial side of expanding leaves, modified the cell growth pattern and the round-shape galls developed on the adaxial side with one insect inside. The gall wall showed distinct layers, with the inner one suppliyng the food to the insects, and the outer layer supplying gall protection. Nymphs went through five instars and the exuviae remained stuck on a ball of wax inside the gall. All parasitoids found were Hymenoptera belonging to Chalcidoidea: Eulophidae (1 sp), Pteromalidae (2 spp) and Encyrtidae (3 spp). The findings suggest that leaf gall inducer and parasitoids insects and plant life cycles are closely connected and both leaf sprouting and gall opening seem to be triggered by the same environmental and plant conditions. The high abundance of shoots may favor insect performance as adult individuals can easily find an ideal place for feeding, copulating and laying eggs.

Conservation of oceanic island floras: Present and future global challenges

Currentthreatstotheplanet’sbiodiversityareunprecedented,andtheyparticularlyimperilinsular floras.Inthisinvestigation,weusethethreatfactorsidentifiedbytheMillenniumEcosystem Assessmentasthemaindriversofbiodiversitylossonislandstodefineandrank13current,continuing threatstotheplantdiversityofninefocalarchipelagoswherevolcanicorigin(orintheSeychellesa prolongedisolationafteracontinentalorigin)hasproducedahighdegreeofendemicityandfragilityin the faceofhabitatalteration.Wealsoconductaglobalendangermentassessmentbasedonthe numbersofinsularendemicplantsintheendangered(EN)andcriticallyendangered(CR)IUCN categoriesfor53islandgroupswithanestimated9951endemicplantspecies,providinga representativesampleoftheworld’sinsularsystemsandtheirfloristicrichness.Ouranalysesindicate that isolationdoesnotsignificantlyinfluenceendangerment,butplantendemicsfromverysmall islandsaremoreoftencriticallyendangered.Weestimatethatbetween3500and6800oftheestimated 70,000 insularendemicplantspeciesworldwidemightbehighlythreatened(CR+EN)andbetweenca. 2000 and2800ofthemincriticaldangerofextinction(CR).Basedontheseanalyses,andona worldwideliteraturereviewofthebiologicalthreatfactorsconsidered,weidentifychallenging questionsforconservationresearch,asking(i)whatarethemosturgentprioritiesfortheconservation of insularspeciesandfloras,and(ii)withtheknowledgeandassetsavailable,howcanweimprovethe impactofconservationscienceandpracticeonthepreservationofislandbiodiversity?Ouranalysis indicatesthatthesynergisticactionofmanythreatfactorscaninducemajorecologicaldisturbances, leadingtomultipleextinctions.Wereviewweaknessesandstrengthsinconservationresearchand managementintheninefocalarchipelagos,andhighlighttheurgentneedforconservationscientiststo shareknowledgeandexpertise,identifyanddiscusscommonchallenges,andformulatemulti- disciplinaryconservationobjectivesforinsularplantendemicsworldwide.Toourknowledge,thisisthe mostup-to-dateandcomprehensivesurveyyettoreviewthethreatfactorstonativeplantsonoceanic islandsanddefinepriorityresearchquestions.

Audit report on the Crawford County Area Solid Waste Agency Commission for the year ended June 30, 2007

Audit report on the Crawford County Area Solid Waste Agency Commission for the year ended June 30, 2007

Audit report on the Crawford County Area Solid Waste Agency Commission for the year ended June 30, 2008

Audit report on the Crawford County Area Solid Waste Agency Commission for the year ended June 30, 2008

Biological characteristics of Telenomus alecto and Trichogramma galloi reared on eggs of the sugarcane borer Diatraea flavipennella

Diatraea flavipennella (Box) (Lepidoptera, Crambidae) is one of the most destructive pests in sugarcane plantations in the Northeast Region of Brazil. Developmental characteristics and parasitism potential of the egg parasitoids Telenomus alecto Crawford (Hymenoptera, Scelionidae) and Trichogramma galloi Zucchi (Hymenoptera, Trichogrammatidae) were compared with the aim of selecting a suitable species for biological control of D. flavipennella. Both T. alecto and T. galloi developed well and were readily adapted to D. flavipennella eggs as host. Although, T. galloi presented higher viability, with more adults emerging per host egg and higher sex ratio, the developmental period (egg-adult) was shorter in T. alecto and female longevity was extended. In addition, T. alecto exhibited significant higher levels of parasitism during the first three days after emergence than T. galloi. Thus, both parasitoids studied here offer considerable potential for the control of D. flavipennella in sugarcane.

The major genetic determinants of HIV-1 control affect HLA class I peptide presentation.

Infectious and inflammatory diseases have repeatedly shown strong genetic associations within the major histocompatibility complex (MHC); however, the basis for these associations remains elusive. To define host genetic effects on the outcome of a chronic viral infection, we performed genome-wide association analysis in a multiethnic cohort of HIV-1 controllers and progressors, and we analyzed the effects of individual amino acids within the classical human leukocyte antigen (HLA) proteins. We identified >300 genome-wide significant single-nucleotide polymorphisms (SNPs) within the MHC and none elsewhere. Specific amino acids in the HLA-B peptide binding groove, as well as an independent HLA-C effect, explain the SNP associations and reconcile both protective and risk HLA alleles. These results implicate the nature of the HLA-viral peptide interaction as the major factor modulating durable control of HIV infection.

Egg parasitoids of Edessa meditabunda (Fabricius) (Pentatomidae) in lettuce crop

Egg parasitoids of Edessa meditabunda (Fabricius) (Pentatomidae) in lettuce crop. This study evaluated the occurrence of parasitoids in eggs of the stink bug, Edessa meditabunda (Fabricius, 1794) (Heteroptera, Pentatomidae) found on lettuce crop in Mato Grosso State, Brazil. Samples were collected in the vegetable garden "Cheiro Verde", in the municipality of Tangará da Serra, in curly lettuce. A bed of lettuce was selected randomly, where the eggs of E. meditabunda were collected. Five-hundred and seventy eight eggs of E. meditabunda were collected, 86.3% of them parasitized by Telenomus podisi Ashmead, 1893 (Hymenoptera, Platygastridae), Trissolcus urichi Crawford, 1913 (Hymenoptera, Platygastridae) and Neorileya albipes Girault, 1913 (Hymenoptera, Eurytomidae), representing respectively 57.0%, 38.2% and 4.8% of the emerged individuals. This is the first record of these three species parasitizing eggs of E. meditabunda in lettuce.

A photometric analysis of the Cepheus OB3 region

Results are presented on Stromgren-Crawford uvby-beta photometry carried out at Calar Alto (Spain) for 45 stars in the Cepheus OB3 region covering an apparent area of 6 deg x 6 deg. The relationship of these stars with the association is examined. Three of these stars (BD +62 deg 2114, BD +62 deg 2152, and BD +62 deg 2156) are identified as members of this association, while two more (BD +64 deg 1714 and BD +64 deg 1717) are classified as possible members. It is noted that intrinsic colors and absolute magnitudes of member stars are consistent with the hypothesis of Blaauw (1964) and Garmany (1973) of the existence of two subgroups with different evolutionary phases.

Audit report on the Crawford County Area Solid Waste Agency Commission for the year ended June 30, 2009

Audit report on the Crawford County Area Solid Waste Agency Commission for the year ended June 30, 2009

Audit report on the Crawford County Area Solid Waste Agency Commission for the year ended June 30, 2010

Audit report on the Crawford County Area Solid Waste Agency Commission for the year ended June 30, 2010

Biological, clinical and population relevance of 95 loci for blood lipids.

Plasma concentrations of total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol and triglycerides are among the most important risk factors for coronary artery disease (CAD) and are targets for therapeutic intervention. We screened the genome for common variants associated with plasma lipids in >100,000 individuals of European ancestry. Here we report 95 significantly associated loci (P < 5 x 10(-8)), with 59 showing genome-wide significant association with lipid traits for the first time. The newly reported associations include single nucleotide polymorphisms (SNPs) near known lipid regulators (for example, CYP7A1, NPC1L1 and SCARB1) as well as in scores of loci not previously implicated in lipoprotein metabolism. The 95 loci contribute not only to normal variation in lipid traits but also to extreme lipid phenotypes and have an impact on lipid traits in three non-European populations (East Asians, South Asians and African Americans). Our results identify several novel loci associated with plasma lipids that are also associated with CAD. Finally, we validated three of the novel genes-GALNT2, PPP1R3B and TTC39B-with experiments in mouse models. Taken together, our findings provide the foundation to develop a broader biological understanding of lipoprotein metabolism and to identify new therapeutic opportunities for the prevention of CAD.

Audit report on the Crawford County Area Solid Waste Agency Commission for the year ended June 30, 2011

Audit report on the Crawford County Area Solid Waste Agency Commission for the year ended June 30, 2011

The Inaccuracy of National Character Stereotypes

Consensual stereotypes of some groups are relatively accurate, whereas others are not. Previous work suggesting that national character stereotypes are inaccurate has been criticized on several grounds. In this article we (a) provide arguments for the validity of assessed national mean trait levels as criteria for evaluating stereotype accuracy and (b) report new data on national character in 26 cultures from descriptions (N = 3323) of the typical male or female adolescent, adult, or old person in each. The average ratings were internally consistent and converged with independent stereotypes of the typical culture member, but were weakly related to objective assessments of personality. We argue that this conclusion is consistent with the broader literature on the inaccuracy of national character stereotypes.

Audit report on the Crawford County Area Solid Waste Agency Commission for the year ended June 30, 2012

Audit report on the Crawford County Area Solid Waste Agency Commission for the year ended June 30, 2012

An integrated encyclopedia of DNA elements in the human genome.

The human genome encodes the blueprint of life, but the function of the vast majority of its nearly three billion bases is unknown. The Encyclopedia of DNA Elements (ENCODE) project has systematically mapped regions of transcription, transcription factor association, chromatin structure and histone modification. These data enabled us to assign biochemical functions for 80% of the genome, in particular outside of the well-studied protein-coding regions. Many discovered candidate regulatory elements are physically associated with one another and with expressed genes, providing new insights into the mechanisms of gene regulation. The newly identified elements also show a statistical correspondence to sequence variants linked to human disease, and can thereby guide interpretation of this variation. Overall, the project provides new insights into the organization and regulation of our genes and genome, and is an expansive resource of functional annotations for biomedical research.