Lack of functional alpha-lactalbumin prevents involution in Cape fur seals and identifies the protein as an apoptotic milk factor in mammary gland involution

The mammary gland undergoes a sophisticated programme of developmental changes during pregnancy/lactation. However, little is known about processes involving initiation of apoptosis at involution following weaning. We used fur seals as models to study the molecular process of involution as these animals display a unique mammary gland phenotype. Fur seals have long lactation periods whereby mothers cycle between secreting copious quantities of milk for 2 to 3 days suckling pups on land, with trips to sea alone to forage for up to 23 days during which time mammary glands remain active without initiating apoptosis/involution.

Perspectives of sporting opportunities for remote indigenous Cape York communities

The health outcomes for Indigenous peoples are well publicised as being poorer than that of the rest of the Australian population. The importance of physical activity as part of a balanced approach to health and wellbeing are well documented. Physical inactivity is a significant risk factor for many preventable diseases that many non-Indigenous, but specifically more Indigenous peoples die from. A recent report on Indigenous health indicated that only 23% of adults living in remote and very remote areas, such as Cape York, participated in regular physical activity. Physical activity initiatives in remote Indigenous communities on Cape York are commonly delivered by external agencies that ‘fly in and fly out’. While members of Indigenous communities may engage with the initiatives while they are being provided once the external agencies leave some of the benefits made may be quickly lost. There is no current published literature on the variety, prevalence and outcomes of ‘fly-in fly-out’ physical activity programs, or on the agencies that provide them. An understanding of these factors would facilitate a better understanding of the opportunities available to Indigenous communities on Cape York and provide important foregrounding to an investigation of community capacity for physical activity. The purpose of this study was to investigate the range of physical activity programs being offered by external agencies to Indigenous Cape York communities.

Methods: Five physical activity agencies that routinely engaged with Indigenous communities on Cape York were interviewed. The semi-structured interviews focussed on what activities were being conducted; by whom; when; and their concomitant outcomes. Interviews were recorded and professionally transcribed. Transcriptions were then analysed using content analysis to identify themes.

Results: Each physical activity agency had a variety of ways of engaging with community. The key initial focus point for each provider was the local school. Contacts within the school and opportunities to provide workshop opportunities for the students then facilitated wider community engagement.

Discussion: There were limited opportunities for these agencies to build community capacity to maintain their physical activities due to a variety of reasons that included: resources (both human and material); transient populations and an entrenched culture of ‘having things done to’ rather than with Indigenous people. In order to improve the physical activity outcomes of Indigenous people on Cape York community’s strategies that engage and empower the local population to take control of their needs should be employed.

A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation

The colostrum trypsin inhibitor (CTI) gene and transcript were cloned from the Cape fur seal mammary gland and CTI identified by in silico analysis of the Pacific walrus and polar bear genomes (Order Carnivora), and in marine and terrestrial mammals of the Orders Cetartiodactyla (yak, whales, camel) and Perissodactyla (white rhinoceros). Unexpectedly, Weddell seal CTI was predicted to be a pseudogene. Cape fur seal CTI was expressed in the mammary gland of a pregnant multiparous seal, but not in a seal in its first pregnancy. While bovine CTI is expressed for 24-48h postpartum (pp) and secreted in colostrum only, Cape fur seal CTI was detected for at least 2-3months pp while the mother was suckling its young on-shore. Furthermore, CTI was expressed in the mammary gland of only one of the lactating seals that was foraging at-sea. The expression of β-casein (CSN2) and β-lactoglobulin II (LGB2), but not CTI in the second lactating seal foraging at-sea suggested that CTI may be intermittently expressed during lactation. Cape fur seal and walrus CTI encode putative small, secreted, N-glycosylated proteins with a single Kunitz/bovine pancreatic trypsin inhibitor (BPTI) domain indicative of serine protease inhibition. Mature Cape fur seal CTI shares 92% sequence identity with Pacific walrus CTI, but only 35% identity with BPTI. Structural homology modelling of Cape fur seal CTI and Pacific walrus trypsin based on the model of the second Kunitz domain of human tissue factor pathway inhibitor (TFPI) and porcine trypsin (Protein Data Bank: 1TFX) confirmed that CTI inhibits trypsin in a canonical fashion. Therefore, pinniped CTI may be critical for preventing the proteolytic degradation of immunoglobulins that are passively transferred from mother to young via colostrum and milk.

A Visibility Analysis of the Cape Wind Project

Cape Wind has proposed a wind farm of 130 turbines on Horseshoe Shoal in the center of Nantucket Sound. A prominent concern about the project is the impact the visibility of the turbines will have on the region's tourism industry and property values. It is feared that their presence will diminish the value of the pristine coastline that has attracted vacationers to Cape Cod for generations. In this project, we assess the extent to which Cape Cod, Martha's Vineyard, and Nantucket will be visually affected by the wind farm. It was completed using a Viewshed Analysis in the GIS program, ArcMap, from the surface, mean, and maximum height of the towers. These Viewsheds were combined to give a comprehensive perspective of which areas are able to see the highest percent of the wind farm. Finally, a weighted land use value was applied to the Viewshed to account for the impact of land use on the ability to see the project. The objective of this analysis is to provide a visual representation of how great an influence the wind farm will in fact have on Cape Cod.

The historic religious buildings of Ribeira Grande: implementation of christian models in the early colonies, 15th till 17th century, on the example of Cape Verde Islands

Rui Carita