Li-Fraumeni syndrome fibroblasts homozygous for p53 mutations are deficient in global DNA repair but exhibit normal transcription-coupled repair and enhanced UV resistance.

We investigated whether mutations in the p53 tumor suppressor gene alter UV sensitivity and/or repair of UV-induced DNA damage in primary human skin fibroblasts from patients with Li-Fraumeni syndrome, heterozygous for mutations in one allele of the p53 gene (p53 wt/mut) and sublines expressing only mutant p53 (p53 mut). The p53 mut cells were more resistant than the p53 wt/mut cells to UV cytotoxicity and exhibited less UV-induced apoptosis. DNA repair analysis revealed reduced removal of cyclobutane pyrimidine dimers from overall genomic DNA in vivo in p53 mut cells compared with p53 wt/mut or normal cells. However, p53 mut cells retained the ability to preferentially repair damage in the transcribed strands of expressed genes (transcription-coupled repair). These results suggest that loss of p53 function may lead to greater genomic instability by reducing the efficiency of DNA repair but that cellular resistance to DNA-damaging agents may be enhanced through elimination of apoptosis.

Replication protein A binds to regulatory elements in yeast DNA repair and DNA metabolism genes.

Saccharomyces cerevisiae responds to DNA damage by arresting cell cycle progression (thereby preventing the replication and segregation of damaged chromosomes) and by inducing the expression of numerous genes, some of which are involved in DNA repair, DNA replication, and DNA metabolism. Induction of the S. cerevisiae 3-methyladenine DNA glycosylase repair gene (MAG) by DNA-damaging agents requires one upstream activating sequence (UAS) and two upstream repressing sequences (URS1 and URS2) in the MAG promoter. Sequences similar to the MAG URS elements are present in at least 11 other S. cerevisiae DNA repair and metabolism genes. Replication protein A (Rpa) is known as a single-stranded-DNA-binding protein that is involved in the initiation and elongation steps of DNA replication, nucleotide excision repair, and homologous recombination. We now show that the MAG URS1 and URS2 elements form similar double-stranded, sequence-specific, DNA-protein complexes and that both complexes contain Rpa. Moreover, Rpa appears to bind the MAG URS1-like elements found upstream of 11 other DNA repair and DNA metabolism genes. These results lead us to hypothesize that Rpa may be involved in the regulation of a number of DNA repair and DNA metabolism genes.

Types of work performed using resurfacing, restoration, rehabilitation, and reconstruction federal highway funds : report to the chairman, Subcommittee on Investigations and Oversight, Committee on Public Works and Transportation, U.S. House of Representatives /

"B-212809"--Prelim. p. [1].

Consumer expenditure survey series, interview survey, 1972 and 1973 : average annual expenditures for motor vehicle purchases, maintenance, repair, and operations, selected data, 1972 and 1973.

Mode of access: Internet.

Social-economic movements; an historical and comparative survey of socialism, communism, co-operation, utopianism; and other systems of reform and reconstruction

Selected references":p. 753-806.

Characteristics of vehicle repair and maintenance problems for current vehicle-in-use. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Issues and innovations in library buildings : renovation and reconfiguration /

"April 1999"--Verso.

Diagnostic motor vehicle inspection demonstration projects, program engineering support. Volume 9 - appendix H: vehicle designs that facilitate or hinder inspection and repair and interchangeability and interface capability of test equipment and vehicles. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Auto repair and maintenance - programs to reduce consumer loss.

Mode of access: Internet.

Era of the oath; Northern loyalty tests during the Civil War and reconstruction.

Bibliography: p. [208]-222.

Rivers and harbors bill, 1948. Hearings before the Subcommittee on Rivers and Harbors of the Committee on Public Works, House of Representatives, Eightieth Congress, second session, on H.R. 6419 (Title I) authorizing the construction, repair, and preservation of certain public works on rivers and harbors for navigation, flood control, and for other purposes. February 11, 12, March 8, 11, 24, April 5, 8, 19, 30, 1948.

"No. 18."

Applications of precast concrete in repair and replacement of civil works structures /

Mode of access: Internet.

The Civil war and Reconstruction in Florida [electronic resource] /

Reproduced from Studies in history, economics, and public law, vol. 53, whole no. 131.