Content Marketing: O Poder dos Conteúdos no Marketing Relacional – Estudo Aplicado ao Mercado de Bricolage

A presente investigação pretende demonstrar o poder dos conteúdos no processo de construção de relações entre marcas e consumidores. Se por um lado visa compreender de que forma é que a comunicação integrada de conteúdos – Content Marketing – pode ser uma ferramenta essencial para a empresa ganhar notoriedade, posicionamento e consequentemente receitas, por outro propõese analisar o papel do Content Marketing como uma das dimensões do Marketing Relacional. O estudo foca-se no mercado de bricolage, direccionado para a marca Leroy Merlin. Foi utilizado o método quantitativo através de inquéritos por questionário a uma amostra de 80 consumidores de bricolage em Portugal. Os resultados permitem concluir que no mercado estudado a qualidade intangível é a variável do marketing relacional com impacto na satisfação de clientes, sendo que para efeitos de fidelização, as variáveis significativas são: o compromisso, os factores do meio-envolvente e a notoriedade. O marketing de conteúdos revelou-se pouco significativo para a obtenção de satisfação e fidelização de clientes, no entanto apresentou um impacto indirecto através da notoriedade que depois terá relação com a satisfação e com a fidelização.

Clinical presentation, etiology, and outcome of infective endocarditis in the 21st century: the International Collaboration on Endocarditis-Prospective Cohort Study.

We sought to provide a contemporary picture of the presentation, etiology, and outcome of infective endocarditis (IE) in a large patient cohort from multiple locations worldwide. Prospective cohort study of 2781 adults with definite IE who were admitted to 58 hospitals in 25 countries from June 1, 2000, through September 1, 2005. The median age of the cohort was 57.9 (interquartile range, 43.2-71.8) years, and 72.1% had native valve IE. Most patients (77.0%) presented early in the disease (<30 days) with few of the classic clinical hallmarks of IE. Recent health care exposure was found in one-quarter of patients. Staphylococcus aureus was the most common pathogen (31.2%). The mitral (41.1%) and aortic (37.6%) valves were infected most commonly. The following complications were common: stroke (16.9%), embolization other than stroke (22.6%), heart failure (32.3%), and intracardiac abscess (14.4%). Surgical therapy was common (48.2%), and in-hospital mortality remained high (17.7%). Prosthetic valve involvement (odds ratio, 1.47; 95% confidence interval, 1.13-1.90), increasing age (1.30; 1.17-1.46 per 10-year interval), pulmonary edema (1.79; 1.39-2.30), S aureus infection (1.54; 1.14-2.08), coagulase-negative staphylococcal infection (1.50; 1.07-2.10), mitral valve vegetation (1.34; 1.06-1.68), and paravalvular complications (2.25; 1.64-3.09) were associated with an increased risk of in-hospital death, whereas viridans streptococcal infection (0.52; 0.33-0.81) and surgery (0.61; 0.44-0.83) were associated with a decreased risk. In the early 21st century, IE is more often an acute disease, characterized by a high rate of S aureus infection. Mortality remains relatively high.

TRPM1 is mutated in patients with autosomal-recessive complete congenital stationary night blindness.

Night vision requires signaling from rod photoreceptors to adjacent bipolar cells in the retina. Mutations in the genes NYX and GRM6, expressed in ON bipolar cells, lead to a disruption of the ON bipolar cell response. This dysfunction is present in patients with complete X-linked and autosomal-recessive congenital stationary night blindness (CSNB) and can be assessed by standard full-field electroretinography (ERG), showing severely reduced rod b-wave amplitude and slightly altered cone responses. Although many cases of complete CSNB (cCSNB) are caused by mutations in NYX and GRM6, in approximately 60% of the patients the gene defect remains unknown. Animal models of human diseases are a good source for candidate genes, and we noted that a cCSNB phenotype present in homozygous Appaloosa horses is associated with downregulation of TRPM1. TRPM1, belonging to the family of transient receptor potential channels, is expressed in ON bipolar cells and therefore qualifies as an excellent candidate. Indeed, mutation analysis of 38 patients with CSNB identified ten unrelated cCSNB patients with 14 different mutations in this gene. The mutation spectrum comprises missense, splice-site, deletion, and nonsense mutations. We propose that the cCSNB phenotype in these patients is due to the absence of functional TRPM1 in retinal ON bipolar cells.

Temporal changes in mRNA expression of the brain nutrient transporters in the lithium-pilocarpine model of epilepsy in the immature and adult rat.

The lithium-pilocarpine model mimics most features of human temporal lobe epilepsy. Following our prior studies of cerebral metabolic changes, here we explored the expression of transporters for glucose (GLUT1 and GLUT3) and monocarboxylates (MCT1 and MCT2) during and after status epilepticus (SE) induced by lithium-pilocarpine in PN10, PN21, and adult rats. In situ hybridization was used to study the expression of transporter mRNAs during the acute phase (1, 4, 12 and 24h of SE), the latent phase, and the early and late chronic phases. During SE, GLUT1 expression was increased throughout the brain between 1 and 12h of SE, more strongly in adult rats; GLUT3 increased only transiently, at 1 and 4h of SE and mainly in PN10 rats; MCT1 was increased at all ages but 5-10-fold more in adult than in immature rats; MCT2 expression increased mainly in adult rats. At all ages, MCT1 and MCT2 up-regulation was limited to the circuit of seizures while GLUT1 and GLUT3 changes were more widespread. During the latent and chronic phases, the expression of nutrient transporters was normal in PN10 rats. In PN21 rats, GLUT1 was up-regulated in all brain regions. In contrast, in adult rats GLUT1 expression was down-regulated in the piriform cortex, hilus and CA1 as a result of extensive neuronal death. The changes in nutrient transporter expression reported here further support previous findings in other experimental models demonstrating rapid transcriptional responses to marked changes in cerebral energetic/glucose demand.

Research resource: the dynamic transcriptional profile of sertoli cells during the progression of spermatogenesis.

Sertoli cells (SCs), the only somatic cells within seminiferous tubules, associate intimately with developing germ cells. They not only provide physical and nutritional support but also secrete factors essential to the complex developmental processes of germ cell proliferation and differentiation. The SC transcriptome must therefore adapt rapidly during the different stages of spermatogenesis. We report comprehensive genome-wide expression profiles of pure populations of SCs isolated at 5 distinct stages of the first wave of mouse spermatogenesis, using RNA sequencing technology. We were able to reconstruct about 13 901 high-confidence, nonredundant coding and noncoding transcripts, characterized by complex alternative splicing patterns with more than 45% comprising novel isoforms of known genes. Interestingly, roughly one-fifth (2939) of these genes exhibited a dynamic expression profile reflecting the evolving role of SCs during the progression of spermatogenesis, with stage-specific expression of genes involved in biological processes such as cell cycle regulation, metabolism and energy production, retinoic acid synthesis, and blood-testis barrier biogenesis. Finally, regulatory network analysis identified the transcription factors endothelial PAS domain-containing protein 1 (EPAS1/Hif2α), aryl hydrocarbon receptor nuclear translocator (ARNT/Hif1β), and signal transducer and activator of transcription 1 (STAT1) as potential master regulators driving the SC transcriptional program. Our results highlight the plastic transcriptional landscape of SCs during the progression of spermatogenesis and provide valuable resources to better understand SC function and spermatogenesis and its related disorders, such as male infertility.

Traité de l'orthographe franc̜oise, en forme de dictionaire. Tome 1 / , enrichi de notes critiques et de remarques sur l'étymologie et la prononciation des mots, le genre des noms, la conjugaison des verbes irréguliers et les variations, avec une préface grammaticale, un Traité de versification franc̜oise et un Dictionnaire géographique. Par P. Restaut. Nouvelle édition revue, corrigée, augmentée et prosodiée d'après les principes d'Olivet... Par Claude-Félix Roger... Tome I. A-K [-II. L-Z]

Comprend : Abrégé du traité de l'orthographe géographique ; Abrégé des regles de la versification franc̜oise

Traité de l'orthographe franc̜oise, en forme de dictionaire. Tome 2 / , enrichi de notes critiques et de remarques sur l'étymologie et la prononciation des mots, le genre des noms, la conjugaison des verbes irréguliers et les variations, avec une préface grammaticale, un Traité de versification franc̜oise et un Dictionnaire géographique. Par P. Restaut. Nouvelle édition revue, corrigée, augmentée et prosodiée d'après les principes d'Olivet... Par Claude-Félix Roger... Tome I. A-K [-II. L-Z]

Comprend : Abrégé du traité de l'orthographe géographique ; Abrégé des regles de la versification franc̜oise

Recueil de poésies.

Contient : 1 Louanges du roy Charlles neufvieme de se non ; 2 Pièce en deux huitains ; 3 « Elegie sur le despart de la reyne Marye » [par RONSART] ; 4 « Adieus de madame DE CRUSSOL », en treize couplets adressés, « A la reyne ma mere », — « au roy mon filz », — « à la reyne ma fillie », — « à Messieurs », — « à monsieur le cardinal de Lorraine », — « à monsieur de Guyse », — à monsieur le cardinal de Guyse », — « à monsieur le prince de Ginville », — « à monsieur de Nevers », — « à monsieur le mareschal de Sainct André », — « à ma fillie de Guyse », — « à madame de Montpencyer », — « à la Contive » ; 5 Chansons ; « Souventes foys en ces lieux... » ; « Estre loyal je ne puys... » ; « Qui pourra dire la doulleur... » ; « Amour va conme le vent... » ; « Hélas ! mon Dieu, quand aures vous mercy... » ; « Si j'ay deux serviteurs... » ; « Escoutes toutz la plaincte... » ; « Pelerin suys d'un voyage... » ; 6 « Epistre » ; 7 « Quatre Epistres escriptes par quatre damoyselles à quatre gentilzhonmes de diverses affections » ; « Responce » ; « Epistre de la seconde damoyselle » ; « Responce » ; « Epistre de la tierce damoyselle » ; « Responce » ; « Epistre de la quatriesme » : ; « Responce » ; 8 « Epistre » [d'ELISABETH DE FRANCE à Catherine de Medicis?] ; 9 Dialogue entre une femme, quatre filles et un homm ; 10 Stances de quatre vers ; 11 Dizain ; 12 Chanson ; 13 Diverses pièces ; 14 Épître de Catherine de Médicis au roi d'Espagne ; 15 Épîtres ; « La royne à madame Isabel, raine d'Espagne » ; « Madame à la royne » : ; « La royne à Madame » ; « Madame à la reyne » ; « La royne à Madame » : ; « La royne à Madame » ; 16 Suite de trente-neuf dizains ; 17 Pièces adressées « par le herault de Venus à la royne », — « à Madame », — « à madame la duchesse de Valentinois », — « à madame d'Aumalle », — « à madame la mareschalle de La Marche », — « à madame la mareschalle de Sainct André », — « à mademoyselle de Chantelou », — « à mademoyselle de... » ; 18 « Epistre de la royne de Navarre au roy » ; 19 Neuf petites pièces, dizains ou huitains ; 20 Chanson ; 21 Epîtres ; 22 Dizain ; 23 Chanson ; 24 « Appocalipse du Pasquil », suite de treize quatrains sur « leroy », — « le pappe », — « l'empereur », — « le sennechalle », — le « connestable », — « Vendosme », — « madame Marguerite », — l'«Angleterre», — l'«admiral », — « Estampes », — « Orace et la bastarde », — les « dames de la court », — « l'autheur » ; 25 Autres quatrains, où on fait parler : « le roy », — « la royne », — « la royne Alyenor », — « madame Marguerite », — « le roy de Navarre », — « la princesse de Navarre », — « le cardinal de Bourbon », — « la royne de Navarre », — « monsieur de Vendosme », — « monsieur d'Anguyen », — etc ; 26 « Epitaphe sur le trespas de tres excellent seigneur monseigneur Just de Tournon, conte de Rossillon » : ; 27 Huitain, servant d'envoi à l'épitaphe précédente, et adressé « à tres excelant et magnanime seigneur monseigneur de Tournon, conte de Rossillon » ; 28 Sonnets ; 29 « Plaisant recit faict par Callymache, poette grec, du dieu Mars et de la deesse Latonne, mis du latin en poesie françoyse, par JEHAN DE LA MAISON NEUFVE » : ; 30 Sonnets ; 31 « Odde à tres noble et excellent seigneur monseigneur le conte de Roussillion » : ; 32 « Cantique sur la louange des lettres et vertus, presenté à monseigneur le conte de Rossillon » : ; 33 Diverses pièces ; 34 « Dialogue de deux amantz » :