Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male × Colossoma macropomum female) (Osteichthyes)

Este estudo avaliou a resposta inflamatória aguda induzida por injeções de 0,5 mL de solução salina (controle), 500 µg de carragenina e 0,5 mL de tioglicolato a 3% na bexiga natatória de juvenis do híbrido tambacu. Os peixes foram distribuídos em três tratamentos, três repetições e aclimatados durante 10 dias antes do ensaio. A caracterização das células do exsudato inflamatório foi feita após coloração com Giemsa e PAS. Peixes injetados com carragenina apresentaram maior número de células no exsudato inflamatório do que com salina e tioglicolato. A porcentagem de trombócitos no exsudato foi maior nos injetados com carragenina quando comparada com a dos injetados com tioglicolato. Por outro lado, o percentual de granulócitos foi maior em animais injetados com tioglicolato do que em animais injetados com carragenina. A carragenina provocou maior migração de macrófagos para o foco inflamatório. O método de PAS confirmou a presença de três tipos de granulócitos: célula granular eosinofílica (CGE) tipo 1 com as características da célula granulocítica especial encontrada no sangue, CGE tipo 2, menor do que esta última, e de neutrófilos. Este estudo contribui para o melhor entendimento da resposta inflamatória e dos processos infecciosos em peixes nativos.

Avaliação histofuncional de matriz heteróloga acelular como scaffold para células de músculo liso para implante em uretra de coelhos

Pós-graduação em Bases Gerais da Cirurgia - FMB

Estudos estruturais por dinâmica molecular do peptídeo Polybia-MPI via Replica Exchange

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Contribuição do estudo ultrassonográfico (Modo e Doppler) de órgãos abdominais em gatos do mato (Leopardus tigrinus) híbridos: valores de referência

Pós-graduação em Medicina Veterinária - FMVZ

Repercussões do tabagismo passivo durante a prenhez e associado ao exercício físico durante o crescimento na musculatura de ratos wistar

Pós-graduação em Fisioterapia - FCT

Influência da fixação e de métodos de secagem na detecção de alterações proliferativas ultraestruturais induzidas pelo Diuron no urotélio vesical de ratos wistar

Pós-graduação em Patologia - FMB

REPARAÇÃO DE HÉRNIA PERINEAL EM CÃES COM PERITÔNIO DE BOVINO CONSERVADO EM GLICERINA

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise comparativa da expressão de RNAm de aquaporinas em sapos hidratados e submetidos ao estresse hídrico

A maior parte das espécies de anuros adultos terrestres está constantemente exposta a altas taxas de perda de água por evaporação através da pele. A manutenção do balanço hídrico nestes animais envolve a absorção de água através da mancha pélvica, uma região da pele ventral altamente permeável à água, além da reabsorção de água a partir do fluido filtrado nos glomérulos ao longo do sistema tubular dos néfrons, bem como a partir da urina formada e estocada na bexiga urinária, em resposta à arginina vasotocina (AVT). O movimento de água através da membrana plasmática ocorre através de poros formados por proteínas integrais de membrana, conhecidas como aquaporinas (AQPs), e a regulação osmótica exercida pelo AVT envolve translocação de vesículas contendo AQPs do citoplasma para a membrana apical e, provavelmente, alteração na expressão de alguns tipos de AQPs. Resultados previamente obtidos no laboratório demonstraram a existência de variação interespecífica nas taxas de reidratação de três espécies de Rhinella, sendo que R. ornata apresentou taxas de reidratação significativamente menores que aquelas apresentadas por R. schneideri e R. icterica. Uma possível explicação para esta variação em taxas de reidratação poderia envolver diferenças na expressão de aquaporinas na mancha pélvica. Desta forma, o objetivo do projeto foi identificar e quantificar a expressão do RNAm de aquaporinas do tipo 1 (AQP-1) e de aquaporinas AVT dependentes pertencentes ao tipo 2a (AQP-t2 e AQP-t3) - quanto à classificação das AQPs entenda-se AQP -t aquelas que apresentam sequência gênica referentes a Rinella marina AQP -h a Hyla japonica.- nos seguintes tecidos: pele dorsal e pele ventral (mancha pélvica) de espécimes de R. schneideri e R. ornata totalmente hidratados e após submissão à desidratação correspondente a 70% da massa corpórea padrão. As hipótese ...

Expansão ex vivo de células uroteliais em scaffolds 3D

Malformations and possible damages to the urogenital system can be originated in the embryonic period. Moreover, fire guns, knives and accidents, where there is the disruption of the urethra, also cause these lesions. The objective was to analyze the contribution of tissue engineering in the construction of neo-urethra, developed by bioengineering. We performed an urothelial ex vivo expansion of cells in 3D scaffolds (platelet gel matrix and acellular porcine aorta) to assess the contribution of this technique in the construction of a neo-urethra. Mechanical dissociation was made of the inner wall of 10 North Folk rabbit’s bladder, weighing 2.5 to 3.0 kg. After dissociation the cell content was centrifuged and obtained a pellet of urothelial cells. The pellet was ressuspended in culture medium DMEM F12 and cells were maintained in culture for 15 days. Immunohistochemical analysis characterized the urothelial culture. The cells were then implanted in the scaffold - platelet gel. In a second experiment using aortic porcine acellular matrix were implanted urothelial cells alone and urothelial cells on platelet gel, on the inner wall of the scaffold - aorta, with space for setting bordered by a urethral probe. The complex probe - cells - aorta and probe - cells in platelet gel - aorta, were sealed with suture material and culture were maintained in a humidified 37ºC incubator with 5% CO2 in air for 12 days to subsequent histological analysis of urothelium cell adhesion to the scaffolds. By observation under an optical microscope, we could see the growth of cells in the scaffold platelet gel, from a monolayer in to a three-dimensional structure. In the acellular porcine aortic matrix containing the platelet gel, we could observe a few quantity of urothelial cells adhered. However with the acellular porcine aortic matrix in which was implanted only the urothelial cells, we have obtained adhesion to the wall

Principais aspectos cirúrgicos da urolitíase em cães

Urolithiasis occurs with relatively frequency among the diseases that affect the urinary tract of dogs. It`s fundamental that samples of the uroliths are sent for analysis, because, in agreement with the mineral composition of the urolith and also its location, the choice is made between the surgical treatment or the clinical dissolution. Cystotomy is the surgical technique more accomplished in the bladder of small animals, due to its simplicity, when in comparison with other procedures for removal of calculi. With the aid of lithotripsy and endoscopy, the surgical interventions in the urinary treatment tend to be less and less invasive, minimizing the risks of subsequent complications. Chances of recurrence of the urolithiasis are usually larger in patients submitted to the surgical intervention. Besides, the time elapsed among regressive episodes is larger after the clinical dissolution

Análise histomorfométrica do músculo cardíaco de ratos recém natos gerados por matrizes submetidas ao tabagismo passivo associado ao exercício

Maternal smoking during pregnancy causes heart changes, impaired fetal growth, spontaneous abortion and low birth weight. Even in pregnant women exposed to cigarette smoke these changes can be observed. But the exercise is to answer the increased fetal weight, besides the improvement of cardiorespiratory fitness of the pregnant and the fetus. The objective of this study was to analyze the ventricular muscle of young rats subjected to passive smoking associated with aquatic exercise. For the experiments used 24 female rats were divided into: GC (control), GE (exercise protocol in the water), GF(exposed to cigarette smoke) and GEF (exercise protocol on water and exposed to cigarette smoke). On the same day was held the 1st session of the experimental phase of the protocol of exposure to cigarette smoke, which consisted of 30 minutes twice a day, six days a week for three weeks, followed by the first session of the swimming program... (Complete abstract click electronic access below)

Dopplerfluxometria e avaliação morfométrica do fígado, baço, rins e grandes vasos abdominais de onças pardas (Puma concolor) da região Campo Grande, Mato Grosso do Sul, Brasil

Pós-graduação em Biotecnologia Animal - FMVZ

Avaliação dos efeitos de aloe-emodin em células uroteliais imortalizadas e neoplásicas cultivadas in vitro

Aloe vera (Aloe barbadensis Miller), popularly known in Brazil as babosa, has a long history of use as medicinal plant for different therapeutic purposes. The components of the plant extract are present in various products of human use, mainly for nutritional and cosmetics purposes. However, some studies suggest that this extract might also have carcinogenic activity. The aloe vera extract is a complex mixture of bioactive compounds. The study of isolated compounds may contribute to elucidate contradictory results about the effects related to the consumption of the plant, as well as their mechanisms of action. One of the most important compound from Aloe vera is aloe-emodin, which is a secondary metabolite generated in the intestinal tract. Putative antimicrobial and antitumor effects were previously attributed to aloe-emodin. Although the exposure of urothelial cells to aloe-emodin was already reported in the literature, only one study showed its effects on urothelial cells, suggesting that aloe-emodin inhibits the viability of T24 cancer cells due to apoptosis induction. Since there is no sufficient information about the effects of aloe-emodin on urothelial cells, and low efficiency in the treatment of bladder cancer currently, the present study aims to evaluate the hypothesis that the treatment with aloe-emodin could impact the behavior of other urothelial cell lines in vitro. Therefore, the in vitro IC50 exposure of aloe-emodin to human immortalized neoplastic urothelial cells will be determinated in order to verify possible differences in the behavior of urothelial cells in vitro treated with aloe-emodin in comparison with untreated cells. Furthermore, differences between cell lines will be also evaluated

Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury

Cell therapy has frequently been reported as a possible treatment for spinal trauma in humans and animals; however, without pharmacologically curative action on damage from the primary lesion. In this study, we evaluated the effect of administering human adipose-derived stem cells (hADSC) in rats after spinal cord injury. The hADSC were used between the third and fifth passages and a proportion of cells were transduced for screening in vivo after transplantation. Spinal cord injury was induced with a Fogarty catheter no. 3 inserted into the epidural space with a cuff located at T8 and filled with 80 mu L saline for 5 min. The control group A (n = 12) received culture medium (50 mu L) and group B (n = 12) received hADSC (1.2 x 10(6)) at 7 and 14 days post-injury, in the tail vein. Emptying of the bladder by massage was performed daily for 3 months. Evaluation of functional motor activity was performed daily until 3 months post-injury using the Basso-Beattie-Bresnahan scale. Subsequently, the animals were euthanized and histological analysis of the urinary bladder and spinal cord was performed. Bioluminescence analysis revealed hADSC at the application site and lungs. There was improvement of urinary bladder function in 83.3% animals in group B and 16.66% animals in group A. The analysis of functional motor activity and histology of the spinal cord and urinary bladder demonstrated no significant difference between groups A and B. The results indicate that transplanted hADSC improved urinary function via a telecrine mechanism, namely action at a distance.

Alterações ultrassonográficas renais e vesicais de ovinos confinados e suplementados com cloreto de amônio

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)