Inibição da mineralização de compostos nitrogenados de lixiviado de macrófita aquática: Adequação metodológica

This study aimed to establish the adequate concentrations of nitrapyrin for the effective inhibition of oxygen demand required for the oxidation of nitrogen compounds during aerobic mineralization of leachate. The aquatic macrophyte Myriophyllum aquaticum was used as a source of dissolved organic matter (DOM). The leachate was incubated in several chambers, containing different composition and treatments: (i) treatment with leachate (DOM), (ii) treatment with DOM plus nitrapyrin in different concentrations (10; 25 and 50 mg L-1), aiming at finding the right concentration that inhibits the nitrification process, (iii) treatment with DOM plus NH4SO 2, (iv) treatment containing DOM and nitrapyrin plus NH 4SO2, and (v) treatment with DOM plus azide. The dissolved oxygen amount in each incubation treatment was periodically measured. The results indicated that the ratio of 10 mg L-1 of nitrapyrin for each 10 mg C of leachate is efficient in inhibiting the oxidation of nitrogen compounds from aquatic macrophytes.

Glicerol-3-Fosfato oxidase em levedura de panificação

Pós-graduação em Alimentos e Nutrição - FCFAR

Glicerol quinase de levedura de panificação

Pós-graduação em Alimentos e Nutrição - FCFAR

Dosagem de etanol utilizando alcool desidrogenase de levedura de panificação

Pós-graduação em Alimentos e Nutrição - FCFAR

Polímeros de coordenação de cobre (II) contendo ligantes nitrogenados lineares: caracterização e propriedades vapocrômicas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Investigação estrutural e atividade tuberculostática de complexos mononucleares e binucleares de paládio (II)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Investigação estrutural e atividade tuberculostática de complexos de cobre(II) contendo isonicotinamida

Pós-graduação em Química - IQ

Potencial estrogênico, antimutagênico e antigenotóxico de extratos e substâncias isoladas do gênero Syngonanthus (Eriocaulaceae) e efeito inibidor na expressão de genes por xantonas de Syngonanthus nitens

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo do potencial antimutagênico, mutagênico, estrogênico e antibacteriano de flavonoides

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Novas espécies moleculares e supramoleculares de cobre (II) contendo aminas bidentadas: síntese, caracterização estrutural e investigação do comportamento vapocrômico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Vegetable Oil Derived Solvent, and Catalyst Free Click Chemistry'' Thermoplastic Polytriazoles

Azide-alkyne Huisgen click chemistry provides new synthetic routes for making thermoplastic polytriazole polymers-without solvent or catalyst. This method was used to polymerize three diester dialkyne monomers with a lipid derived 18 carbon diazide to produce a series of polymers (labelled C18C18, C18C9, and C18C4 based on monomer chain lengths) free of residual solvent and catalyst. Three diester dialkyne monomers were synthesized with ester chain lengths of 4, 9, and 18 carbons from renewable sources. Significant differences in thermal and mechanical properties were observed between C18C9 and the two other polymers. C18C9 presented a lower melting temperature, higher elongation at break, and reduced Young's modulus compared to C18C4 and C18C18. This was due to the odd-even effect induced by the number of carbon atoms in the monomers which resulted in orientation of the ester linkages of C18C9 in the same direction, thereby reducing hydrogen bonding. The thermoplastic polytriazoles presented are novel polymers derived from vegetable oil with favourable mechanical and thermal properties suitable for a large range of applications where no residual solvent or catalyst can be tolerated. Their added potential biocompatibility and biodegradability make them ideal for applications in the medical and pharmaceutical industries.

Differences in the hydroxylation pattern of flavonoids alter their chemoprotective effect against direct- and indirect-acting mutagens

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da microinfiltração marginal em restaurações de resina composta, quando o colágeno dentinário é mantido ou eliminado após o condicionamento ácido, alterando-se o período de armazenamento e a termociclagem

Pós-graduação em Odontologia Restauradora - ICT

Avaliação das atividades citotóxicas e imunomoduladora de paládio(II) contendo o ligante DMBA= N,N-dimetilbenzilamina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The importance of myeloperoxidase in apocynin-mediated NADPH oxidase inhibition

Apocynin is widely used as an inhibitor of the NADPH oxidase. Since myeloperoxidase (MPO) has been considered as essential for the mechanism of action of apocynin, here we used cells with different levels of MPO and compared their sensitivity to apocynin. HL-60 cells were differentiated with DMSO or IFN γ /TNF α and compared with peripheral mononuclear (PBMC) and polymorphonuclear cells (PMN). The relative MPO activity was PBMC = HL60 DMSO < HL60 IFN γ < PMN. Apocynin inhibited the intracellular reactive oxygen species production by PMN (80%) and IFN γ /TNF α -differentiated HL-60 cells (45%) but showed a minor effect in PBMC and DMSO differentiated HL-60 cells (20%). The addition of azide decreased the efficiency of apocynin in PMN and the addition of peroxidase increased the inhibition in PBMC. We also determined the gene expression of the components gp91phox, p47phox, p22phox and p67phox in the resting cells. Apocynin did not change gp91phox, p47phox or p22phox gene expression in nonstimulated PBMC, HL60 DMSO, HL60 IFN γ /TNF α , and PMN and has a subtle increase in p67phox in HL60 IFN γ /TNF α . The results from this work suggest that a rational search for better inhibitors of NADPH oxidase in leukocytes should include a correlation with their affinity as substrates for MPO.