Seasonal changes in Florida avocados /

Literature cited: p. 27-28.

A novel plant toxin, persin, with in vivo activity in the mammary gland, induces Bim-dependent apoptosis in human breast cancer cells

Phytochemicals have provided an abundant and effective source of therapeutics for the treatment of cancer. Here we describe the characterization of a novel plant toxin, persin, with in vivo activity in the mammary gland and a p53-, estrogen receptor-, and Bcl-2-independent mode of action. Persin was previously identified from avocado leaves as the toxic principle responsible for mammary gland-specific necrosis and apoptosis in lactating livestock. Here we used a lactating mouse model to confirm that persin has a similar cytotoxicity for the lactating mammary epithelium. Further in vitro studies in a panel of human breast cancer cell lines show that persin selectively induces a G(2)-M cell cycle arrest and caspase-dependent apoptosis in sensitive cells. The latter is dependent on expression of the BH3-only protein Bim. Bim is a sensor of cytoskeletal integrity, and there is evidence that unique structure of the compound, persin could represent a novel class of microtubule-targeting agent with potential specificity for breast cancers.

Between-tree variation in fruit quality and fruit mineral concentrations of Hass avocados

Inconsistent internal fruit quality in Hass avocados affects consumer confidence. To determine the influence of individual trees on fruit quality, Hass avocado fruit were harvested from adjacent trees of similar external appearance in 3 commercial orchards in 1998 and 1 orchard in 1999. The trees in each orchard were grown with similar commercial practices and in similar soil types. Within each location, there were significant (P < 0.05) differences in the mean ripe fruit quality between trees with respect to fruit body rot severity ( mainly anthracnose) with and without cold storage, internal disorders severity due to diffuse discolouration and vascular browning ( after cold storage), days to ripen, percentage dry matter, and the percentage of the skin area with purple-black colour when ripe. These effects were also noted in the same orchard in 1999. There were significant (P < 0.05) differences in fruit flesh calcium, magnesium, potassium, boron and zinc concentrations between trees. Significant (P < 0.05) correlations were observed between average fruit mineral concentrations in each tree ( particularly calcium, magnesium and potassium) and body rot severity, percentage dry matter and fruit mass. There was little conclusive evidence that characteristics such as the growth of the non-suberised roots or the degree of scion under- or overgrowth was involved in these tree effects; however, differences between trees with respect to other rootstock characteristics may be involved. The inconsistency of the correlations across sites and years suggested that other factors apart from tree influences could also affect the relationship between fruit minerals and fruit quality.

Farmers’ Market Nutrition Program Recipe Cards

These are cards giving recipes for fruit and vegetable dishes. Recipes include: confetti bean salsa, Mediterranean quinoa salad, minty watermelon cucumber salad, quick refrigerator pickles, broccoli salad, apple, cranberry and almond coleslaw, farmers’ market pasta salad, roasted beet and butter bean hummus, spinach and strawberry pecan salad, okra, avocado and tomato salad, plum-berry mint smoothie and crispy baked vegetables.

Desenvolvimento e crescimento da espécie de cogumelo Pleurotus ostreatus em garrafas de plástico reutilizado

Dissertação de Mestrado, Tecnologia e Segurança Alimentar, 22 de Junho de 2016, Universidade dos Açores.

ADAPTABILIDAD DE LAS FAMILIAS AGRÍCOLAS ANTE PRESIONES ECONÓMICAS Y ECOLÓGICAS: UN CASO DE CAFÉ DE ALTURA EN LEÓN CORTÉS, COSTA RICA

Resumen Los medios de vida de las familias productoras de café de altura están ligados a la finca. Esta relación tan marcada (familia – finca) los hace vulnerables a presiones externas que alteran el funcionamiento cotidiano interno de la unidad productiva. Dentro de estas presiones externas se encuentran los bajos precios, efectos climáticos y plagas en el cafetal. El artículo busca identificar los impactos que ejercen dichas presiones sobre los medios de vida de las familias cafetaleras del cantón de León Cortés, además se intenta mostrar las estrategias que han seguido estas unidades familiares para poder soportar y reponerse a los impactos generados por el clima, las plagas y la inestabilidad de precios del café. Las familias han generado diversas “estrategias de adaptación” en relación con los precios bajos, las organizaciones de productores han buscado nuevas alternativas de comercialización del café, tales como mercado justo y denominación de origen; además, han buscado la generación de un mayor valor agregado mediante la diversificación del producto final enfocado al café de calidad y a la presentación de café molido. Por su parte, las unidades familiares han buscado alternativas de diversificación como la siembra de aguacate. Para combatir las plagas los productores se han enfocado en la introducción de prácticas de conservación de suelos, mejorar sistemas de recolección e introducir sombra en los cultivos; el combate químico también ha sido una estrategia. Por último, la presión del clima no había sido muy tomada en cuenta por los caficultores de la zona, sin embargo, la Tormenta Alma sufrida en el año 2008 hizo que los caficultores comenzaran a pensar en un manejo adecuado y sostenible de la finca que minimice el riesgo climático. Abstract Livelihoods of family coffee growers are strongly determined by their farm. This makes families vulnerable to external shocks that affect the operations in the plot. Within these external shocks are included low prices, pests, and climate effects on the plantation. This paper aims to identify the impact of the aforementioned shocks on family coffee grower livelihoods in the canton of León Cortés. For this purpose, the article shows the strategies followed by families in order to support and recover themselves from the impact which come from climate, pests, and coffee price instability. Families have pursued various “adapting strategies”. Regarding low prices, producers' organizations have sought coffee marketing alternatives, such as fair trade and appellation of origin. Likewise, they work on increasing added value through diversification, improving coffee quality, and by a better packaging of ground coffee. Also, households have sought diversification options, such as avocado production. In order to combat pests, producers have focused on the introduction of soil conservation practices, improvement of collection systems, growing trees on coffee plantations, and chemical control. Finally, climate shocks had not been considered as a real problem by the farmers until the storm Alma affected the region. Nowadays, they think more about sound and sustainable management for their farm.

Farmers’ Market Nutrition Program Recipe Cards

These are cards giving recipes for fruit and vegetable dishes. Recipes include: confetti bean salsa, Mediterranean quinoa salad, minty watermelon cucumber salad, quick refrigerator pickles, broccoli salad, apple, cranberry and almond coleslaw, farmers’ market pasta salad, roasted beet and butter bean hummus, spinach and strawberry pecan salad, okra, avocado and tomato salad, plum-berry mint smoothie and crispy baked vegetables.

Bio-preservation and bio-active compounds as sustainable strategies to improve quality and safety of fresh and fermented products

The development of new “green” and sustainable approaches to reduce food wastes, guaranteeing food quality, microbiological safety and the environmental sustainability, is of great interest for food industry. This PhD thesis, as part of the European project BioProMedFood (PRIMA–Section2 Programme), was focused on two strategies: the use of natural antimicrobials and the application of microbial strains isolated from spontaneously fermented products. The first part concerned the valorisation of microbial biodiversity of 15 Mediterranean spontaneously fermented sausages, through the isolation of autochthonous lactic acid bacteria (LAB) strains, mainly Latilactobacillus sakei, that were characterised regarding their safety and technological aspects. The most promising strains were tested as bio-protective cultures in fresh sausages, showing promising anti-listerial activity, or as starter cultures in fermented sausages. The second part of the research was focused on the use of natural compounds (phenolic extracts and essential oils from Juniperus oxycedrus needles and Rubus fruticosus leaves) with antimicrobial potential. They were tested in vitro against List. monocytogenes and Enterococcus faecium, showing differences in relation to species and type of extracts, but they hint at important possibilities for applications in specific foods. Concluding, this PhD thesis highlighted the great potential of traditional meat products as an isolation source of new strains with industrial importance. Moreover, the antimicrobial potential of compounds obtained from plant matrices opened promising perspectives to exploit them as “green” strategies to increase fresh food safety. The last topic of research, carry out in collaboration with Department of Nutrition and Food Sciences (University of Granada), investigated the effect of LAB fermentation on avocado leaves by-products, focusing on the bio-availability of phenolic compounds in the plant extracts, caused by microbial metabolism.