979 resultados para Allo-suckling


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Half title: Opere edite ed inedite di Vincenzo Gioberti, v. 1-4.

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"Opera premiata dal Reale istituto lombardo di scienze e lettere."

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Each part except "Poems on several occasions" has special t.p.

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I. Del manoscritto.--II. Il libro a stampa.--III. Le biblioteche.--IV. I libri di consultazione e i periodici.--V. La storia letteraria.--VI. Raccolte di scrittori e di rime.--VII. Vocabolarii, grammatiche, metriche.--VIII. Indicazioni bibliografiche sussidiarie.--Appendici.

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Includes bibliographical references and index.

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Modification of human islets prior to transplantation may improve long-term clinical outcome in terms of diabetes management, by supporting graft function and reducing the potential for allo-rejection. Intragraft incorporation of stem cells secreting beta (β)-cell trophic and immunomodulatory factors represents a credible approach, but requires suitable culture methods to facilitate islet alteration without compromising integrity. This study employed a three-dimensional rotational cell culture system (RCCS) to achieve modification, preserve function, and ultimately influence immune cell responsiveness to human islets. Islets underwent intentional dispersal and rotational culture-assisted aggregation with amniotic epithelial cells (AEC) exhibiting intrinsic immunomodulatory potential. Reassembled islet constructs were assessed for functional integrity, and their ability to induce an allo-response in discrete T-cell subsets determined using mixed islet:lymphocyte reaction assays. RCCS supported the formation of islet:AEC aggregates with improved insulin secretory capacity compared to unmodified islets. Further, the allo-response of peripheral blood mononuclear cell (PBMC) and purified CD4+ and CD8+ T-cell subsets to AEC-bearing grafts was significantly (p < 0.05) attenuated. Rotational culture enables pre-transplant islet modification involving their integration with immunomodulatory stem cells capable of subduing the allo-reactivity of T cells relevant to islet rejection. The approach may play a role in achieving acute and long-term graft survival in islet transplantation.

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tesi riguardante la progettazione di un brick asettico